Amino acid dipepetide frequency for Halothiobacillus sp. LS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.352AlaAla: 13.352 ± 0.179
0.988AlaCys: 0.988 ± 0.037
6.436AlaAsp: 6.436 ± 0.098
6.933AlaGlu: 6.933 ± 0.118
3.761AlaPhe: 3.761 ± 0.069
8.64AlaGly: 8.64 ± 0.141
2.693AlaHis: 2.693 ± 0.057
5.848AlaIle: 5.848 ± 0.092
3.351AlaLys: 3.351 ± 0.082
12.96AlaLeu: 12.96 ± 0.154
2.976AlaMet: 2.976 ± 0.064
2.918AlaAsn: 2.918 ± 0.074
5.156AlaPro: 5.156 ± 0.09
5.117AlaGln: 5.117 ± 0.099
7.802AlaArg: 7.802 ± 0.116
5.458AlaSer: 5.458 ± 0.103
5.553AlaThr: 5.553 ± 0.093
7.733AlaVal: 7.733 ± 0.113
1.554AlaTrp: 1.554 ± 0.047
2.478AlaTyr: 2.478 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.929CysAla: 0.929 ± 0.04
0.109CysCys: 0.109 ± 0.013
0.521CysAsp: 0.521 ± 0.026
0.381CysGlu: 0.381 ± 0.025
0.253CysPhe: 0.253 ± 0.019
0.827CysGly: 0.827 ± 0.037
0.275CysHis: 0.275 ± 0.02
0.362CysIle: 0.362 ± 0.023
0.157CysLys: 0.157 ± 0.013
0.738CysLeu: 0.738 ± 0.03
0.144CysMet: 0.144 ± 0.012
0.227CysAsn: 0.227 ± 0.016
0.519CysPro: 0.519 ± 0.026
0.285CysGln: 0.285 ± 0.021
0.57CysArg: 0.57 ± 0.026
0.424CysSer: 0.424 ± 0.026
0.423CysThr: 0.423 ± 0.025
0.587CysVal: 0.587 ± 0.03
0.103CysTrp: 0.103 ± 0.01
0.19CysTyr: 0.19 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
6.485AspAla: 6.485 ± 0.101
0.479AspCys: 0.479 ± 0.025
3.181AspAsp: 3.181 ± 0.069
3.56AspGlu: 3.56 ± 0.078
2.285AspPhe: 2.285 ± 0.05
4.26AspGly: 4.26 ± 0.088
1.525AspHis: 1.525 ± 0.046
3.053AspIle: 3.053 ± 0.064
1.661AspLys: 1.661 ± 0.053
6.262AspLeu: 6.262 ± 0.094
1.405AspMet: 1.405 ± 0.046
1.44AspAsn: 1.44 ± 0.054
3.42AspPro: 3.42 ± 0.073
2.468AspGln: 2.468 ± 0.059
4.309AspArg: 4.309 ± 0.082
2.666AspSer: 2.666 ± 0.05
3.0AspThr: 3.0 ± 0.059
3.886AspVal: 3.886 ± 0.074
1.13AspTrp: 1.13 ± 0.04
1.604AspTyr: 1.604 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
6.088GluAla: 6.088 ± 0.102
0.379GluCys: 0.379 ± 0.021
2.269GluAsp: 2.269 ± 0.05
2.644GluGlu: 2.644 ± 0.071
1.768GluPhe: 1.768 ± 0.047
3.505GluGly: 3.505 ± 0.067
1.394GluHis: 1.394 ± 0.043
3.394GluIle: 3.394 ± 0.077
1.957GluLys: 1.957 ± 0.058
5.303GluLeu: 5.303 ± 0.097
1.377GluMet: 1.377 ± 0.037
1.646GluAsn: 1.646 ± 0.046
2.691GluPro: 2.691 ± 0.056
3.015GluGln: 3.015 ± 0.07
4.367GluArg: 4.367 ± 0.093
3.148GluSer: 3.148 ± 0.074
3.318GluThr: 3.318 ± 0.071
3.463GluVal: 3.463 ± 0.077
0.627GluTrp: 0.627 ± 0.027
1.128GluTyr: 1.128 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.745PheAla: 3.745 ± 0.074
0.332PheCys: 0.332 ± 0.023
2.623PheAsp: 2.623 ± 0.06
1.907PheGlu: 1.907 ± 0.047
1.408PhePhe: 1.408 ± 0.044
3.373PheGly: 3.373 ± 0.072
0.835PheHis: 0.835 ± 0.034
1.808PheIle: 1.808 ± 0.049
1.077PheLys: 1.077 ± 0.039
3.483PheLeu: 3.483 ± 0.076
0.916PheMet: 0.916 ± 0.037
1.212PheAsn: 1.212 ± 0.037
1.491PhePro: 1.491 ± 0.039
1.111PheGln: 1.111 ± 0.035
1.972PheArg: 1.972 ± 0.045
2.349PheSer: 2.349 ± 0.046
2.064PheThr: 2.064 ± 0.044
2.732PheVal: 2.732 ± 0.06
0.624PheTrp: 0.624 ± 0.03
1.015PheTyr: 1.015 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
7.443GlyAla: 7.443 ± 0.124
0.769GlyCys: 0.769 ± 0.032
4.264GlyAsp: 4.264 ± 0.089
3.937GlyGlu: 3.937 ± 0.077
3.415GlyPhe: 3.415 ± 0.062
6.028GlyGly: 6.028 ± 0.116
2.073GlyHis: 2.073 ± 0.054
4.72GlyIle: 4.72 ± 0.078
3.177GlyLys: 3.177 ± 0.069
8.498GlyLeu: 8.498 ± 0.11
2.401GlyMet: 2.401 ± 0.056
2.357GlyAsn: 2.357 ± 0.066
2.574GlyPro: 2.574 ± 0.06
3.508GlyGln: 3.508 ± 0.083
4.982GlyArg: 4.982 ± 0.084
4.279GlySer: 4.279 ± 0.092
4.249GlyThr: 4.249 ± 0.102
5.954GlyVal: 5.954 ± 0.097
1.336GlyTrp: 1.336 ± 0.04
2.391GlyTyr: 2.391 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
2.593HisAla: 2.593 ± 0.058
0.247HisCys: 0.247 ± 0.021
1.458HisAsp: 1.458 ± 0.043
1.326HisGlu: 1.326 ± 0.046
0.959HisPhe: 0.959 ± 0.038
2.102HisGly: 2.102 ± 0.054
0.667HisHis: 0.667 ± 0.031
1.184HisIle: 1.184 ± 0.039
0.57HisLys: 0.57 ± 0.024
2.624HisLeu: 2.624 ± 0.054
0.534HisMet: 0.534 ± 0.024
0.637HisAsn: 0.637 ± 0.029
1.819HisPro: 1.819 ± 0.049
1.06HisGln: 1.06 ± 0.041
1.736HisArg: 1.736 ± 0.047
1.034HisSer: 1.034 ± 0.034
1.152HisThr: 1.152 ± 0.034
1.548HisVal: 1.548 ± 0.044
0.478HisTrp: 0.478 ± 0.025
0.711HisTyr: 0.711 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.332IleAla: 6.332 ± 0.101
0.43IleCys: 0.43 ± 0.022
4.136IleAsp: 4.136 ± 0.082
4.016IleGlu: 4.016 ± 0.081
1.654IlePhe: 1.654 ± 0.047
4.932IleGly: 4.932 ± 0.087
1.365IleHis: 1.365 ± 0.042
2.307IleIle: 2.307 ± 0.059
1.697IleLys: 1.697 ± 0.046
4.921IleLeu: 4.921 ± 0.097
1.073IleMet: 1.073 ± 0.041
1.991IleAsn: 1.991 ± 0.057
2.801IlePro: 2.801 ± 0.058
1.966IleGln: 1.966 ± 0.053
3.638IleArg: 3.638 ± 0.063
2.948IleSer: 2.948 ± 0.061
3.046IleThr: 3.046 ± 0.067
4.013IleVal: 4.013 ± 0.077
0.598IleTrp: 0.598 ± 0.029
1.199IleTyr: 1.199 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.48LysAla: 3.48 ± 0.082
0.13LysCys: 0.13 ± 0.014
1.659LysAsp: 1.659 ± 0.053
1.449LysGlu: 1.449 ± 0.049
0.889LysPhe: 0.889 ± 0.035
2.156LysGly: 2.156 ± 0.058
0.74LysHis: 0.74 ± 0.03
1.865LysIle: 1.865 ± 0.053
1.351LysLys: 1.351 ± 0.052
3.058LysLeu: 3.058 ± 0.07
0.815LysMet: 0.815 ± 0.034
1.121LysAsn: 1.121 ± 0.045
2.105LysPro: 2.105 ± 0.069
1.417LysGln: 1.417 ± 0.051
2.181LysArg: 2.181 ± 0.063
1.893LysSer: 1.893 ± 0.055
2.093LysThr: 2.093 ± 0.055
2.004LysVal: 2.004 ± 0.053
0.307LysTrp: 0.307 ± 0.02
0.751LysTyr: 0.751 ± 0.035
0.0LysXaa: 0.0 ± 0.0
Leu
12.926LeuAla: 12.926 ± 0.138
0.832LeuCys: 0.832 ± 0.036
6.482LeuAsp: 6.482 ± 0.113
4.861LeuGlu: 4.861 ± 0.092
3.83LeuPhe: 3.83 ± 0.082
8.268LeuGly: 8.268 ± 0.11
2.33LeuHis: 2.33 ± 0.059
6.303LeuIle: 6.303 ± 0.111
3.842LeuLys: 3.842 ± 0.068
10.862LeuLeu: 10.862 ± 0.165
2.685LeuMet: 2.685 ± 0.065
3.48LeuAsn: 3.48 ± 0.064
6.082LeuPro: 6.082 ± 0.093
3.552LeuGln: 3.552 ± 0.074
6.714LeuArg: 6.714 ± 0.1
6.996LeuSer: 6.996 ± 0.089
6.287LeuThr: 6.287 ± 0.096
7.199LeuVal: 7.199 ± 0.101
1.341LeuTrp: 1.341 ± 0.052
2.2LeuTyr: 2.2 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
3.02MetAla: 3.02 ± 0.061
0.115MetCys: 0.115 ± 0.012
1.312MetAsp: 1.312 ± 0.047
0.965MetGlu: 0.965 ± 0.038
0.625MetPhe: 0.625 ± 0.025
1.815MetGly: 1.815 ± 0.053
0.572MetHis: 0.572 ± 0.024
1.443MetIle: 1.443 ± 0.045
0.961MetLys: 0.961 ± 0.03
2.449MetLeu: 2.449 ± 0.059
0.645MetMet: 0.645 ± 0.034
1.093MetAsn: 1.093 ± 0.038
1.45MetPro: 1.45 ± 0.036
1.039MetGln: 1.039 ± 0.036
1.529MetArg: 1.529 ± 0.043
1.728MetSer: 1.728 ± 0.045
1.823MetThr: 1.823 ± 0.049
1.677MetVal: 1.677 ± 0.039
0.188MetTrp: 0.188 ± 0.014
0.4MetTyr: 0.4 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.288AsnAla: 3.288 ± 0.073
0.24AsnCys: 0.24 ± 0.017
1.614AsnAsp: 1.614 ± 0.05
1.45AsnGlu: 1.45 ± 0.039
0.992AsnPhe: 0.992 ± 0.032
2.368AsnGly: 2.368 ± 0.064
0.717AsnHis: 0.717 ± 0.028
1.449AsnIle: 1.449 ± 0.049
0.869AsnLys: 0.869 ± 0.037
3.294AsnLeu: 3.294 ± 0.07
0.631AsnMet: 0.631 ± 0.027
0.925AsnAsn: 0.925 ± 0.039
2.311AsnPro: 2.311 ± 0.055
1.392AsnGln: 1.392 ± 0.045
2.271AsnArg: 2.271 ± 0.056
1.331AsnSer: 1.331 ± 0.051
1.638AsnThr: 1.638 ± 0.052
1.875AsnVal: 1.875 ± 0.052
0.45AsnTrp: 0.45 ± 0.024
0.824AsnTyr: 0.824 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
5.74ProAla: 5.74 ± 0.089
0.335ProCys: 0.335 ± 0.023
3.89ProAsp: 3.89 ± 0.077
3.88ProGlu: 3.88 ± 0.073
1.85ProPhe: 1.85 ± 0.055
4.205ProGly: 4.205 ± 0.079
1.089ProHis: 1.089 ± 0.04
2.853ProIle: 2.853 ± 0.06
1.76ProLys: 1.76 ± 0.05
5.118ProLeu: 5.118 ± 0.087
1.33ProMet: 1.33 ± 0.04
1.604ProAsn: 1.604 ± 0.049
2.631ProPro: 2.631 ± 0.088
1.987ProGln: 1.987 ± 0.053
2.607ProArg: 2.607 ± 0.056
2.693ProSer: 2.693 ± 0.069
2.975ProThr: 2.975 ± 0.076
4.414ProVal: 4.414 ± 0.079
0.732ProTrp: 0.732 ± 0.029
1.2ProTyr: 1.2 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
5.277GlnAla: 5.277 ± 0.094
0.282GlnCys: 0.282 ± 0.02
1.814GlnAsp: 1.814 ± 0.049
1.72GlnGlu: 1.72 ± 0.054
1.45GlnPhe: 1.45 ± 0.044
3.249GlnGly: 3.249 ± 0.079
1.008GlnHis: 1.008 ± 0.039
2.597GlnIle: 2.597 ± 0.062
1.355GlnLys: 1.355 ± 0.046
4.188GlnLeu: 4.188 ± 0.084
1.109GlnMet: 1.109 ± 0.035
1.196GlnAsn: 1.196 ± 0.045
2.358GlnPro: 2.358 ± 0.06
2.006GlnGln: 2.006 ± 0.07
2.851GlnArg: 2.851 ± 0.063
2.544GlnSer: 2.544 ± 0.059
2.481GlnThr: 2.481 ± 0.059
2.919GlnVal: 2.919 ± 0.061
0.624GlnTrp: 0.624 ± 0.029
0.932GlnTyr: 0.932 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
7.002ArgAla: 7.002 ± 0.114
0.522ArgCys: 0.522 ± 0.024
3.778ArgAsp: 3.778 ± 0.076
3.527ArgGlu: 3.527 ± 0.077
2.826ArgPhe: 2.826 ± 0.06
4.099ArgGly: 4.099 ± 0.076
2.049ArgHis: 2.049 ± 0.052
4.241ArgIle: 4.241 ± 0.087
1.899ArgLys: 1.899 ± 0.048
7.954ArgLeu: 7.954 ± 0.123
1.8ArgMet: 1.8 ± 0.05
1.961ArgAsn: 1.961 ± 0.048
3.279ArgPro: 3.279 ± 0.064
3.238ArgGln: 3.238 ± 0.068
4.7ArgArg: 4.7 ± 0.096
3.196ArgSer: 3.196 ± 0.062
3.139ArgThr: 3.139 ± 0.071
4.688ArgVal: 4.688 ± 0.078
1.076ArgTrp: 1.076 ± 0.039
1.937ArgTyr: 1.937 ± 0.055
0.0ArgXaa: 0.0 ± 0.0
Ser
6.203SerAla: 6.203 ± 0.095
0.442SerCys: 0.442 ± 0.027
2.958SerAsp: 2.958 ± 0.06
2.851SerGlu: 2.851 ± 0.059
1.953SerPhe: 1.953 ± 0.055
5.244SerGly: 5.244 ± 0.095
1.155SerHis: 1.155 ± 0.034
2.864SerIle: 2.864 ± 0.062
1.45SerLys: 1.45 ± 0.046
5.912SerLeu: 5.912 ± 0.092
1.355SerMet: 1.355 ± 0.042
1.484SerAsn: 1.484 ± 0.045
2.884SerPro: 2.884 ± 0.061
1.966SerGln: 1.966 ± 0.053
3.399SerArg: 3.399 ± 0.07
3.015SerSer: 3.015 ± 0.08
3.135SerThr: 3.135 ± 0.076
4.143SerVal: 4.143 ± 0.081
0.676SerTrp: 0.676 ± 0.028
1.281SerTyr: 1.281 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
6.155ThrAla: 6.155 ± 0.109
0.393ThrCys: 0.393 ± 0.022
3.316ThrAsp: 3.316 ± 0.07
3.001ThrGlu: 3.001 ± 0.066
1.866ThrPhe: 1.866 ± 0.053
5.044ThrGly: 5.044 ± 0.094
1.336ThrHis: 1.336 ± 0.034
2.718ThrIle: 2.718 ± 0.059
1.362ThrLys: 1.362 ± 0.042
6.667ThrLeu: 6.667 ± 0.093
1.039ThrMet: 1.039 ± 0.037
1.438ThrAsn: 1.438 ± 0.048
3.738ThrPro: 3.738 ± 0.089
2.263ThrGln: 2.263 ± 0.057
3.434ThrArg: 3.434 ± 0.064
2.515ThrSer: 2.515 ± 0.065
3.153ThrThr: 3.153 ± 0.073
4.215ThrVal: 4.215 ± 0.077
0.695ThrTrp: 0.695 ± 0.032
1.316ThrTyr: 1.316 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
7.88ValAla: 7.88 ± 0.111
0.627ValCys: 0.627 ± 0.029
4.404ValAsp: 4.404 ± 0.088
3.464ValGlu: 3.464 ± 0.079
2.66ValPhe: 2.66 ± 0.053
5.203ValGly: 5.203 ± 0.089
1.596ValHis: 1.596 ± 0.042
4.27ValIle: 4.27 ± 0.071
2.05ValLys: 2.05 ± 0.053
7.789ValLeu: 7.789 ± 0.115
1.825ValMet: 1.825 ± 0.054
2.181ValAsn: 2.181 ± 0.049
3.557ValPro: 3.557 ± 0.07
2.635ValGln: 2.635 ± 0.067
4.649ValArg: 4.649 ± 0.088
4.176ValSer: 4.176 ± 0.075
4.282ValThr: 4.282 ± 0.085
5.657ValVal: 5.657 ± 0.097
0.909ValTrp: 0.909 ± 0.037
1.584ValTyr: 1.584 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
1.24TrpAla: 1.24 ± 0.038
0.132TrpCys: 0.132 ± 0.012
0.557TrpAsp: 0.557 ± 0.029
0.416TrpGlu: 0.416 ± 0.024
0.607TrpPhe: 0.607 ± 0.029
0.901TrpGly: 0.901 ± 0.033
0.43TrpHis: 0.43 ± 0.025
0.739TrpIle: 0.739 ± 0.03
0.329TrpLys: 0.329 ± 0.018
2.224TrpLeu: 2.224 ± 0.051
0.372TrpMet: 0.372 ± 0.024
0.342TrpAsn: 0.342 ± 0.022
0.717TrpPro: 0.717 ± 0.033
0.831TrpGln: 0.831 ± 0.038
1.2TrpArg: 1.2 ± 0.044
0.782TrpSer: 0.782 ± 0.027
0.622TrpThr: 0.622 ± 0.031
1.095TrpVal: 1.095 ± 0.036
0.217TrpTrp: 0.217 ± 0.018
0.346TrpTyr: 0.346 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.53TyrAla: 2.53 ± 0.051
0.244TyrCys: 0.244 ± 0.019
1.411TyrAsp: 1.411 ± 0.059
1.193TyrGlu: 1.193 ± 0.038
1.024TyrPhe: 1.024 ± 0.037
2.137TyrGly: 2.137 ± 0.054
0.592TyrHis: 0.592 ± 0.027
1.045TyrIle: 1.045 ± 0.033
0.585TyrLys: 0.585 ± 0.029
2.768TyrLeu: 2.768 ± 0.055
0.417TyrMet: 0.417 ± 0.024
0.677TyrAsn: 0.677 ± 0.032
1.31TyrPro: 1.31 ± 0.041
1.102TyrGln: 1.102 ± 0.042
1.999TyrArg: 1.999 ± 0.052
1.194TyrSer: 1.194 ± 0.038
1.231TyrThr: 1.231 ± 0.047
1.596TyrVal: 1.596 ± 0.044
0.433TyrTrp: 0.433 ± 0.024
0.683TyrTyr: 0.683 ± 0.032
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2521 proteins (841465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski