Amino acid dipepetide frequency for Prevotella sp. S7-1-8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.136AlaAla: 6.136 ± 0.126
1.033AlaCys: 1.033 ± 0.042
4.658AlaAsp: 4.658 ± 0.078
4.539AlaGlu: 4.539 ± 0.089
3.261AlaPhe: 3.261 ± 0.075
4.899AlaGly: 4.899 ± 0.109
1.553AlaHis: 1.553 ± 0.043
5.262AlaIle: 5.262 ± 0.084
5.181AlaLys: 5.181 ± 0.098
7.253AlaLeu: 7.253 ± 0.123
2.518AlaMet: 2.518 ± 0.058
3.951AlaAsn: 3.951 ± 0.084
2.444AlaPro: 2.444 ± 0.066
3.14AlaGln: 3.14 ± 0.072
4.131AlaArg: 4.131 ± 0.084
4.114AlaSer: 4.114 ± 0.076
4.438AlaThr: 4.438 ± 0.083
4.981AlaVal: 4.981 ± 0.083
0.92AlaTrp: 0.92 ± 0.036
3.011AlaTyr: 3.011 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
1.043CysAla: 1.043 ± 0.037
0.204CysCys: 0.204 ± 0.016
0.833CysAsp: 0.833 ± 0.038
0.749CysGlu: 0.749 ± 0.032
0.611CysPhe: 0.611 ± 0.032
1.079CysGly: 1.079 ± 0.041
0.435CysHis: 0.435 ± 0.023
0.691CysIle: 0.691 ± 0.032
0.711CysLys: 0.711 ± 0.034
1.129CysLeu: 1.129 ± 0.039
0.316CysMet: 0.316 ± 0.02
0.593CysAsn: 0.593 ± 0.026
0.579CysPro: 0.579 ± 0.032
0.455CysGln: 0.455 ± 0.026
0.658CysArg: 0.658 ± 0.028
0.738CysSer: 0.738 ± 0.031
0.601CysThr: 0.601 ± 0.028
0.936CysVal: 0.936 ± 0.038
0.177CysTrp: 0.177 ± 0.015
0.54CysTyr: 0.54 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.413AspAla: 4.413 ± 0.082
0.682AspCys: 0.682 ± 0.033
3.281AspAsp: 3.281 ± 0.079
3.979AspGlu: 3.979 ± 0.083
2.994AspPhe: 2.994 ± 0.072
4.622AspGly: 4.622 ± 0.09
1.174AspHis: 1.174 ± 0.041
4.346AspIle: 4.346 ± 0.077
4.32AspLys: 4.32 ± 0.079
4.474AspLeu: 4.474 ± 0.068
1.907AspMet: 1.907 ± 0.056
3.203AspAsn: 3.203 ± 0.071
1.738AspPro: 1.738 ± 0.045
1.425AspGln: 1.425 ± 0.046
2.848AspArg: 2.848 ± 0.074
2.97AspSer: 2.97 ± 0.074
3.207AspThr: 3.207 ± 0.064
3.75AspVal: 3.75 ± 0.074
0.757AspTrp: 0.757 ± 0.031
2.802AspTyr: 2.802 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
4.56GluAla: 4.56 ± 0.097
0.676GluCys: 0.676 ± 0.034
3.142GluAsp: 3.142 ± 0.065
4.047GluGlu: 4.047 ± 0.079
2.074GluPhe: 2.074 ± 0.051
3.636GluGly: 3.636 ± 0.073
1.2GluHis: 1.2 ± 0.044
3.802GluIle: 3.802 ± 0.083
4.46GluLys: 4.46 ± 0.087
5.26GluLeu: 5.26 ± 0.095
1.924GluMet: 1.924 ± 0.06
3.064GluAsn: 3.064 ± 0.069
1.791GluPro: 1.791 ± 0.055
2.445GluGln: 2.445 ± 0.058
4.208GluArg: 4.208 ± 0.083
2.819GluSer: 2.819 ± 0.058
3.19GluThr: 3.19 ± 0.067
3.814GluVal: 3.814 ± 0.084
0.774GluTrp: 0.774 ± 0.028
2.291GluTyr: 2.291 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.232PheAla: 3.232 ± 0.066
0.739PheCys: 0.739 ± 0.03
2.893PheAsp: 2.893 ± 0.059
2.285PheGlu: 2.285 ± 0.059
2.178PhePhe: 2.178 ± 0.068
3.205PheGly: 3.205 ± 0.074
0.912PheHis: 0.912 ± 0.037
2.53PheIle: 2.53 ± 0.068
2.59PheLys: 2.59 ± 0.056
3.637PheLeu: 3.637 ± 0.077
1.303PheMet: 1.303 ± 0.044
2.239PheAsn: 2.239 ± 0.066
1.501PhePro: 1.501 ± 0.047
1.185PheGln: 1.185 ± 0.037
2.064PheArg: 2.064 ± 0.064
2.961PheSer: 2.961 ± 0.065
2.437PheThr: 2.437 ± 0.074
3.351PheVal: 3.351 ± 0.079
0.512PheTrp: 0.512 ± 0.029
1.714PheTyr: 1.714 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.761GlyAla: 4.761 ± 0.103
0.96GlyCys: 0.96 ± 0.039
3.755GlyAsp: 3.755 ± 0.078
3.821GlyGlu: 3.821 ± 0.066
3.032GlyPhe: 3.032 ± 0.068
4.753GlyGly: 4.753 ± 0.11
1.529GlyHis: 1.529 ± 0.049
4.666GlyIle: 4.666 ± 0.078
5.465GlyLys: 5.465 ± 0.093
5.8GlyLeu: 5.8 ± 0.1
2.287GlyMet: 2.287 ± 0.056
3.381GlyAsn: 3.381 ± 0.068
1.239GlyPro: 1.239 ± 0.047
2.338GlyGln: 2.338 ± 0.062
3.643GlyArg: 3.643 ± 0.075
3.849GlySer: 3.849 ± 0.08
3.936GlyThr: 3.936 ± 0.074
5.078GlyVal: 5.078 ± 0.088
0.875GlyTrp: 0.875 ± 0.041
3.131GlyTyr: 3.131 ± 0.073
0.0GlyXaa: 0.0 ± 0.0
His
1.679HisAla: 1.679 ± 0.05
0.34HisCys: 0.34 ± 0.022
1.244HisAsp: 1.244 ± 0.039
1.109HisGlu: 1.109 ± 0.04
1.042HisPhe: 1.042 ± 0.041
1.572HisGly: 1.572 ± 0.046
0.607HisHis: 0.607 ± 0.029
1.381HisIle: 1.381 ± 0.04
1.137HisLys: 1.137 ± 0.036
1.852HisLeu: 1.852 ± 0.05
0.384HisMet: 0.384 ± 0.021
0.991HisAsn: 0.991 ± 0.034
1.011HisPro: 1.011 ± 0.039
0.704HisGln: 0.704 ± 0.032
1.14HisArg: 1.14 ± 0.041
1.097HisSer: 1.097 ± 0.043
1.033HisThr: 1.033 ± 0.037
1.42HisVal: 1.42 ± 0.044
0.297HisTrp: 0.297 ± 0.021
0.897HisTyr: 0.897 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
5.157IleAla: 5.157 ± 0.107
0.924IleCys: 0.924 ± 0.032
4.508IleAsp: 4.508 ± 0.083
3.874IleGlu: 3.874 ± 0.078
2.595IlePhe: 2.595 ± 0.06
4.466IleGly: 4.466 ± 0.081
1.274IleHis: 1.274 ± 0.048
3.987IleIle: 3.987 ± 0.091
4.148IleLys: 4.148 ± 0.083
4.849IleLeu: 4.849 ± 0.096
1.548IleMet: 1.548 ± 0.045
3.238IleAsn: 3.238 ± 0.069
2.791IlePro: 2.791 ± 0.068
1.9IleGln: 1.9 ± 0.047
3.11IleArg: 3.11 ± 0.073
3.967IleSer: 3.967 ± 0.089
3.577IleThr: 3.577 ± 0.084
4.581IleVal: 4.581 ± 0.086
0.566IleTrp: 0.566 ± 0.029
2.452IleTyr: 2.452 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
5.301LysAla: 5.301 ± 0.103
0.62LysCys: 0.62 ± 0.026
3.925LysAsp: 3.925 ± 0.073
4.607LysGlu: 4.607 ± 0.097
2.167LysPhe: 2.167 ± 0.057
4.399LysGly: 4.399 ± 0.079
1.324LysHis: 1.324 ± 0.043
4.248LysIle: 4.248 ± 0.079
4.999LysLys: 4.999 ± 0.101
5.397LysLeu: 5.397 ± 0.096
2.167LysMet: 2.167 ± 0.048
3.51LysAsn: 3.51 ± 0.08
2.479LysPro: 2.479 ± 0.059
2.629LysGln: 2.629 ± 0.06
3.897LysArg: 3.897 ± 0.077
3.538LysSer: 3.538 ± 0.069
3.975LysThr: 3.975 ± 0.075
4.162LysVal: 4.162 ± 0.067
0.861LysTrp: 0.861 ± 0.033
2.871LysTyr: 2.871 ± 0.063
0.0LysXaa: 0.0 ± 0.0
Leu
6.888LeuAla: 6.888 ± 0.108
1.399LeuCys: 1.399 ± 0.045
4.831LeuAsp: 4.831 ± 0.083
4.417LeuGlu: 4.417 ± 0.092
4.004LeuPhe: 4.004 ± 0.075
5.716LeuGly: 5.716 ± 0.106
1.758LeuHis: 1.758 ± 0.054
4.659LeuIle: 4.659 ± 0.089
5.728LeuLys: 5.728 ± 0.087
7.888LeuLeu: 7.888 ± 0.145
2.632LeuMet: 2.632 ± 0.059
4.245LeuAsn: 4.245 ± 0.085
3.759LeuPro: 3.759 ± 0.077
3.055LeuGln: 3.055 ± 0.059
5.412LeuArg: 5.412 ± 0.095
6.365LeuSer: 6.365 ± 0.108
4.928LeuThr: 4.928 ± 0.087
5.321LeuVal: 5.321 ± 0.098
0.954LeuTrp: 0.954 ± 0.038
3.319LeuTyr: 3.319 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.842MetAla: 2.842 ± 0.065
0.292MetCys: 0.292 ± 0.02
1.728MetAsp: 1.728 ± 0.042
1.87MetGlu: 1.87 ± 0.047
1.102MetPhe: 1.102 ± 0.041
2.223MetGly: 2.223 ± 0.059
0.446MetHis: 0.446 ± 0.025
1.453MetIle: 1.453 ± 0.047
2.251MetLys: 2.251 ± 0.055
2.636MetLeu: 2.636 ± 0.065
0.999MetMet: 0.999 ± 0.039
1.429MetAsn: 1.429 ± 0.044
1.424MetPro: 1.424 ± 0.045
1.138MetGln: 1.138 ± 0.044
1.945MetArg: 1.945 ± 0.053
1.769MetSer: 1.769 ± 0.048
1.773MetThr: 1.773 ± 0.05
1.935MetVal: 1.935 ± 0.056
0.22MetTrp: 0.22 ± 0.017
0.837MetTyr: 0.837 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.98AsnAla: 3.98 ± 0.072
0.508AsnCys: 0.508 ± 0.028
2.889AsnAsp: 2.889 ± 0.066
2.85AsnGlu: 2.85 ± 0.061
2.041AsnPhe: 2.041 ± 0.058
3.897AsnGly: 3.897 ± 0.081
1.074AsnHis: 1.074 ± 0.037
3.679AsnIle: 3.679 ± 0.07
3.257AsnLys: 3.257 ± 0.067
4.113AsnLeu: 4.113 ± 0.084
1.426AsnMet: 1.426 ± 0.046
2.601AsnAsn: 2.601 ± 0.068
2.287AsnPro: 2.287 ± 0.051
1.452AsnGln: 1.452 ± 0.043
2.468AsnArg: 2.468 ± 0.062
2.542AsnSer: 2.542 ± 0.072
2.644AsnThr: 2.644 ± 0.062
3.546AsnVal: 3.546 ± 0.073
0.592AsnTrp: 0.592 ± 0.03
2.265AsnTyr: 2.265 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
2.61ProAla: 2.61 ± 0.06
0.425ProCys: 0.425 ± 0.025
2.448ProAsp: 2.448 ± 0.058
2.607ProGlu: 2.607 ± 0.06
1.796ProPhe: 1.796 ± 0.052
2.07ProGly: 2.07 ± 0.052
0.783ProHis: 0.783 ± 0.035
2.352ProIle: 2.352 ± 0.062
2.326ProLys: 2.326 ± 0.051
3.09ProLeu: 3.09 ± 0.064
1.138ProMet: 1.138 ± 0.037
1.981ProAsn: 1.981 ± 0.048
0.848ProPro: 0.848 ± 0.037
1.424ProGln: 1.424 ± 0.045
1.661ProArg: 1.661 ± 0.052
2.147ProSer: 2.147 ± 0.06
2.369ProThr: 2.369 ± 0.049
2.427ProVal: 2.427 ± 0.059
0.437ProTrp: 0.437 ± 0.026
1.592ProTyr: 1.592 ± 0.054
0.0ProXaa: 0.0 ± 0.0
Gln
2.838GlnAla: 2.838 ± 0.07
0.374GlnCys: 0.374 ± 0.022
1.758GlnAsp: 1.758 ± 0.051
2.147GlnGlu: 2.147 ± 0.061
1.337GlnPhe: 1.337 ± 0.042
2.212GlnGly: 2.212 ± 0.061
0.641GlnHis: 0.641 ± 0.031
2.273GlnIle: 2.273 ± 0.056
2.471GlnLys: 2.471 ± 0.061
3.414GlnLeu: 3.414 ± 0.068
1.136GlnMet: 1.136 ± 0.041
1.737GlnAsn: 1.737 ± 0.059
1.308GlnPro: 1.308 ± 0.042
1.531GlnGln: 1.531 ± 0.051
2.128GlnArg: 2.128 ± 0.06
1.942GlnSer: 1.942 ± 0.056
2.022GlnThr: 2.022 ± 0.042
2.155GlnVal: 2.155 ± 0.059
0.482GlnTrp: 0.482 ± 0.026
1.416GlnTyr: 1.416 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
3.919ArgAla: 3.919 ± 0.085
0.62ArgCys: 0.62 ± 0.028
2.968ArgAsp: 2.968 ± 0.069
3.363ArgGlu: 3.363 ± 0.066
2.403ArgPhe: 2.403 ± 0.055
3.209ArgGly: 3.209 ± 0.07
1.544ArgHis: 1.544 ± 0.054
3.158ArgIle: 3.158 ± 0.062
3.806ArgLys: 3.806 ± 0.088
5.276ArgLeu: 5.276 ± 0.102
1.892ArgMet: 1.892 ± 0.052
2.508ArgAsn: 2.508 ± 0.056
1.9ArgPro: 1.9 ± 0.054
2.646ArgGln: 2.646 ± 0.071
3.305ArgArg: 3.305 ± 0.077
2.637ArgSer: 2.637 ± 0.071
2.563ArgThr: 2.563 ± 0.061
3.529ArgVal: 3.529 ± 0.07
0.762ArgTrp: 0.762 ± 0.037
2.471ArgTyr: 2.471 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
4.434SerAla: 4.434 ± 0.081
0.846SerCys: 0.846 ± 0.032
3.136SerAsp: 3.136 ± 0.066
3.102SerGlu: 3.102 ± 0.079
2.94SerPhe: 2.94 ± 0.067
4.002SerGly: 4.002 ± 0.082
1.235SerHis: 1.235 ± 0.043
3.665SerIle: 3.665 ± 0.076
3.447SerLys: 3.447 ± 0.07
5.5SerLeu: 5.5 ± 0.108
1.745SerMet: 1.745 ± 0.053
2.528SerAsn: 2.528 ± 0.075
2.147SerPro: 2.147 ± 0.06
2.016SerGln: 2.016 ± 0.053
2.832SerArg: 2.832 ± 0.06
3.269SerSer: 3.269 ± 0.082
2.957SerThr: 2.957 ± 0.064
4.39SerVal: 4.39 ± 0.068
0.723SerTrp: 0.723 ± 0.039
2.486SerTyr: 2.486 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
4.307ThrAla: 4.307 ± 0.077
0.682ThrCys: 0.682 ± 0.03
3.361ThrAsp: 3.361 ± 0.071
2.932ThrGlu: 2.932 ± 0.068
2.609ThrPhe: 2.609 ± 0.064
3.84ThrGly: 3.84 ± 0.075
1.116ThrHis: 1.116 ± 0.043
3.813ThrIle: 3.813 ± 0.082
3.092ThrLys: 3.092 ± 0.067
5.72ThrLeu: 5.72 ± 0.093
1.44ThrMet: 1.44 ± 0.039
2.582ThrAsn: 2.582 ± 0.065
2.776ThrPro: 2.776 ± 0.069
1.799ThrGln: 1.799 ± 0.049
2.491ThrArg: 2.491 ± 0.064
3.15ThrSer: 3.15 ± 0.074
3.276ThrThr: 3.276 ± 0.078
3.829ThrVal: 3.829 ± 0.077
0.688ThrTrp: 0.688 ± 0.027
2.186ThrTyr: 2.186 ± 0.048
0.0ThrXaa: 0.0 ± 0.0
Val
5.409ValAla: 5.409 ± 0.101
1.038ValCys: 1.038 ± 0.034
4.223ValAsp: 4.223 ± 0.08
4.031ValGlu: 4.031 ± 0.077
2.865ValPhe: 2.865 ± 0.064
4.731ValGly: 4.731 ± 0.092
1.039ValHis: 1.039 ± 0.036
4.252ValIle: 4.252 ± 0.08
4.344ValLys: 4.344 ± 0.085
5.235ValLeu: 5.235 ± 0.081
1.945ValMet: 1.945 ± 0.048
3.352ValAsn: 3.352 ± 0.065
2.614ValPro: 2.614 ± 0.065
1.974ValGln: 1.974 ± 0.05
3.605ValArg: 3.605 ± 0.078
4.663ValSer: 4.663 ± 0.076
3.841ValThr: 3.841 ± 0.081
5.16ValVal: 5.16 ± 0.1
0.773ValTrp: 0.773 ± 0.032
2.634ValTyr: 2.634 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.786TrpAla: 0.786 ± 0.036
0.179TrpCys: 0.179 ± 0.014
0.686TrpAsp: 0.686 ± 0.037
0.632TrpGlu: 0.632 ± 0.031
0.491TrpPhe: 0.491 ± 0.026
0.879TrpGly: 0.879 ± 0.035
0.283TrpHis: 0.283 ± 0.02
0.694TrpIle: 0.694 ± 0.03
0.794TrpLys: 0.794 ± 0.036
1.31TrpLeu: 1.31 ± 0.042
0.463TrpMet: 0.463 ± 0.023
0.68TrpAsn: 0.68 ± 0.031
0.317TrpPro: 0.317 ± 0.023
0.608TrpGln: 0.608 ± 0.026
0.676TrpArg: 0.676 ± 0.035
0.612TrpSer: 0.612 ± 0.033
0.584TrpThr: 0.584 ± 0.028
0.692TrpVal: 0.692 ± 0.034
0.2TrpTrp: 0.2 ± 0.02
0.482TrpTyr: 0.482 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.128TyrAla: 3.128 ± 0.067
0.537TyrCys: 0.537 ± 0.026
2.628TyrAsp: 2.628 ± 0.06
2.22TyrGlu: 2.22 ± 0.062
1.926TyrPhe: 1.926 ± 0.051
2.89TyrGly: 2.89 ± 0.059
1.008TyrHis: 1.008 ± 0.037
2.605TyrIle: 2.605 ± 0.065
2.549TyrLys: 2.549 ± 0.064
3.443TyrLeu: 3.443 ± 0.077
1.138TyrMet: 1.138 ± 0.044
2.294TyrAsn: 2.294 ± 0.064
1.576TyrPro: 1.576 ± 0.043
1.456TyrGln: 1.456 ± 0.047
2.259TyrArg: 2.259 ± 0.056
2.251TyrSer: 2.251 ± 0.054
2.334TyrThr: 2.334 ± 0.056
2.672TyrVal: 2.672 ± 0.052
0.481TyrTrp: 0.481 ± 0.026
1.855TyrTyr: 1.855 ± 0.059
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2240 proteins (746712 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski