Amino acid dipepetide frequency for Escherichia phage Lys8385Vzw

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.863AlaAla: 11.863 ± 1.708
1.133AlaCys: 1.133 ± 0.265
6.065AlaAsp: 6.065 ± 0.644
7.997AlaGlu: 7.997 ± 1.025
3.732AlaPhe: 3.732 ± 0.653
9.53AlaGly: 9.53 ± 1.06
1.666AlaHis: 1.666 ± 0.416
4.332AlaIle: 4.332 ± 0.559
3.799AlaLys: 3.799 ± 0.508
8.597AlaLeu: 8.597 ± 0.78
2.866AlaMet: 2.866 ± 0.397
3.332AlaAsn: 3.332 ± 0.528
2.532AlaPro: 2.532 ± 0.468
3.665AlaGln: 3.665 ± 0.457
5.998AlaArg: 5.998 ± 0.881
6.198AlaSer: 6.198 ± 0.737
5.865AlaThr: 5.865 ± 0.762
6.931AlaVal: 6.931 ± 0.691
1.4AlaTrp: 1.4 ± 0.336
2.599AlaTyr: 2.599 ± 0.482
0.0AlaXaa: 0.0 ± 0.0
Cys
0.733CysAla: 0.733 ± 0.255
0.333CysCys: 0.333 ± 0.132
0.733CysAsp: 0.733 ± 0.218
0.666CysGlu: 0.666 ± 0.242
0.333CysPhe: 0.333 ± 0.156
1.4CysGly: 1.4 ± 0.322
0.333CysHis: 0.333 ± 0.147
0.4CysIle: 0.4 ± 0.161
0.533CysLys: 0.533 ± 0.195
0.866CysLeu: 0.866 ± 0.204
0.4CysMet: 0.4 ± 0.156
0.467CysAsn: 0.467 ± 0.174
0.533CysPro: 0.533 ± 0.176
0.733CysGln: 0.733 ± 0.187
1.266CysArg: 1.266 ± 0.294
1.066CysSer: 1.066 ± 0.252
0.933CysThr: 0.933 ± 0.281
1.066CysVal: 1.066 ± 0.347
0.467CysTrp: 0.467 ± 0.202
0.533CysTyr: 0.533 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
5.731AspAla: 5.731 ± 0.658
0.4AspCys: 0.4 ± 0.177
3.399AspAsp: 3.399 ± 0.559
3.599AspGlu: 3.599 ± 0.469
1.933AspPhe: 1.933 ± 0.443
4.798AspGly: 4.798 ± 0.467
0.733AspHis: 0.733 ± 0.229
3.066AspIle: 3.066 ± 0.496
2.266AspLys: 2.266 ± 0.427
4.065AspLeu: 4.065 ± 0.59
1.466AspMet: 1.466 ± 0.347
3.066AspAsn: 3.066 ± 0.432
2.732AspPro: 2.732 ± 0.458
1.733AspGln: 1.733 ± 0.322
2.999AspArg: 2.999 ± 0.461
2.399AspSer: 2.399 ± 0.436
2.999AspThr: 2.999 ± 0.439
4.132AspVal: 4.132 ± 0.532
0.8AspTrp: 0.8 ± 0.217
1.466AspTyr: 1.466 ± 0.277
0.0AspXaa: 0.0 ± 0.0
Glu
7.264GluAla: 7.264 ± 0.846
1.0GluCys: 1.0 ± 0.295
3.199GluAsp: 3.199 ± 0.492
3.799GluGlu: 3.799 ± 0.597
2.333GluPhe: 2.333 ± 0.368
3.865GluGly: 3.865 ± 0.434
1.4GluHis: 1.4 ± 0.336
3.199GluIle: 3.199 ± 0.485
4.399GluLys: 4.399 ± 0.485
6.198GluLeu: 6.198 ± 0.789
2.066GluMet: 2.066 ± 0.354
2.732GluAsn: 2.732 ± 0.462
2.799GluPro: 2.799 ± 0.544
3.466GluGln: 3.466 ± 0.507
3.532GluArg: 3.532 ± 0.485
3.999GluSer: 3.999 ± 0.668
2.932GluThr: 2.932 ± 0.495
3.999GluVal: 3.999 ± 0.561
1.4GluTrp: 1.4 ± 0.316
1.599GluTyr: 1.599 ± 0.317
0.0GluXaa: 0.0 ± 0.0
Phe
2.799PheAla: 2.799 ± 0.442
0.6PheCys: 0.6 ± 0.178
2.066PheAsp: 2.066 ± 0.448
1.999PheGlu: 1.999 ± 0.385
1.0PhePhe: 1.0 ± 0.334
2.399PheGly: 2.399 ± 0.382
0.4PheHis: 0.4 ± 0.204
1.533PheIle: 1.533 ± 0.337
1.2PheLys: 1.2 ± 0.348
2.399PheLeu: 2.399 ± 0.463
1.066PheMet: 1.066 ± 0.298
1.333PheAsn: 1.333 ± 0.293
1.266PhePro: 1.266 ± 0.272
0.8PheGln: 0.8 ± 0.207
2.999PheArg: 2.999 ± 0.467
3.599PheSer: 3.599 ± 0.518
2.066PheThr: 2.066 ± 0.392
2.532PheVal: 2.532 ± 0.369
0.6PheTrp: 0.6 ± 0.179
1.133PheTyr: 1.133 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
6.598GlyAla: 6.598 ± 0.73
0.6GlyCys: 0.6 ± 0.183
4.665GlyAsp: 4.665 ± 0.577
5.332GlyGlu: 5.332 ± 0.705
3.199GlyPhe: 3.199 ± 0.46
5.598GlyGly: 5.598 ± 0.83
1.2GlyHis: 1.2 ± 0.28
4.132GlyIle: 4.132 ± 0.625
5.265GlyLys: 5.265 ± 0.597
4.798GlyLeu: 4.798 ± 0.554
2.599GlyMet: 2.599 ± 0.395
3.732GlyAsn: 3.732 ± 0.506
2.666GlyPro: 2.666 ± 1.265
3.532GlyGln: 3.532 ± 0.603
4.998GlyArg: 4.998 ± 0.621
4.732GlySer: 4.732 ± 0.592
3.932GlyThr: 3.932 ± 0.574
5.398GlyVal: 5.398 ± 0.608
1.266GlyTrp: 1.266 ± 0.3
1.733GlyTyr: 1.733 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
2.066HisAla: 2.066 ± 0.353
0.467HisCys: 0.467 ± 0.189
1.4HisAsp: 1.4 ± 0.321
1.2HisGlu: 1.2 ± 0.306
0.8HisPhe: 0.8 ± 0.311
1.733HisGly: 1.733 ± 0.29
0.866HisHis: 0.866 ± 0.255
1.266HisIle: 1.266 ± 0.302
0.933HisLys: 0.933 ± 0.256
2.199HisLeu: 2.199 ± 0.379
0.4HisMet: 0.4 ± 0.161
0.8HisAsn: 0.8 ± 0.256
1.266HisPro: 1.266 ± 0.289
0.533HisGln: 0.533 ± 0.258
0.8HisArg: 0.8 ± 0.253
1.2HisSer: 1.2 ± 0.312
0.8HisThr: 0.8 ± 0.235
1.066HisVal: 1.066 ± 0.252
0.2HisTrp: 0.2 ± 0.122
0.267HisTyr: 0.267 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
3.532IleAla: 3.532 ± 0.57
0.866IleCys: 0.866 ± 0.251
3.665IleAsp: 3.665 ± 0.451
2.866IleGlu: 2.866 ± 0.523
1.066IlePhe: 1.066 ± 0.274
2.999IleGly: 2.999 ± 0.461
1.133IleHis: 1.133 ± 0.293
2.399IleIle: 2.399 ± 0.402
2.999IleLys: 2.999 ± 0.409
3.799IleLeu: 3.799 ± 0.633
0.933IleMet: 0.933 ± 0.217
2.666IleAsn: 2.666 ± 0.494
2.466IlePro: 2.466 ± 0.366
1.799IleGln: 1.799 ± 0.41
4.265IleArg: 4.265 ± 0.585
4.465IleSer: 4.465 ± 0.558
3.599IleThr: 3.599 ± 0.544
2.666IleVal: 2.666 ± 0.45
0.4IleTrp: 0.4 ± 0.138
0.733IleTyr: 0.733 ± 0.212
0.0IleXaa: 0.0 ± 0.0
Lys
4.998LysAla: 4.998 ± 0.575
0.866LysCys: 0.866 ± 0.246
2.666LysAsp: 2.666 ± 0.464
3.332LysGlu: 3.332 ± 0.508
1.999LysPhe: 1.999 ± 0.435
4.598LysGly: 4.598 ± 0.676
1.266LysHis: 1.266 ± 0.274
2.532LysIle: 2.532 ± 0.413
2.666LysLys: 2.666 ± 0.468
3.865LysLeu: 3.865 ± 0.537
1.333LysMet: 1.333 ± 0.451
1.933LysAsn: 1.933 ± 0.493
2.133LysPro: 2.133 ± 0.35
2.333LysGln: 2.333 ± 0.322
2.799LysArg: 2.799 ± 0.502
3.132LysSer: 3.132 ± 0.557
3.865LysThr: 3.865 ± 0.521
3.199LysVal: 3.199 ± 0.506
0.467LysTrp: 0.467 ± 0.161
1.266LysTyr: 1.266 ± 0.322
0.0LysXaa: 0.0 ± 0.0
Leu
9.264LeuAla: 9.264 ± 0.896
1.533LeuCys: 1.533 ± 0.361
3.932LeuAsp: 3.932 ± 0.55
4.665LeuGlu: 4.665 ± 0.593
2.466LeuPhe: 2.466 ± 0.408
4.465LeuGly: 4.465 ± 0.597
1.799LeuHis: 1.799 ± 0.405
3.799LeuIle: 3.799 ± 0.514
5.198LeuLys: 5.198 ± 0.608
6.265LeuLeu: 6.265 ± 0.604
2.466LeuMet: 2.466 ± 0.381
3.599LeuAsn: 3.599 ± 0.45
3.799LeuPro: 3.799 ± 0.455
3.532LeuGln: 3.532 ± 0.42
6.531LeuArg: 6.531 ± 0.647
6.265LeuSer: 6.265 ± 0.607
5.398LeuThr: 5.398 ± 0.706
4.998LeuVal: 4.998 ± 0.525
1.2LeuTrp: 1.2 ± 0.282
1.933LeuTyr: 1.933 ± 0.411
0.0LeuXaa: 0.0 ± 0.0
Met
3.199MetAla: 3.199 ± 0.346
0.133MetCys: 0.133 ± 0.095
0.933MetAsp: 0.933 ± 0.233
1.4MetGlu: 1.4 ± 0.319
0.4MetPhe: 0.4 ± 0.188
1.4MetGly: 1.4 ± 0.284
0.133MetHis: 0.133 ± 0.103
1.266MetIle: 1.266 ± 0.299
1.533MetLys: 1.533 ± 0.337
2.599MetLeu: 2.599 ± 0.39
0.533MetMet: 0.533 ± 0.175
1.733MetAsn: 1.733 ± 0.305
1.533MetPro: 1.533 ± 0.315
1.266MetGln: 1.266 ± 0.256
1.733MetArg: 1.733 ± 0.33
2.266MetSer: 2.266 ± 0.366
2.466MetThr: 2.466 ± 0.419
1.133MetVal: 1.133 ± 0.282
0.267MetTrp: 0.267 ± 0.126
0.267MetTyr: 0.267 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
5.065AsnAla: 5.065 ± 0.582
0.666AsnCys: 0.666 ± 0.231
2.066AsnAsp: 2.066 ± 0.332
2.666AsnGlu: 2.666 ± 0.508
0.933AsnPhe: 0.933 ± 0.304
4.132AsnGly: 4.132 ± 0.541
1.133AsnHis: 1.133 ± 0.305
2.399AsnIle: 2.399 ± 0.487
1.933AsnLys: 1.933 ± 0.348
3.132AsnLeu: 3.132 ± 0.468
1.0AsnMet: 1.0 ± 0.274
1.999AsnAsn: 1.999 ± 0.39
2.666AsnPro: 2.666 ± 0.368
1.666AsnGln: 1.666 ± 0.33
2.799AsnArg: 2.799 ± 0.479
2.866AsnSer: 2.866 ± 0.558
1.733AsnThr: 1.733 ± 0.274
2.199AsnVal: 2.199 ± 0.361
0.533AsnTrp: 0.533 ± 0.18
1.133AsnTyr: 1.133 ± 0.273
0.0AsnXaa: 0.0 ± 0.0
Pro
4.332ProAla: 4.332 ± 0.472
0.6ProCys: 0.6 ± 0.226
3.066ProAsp: 3.066 ± 0.462
5.132ProGlu: 5.132 ± 0.643
1.4ProPhe: 1.4 ± 0.388
3.132ProGly: 3.132 ± 0.4
1.2ProHis: 1.2 ± 0.281
0.8ProIle: 0.8 ± 0.28
2.266ProLys: 2.266 ± 0.575
2.932ProLeu: 2.932 ± 0.475
0.333ProMet: 0.333 ± 0.161
1.133ProAsn: 1.133 ± 0.336
1.999ProPro: 1.999 ± 0.443
1.666ProGln: 1.666 ± 0.419
2.199ProArg: 2.199 ± 0.406
2.866ProSer: 2.866 ± 0.402
2.066ProThr: 2.066 ± 0.274
4.399ProVal: 4.399 ± 0.596
0.467ProTrp: 0.467 ± 0.17
0.933ProTyr: 0.933 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
4.065GlnAla: 4.065 ± 0.683
0.8GlnCys: 0.8 ± 0.182
1.666GlnAsp: 1.666 ± 0.308
2.199GlnGlu: 2.199 ± 0.431
1.2GlnPhe: 1.2 ± 0.265
3.132GlnGly: 3.132 ± 0.581
1.066GlnHis: 1.066 ± 0.264
2.866GlnIle: 2.866 ± 0.377
3.332GlnLys: 3.332 ± 0.389
3.399GlnLeu: 3.399 ± 0.455
1.133GlnMet: 1.133 ± 0.323
1.599GlnAsn: 1.599 ± 0.485
1.666GlnPro: 1.666 ± 0.335
2.399GlnGln: 2.399 ± 0.542
2.266GlnArg: 2.266 ± 0.513
3.066GlnSer: 3.066 ± 0.51
1.799GlnThr: 1.799 ± 0.394
2.466GlnVal: 2.466 ± 0.38
0.6GlnTrp: 0.6 ± 0.186
1.4GlnTyr: 1.4 ± 0.289
0.0GlnXaa: 0.0 ± 0.0
Arg
5.065ArgAla: 5.065 ± 0.637
1.133ArgCys: 1.133 ± 0.284
3.466ArgAsp: 3.466 ± 0.635
5.065ArgGlu: 5.065 ± 0.653
2.666ArgPhe: 2.666 ± 0.478
4.132ArgGly: 4.132 ± 0.524
1.533ArgHis: 1.533 ± 0.297
3.199ArgIle: 3.199 ± 0.527
3.332ArgLys: 3.332 ± 0.497
6.664ArgLeu: 6.664 ± 0.688
1.866ArgMet: 1.866 ± 0.369
3.266ArgAsn: 3.266 ± 0.471
2.266ArgPro: 2.266 ± 0.349
2.666ArgGln: 2.666 ± 0.441
5.132ArgArg: 5.132 ± 0.668
2.599ArgSer: 2.599 ± 0.45
3.332ArgThr: 3.332 ± 0.46
4.332ArgVal: 4.332 ± 0.622
1.0ArgTrp: 1.0 ± 0.285
2.399ArgTyr: 2.399 ± 0.474
0.0ArgXaa: 0.0 ± 0.0
Ser
8.464SerAla: 8.464 ± 1.052
0.8SerCys: 0.8 ± 0.218
3.332SerAsp: 3.332 ± 0.531
4.065SerGlu: 4.065 ± 0.565
1.799SerPhe: 1.799 ± 0.333
6.798SerGly: 6.798 ± 0.76
1.066SerHis: 1.066 ± 0.266
3.266SerIle: 3.266 ± 0.445
2.199SerLys: 2.199 ± 0.381
5.398SerLeu: 5.398 ± 0.567
2.066SerMet: 2.066 ± 0.427
2.466SerAsn: 2.466 ± 0.42
2.266SerPro: 2.266 ± 0.401
3.399SerGln: 3.399 ± 0.585
3.799SerArg: 3.799 ± 0.512
4.199SerSer: 4.199 ± 0.67
3.199SerThr: 3.199 ± 0.537
5.998SerVal: 5.998 ± 0.835
0.666SerTrp: 0.666 ± 0.236
1.866SerTyr: 1.866 ± 0.368
0.0SerXaa: 0.0 ± 0.0
Thr
5.931ThrAla: 5.931 ± 0.801
0.467ThrCys: 0.467 ± 0.159
2.999ThrAsp: 2.999 ± 0.444
3.665ThrGlu: 3.665 ± 0.646
2.599ThrPhe: 2.599 ± 0.423
5.332ThrGly: 5.332 ± 0.787
1.533ThrHis: 1.533 ± 0.319
2.866ThrIle: 2.866 ± 0.551
1.866ThrLys: 1.866 ± 0.33
5.798ThrLeu: 5.798 ± 0.685
1.066ThrMet: 1.066 ± 0.295
1.933ThrAsn: 1.933 ± 0.321
3.199ThrPro: 3.199 ± 0.483
2.266ThrGln: 2.266 ± 0.297
3.066ThrArg: 3.066 ± 0.446
3.199ThrSer: 3.199 ± 0.642
3.599ThrThr: 3.599 ± 0.593
4.998ThrVal: 4.998 ± 0.602
0.533ThrTrp: 0.533 ± 0.199
1.333ThrTyr: 1.333 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
5.798ValAla: 5.798 ± 0.623
0.866ValCys: 0.866 ± 0.24
2.799ValAsp: 2.799 ± 0.399
3.932ValGlu: 3.932 ± 0.544
2.266ValPhe: 2.266 ± 0.447
3.732ValGly: 3.732 ± 0.521
0.666ValHis: 0.666 ± 0.215
4.199ValIle: 4.199 ± 0.563
3.932ValLys: 3.932 ± 0.594
6.331ValLeu: 6.331 ± 0.845
1.866ValMet: 1.866 ± 0.3
3.865ValAsn: 3.865 ± 0.483
3.532ValPro: 3.532 ± 0.444
2.532ValGln: 2.532 ± 0.442
4.532ValArg: 4.532 ± 0.584
5.798ValSer: 5.798 ± 0.659
5.132ValThr: 5.132 ± 0.666
4.865ValVal: 4.865 ± 0.664
1.0ValTrp: 1.0 ± 0.302
1.666ValTyr: 1.666 ± 0.333
0.0ValXaa: 0.0 ± 0.0
Trp
0.866TrpAla: 0.866 ± 0.276
0.133TrpCys: 0.133 ± 0.085
0.533TrpAsp: 0.533 ± 0.245
0.866TrpGlu: 0.866 ± 0.212
0.533TrpPhe: 0.533 ± 0.207
1.066TrpGly: 1.066 ± 0.273
0.533TrpHis: 0.533 ± 0.21
0.533TrpIle: 0.533 ± 0.184
0.533TrpLys: 0.533 ± 0.199
1.333TrpLeu: 1.333 ± 0.321
0.4TrpMet: 0.4 ± 0.181
0.267TrpAsn: 0.267 ± 0.125
0.6TrpPro: 0.6 ± 0.185
0.8TrpGln: 0.8 ± 0.178
1.466TrpArg: 1.466 ± 0.25
0.733TrpSer: 0.733 ± 0.173
0.733TrpThr: 0.733 ± 0.203
1.333TrpVal: 1.333 ± 0.292
0.4TrpTrp: 0.4 ± 0.178
0.533TrpTyr: 0.533 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.426
0.4TyrCys: 0.4 ± 0.168
1.133TyrAsp: 1.133 ± 0.232
0.733TyrGlu: 0.733 ± 0.219
1.133TyrPhe: 1.133 ± 0.264
1.733TyrGly: 1.733 ± 0.373
0.6TyrHis: 0.6 ± 0.156
1.266TyrIle: 1.266 ± 0.346
1.133TyrLys: 1.133 ± 0.296
2.466TyrLeu: 2.466 ± 0.44
0.267TyrMet: 0.267 ± 0.13
0.8TyrAsn: 0.8 ± 0.232
1.0TyrPro: 1.0 ± 0.221
1.333TyrGln: 1.333 ± 0.34
1.933TyrArg: 1.933 ± 0.313
2.466TyrSer: 2.466 ± 0.347
1.799TyrThr: 1.799 ± 0.318
1.599TyrVal: 1.599 ± 0.335
0.533TyrTrp: 0.533 ± 0.189
0.733TyrTyr: 0.733 ± 0.235
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (15006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski