Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces; Kluyveromyces lactis

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6CVA8|HSE1_KLULA Class E vacuolar protein-sorting machinery protein HSE1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=HSE1 PE=3 SV=1
MM1 pKa = 6.79KK2 pKa = 9.98TSVVNVTILLACGVPLASALRR23 pKa = 11.84TVTSDD28 pKa = 3.22YY29 pKa = 11.59DD30 pKa = 4.15VISDD34 pKa = 3.77TVLDD38 pKa = 3.55VGGAIFNGNVQIEE51 pKa = 4.17EE52 pKa = 4.36DD53 pKa = 3.55KK54 pKa = 11.2FLALVSGTTEE64 pKa = 3.95YY65 pKa = 10.85FNKK68 pKa = 10.55DD69 pKa = 3.97LIVDD73 pKa = 4.17GALYY77 pKa = 10.13IGSKK81 pKa = 9.63IQTLGLDD88 pKa = 3.69VKK90 pKa = 11.28VLGSTQKK97 pKa = 10.02IQNTGTIVLNGHH109 pKa = 5.72NTTLPSTYY117 pKa = 9.46YY118 pKa = 9.79WVGEE122 pKa = 4.1SFKK125 pKa = 11.19NDD127 pKa = 3.02GEE129 pKa = 4.57IYY131 pKa = 10.14WIAQSSLIGSLYY143 pKa = 10.35EE144 pKa = 4.54IIPSSSFEE152 pKa = 4.4NNGLLSFTHH161 pKa = 5.9EE162 pKa = 4.22NGRR165 pKa = 11.84KK166 pKa = 9.93GGTLTLGNLGSAITNSGTICLKK188 pKa = 10.43GVVYY192 pKa = 9.93KK193 pKa = 10.31QSGMVSGEE201 pKa = 4.2SGCISIGDD209 pKa = 3.89EE210 pKa = 4.2GLFWAYY216 pKa = 10.73NGYY219 pKa = 9.42QLTDD223 pKa = 3.1QTIYY227 pKa = 10.83LSSDD231 pKa = 3.0SAALRR236 pKa = 11.84LEE238 pKa = 4.37ALGSPTFVYY247 pKa = 10.38KK248 pKa = 10.81VVGFGKK254 pKa = 10.16QKK256 pKa = 10.65VGLAVAIRR264 pKa = 11.84EE265 pKa = 4.06FQYY268 pKa = 11.38DD269 pKa = 4.07EE270 pKa = 4.07DD271 pKa = 4.19TGILTVSSLLITYY284 pKa = 10.28KK285 pKa = 10.72LDD287 pKa = 3.05IGKK290 pKa = 10.6GYY292 pKa = 10.7DD293 pKa = 3.01RR294 pKa = 11.84TKK296 pKa = 10.8FYY298 pKa = 11.34VDD300 pKa = 5.43DD301 pKa = 3.98IDD303 pKa = 5.37FGAGYY308 pKa = 8.36ITVLNGGIYY317 pKa = 10.44YY318 pKa = 10.64SGDD321 pKa = 3.24VPTFEE326 pKa = 5.26IPEE329 pKa = 4.3ACQPCPEE336 pKa = 4.12TAPWYY341 pKa = 8.92TDD343 pKa = 3.53SNPSDD348 pKa = 3.48VSTISDD354 pKa = 4.12DD355 pKa = 3.72NTSTVIDD362 pKa = 3.76QSSTEE367 pKa = 4.52PIPTTTDD374 pKa = 2.89NGSGSGSGSGSGSASATSTTSNDD397 pKa = 3.07GSGSDD402 pKa = 3.93GSGSGSGSASATSTTSNDD420 pKa = 3.07GSGSDD425 pKa = 3.93GSGSGSGSASATTTASDD442 pKa = 3.48NGSGSGSDD450 pKa = 3.34SGSGSGSDD458 pKa = 4.06SNNGSNSGSNSGSTNGSGSSNDD480 pKa = 3.21SSSNDD485 pKa = 3.03GSGSDD490 pKa = 3.93GSGSGSGSGSASATTTASDD509 pKa = 3.48NGSGSGSGSGSASATSTTSNDD530 pKa = 3.07GSGSDD535 pKa = 3.93GSGSGSGSASATTTASDD552 pKa = 3.48NGSGSGSDD560 pKa = 3.34SGSGSGSGSDD570 pKa = 4.06SNNGSNSGSNSGSTNGSGSSNDD592 pKa = 3.21SSSNDD597 pKa = 3.03GSGSDD602 pKa = 3.93GSGSGSGSGSASATTTASEE621 pKa = 4.23NGSGSGSGSGSASGSASGSASATGTDD647 pKa = 3.73NDD649 pKa = 4.1SSSGSGSDD657 pKa = 3.2SGSGSGSDD665 pKa = 4.06SNNGSNSSSNSGSTNGSGSNNDD687 pKa = 3.28SSSGSSSGSSSNNGSDD703 pKa = 4.25SSNTTYY709 pKa = 8.65PTIKK713 pKa = 7.94TTTKK717 pKa = 10.2AGASSTSATSSDD729 pKa = 3.0GSTDD733 pKa = 3.34AKK735 pKa = 8.35TTATASIYY743 pKa = 10.48TGGAALASAGTGLAAFAIGALLII766 pKa = 4.35

Molecular weight: 73.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6CPF8|Q6CPF8_KLULA Elongator complex protein 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=KLLA0_E05237g PE=3 SV=1
MM1 pKa = 7.83AKK3 pKa = 8.75RR4 pKa = 11.84TKK6 pKa = 10.44KK7 pKa = 10.64VGITGKK13 pKa = 10.38YY14 pKa = 7.24GVRR17 pKa = 11.84YY18 pKa = 9.34GSSLRR23 pKa = 11.84RR24 pKa = 11.84QVKK27 pKa = 9.69KK28 pKa = 11.2LEE30 pKa = 4.24VQQHH34 pKa = 4.32ATYY37 pKa = 10.74NCSFCGKK44 pKa = 7.69TCVKK48 pKa = 10.36RR49 pKa = 11.84GAAGVWSCSSCNRR62 pKa = 11.84TIAGGAYY69 pKa = 8.84TLSTAAAATVRR80 pKa = 11.84STIRR84 pKa = 11.84RR85 pKa = 11.84LRR87 pKa = 11.84DD88 pKa = 3.06MAEE91 pKa = 3.6AA92 pKa = 4.27

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5071

0

5071

2461417

39

4915

485.4

54.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.595 ± 0.03

1.236 ± 0.012

6.057 ± 0.026

6.699 ± 0.038

4.384 ± 0.025

5.097 ± 0.03

2.188 ± 0.014

6.355 ± 0.027

7.079 ± 0.031

9.702 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.013

5.565 ± 0.023

4.303 ± 0.027

4.2 ± 0.03

4.352 ± 0.022

8.83 ± 0.041

5.83 ± 0.031

6.001 ± 0.023

1.089 ± 0.011

3.358 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski