Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; Desulfovibrio desulfuricans

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2345 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8J0F0|B8J0F0_DESDA Uncharacterized protein OS=Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB) OX=525146 GN=Ddes_1325 PE=4 SV=1
MM1 pKa = 7.36TGISFEE7 pKa = 4.32GLSFSPLPTDD17 pKa = 4.62FLSTFNDD24 pKa = 4.15LLWALHH30 pKa = 6.99DD31 pKa = 4.56GGTSIEE37 pKa = 4.15PPEE40 pKa = 5.22LVTQLHH46 pKa = 6.89DD47 pKa = 3.66PAAPFAAPLAATLAEE62 pKa = 4.31TDD64 pKa = 4.41AEE66 pKa = 4.39HH67 pKa = 6.43HH68 pKa = 6.13TQDD71 pKa = 3.96AQHH74 pKa = 6.88AAQDD78 pKa = 4.03SAQDD82 pKa = 4.01TTWTAHH88 pKa = 7.08DD89 pKa = 5.15GPTLLADD96 pKa = 4.57DD97 pKa = 4.79SLNAAFNAQTDD108 pKa = 3.87NAGTQTDD115 pKa = 3.99VFEE118 pKa = 5.51PDD120 pKa = 2.56ATAQTRR126 pKa = 11.84FAMDD130 pKa = 3.57EE131 pKa = 4.12EE132 pKa = 5.05EE133 pKa = 4.29EE134 pKa = 4.66GQVQPLFVFLDD145 pKa = 3.59DD146 pKa = 4.57HH147 pKa = 6.4ATDD150 pKa = 4.15TSASMFLEE158 pKa = 4.42EE159 pKa = 6.06GDD161 pKa = 4.15DD162 pKa = 3.84ALHH165 pKa = 6.34NGYY168 pKa = 10.35LGTEE172 pKa = 4.48GEE174 pKa = 4.25NSGDD178 pKa = 3.29FAGVHH183 pKa = 5.12TSITLTYY190 pKa = 10.23GDD192 pKa = 4.79EE193 pKa = 4.36SLDD196 pKa = 3.81SLFTAAGGQDD206 pKa = 3.56TEE208 pKa = 4.34KK209 pKa = 11.02GGTGLEE215 pKa = 3.84GDD217 pKa = 4.38ALHH220 pKa = 7.11GMGLADD226 pKa = 4.59MNTALDD232 pKa = 3.87QNPLAGSEE240 pKa = 4.17ATGGSAAVAGGVVTGTNEE258 pKa = 3.98TGNVPSVMDD267 pKa = 3.94SCQEE271 pKa = 4.01ATDD274 pKa = 3.58SAARR278 pKa = 11.84EE279 pKa = 4.11MTSCC283 pKa = 4.68

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8J2V5|B8J2V5_DESDA Cobalt-precorrin-5B C(1)-methyltransferase OS=Desulfovibrio desulfuricans (strain ATCC 27774 / DSM 6949 / MB) OX=525146 GN=cbiD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84AVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2345

0

2345

796692

31

3091

339.7

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.296 ± 0.07

1.606 ± 0.029

5.156 ± 0.047

5.86 ± 0.052

3.64 ± 0.039

8.381 ± 0.054

2.231 ± 0.025

4.624 ± 0.049

3.875 ± 0.04

10.797 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.028

3.004 ± 0.029

5.223 ± 0.041

3.568 ± 0.027

6.506 ± 0.058

5.59 ± 0.031

4.891 ± 0.048

7.101 ± 0.038

1.281 ± 0.022

2.461 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski