Amino acid dipepetide frequency for Acidipila sp. EB88

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.12AlaAla: 17.12 ± 0.156	1.051AlaCys: 1.051 ± 0.035	5.62AlaAsp: 5.62 ± 0.075	6.958AlaGlu: 6.958 ± 0.107	3.904AlaPhe: 3.904 ± 0.06	10.409AlaGly: 10.409 ± 0.122	2.807AlaHis: 2.807 ± 0.047	5.267AlaIle: 5.267 ± 0.06	3.6AlaLys: 3.6 ± 0.073	12.667AlaLeu: 12.667 ± 0.122	2.821AlaMet: 2.821 ± 0.048	3.224AlaAsn: 3.224 ± 0.075	6.443AlaPro: 6.443 ± 0.099	5.342AlaGln: 5.342 ± 0.076	7.257AlaArg: 7.257 ± 0.091	7.579AlaSer: 7.579 ± 0.092	7.265AlaThr: 7.265 ± 0.094	8.404AlaVal: 8.404 ± 0.098	1.522AlaTrp: 1.522 ± 0.036	2.589AlaTyr: 2.589 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.065CysAla: 1.065 ± 0.035	0.17CysCys: 0.17 ± 0.014	0.424CysAsp: 0.424 ± 0.021	0.402CysGlu: 0.402 ± 0.017	0.403CysPhe: 0.403 ± 0.019	0.924CysGly: 0.924 ± 0.027	0.212CysHis: 0.212 ± 0.014	0.362CysIle: 0.362 ± 0.016	0.174CysLys: 0.174 ± 0.011	0.843CysLeu: 0.843 ± 0.03	0.212CysMet: 0.212 ± 0.013	0.229CysAsn: 0.229 ± 0.014	0.465CysPro: 0.465 ± 0.022	0.22CysGln: 0.22 ± 0.014	0.528CysArg: 0.528 ± 0.025	0.625CysSer: 0.625 ± 0.025	0.543CysThr: 0.543 ± 0.024	0.668CysVal: 0.668 ± 0.026	0.138CysTrp: 0.138 ± 0.011	0.245CysTyr: 0.245 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.308AspAla: 6.308 ± 0.08	0.378AspCys: 0.378 ± 0.016	2.206AspAsp: 2.206 ± 0.051	2.841AspGlu: 2.841 ± 0.055	1.848AspPhe: 1.848 ± 0.043	4.382AspGly: 4.382 ± 0.077	1.211AspHis: 1.211 ± 0.032	1.956AspIle: 1.956 ± 0.04	1.477AspLys: 1.477 ± 0.042	4.954AspLeu: 4.954 ± 0.072	0.903AspMet: 0.903 ± 0.025	1.247AspAsn: 1.247 ± 0.036	3.251AspPro: 3.251 ± 0.053	1.807AspGln: 1.807 ± 0.044	3.295AspArg: 3.295 ± 0.059	2.495AspSer: 2.495 ± 0.044	2.766AspThr: 2.766 ± 0.045	3.59AspVal: 3.59 ± 0.059	0.801AspTrp: 0.801 ± 0.026	1.486AspTyr: 1.486 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.586GluAla: 6.586 ± 0.092	0.366GluCys: 0.366 ± 0.018	2.445GluAsp: 2.445 ± 0.056	2.874GluGlu: 2.874 ± 0.07	1.697GluPhe: 1.697 ± 0.043	3.645GluGly: 3.645 ± 0.053	1.623GluHis: 1.623 ± 0.043	2.678GluIle: 2.678 ± 0.051	1.882GluLys: 1.882 ± 0.043	5.554GluLeu: 5.554 ± 0.086	1.179GluMet: 1.179 ± 0.032	1.514GluAsn: 1.514 ± 0.032	2.725GluPro: 2.725 ± 0.055	3.091GluGln: 3.091 ± 0.056	4.14GluArg: 4.14 ± 0.072	2.719GluSer: 2.719 ± 0.054	2.94GluThr: 2.94 ± 0.054	3.531GluVal: 3.531 ± 0.054	0.699GluTrp: 0.699 ± 0.021	1.288GluTyr: 1.288 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	4.636PheAla: 4.636 ± 0.077	0.396PheCys: 0.396 ± 0.018	2.21PheAsp: 2.21 ± 0.037	1.875PheGlu: 1.875 ± 0.039	1.568PhePhe: 1.568 ± 0.044	3.407PheGly: 3.407 ± 0.067	1.027PheHis: 1.027 ± 0.028	1.224PheIle: 1.224 ± 0.037	0.862PheLys: 0.862 ± 0.028	3.603PheLeu: 3.603 ± 0.064	0.623PheMet: 0.623 ± 0.022	1.284PheAsn: 1.284 ± 0.044	1.721PhePro: 1.721 ± 0.042	1.284PheGln: 1.284 ± 0.035	2.231PheArg: 2.231 ± 0.042	2.505PheSer: 2.505 ± 0.05	2.363PheThr: 2.363 ± 0.056	2.617PheVal: 2.617 ± 0.044	0.521PheTrp: 0.521 ± 0.02	1.13PheTyr: 1.13 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	8.952GlyAla: 8.952 ± 0.098	0.821GlyCys: 0.821 ± 0.027	3.902GlyAsp: 3.902 ± 0.059	4.012GlyGlu: 4.012 ± 0.063	3.452GlyPhe: 3.452 ± 0.057	6.913GlyGly: 6.913 ± 0.119	1.826GlyHis: 1.826 ± 0.04	4.071GlyIle: 4.071 ± 0.057	3.163GlyLys: 3.163 ± 0.057	7.42GlyLeu: 7.42 ± 0.089	1.959GlyMet: 1.959 ± 0.041	2.656GlyAsn: 2.656 ± 0.068	3.317GlyPro: 3.317 ± 0.056	2.943GlyGln: 2.943 ± 0.053	5.009GlyArg: 5.009 ± 0.073	5.759GlySer: 5.759 ± 0.087	5.78GlyThr: 5.78 ± 0.087	5.955GlyVal: 5.955 ± 0.08	1.375GlyTrp: 1.375 ± 0.039	2.377GlyTyr: 2.377 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.985HisAla: 2.985 ± 0.051	0.261HisCys: 0.261 ± 0.016	1.202HisAsp: 1.202 ± 0.034	1.165HisGlu: 1.165 ± 0.032	1.065HisPhe: 1.065 ± 0.027	2.263HisGly: 2.263 ± 0.043	0.649HisHis: 0.649 ± 0.024	0.977HisIle: 0.977 ± 0.031	0.595HisLys: 0.595 ± 0.025	2.42HisLeu: 2.42 ± 0.043	0.5HisMet: 0.5 ± 0.025	0.663HisAsn: 0.663 ± 0.026	1.639HisPro: 1.639 ± 0.041	0.812HisGln: 0.812 ± 0.022	1.525HisArg: 1.525 ± 0.039	1.435HisSer: 1.435 ± 0.033	1.443HisThr: 1.443 ± 0.04	1.727HisVal: 1.727 ± 0.037	0.465HisTrp: 0.465 ± 0.02	0.738HisTyr: 0.738 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.795IleAla: 5.795 ± 0.066	0.404IleCys: 0.404 ± 0.019	2.684IleAsp: 2.684 ± 0.054	2.694IleGlu: 2.694 ± 0.062	1.531IlePhe: 1.531 ± 0.033	3.688IleGly: 3.688 ± 0.058	1.018IleHis: 1.018 ± 0.031	1.393IleIle: 1.393 ± 0.04	1.158IleLys: 1.158 ± 0.035	4.033IleLeu: 4.033 ± 0.067	0.584IleMet: 0.584 ± 0.022	1.387IleAsn: 1.387 ± 0.038	2.313IlePro: 2.313 ± 0.046	1.388IleGln: 1.388 ± 0.037	2.649IleArg: 2.649 ± 0.051	2.611IleSer: 2.611 ± 0.052	2.811IleThr: 2.811 ± 0.061	3.287IleVal: 3.287 ± 0.055	0.479IleTrp: 0.479 ± 0.023	1.102IleTyr: 1.102 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.379LysAla: 3.379 ± 0.065	0.14LysCys: 0.14 ± 0.012	1.498LysAsp: 1.498 ± 0.042	1.515LysGlu: 1.515 ± 0.045	0.784LysPhe: 0.784 ± 0.028	2.102LysGly: 2.102 ± 0.053	0.789LysHis: 0.789 ± 0.027	1.333LysIle: 1.333 ± 0.034	1.211LysLys: 1.211 ± 0.042	3.322LysLeu: 3.322 ± 0.056	0.715LysMet: 0.715 ± 0.025	0.917LysAsn: 0.917 ± 0.03	2.024LysPro: 2.024 ± 0.043	1.508LysGln: 1.508 ± 0.04	1.967LysArg: 1.967 ± 0.044	1.62LysSer: 1.62 ± 0.044	1.846LysThr: 1.846 ± 0.041	2.108LysVal: 2.108 ± 0.044	0.318LysTrp: 0.318 ± 0.014	0.781LysTyr: 0.781 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	12.99LeuAla: 12.99 ± 0.136	1.112LeuCys: 1.112 ± 0.032	5.188LeuAsp: 5.188 ± 0.072	5.501LeuGlu: 5.501 ± 0.074	3.707LeuPhe: 3.707 ± 0.067	7.713LeuGly: 7.713 ± 0.088	2.797LeuHis: 2.797 ± 0.051	3.874LeuIle: 3.874 ± 0.072	3.073LeuLys: 3.073 ± 0.066	12.105LeuLeu: 12.105 ± 0.183	1.802LeuMet: 1.802 ± 0.041	2.998LeuAsn: 2.998 ± 0.068	6.358LeuPro: 6.358 ± 0.074	4.021LeuGln: 4.021 ± 0.063	7.545LeuArg: 7.545 ± 0.099	6.416LeuSer: 6.416 ± 0.082	5.664LeuThr: 5.664 ± 0.079	7.086LeuVal: 7.086 ± 0.093	1.278LeuTrp: 1.278 ± 0.04	2.456LeuTyr: 2.456 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.403MetAla: 2.403 ± 0.056	0.168MetCys: 0.168 ± 0.013	0.965MetAsp: 0.965 ± 0.032	1.008MetGlu: 1.008 ± 0.031	0.596MetPhe: 0.596 ± 0.023	1.387MetGly: 1.387 ± 0.035	0.629MetHis: 0.629 ± 0.023	0.835MetIle: 0.835 ± 0.026	0.715MetLys: 0.715 ± 0.023	2.5MetLeu: 2.5 ± 0.05	0.46MetMet: 0.46 ± 0.021	0.606MetAsn: 0.606 ± 0.022	1.447MetPro: 1.447 ± 0.032	1.069MetGln: 1.069 ± 0.033	1.671MetArg: 1.671 ± 0.042	1.236MetSer: 1.236 ± 0.03	1.171MetThr: 1.171 ± 0.031	1.434MetVal: 1.434 ± 0.034	0.201MetTrp: 0.201 ± 0.013	0.384MetTyr: 0.384 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.359AsnAla: 3.359 ± 0.06	0.251AsnCys: 0.251 ± 0.015	1.466AsnAsp: 1.466 ± 0.04	1.379AsnGlu: 1.379 ± 0.035	1.285AsnPhe: 1.285 ± 0.041	2.777AsnGly: 2.777 ± 0.068	0.686AsnHis: 0.686 ± 0.024	1.377AsnIle: 1.377 ± 0.032	0.772AsnLys: 0.772 ± 0.025	2.939AsnLeu: 2.939 ± 0.059	0.533AsnMet: 0.533 ± 0.02	1.183AsnAsn: 1.183 ± 0.048	2.085AsnPro: 2.085 ± 0.062	1.255AsnGln: 1.255 ± 0.039	1.668AsnArg: 1.668 ± 0.045	1.751AsnSer: 1.751 ± 0.045	1.889AsnThr: 1.889 ± 0.058	2.128AsnVal: 2.128 ± 0.057	0.409AsnTrp: 0.409 ± 0.019	1.14AsnTyr: 1.14 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	7.625ProAla: 7.625 ± 0.101	0.391ProCys: 0.391 ± 0.018	3.115ProAsp: 3.115 ± 0.056	3.537ProGlu: 3.537 ± 0.061	2.067ProPhe: 2.067 ± 0.042	5.173ProGly: 5.173 ± 0.07	1.24ProHis: 1.24 ± 0.034	2.079ProIle: 2.079 ± 0.039	1.507ProLys: 1.507 ± 0.04	5.259ProLeu: 5.259 ± 0.069	1.194ProMet: 1.194 ± 0.03	1.566ProAsn: 1.566 ± 0.047	2.679ProPro: 2.679 ± 0.068	2.105ProGln: 2.105 ± 0.043	3.091ProArg: 3.091 ± 0.054	3.535ProSer: 3.535 ± 0.058	3.159ProThr: 3.159 ± 0.054	4.477ProVal: 4.477 ± 0.069	0.785ProTrp: 0.785 ± 0.034	1.293ProTyr: 1.293 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	4.834GlnAla: 4.834 ± 0.069	0.287GlnCys: 0.287 ± 0.016	1.702GlnAsp: 1.702 ± 0.039	1.87GlnGlu: 1.87 ± 0.041	1.411GlnPhe: 1.411 ± 0.037	3.083GlnGly: 3.083 ± 0.061	1.067GlnHis: 1.067 ± 0.032	1.825GlnIle: 1.825 ± 0.04	1.199GlnLys: 1.199 ± 0.037	3.935GlnLeu: 3.935 ± 0.06	0.961GlnMet: 0.961 ± 0.028	1.252GlnAsn: 1.252 ± 0.042	2.587GlnPro: 2.587 ± 0.048	2.652GlnGln: 2.652 ± 0.058	2.746GlnArg: 2.746 ± 0.046	2.465GlnSer: 2.465 ± 0.048	2.309GlnThr: 2.309 ± 0.05	2.953GlnVal: 2.953 ± 0.056	0.567GlnTrp: 0.567 ± 0.021	1.169GlnTyr: 1.169 ± 0.036	0.0GlnXaa: 0.0 ± 0.0
Arg	6.996ArgAla: 6.996 ± 0.096	0.562ArgCys: 0.562 ± 0.021	3.135ArgAsp: 3.135 ± 0.057	3.979ArgGlu: 3.979 ± 0.081	2.733ArgPhe: 2.733 ± 0.046	4.205ArgGly: 4.205 ± 0.062	1.535ArgHis: 1.535 ± 0.036	3.295ArgIle: 3.295 ± 0.067	2.194ArgLys: 2.194 ± 0.048	6.533ArgLeu: 6.533 ± 0.1	1.752ArgMet: 1.752 ± 0.041	2.118ArgAsn: 2.118 ± 0.046	3.038ArgPro: 3.038 ± 0.061	2.475ArgGln: 2.475 ± 0.044	4.497ArgArg: 4.497 ± 0.085	4.038ArgSer: 4.038 ± 0.055	3.749ArgThr: 3.749 ± 0.061	4.771ArgVal: 4.771 ± 0.072	1.078ArgTrp: 1.078 ± 0.028	1.899ArgTyr: 1.899 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	7.049SerAla: 7.049 ± 0.079	0.526SerCys: 0.526 ± 0.023	2.825SerAsp: 2.825 ± 0.047	2.932SerGlu: 2.932 ± 0.051	2.594SerPhe: 2.594 ± 0.05	5.676SerGly: 5.676 ± 0.086	1.293SerHis: 1.293 ± 0.031	2.975SerIle: 2.975 ± 0.053	1.713SerLys: 1.713 ± 0.041	6.502SerLeu: 6.502 ± 0.08	1.409SerMet: 1.409 ± 0.036	1.961SerAsn: 1.961 ± 0.045	3.452SerPro: 3.452 ± 0.06	2.043SerGln: 2.043 ± 0.048	3.579SerArg: 3.579 ± 0.06	4.277SerSer: 4.277 ± 0.086	3.988SerThr: 3.988 ± 0.074	4.489SerVal: 4.489 ± 0.061	0.854SerTrp: 0.854 ± 0.028	1.741SerTyr: 1.741 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	7.121ThrAla: 7.121 ± 0.097	0.466ThrCys: 0.466 ± 0.022	2.724ThrAsp: 2.724 ± 0.051	2.734ThrGlu: 2.734 ± 0.047	2.164ThrPhe: 2.164 ± 0.046	5.582ThrGly: 5.582 ± 0.085	1.393ThrHis: 1.393 ± 0.033	2.812ThrIle: 2.812 ± 0.055	1.423ThrLys: 1.423 ± 0.036	6.801ThrLeu: 6.801 ± 0.083	1.123ThrMet: 1.123 ± 0.033	1.845ThrAsn: 1.845 ± 0.056	4.15ThrPro: 4.15 ± 0.069	2.156ThrGln: 2.156 ± 0.051	3.448ThrArg: 3.448 ± 0.057	3.607ThrSer: 3.607 ± 0.068	3.849ThrThr: 3.849 ± 0.07	4.671ThrVal: 4.671 ± 0.077	0.786ThrTrp: 0.786 ± 0.026	1.623ThrTyr: 1.623 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	8.516ValAla: 8.516 ± 0.115	0.732ValCys: 0.732 ± 0.027	3.82ValAsp: 3.82 ± 0.07	4.026ValGlu: 4.026 ± 0.068	2.647ValPhe: 2.647 ± 0.052	5.009ValGly: 5.009 ± 0.083	1.759ValHis: 1.759 ± 0.037	3.053ValIle: 3.053 ± 0.055	1.955ValLys: 1.955 ± 0.041	8.06ValLeu: 8.06 ± 0.101	1.333ValMet: 1.333 ± 0.032	2.136ValAsn: 2.136 ± 0.05	4.133ValPro: 4.133 ± 0.056	2.878ValGln: 2.878 ± 0.052	4.895ValArg: 4.895 ± 0.08	4.497ValSer: 4.497 ± 0.059	4.391ValThr: 4.391 ± 0.075	5.534ValVal: 5.534 ± 0.079	0.884ValTrp: 0.884 ± 0.029	1.781ValTyr: 1.781 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.181TrpAla: 1.181 ± 0.034	0.166TrpCys: 0.166 ± 0.012	0.67TrpAsp: 0.67 ± 0.025	0.594TrpGlu: 0.594 ± 0.025	0.537TrpPhe: 0.537 ± 0.023	0.919TrpGly: 0.919 ± 0.038	0.38TrpHis: 0.38 ± 0.019	0.632TrpIle: 0.632 ± 0.025	0.513TrpLys: 0.513 ± 0.022	1.559TrpLeu: 1.559 ± 0.044	0.397TrpMet: 0.397 ± 0.02	0.575TrpAsn: 0.575 ± 0.024	0.625TrpPro: 0.625 ± 0.025	0.752TrpGln: 0.752 ± 0.026	0.945TrpArg: 0.945 ± 0.029	0.996TrpSer: 0.996 ± 0.033	0.841TrpThr: 0.841 ± 0.029	0.875TrpVal: 0.875 ± 0.029	0.248TrpTrp: 0.248 ± 0.015	0.367TrpTyr: 0.367 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.951TyrAla: 2.951 ± 0.048	0.204TyrCys: 0.204 ± 0.013	1.399TyrAsp: 1.399 ± 0.038	1.282TyrGlu: 1.282 ± 0.036	1.097TyrPhe: 1.097 ± 0.034	2.407TyrGly: 2.407 ± 0.052	0.586TyrHis: 0.586 ± 0.02	0.989TyrIle: 0.989 ± 0.03	0.694TyrLys: 0.694 ± 0.029	2.678TyrLeu: 2.678 ± 0.049	0.425TyrMet: 0.425 ± 0.017	0.971TyrAsn: 0.971 ± 0.037	1.426TyrPro: 1.426 ± 0.038	1.075TyrGln: 1.075 ± 0.034	1.897TyrArg: 1.897 ± 0.041	1.707TyrSer: 1.707 ± 0.042	1.71TyrThr: 1.71 ± 0.05	1.731TyrVal: 1.731 ± 0.041	0.362TyrTrp: 0.362 ± 0.017	0.758TyrTyr: 0.758 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3402 proteins (1244068 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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