Erwinia phage phiEa21-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Kolesnikvirus; Erwinia virus Ea214

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8QTS3|B8QTS3_9CAUD Hypothetical phage protein OS=Erwinia phage phiEa21-4 OX=557393 GN=25 PE=4 SV=1
MM1 pKa = 7.37LCVHH5 pKa = 6.44GTTLEE10 pKa = 4.03NFKK13 pKa = 10.87MMIANEE19 pKa = 4.32GKK21 pKa = 9.68PSGAWNVSEE30 pKa = 5.22CDD32 pKa = 3.09AATYY36 pKa = 8.16FYY38 pKa = 10.42PLDD41 pKa = 4.59KK42 pKa = 10.27IAKK45 pKa = 8.9AEE47 pKa = 4.08CLEE50 pKa = 4.53ADD52 pKa = 4.22DD53 pKa = 5.54FDD55 pKa = 5.92SEE57 pKa = 5.53SEE59 pKa = 4.19YY60 pKa = 11.19QDD62 pKa = 5.8AILQAAKK69 pKa = 10.12RR70 pKa = 11.84HH71 pKa = 5.09ARR73 pKa = 11.84GNAMTTAAIAEE84 pKa = 4.47RR85 pKa = 11.84DD86 pKa = 3.38TKK88 pKa = 11.13LIVFLADD95 pKa = 3.44VPEE98 pKa = 4.69HH99 pKa = 6.58LLEE102 pKa = 6.12DD103 pKa = 5.39DD104 pKa = 4.21YY105 pKa = 12.15SCDD108 pKa = 3.28NMADD112 pKa = 3.01IASFIHH118 pKa = 6.78EE119 pKa = 4.9DD120 pKa = 3.55DD121 pKa = 4.92FKK123 pKa = 11.77VEE125 pKa = 4.21MIQRR129 pKa = 11.84IWQYY133 pKa = 10.4SFFGIFSPYY142 pKa = 9.48CVQPDD147 pKa = 4.02NPYY150 pKa = 8.74MMSMSYY156 pKa = 10.36HH157 pKa = 6.65LEE159 pKa = 3.66QMARR163 pKa = 11.84AIQNSNAYY171 pKa = 10.37LYY173 pKa = 9.15DD174 pKa = 4.02TIEE177 pKa = 4.12NSLDD181 pKa = 3.28EE182 pKa = 4.78SEE184 pKa = 4.25EE185 pKa = 4.04QKK187 pKa = 11.24GLEE190 pKa = 3.86FLINII195 pKa = 4.85

Molecular weight: 22.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8QTV5|B8QTV5_9CAUD Conserved structural protein OS=Erwinia phage phiEa21-4 OX=557393 GN=57 PE=4 SV=1
MM1 pKa = 6.97VKK3 pKa = 10.15RR4 pKa = 11.84VIGEE8 pKa = 3.88GRR10 pKa = 11.84NILMKK15 pKa = 10.38AVKK18 pKa = 10.11NIAKK22 pKa = 8.66STVKK26 pKa = 10.58VGFFAEE32 pKa = 4.2QGKK35 pKa = 9.81HH36 pKa = 4.7YY37 pKa = 11.04SGFSYY42 pKa = 9.78PALMYY47 pKa = 10.18LHH49 pKa = 6.91EE50 pKa = 4.36VHH52 pKa = 7.42GIPASNGKK60 pKa = 8.93VYY62 pKa = 10.59RR63 pKa = 11.84RR64 pKa = 11.84AFEE67 pKa = 3.69TAMQIDD73 pKa = 4.46RR74 pKa = 11.84KK75 pKa = 10.57LLIAQAQKK83 pKa = 10.66NLKK86 pKa = 10.12RR87 pKa = 11.84MLSTGQTNPDD97 pKa = 3.19LVLSQFGKK105 pKa = 10.66DD106 pKa = 3.44SIKK109 pKa = 10.66SLKK112 pKa = 10.17KK113 pKa = 10.29VFGNPSLLPPNTPSTIKK130 pKa = 10.81SKK132 pKa = 10.83GGRR135 pKa = 11.84NTPLVDD141 pKa = 3.7TSDD144 pKa = 4.05LVDD147 pKa = 3.34NLAYY151 pKa = 8.49KK152 pKa = 9.7TSNRR156 pKa = 11.84GGLRR160 pKa = 11.84KK161 pKa = 9.68

Molecular weight: 17.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

25224

44

1157

213.8

23.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.862 ± 0.316

1.142 ± 0.12

6.343 ± 0.169

6.288 ± 0.223

4.135 ± 0.142

6.803 ± 0.262

2.026 ± 0.158

5.943 ± 0.139

7.021 ± 0.242

8.012 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.149

5.209 ± 0.174

3.172 ± 0.162

3.631 ± 0.153

4.444 ± 0.187

6.72 ± 0.234

6.252 ± 0.316

6.898 ± 0.181

1.265 ± 0.145

4.016 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski