Simplicispira sp. 125

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Simplicispira; unclassified Simplicispira

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3567 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1C253|A0A2U1C253_9BURK 50S ribosomal protein L28 OS=Simplicispira sp. 125 OX=2135643 GN=rpmB PE=3 SV=1
MM1 pKa = 7.63TDD3 pKa = 3.07SLTWMCLLCGWIYY16 pKa = 11.25DD17 pKa = 4.02EE18 pKa = 5.52ARR20 pKa = 11.84GSPEE24 pKa = 3.58YY25 pKa = 10.31GIPPGTPWDD34 pKa = 3.79QVPADD39 pKa = 4.0WTCPEE44 pKa = 4.28CGAGKK49 pKa = 10.12SVFEE53 pKa = 4.25MVQII57 pKa = 4.41

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1C9T2|A0A2U1C9T2_9BURK Formate dehydrogenase gamma subunit OS=Simplicispira sp. 125 OX=2135643 GN=C8D04_2935 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3567

0

3567

1182683

30

1990

331.6

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.825 ± 0.053

0.958 ± 0.012

5.045 ± 0.028

5.147 ± 0.035

3.432 ± 0.025

8.226 ± 0.036

2.403 ± 0.021

4.243 ± 0.036

3.207 ± 0.04

10.912 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.019

2.569 ± 0.022

5.241 ± 0.033

4.569 ± 0.031

6.655 ± 0.037

5.289 ± 0.029

5.308 ± 0.031

7.682 ± 0.039

1.493 ± 0.017

2.183 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski