Nonlabens ponticola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Nonlabens

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2545 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9N180|A0A3S9N180_9FLAO Uncharacterized protein OS=Nonlabens ponticola OX=2496866 GN=EJ995_01345 PE=4 SV=1
MM1 pKa = 7.32FNKK4 pKa = 10.09IGWFLVIAVTILGCQDD20 pKa = 4.22DD21 pKa = 4.23DD22 pKa = 4.39TEE24 pKa = 4.65FGPVVAPSDD33 pKa = 3.52LSVSFEE39 pKa = 4.14VQGEE43 pKa = 3.99DD44 pKa = 4.8DD45 pKa = 4.48EE46 pKa = 5.24NPNGDD51 pKa = 3.23GTGVVTFSANASNALNYY68 pKa = 8.92TYY70 pKa = 11.18SFGDD74 pKa = 3.42SRR76 pKa = 11.84SSFSSDD82 pKa = 2.3GDD84 pKa = 3.68LEE86 pKa = 4.17HH87 pKa = 7.56RR88 pKa = 11.84YY89 pKa = 8.85VQQGVNTYY97 pKa = 8.15TVTVTATGTGGAATSQTTQVTVFSAFEE124 pKa = 4.69DD125 pKa = 3.6NDD127 pKa = 3.63AKK129 pKa = 10.96QLLTGGSSKK138 pKa = 9.64IWYY141 pKa = 6.48WAAAEE146 pKa = 4.35PGHH149 pKa = 7.39LGVGPNNGDD158 pKa = 3.39PTDD161 pKa = 3.93GNNNFPQFYY170 pKa = 9.58QAAPFEE176 pKa = 4.75KK177 pKa = 10.62NGADD181 pKa = 3.46EE182 pKa = 4.55SLCLYY187 pKa = 10.18EE188 pKa = 4.58DD189 pKa = 3.42QLVFSLNDD197 pKa = 3.64DD198 pKa = 3.88EE199 pKa = 4.94EE200 pKa = 5.0LVYY203 pKa = 10.71QLNNFGQTYY212 pKa = 10.01FNGAFEE218 pKa = 4.56SVVGGSNGFDD228 pKa = 3.1FCYY231 pKa = 10.81DD232 pKa = 3.58FDD234 pKa = 4.37TDD236 pKa = 3.65GVKK239 pKa = 10.44NVTLSPSEE247 pKa = 4.5SIVPDD252 pKa = 3.38SEE254 pKa = 4.57KK255 pKa = 10.77RR256 pKa = 11.84GTTMTFSDD264 pKa = 4.83DD265 pKa = 3.73GFMSYY270 pKa = 10.84YY271 pKa = 10.13INTSVYY277 pKa = 10.49DD278 pKa = 3.62ILEE281 pKa = 4.18LTEE284 pKa = 3.53NRR286 pKa = 11.84MVVRR290 pKa = 11.84GIMNGDD296 pKa = 3.81LAWYY300 pKa = 7.72HH301 pKa = 5.76TFSTDD306 pKa = 4.79DD307 pKa = 3.73PNAEE311 pKa = 4.14QGGDD315 pKa = 3.81DD316 pKa = 3.9NSDD319 pKa = 3.12GSGEE323 pKa = 4.1PEE325 pKa = 4.11GTLVWSDD332 pKa = 3.49EE333 pKa = 4.06FDD335 pKa = 3.45VDD337 pKa = 4.96GAPNPANWTFDD348 pKa = 3.14TGTGSDD354 pKa = 2.8GWGNNEE360 pKa = 3.67QQFYY364 pKa = 10.13TDD366 pKa = 2.96RR367 pKa = 11.84TEE369 pKa = 4.39NIEE372 pKa = 4.08VSNGTLKK379 pKa = 9.02ITARR383 pKa = 11.84DD384 pKa = 3.27EE385 pKa = 4.66DD386 pKa = 3.99FGGRR390 pKa = 11.84SYY392 pKa = 11.03TSSRR396 pKa = 11.84IKK398 pKa = 10.19TEE400 pKa = 3.88GLFEE404 pKa = 4.04FQYY407 pKa = 10.23GTIEE411 pKa = 4.31FSAKK415 pKa = 10.1LPEE418 pKa = 5.59GGGTWPAIWSLGADD432 pKa = 3.74YY433 pKa = 8.5QTNPWPAAGEE443 pKa = 3.85IDD445 pKa = 4.04YY446 pKa = 10.21MEE448 pKa = 6.07HH449 pKa = 6.99IGNTQNTIYY458 pKa = 10.05ATVHH462 pKa = 6.01RR463 pKa = 11.84PGASGGNADD472 pKa = 3.79GGEE475 pKa = 4.04IEE477 pKa = 4.78IEE479 pKa = 4.01NASSEE484 pKa = 4.01FHH486 pKa = 6.53TYY488 pKa = 7.95RR489 pKa = 11.84TIWNEE494 pKa = 3.27DD495 pKa = 3.71TIRR498 pKa = 11.84FFVDD502 pKa = 3.74GEE504 pKa = 4.71LIHH507 pKa = 6.68QVANSDD513 pKa = 3.35AVPFNKK519 pKa = 10.18DD520 pKa = 2.7FFIIMNIAMGGNFGGDD536 pKa = 2.91IDD538 pKa = 4.13PAFTEE543 pKa = 4.22STMEE547 pKa = 3.25VDD549 pKa = 4.35YY550 pKa = 11.35IRR552 pKa = 11.84VYY554 pKa = 10.14QQ555 pKa = 3.67

Molecular weight: 60.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9MZT2|A0A3S9MZT2_9FLAO NAD-dependent epimerase/dehydratase family protein OS=Nonlabens ponticola OX=2496866 GN=EJ995_10485 PE=4 SV=1
MM1 pKa = 6.94AQKK4 pKa = 9.31RR5 pKa = 11.84TYY7 pKa = 10.22QPSKK11 pKa = 9.01RR12 pKa = 11.84KK13 pKa = 9.47RR14 pKa = 11.84RR15 pKa = 11.84NKK17 pKa = 9.49HH18 pKa = 3.94GFRR21 pKa = 11.84EE22 pKa = 4.27RR23 pKa = 11.84MASANGRR30 pKa = 11.84KK31 pKa = 9.04VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 10.09GRR41 pKa = 11.84KK42 pKa = 8.0KK43 pKa = 10.66LSVSTEE49 pKa = 3.69RR50 pKa = 11.84RR51 pKa = 11.84HH52 pKa = 6.27KK53 pKa = 10.62RR54 pKa = 3.14

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2545

0

2545

867875

29

9254

341.0

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.08 ± 0.054

0.658 ± 0.015

6.929 ± 0.206

6.393 ± 0.051

4.703 ± 0.039

6.557 ± 0.071

1.838 ± 0.034

7.337 ± 0.045

6.302 ± 0.088

9.164 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.039

5.507 ± 0.063

3.437 ± 0.032

3.972 ± 0.035

4.145 ± 0.05

6.372 ± 0.043

5.807 ± 0.062

6.469 ± 0.033

0.996 ± 0.02

3.989 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski