Streptococcus satellite phage Javan415

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZPH6|A0A4D5ZPH6_9VIRU HTH cro/C1-type domain-containing protein OS=Streptococcus satellite phage Javan415 OX=2558690 GN=JavanS415_0012 PE=4 SV=1
MM1 pKa = 7.88VNITDD6 pKa = 3.82VKK8 pKa = 10.81QILQFAIDD16 pKa = 3.74AEE18 pKa = 4.38IKK20 pKa = 10.71VFLDD24 pKa = 3.67GGWGVDD30 pKa = 3.37ALLGYY35 pKa = 8.24QSRR38 pKa = 11.84AHH40 pKa = 6.36NDD42 pKa = 2.16IDD44 pKa = 4.33IFVEE48 pKa = 4.03KK49 pKa = 10.57NDD51 pKa = 3.52YY52 pKa = 11.06QNFIEE57 pKa = 4.35IMKK60 pKa = 10.8ANGFYY65 pKa = 9.81EE66 pKa = 3.97IKK68 pKa = 9.96MEE70 pKa = 4.09YY71 pKa = 7.09TTLNHH76 pKa = 5.95TVWEE80 pKa = 4.16DD81 pKa = 3.21LKK83 pKa = 11.51NRR85 pKa = 11.84IIDD88 pKa = 3.56LHH90 pKa = 7.38CFEE93 pKa = 4.89YY94 pKa = 10.57TDD96 pKa = 3.95EE97 pKa = 5.66GEE99 pKa = 3.93ILYY102 pKa = 10.75DD103 pKa = 3.82GDD105 pKa = 4.27CFPVEE110 pKa = 4.2TFSGKK115 pKa = 10.15GRR117 pKa = 11.84IEE119 pKa = 4.42EE120 pKa = 4.37IEE122 pKa = 4.28VSCIEE127 pKa = 4.83PYY129 pKa = 9.55SQVMFHH135 pKa = 6.87LGYY138 pKa = 10.91EE139 pKa = 4.04FDD141 pKa = 4.65EE142 pKa = 4.84NDD144 pKa = 3.22AHH146 pKa = 7.24DD147 pKa = 4.19VKK149 pKa = 11.0LLCEE153 pKa = 4.15TFHH156 pKa = 8.87IEE158 pKa = 4.46IPNEE162 pKa = 3.74YY163 pKa = 10.09RR164 pKa = 3.46

Molecular weight:
19.24 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZSM1|A0A4D5ZSM1_9VIRU Integrase OS=Streptococcus satellite phage Javan415 OX=2558690 GN=JavanS415_0013 PE=3 SV=1
MM1 pKa = 7.39FLHH4 pKa = 6.8FPIILDD10 pKa = 4.48GIIEE14 pKa = 4.21ADD16 pKa = 3.38EE17 pKa = 4.25TFFAISYY24 pKa = 9.35KK25 pKa = 10.68GNHH28 pKa = 6.13SKK30 pKa = 10.92SKK32 pKa = 8.95TFAMPRR38 pKa = 11.84KK39 pKa = 7.79AHH41 pKa = 6.1KK42 pKa = 9.66RR43 pKa = 11.84GHH45 pKa = 4.94STHH48 pKa = 6.35IRR50 pKa = 11.84GLSQEE55 pKa = 4.4KK56 pKa = 10.23VCVPCAVNRR65 pKa = 11.84NGLSISKK72 pKa = 8.26ITNTGRR78 pKa = 11.84VSTRR82 pKa = 11.84DD83 pKa = 3.09LHH85 pKa = 7.18HH86 pKa = 7.07IYY88 pKa = 10.41DD89 pKa = 3.91GRR91 pKa = 11.84IMTNSTLVTDD101 pKa = 4.3KK102 pKa = 10.3MNSYY106 pKa = 10.45VRR108 pKa = 11.84FTNANGIDD116 pKa = 4.04LVQLKK121 pKa = 8.99TGKK124 pKa = 9.91AKK126 pKa = 10.54KK127 pKa = 10.1GIYY130 pKa = 9.6NIQHH134 pKa = 5.91INSYY138 pKa = 10.3HH139 pKa = 5.54SQLKK143 pKa = 9.97RR144 pKa = 11.84FMRR147 pKa = 11.84GFNGVSTKK155 pKa = 10.69YY156 pKa = 10.69LNNYY160 pKa = 7.83LVWNNLVNYY169 pKa = 9.92AKK171 pKa = 10.47EE172 pKa = 3.76SDD174 pKa = 3.53MEE176 pKa = 4.07KK177 pKa = 10.79RR178 pKa = 11.84NIFLTFVLATLKK190 pKa = 9.45TAKK193 pKa = 10.3CRR195 pKa = 11.84DD196 pKa = 3.51LSNRR200 pKa = 11.84PAVPLVAA207 pKa = 5.64

Molecular weight:
23.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2485

61

477

191.2

22.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.473 ± 0.506

0.644 ± 0.191

5.755 ± 0.785

8.571 ± 0.85

4.185 ± 0.33

4.99 ± 0.356

1.73 ± 0.423

7.243 ± 0.596

9.658 ± 0.676

8.813 ± 0.608

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.321

6.278 ± 0.453

3.018 ± 0.407

2.897 ± 0.414

4.99 ± 0.419

4.829 ± 0.566

6.318 ± 0.468

5.835 ± 0.348

0.926 ± 0.143

5.352 ± 0.296

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski