Verticillium longisporum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordar

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20580 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G4L7N3|A0A0G4L7N3_9PEZI Homocitrate synthase OS=Verticillium longisporum OX=100787 GN=BN1708_012205 PE=3 SV=1
MM1 pKa = 6.93VASHH5 pKa = 6.91LKK7 pKa = 10.36LAIGLTAASNVVAFSSIRR25 pKa = 11.84IAPTVEE31 pKa = 3.49AGKK34 pKa = 9.74EE35 pKa = 4.07VEE37 pKa = 4.1VSIVNDD43 pKa = 4.18LEE45 pKa = 5.96DD46 pKa = 4.38SDD48 pKa = 5.6SFDD51 pKa = 3.82AGFEE55 pKa = 3.98KK56 pKa = 10.6FRR58 pKa = 11.84VYY60 pKa = 10.82LATTPPGWGTGPACYY75 pKa = 9.55LVSATVIDD83 pKa = 3.92EE84 pKa = 4.36TSVKK88 pKa = 8.81VTIPPSVVPHH98 pKa = 6.71GSDD101 pKa = 3.11VMISVMEE108 pKa = 4.24FNEE111 pKa = 4.5DD112 pKa = 3.39PSLDD116 pKa = 3.86GPSGFQYY123 pKa = 11.17SNEE126 pKa = 3.78FTLNGGKK133 pKa = 10.31GEE135 pKa = 4.0WSKK138 pKa = 11.67PEE140 pKa = 4.07LEE142 pKa = 4.61GFNIGDD148 pKa = 4.02SDD150 pKa = 4.65TIPCEE155 pKa = 3.98AYY157 pKa = 10.23ACARR161 pKa = 11.84DD162 pKa = 4.14CMVQFYY168 pKa = 10.16PDD170 pKa = 3.44NKK172 pKa = 9.83EE173 pKa = 4.26DD174 pKa = 3.27EE175 pKa = 4.43SAYY178 pKa = 10.11KK179 pKa = 8.78KK180 pKa = 9.34TYY182 pKa = 9.97EE183 pKa = 4.45CTAEE187 pKa = 4.34CPGVDD192 pKa = 3.71YY193 pKa = 9.49PAWDD197 pKa = 3.68SLPGVDD203 pKa = 5.49EE204 pKa = 4.53VPSDD208 pKa = 4.11NDD210 pKa = 3.7GDD212 pKa = 5.14DD213 pKa = 4.3EE214 pKa = 7.21GDD216 pKa = 4.63DD217 pKa = 4.19DD218 pKa = 6.67DD219 pKa = 4.43EE220 pKa = 4.44TASISGATSGTATPSSGAATTILTTTGADD249 pKa = 3.21EE250 pKa = 4.75ASAPTEE256 pKa = 4.35TPSSAGRR263 pKa = 11.84HH264 pKa = 4.53TASLMLLAGIIGMCSIFF281 pKa = 3.82

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G4MVS4|A0A0G4MVS4_9PEZI Uncharacterized protein (Fragment) OS=Verticillium longisporum OX=100787 GN=BN1708_020467 PE=4 SV=1
VV1 pKa = 7.48PLPLSPWAARR11 pKa = 11.84ATLSWRR17 pKa = 11.84SGTTPGPRR25 pKa = 11.84SRR27 pKa = 11.84PSRR30 pKa = 11.84TSPTGVRR37 pKa = 11.84GTTTASGSRR46 pKa = 11.84TSMAVSRR53 pKa = 11.84VTAASRR59 pKa = 11.84AGPSTGRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 3.41

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20580

0

20580

9306748

8

8488

452.2

49.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.711 ± 0.018

1.207 ± 0.01

5.893 ± 0.014

5.941 ± 0.016

3.567 ± 0.012

7.175 ± 0.019

2.462 ± 0.008

4.42 ± 0.012

4.514 ± 0.017

8.739 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.007

3.41 ± 0.009

6.293 ± 0.021

3.973 ± 0.014

6.412 ± 0.018

7.82 ± 0.02

5.967 ± 0.013

6.284 ± 0.013

1.436 ± 0.008

2.534 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski