Lake Sarah-associated circular virus-1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQJ3|A0A140AQJ3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-1 OX=1685735 PE=4 SV=1
MM1 pKa = 7.16SQGIYY6 pKa = 9.63WLLTINQHH14 pKa = 5.28EE15 pKa = 4.51FVPYY19 pKa = 10.13LPAGVSYY26 pKa = 10.82IRR28 pKa = 11.84GQLEE32 pKa = 3.78MANNQGADD40 pKa = 2.8TGYY43 pKa = 10.91LHH45 pKa = 6.57WQLLVVFSRR54 pKa = 11.84KK55 pKa = 9.08CRR57 pKa = 11.84LAAVKK62 pKa = 10.15KK63 pKa = 10.15LLGNTVHH70 pKa = 7.26AEE72 pKa = 4.33LSRR75 pKa = 11.84SAAATDD81 pKa = 4.06YY82 pKa = 10.8VWKK85 pKa = 10.25EE86 pKa = 3.95DD87 pKa = 3.4TRR89 pKa = 11.84VAGTQFEE96 pKa = 4.68LGAMPMQRR104 pKa = 11.84NNPTDD109 pKa = 3.26WDD111 pKa = 4.05TVRR114 pKa = 11.84NNARR118 pKa = 11.84AGRR121 pKa = 11.84LGDD124 pKa = 3.39ISGDD128 pKa = 3.04IYY130 pKa = 10.9VRR132 pKa = 11.84CYY134 pKa = 10.79NQLKK138 pKa = 10.45RR139 pKa = 11.84IACDD143 pKa = 3.12HH144 pKa = 5.78MQADD148 pKa = 3.85PVEE151 pKa = 4.98RR152 pKa = 11.84EE153 pKa = 4.32VVCYY157 pKa = 9.39WGRR160 pKa = 11.84TGTGKK165 pKa = 9.99SRR167 pKa = 11.84RR168 pKa = 11.84AWDD171 pKa = 3.64EE172 pKa = 3.86ASLDD176 pKa = 4.31AYY178 pKa = 10.14PKK180 pKa = 10.45DD181 pKa = 3.65PRR183 pKa = 11.84SKK185 pKa = 10.25FWDD188 pKa = 4.41GYY190 pKa = 9.92RR191 pKa = 11.84GQEE194 pKa = 3.8HH195 pKa = 5.89VVLDD199 pKa = 4.06EE200 pKa = 4.0FRR202 pKa = 11.84GGIDD206 pKa = 2.92IAHH209 pKa = 6.28VLRR212 pKa = 11.84WFDD215 pKa = 3.75RR216 pKa = 11.84YY217 pKa = 10.29PVMVEE222 pKa = 3.84VKK224 pKa = 10.41GSSVPLKK231 pKa = 9.61ATKK234 pKa = 9.7IWITSNLPPEE244 pKa = 4.05SWYY247 pKa = 10.32PEE249 pKa = 4.13LDD251 pKa = 4.1DD252 pKa = 3.99EE253 pKa = 4.86TKK255 pKa = 9.75QALRR259 pKa = 11.84RR260 pKa = 11.84RR261 pKa = 11.84MNITHH266 pKa = 7.51FII268 pKa = 3.93

Molecular weight: 30.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQJ3|A0A140AQJ3_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-1 OX=1685735 PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 10.33SKK4 pKa = 10.45YY5 pKa = 9.59VKK7 pKa = 10.11LAARR11 pKa = 11.84VAPYY15 pKa = 10.36AAIAGRR21 pKa = 11.84QLVRR25 pKa = 11.84YY26 pKa = 8.89AGNRR30 pKa = 11.84AKK32 pKa = 10.69DD33 pKa = 3.63YY34 pKa = 11.2VYY36 pKa = 10.74GKK38 pKa = 10.28SSSKK42 pKa = 9.5PKK44 pKa = 10.64SRR46 pKa = 11.84ISPPKK51 pKa = 9.58AKK53 pKa = 10.71ANFGFRR59 pKa = 11.84GTGKK63 pKa = 10.52LGGFVGRR70 pKa = 11.84GKK72 pKa = 10.34RR73 pKa = 11.84VRR75 pKa = 11.84KK76 pKa = 8.06TKK78 pKa = 10.42FSQAQRR84 pKa = 11.84YY85 pKa = 8.07GVYY88 pKa = 9.71QQVEE92 pKa = 4.41IYY94 pKa = 10.56KK95 pKa = 10.31SVTGSQTLAVGHH107 pKa = 6.21ATNPVKK113 pKa = 10.9VVFGSVAHH121 pKa = 6.68ALLKK125 pKa = 10.89KK126 pKa = 10.27FVEE129 pKa = 4.19RR130 pKa = 11.84DD131 pKa = 3.79AICFSDD137 pKa = 3.31WNTVVVGNTKK147 pKa = 10.37YY148 pKa = 8.32PTGAKK153 pKa = 10.08FYY155 pKa = 10.48ISYY158 pKa = 10.43RR159 pKa = 11.84DD160 pKa = 3.31TDD162 pKa = 3.91AGTMADD168 pKa = 3.97SLVITIGGATTFEE181 pKa = 5.28DD182 pKa = 3.21IALAIYY188 pKa = 7.41TTISTLSSKK197 pKa = 11.16YY198 pKa = 11.03SMQLMYY204 pKa = 10.89AVTGAGLLLTTLYY217 pKa = 11.09LNTAFVKK224 pKa = 10.56LHH226 pKa = 6.1TKK228 pKa = 10.23SALKK232 pKa = 9.76FQNITIDD239 pKa = 3.39TGASTEE245 pKa = 3.92QDD247 pKa = 3.12DD248 pKa = 5.57VRR250 pKa = 11.84NQPLNGKK257 pKa = 9.46AYY259 pKa = 9.1EE260 pKa = 4.44GKK262 pKa = 8.75GTGADD267 pKa = 3.95VYY269 pKa = 10.6WIGAGAASNLVCDD282 pKa = 3.58TAYY285 pKa = 10.67GVLGVSDD292 pKa = 4.33PVEE295 pKa = 5.68DD296 pKa = 3.54IPQPKK301 pKa = 9.16VVQRR305 pKa = 11.84ASKK308 pKa = 9.69FSKK311 pKa = 10.32VRR313 pKa = 11.84IDD315 pKa = 3.16AGAIKK320 pKa = 9.9TSVLTTNHH328 pKa = 7.66KK329 pKa = 10.27IRR331 pKa = 11.84MSDD334 pKa = 3.49LVSMLNSGVEE344 pKa = 4.4GYY346 pKa = 11.16NRR348 pKa = 11.84MGKK351 pKa = 9.52FRR353 pKa = 11.84IFILEE358 pKa = 3.88RR359 pKa = 11.84LIGGNFGSATMQVRR373 pKa = 11.84IEE375 pKa = 4.17HH376 pKa = 6.18QLDD379 pKa = 3.28QSATVIGGNPPYY391 pKa = 10.2MKK393 pKa = 9.68TKK395 pKa = 10.4FVAVQLL401 pKa = 4.08

Molecular weight: 43.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

669

268

401

334.5

37.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.969 ± 0.699

0.897 ± 0.367

5.232 ± 0.916

3.438 ± 1.101

3.438 ± 0.509

8.67 ± 0.975

1.794 ± 0.505

5.082 ± 0.373

6.726 ± 1.387

7.175 ± 0.408

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.136

3.886 ± 0.135

3.587 ± 0.549

4.036 ± 0.042

6.428 ± 1.329

6.278 ± 0.88

6.876 ± 1.019

8.52 ± 0.652

1.794 ± 1.195

4.783 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski