Enterobacter phage ATCEA85

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V4T4|A0A649V4T4_9CAUD Bifunctional DNA primase/polymerase OS=Enterobacter phage ATCEA85 OX=2666249 PE=4 SV=1
MM1 pKa = 7.57IGNIEE6 pKa = 4.25DD7 pKa = 3.74LKK9 pKa = 11.56AFATANGYY17 pKa = 6.78TLEE20 pKa = 4.47DD21 pKa = 3.87EE22 pKa = 4.65EE23 pKa = 4.91LALALRR29 pKa = 11.84QINLWLWSLPWCGEE43 pKa = 4.05KK44 pKa = 10.24VDD46 pKa = 4.57PAQADD51 pKa = 2.94IWPRR55 pKa = 11.84VICDD59 pKa = 4.15DD60 pKa = 4.2YY61 pKa = 11.9NCLEE65 pKa = 4.37YY66 pKa = 10.4EE67 pKa = 4.52VPQQVITFVYY77 pKa = 9.45TAACDD82 pKa = 3.39AAEE85 pKa = 4.35NGVDD89 pKa = 3.54VTTVSSGPKK98 pKa = 9.31KK99 pKa = 10.19KK100 pKa = 10.68AFAITGAISVEE111 pKa = 3.76YY112 pKa = 9.84DD113 pKa = 3.18QNSLYY118 pKa = 11.05SADD121 pKa = 3.92FSPAYY126 pKa = 7.57MQGMIGGWLCGSTNGAMMKK145 pKa = 10.15LQRR148 pKa = 11.84SS149 pKa = 3.72

Molecular weight: 16.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V4C4|A0A649V4C4_9CAUD C-5 cytosine specific DNA methylase OS=Enterobacter phage ATCEA85 OX=2666249 PE=4 SV=1
MM1 pKa = 7.26NMYY4 pKa = 10.01SFCDD8 pKa = 3.84FRR10 pKa = 11.84DD11 pKa = 3.45GRR13 pKa = 11.84RR14 pKa = 11.84QASAVRR20 pKa = 11.84KK21 pKa = 7.5CTSKK25 pKa = 10.76RR26 pKa = 11.84KK27 pKa = 9.01PLNTLTYY34 pKa = 8.92FGSRR38 pKa = 11.84AFRR41 pKa = 11.84PSFFRR46 pKa = 11.84VFKK49 pKa = 10.43PLIHH53 pKa = 6.06KK54 pKa = 9.76QIFRR58 pKa = 11.84KK59 pKa = 9.85SDD61 pKa = 3.29GNGFIYY67 pKa = 8.99KK68 pKa = 9.88TSFITDD74 pKa = 2.78NRR76 pKa = 11.84LPSRR80 pKa = 11.84TGEE83 pKa = 3.93KK84 pKa = 10.15KK85 pKa = 9.93KK86 pKa = 10.69ALRR89 pKa = 11.84CICAFLFFPLTYY101 pKa = 9.4EE102 pKa = 4.26GKK104 pKa = 9.5MNSS107 pKa = 3.21

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

14006

103

2328

304.5

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.031 ± 0.403

1.007 ± 0.202

6.419 ± 0.196

5.847 ± 0.382

3.534 ± 0.255

7.882 ± 0.354

1.514 ± 0.223

5.783 ± 0.208

5.462 ± 0.388

7.218 ± 0.332

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.208

5.126 ± 0.195

3.97 ± 0.348

4.655 ± 0.292

5.519 ± 0.238

5.448 ± 0.352

6.376 ± 0.559

6.547 ± 0.28

1.506 ± 0.151

3.306 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski