Pantoea sp. Eser

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea; unclassified Pantoea

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2328 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4VD28|A0A6I4VD28_9GAMM L D-transpeptidase OS=Pantoea sp. Eser OX=2576758 GN=ldtD PE=3 SV=1
MM1 pKa = 7.7AYY3 pKa = 9.17EE4 pKa = 4.49ALLDD8 pKa = 3.84EE9 pKa = 4.44QRR11 pKa = 11.84EE12 pKa = 4.2EE13 pKa = 3.7TRR15 pKa = 11.84LIIEE19 pKa = 4.54EE20 pKa = 4.2LLEE23 pKa = 5.25DD24 pKa = 4.98GSDD27 pKa = 3.55PDD29 pKa = 3.83ALYY32 pKa = 10.85TMEE35 pKa = 5.25HH36 pKa = 6.58HH37 pKa = 7.12LSCNNFDD44 pKa = 4.27SLEE47 pKa = 4.09KK48 pKa = 10.57AAVDD52 pKa = 3.66AFKK55 pKa = 10.84LGYY58 pKa = 9.93EE59 pKa = 4.03VTEE62 pKa = 4.16PEE64 pKa = 4.23EE65 pKa = 5.33LEE67 pKa = 5.14LEE69 pKa = 4.76DD70 pKa = 4.51GTTVMCVDD78 pKa = 5.1IISEE82 pKa = 4.28SALKK86 pKa = 10.51PEE88 pKa = 5.27LIDD91 pKa = 3.55VQVEE95 pKa = 3.97QLVNLIGKK103 pKa = 9.28YY104 pKa = 10.17NVDD107 pKa = 3.24YY108 pKa = 10.91DD109 pKa = 3.33GWGTYY114 pKa = 10.12FEE116 pKa = 5.93DD117 pKa = 5.6PDD119 pKa = 5.67AEE121 pKa = 4.45DD122 pKa = 4.67DD123 pKa = 3.75NGIRR127 pKa = 11.84HH128 pKa = 6.22

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4V884|A0A6I4V884_9GAMM Replicative DNA helicase OS=Pantoea sp. Eser OX=2576758 GN=dnaB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84TKK37 pKa = 10.23GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2328

0

2328

643241

15

1843

276.3

30.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.915 ± 0.054

1.061 ± 0.017

5.44 ± 0.034

5.816 ± 0.053

3.717 ± 0.037

7.276 ± 0.051

2.368 ± 0.028

5.578 ± 0.037

4.16 ± 0.046

10.879 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.023

3.571 ± 0.027

4.468 ± 0.036

4.909 ± 0.045

6.063 ± 0.045

5.888 ± 0.039

5.105 ± 0.033

7.064 ± 0.042

1.37 ± 0.02

2.634 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski