Geospiza fortis (Medium ground-finch)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8851 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I9HJP8|A0A6I9HJP8_GEOFO glomulin isoform X1 OS=Geospiza fortis OX=48883 GN=GLMN PE=4 SV=1
MM1 pKa = 7.52AVFHH5 pKa = 7.31DD6 pKa = 4.18EE7 pKa = 4.41VEE9 pKa = 4.39IEE11 pKa = 4.0DD12 pKa = 3.93FEE14 pKa = 4.88YY15 pKa = 11.07DD16 pKa = 4.03EE17 pKa = 4.37EE18 pKa = 4.7TGTYY22 pKa = 9.85SYY24 pKa = 10.48PCPCGDD30 pKa = 3.17RR31 pKa = 11.84FLITRR36 pKa = 11.84EE37 pKa = 3.86DD38 pKa = 3.83LEE40 pKa = 4.55NGEE43 pKa = 5.51DD44 pKa = 3.76VATCPSCSLILRR56 pKa = 11.84VIYY59 pKa = 10.21DD60 pKa = 3.18QEE62 pKa = 4.01QFMRR66 pKa = 11.84DD67 pKa = 3.32EE68 pKa = 4.55VVAEE72 pKa = 3.89PLPNKK77 pKa = 10.17EE78 pKa = 4.37LVKK81 pKa = 10.94CC82 pKa = 4.15

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I9HJK6|A0A6I9HJK6_GEOFO RBR-type E3 ubiquitin transferase OS=Geospiza fortis OX=48883 GN=RNF19A PE=4 SV=1
MM1 pKa = 7.17QPFAVVLAPRR11 pKa = 11.84DD12 pKa = 3.68PPIRR16 pKa = 11.84ACRR19 pKa = 11.84LPLSPSSPGAGSLLPGAAWPGKK41 pKa = 9.94EE42 pKa = 4.08PDD44 pKa = 3.65SSRR47 pKa = 11.84LAPGRR52 pKa = 11.84CRR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.71QSRR59 pKa = 11.84PAARR63 pKa = 11.84HH64 pKa = 4.72PWKK67 pKa = 10.3KK68 pKa = 10.74GNAAPDD74 pKa = 3.6SPRR77 pKa = 11.84TRR79 pKa = 11.84LARR82 pKa = 11.84SSWQGRR88 pKa = 11.84SAAPAMPHH96 pKa = 5.46FLDD99 pKa = 3.52WFVPVYY105 pKa = 10.92LMISILILVGFGACIYY121 pKa = 9.97YY122 pKa = 9.49FEE124 pKa = 5.98PGLQEE129 pKa = 3.54AHH131 pKa = 6.58KK132 pKa = 10.06WRR134 pKa = 11.84TQRR137 pKa = 11.84PIMEE141 pKa = 4.88RR142 pKa = 11.84DD143 pKa = 3.18LRR145 pKa = 11.84KK146 pKa = 8.34TLMIRR151 pKa = 11.84DD152 pKa = 3.79NLAFGVPEE160 pKa = 4.03VV161 pKa = 3.73

Molecular weight: 17.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8284

567

8851

4549436

44

5048

514.0

57.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.021

2.245 ± 0.021

5.003 ± 0.017

7.195 ± 0.032

3.902 ± 0.017

6.01 ± 0.03

2.543 ± 0.012

4.889 ± 0.018

6.282 ± 0.028

9.73 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.365 ± 0.012

4.039 ± 0.017

5.35 ± 0.029

4.667 ± 0.021

5.213 ± 0.018

8.219 ± 0.035

5.297 ± 0.019

6.219 ± 0.021

1.199 ± 0.008

2.947 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski