Palaemonetes intermedius brackish grass shrimp associated circular virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1RL48|A0A0K1RL48_9CIRC Putative capsid protein OS=Palaemonetes intermedius brackish grass shrimp associated circular virus OX=1692257 PE=4 SV=1
MM1 pKa = 7.85NIRR4 pKa = 11.84PAAVKK9 pKa = 10.14RR10 pKa = 11.84WVATVNNPTQQEE22 pKa = 4.46SQTLRR27 pKa = 11.84DD28 pKa = 4.08AIEE31 pKa = 4.24AQTSYY36 pKa = 11.44AVIGRR41 pKa = 11.84EE42 pKa = 4.09VGEE45 pKa = 4.37SGTPHH50 pKa = 6.6LQCFFIFNNRR60 pKa = 11.84LRR62 pKa = 11.84LRR64 pKa = 11.84QVKK67 pKa = 9.48AVPGLQRR74 pKa = 11.84AHH76 pKa = 7.06LEE78 pKa = 4.03PARR81 pKa = 11.84GTSAQAANYY90 pKa = 8.03CKK92 pKa = 10.15KK93 pKa = 10.76DD94 pKa = 3.21GDD96 pKa = 3.55FDD98 pKa = 4.57EE99 pKa = 5.52YY100 pKa = 11.78GEE102 pKa = 4.87LPNSGPKK109 pKa = 7.81TTIFEE114 pKa = 4.81AFRR117 pKa = 11.84DD118 pKa = 3.88WYY120 pKa = 10.38KK121 pKa = 10.82DD122 pKa = 3.22QPGVVTEE129 pKa = 4.22RR130 pKa = 11.84DD131 pKa = 3.5ILDD134 pKa = 3.45HH135 pKa = 6.69HH136 pKa = 6.83PSILRR141 pKa = 11.84YY142 pKa = 7.61PHH144 pKa = 6.86FIEE147 pKa = 4.52VCHH150 pKa = 5.76RR151 pKa = 11.84QYY153 pKa = 11.43GRR155 pKa = 11.84RR156 pKa = 11.84PTLVEE161 pKa = 3.76GQLRR165 pKa = 11.84NWQLEE170 pKa = 4.24LSNMLDD176 pKa = 3.62EE177 pKa = 5.22EE178 pKa = 5.02PDD180 pKa = 3.37DD181 pKa = 4.77RR182 pKa = 11.84KK183 pKa = 10.48ICFVVDD189 pKa = 3.24EE190 pKa = 5.05EE191 pKa = 4.76GNKK194 pKa = 9.51GKK196 pKa = 10.19SWLTAYY202 pKa = 9.03WYY204 pKa = 10.83SNRR207 pKa = 11.84SDD209 pKa = 3.43VQMLSIGKK217 pKa = 9.51RR218 pKa = 11.84DD219 pKa = 3.95DD220 pKa = 3.06LTYY223 pKa = 10.75AIDD226 pKa = 3.34VSKK229 pKa = 10.75RR230 pKa = 11.84VFVFDD235 pKa = 3.63INRR238 pKa = 11.84GQMEE242 pKa = 4.4YY243 pKa = 10.24FQYY246 pKa = 11.22SVVEE250 pKa = 4.06SLKK253 pKa = 10.81NRR255 pKa = 11.84MIMSNKK261 pKa = 8.66YY262 pKa = 10.2KK263 pKa = 10.47SVTKK267 pKa = 10.45IIPHH271 pKa = 5.99KK272 pKa = 8.39VHH274 pKa = 6.8VIVFCNEE281 pKa = 3.73EE282 pKa = 3.97PDD284 pKa = 3.58RR285 pKa = 11.84TAMTRR290 pKa = 11.84DD291 pKa = 3.32RR292 pKa = 11.84YY293 pKa = 8.68QMKK296 pKa = 10.54RR297 pKa = 11.84ITPLL301 pKa = 3.16

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1RL48|A0A0K1RL48_9CIRC Putative capsid protein OS=Palaemonetes intermedius brackish grass shrimp associated circular virus OX=1692257 PE=4 SV=1
MM1 pKa = 7.03QAGLAALAEE10 pKa = 4.25GDD12 pKa = 4.17YY13 pKa = 10.35IYY15 pKa = 10.74PLAQYY20 pKa = 11.07AGLEE24 pKa = 4.17LLASAAARR32 pKa = 11.84HH33 pKa = 5.49GSNRR37 pKa = 11.84GTYY40 pKa = 8.5MPAEE44 pKa = 3.96SFLRR48 pKa = 11.84KK49 pKa = 9.17RR50 pKa = 11.84AAVTPDD56 pKa = 2.96EE57 pKa = 4.78PQTKK61 pKa = 9.49RR62 pKa = 11.84QRR64 pKa = 11.84TYY66 pKa = 8.8TYY68 pKa = 10.68APRR71 pKa = 11.84VLNMPAGVMTRR82 pKa = 11.84TRR84 pKa = 11.84RR85 pKa = 11.84TVYY88 pKa = 10.04KK89 pKa = 9.84YY90 pKa = 11.07NLGTRR95 pKa = 11.84VGKK98 pKa = 8.88FKK100 pKa = 11.05CNKK103 pKa = 9.05FSFKK107 pKa = 10.56TGVTNNMADD116 pKa = 3.53KK117 pKa = 10.22AKK119 pKa = 10.61HH120 pKa = 4.9SFRR123 pKa = 11.84LIQIPYY129 pKa = 10.18SSDD132 pKa = 3.15DD133 pKa = 3.4SLFNTRR139 pKa = 11.84NGRR142 pKa = 11.84LVNVRR147 pKa = 11.84GVRR150 pKa = 11.84LQWVCEE156 pKa = 3.97LKK158 pKa = 10.84PGGSTGNIEE167 pKa = 4.2RR168 pKa = 11.84PLSVRR173 pKa = 11.84WAIINPKK180 pKa = 9.74EE181 pKa = 3.93NDD183 pKa = 3.48GTSTVGTANWWTSLNPTNEE202 pKa = 3.94YY203 pKa = 9.67ATDD206 pKa = 4.31FVNGSGSTTPGTGTGYY222 pKa = 10.71QDD224 pKa = 3.28WFDD227 pKa = 4.04LMTRR231 pKa = 11.84GINRR235 pKa = 11.84RR236 pKa = 11.84KK237 pKa = 10.35YY238 pKa = 8.67GVLKK242 pKa = 10.14EE243 pKa = 4.0GHH245 pKa = 5.42FTISPPEE252 pKa = 3.94TSDD255 pKa = 3.13ADD257 pKa = 3.67SRR259 pKa = 11.84KK260 pKa = 7.12TWRR263 pKa = 11.84QQKK266 pKa = 10.06VIQTYY271 pKa = 10.71LKK273 pKa = 10.37INQCMKK279 pKa = 9.69WGSNNTGGEE288 pKa = 3.86NDD290 pKa = 3.71YY291 pKa = 11.34PNANLYY297 pKa = 8.01FVWWYY302 pKa = 10.63CDD304 pKa = 3.23RR305 pKa = 11.84GNMDD309 pKa = 4.3DD310 pKa = 4.19LQWYY314 pKa = 8.47PSVNYY319 pKa = 10.07VPLQTQAQVTVFFKK333 pKa = 10.02TAKK336 pKa = 10.43GLLL339 pKa = 3.75

Molecular weight: 38.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

640

301

339

320.0

36.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.25 ± 0.63

1.406 ± 0.172

5.313 ± 0.674

4.844 ± 1.214

3.75 ± 0.16

6.875 ± 1.275

1.719 ± 0.633

4.219 ± 0.963

5.625 ± 0.015

6.719 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.106

6.25 ± 0.854

5.0 ± 0.011

4.844 ± 0.318

7.813 ± 0.556

5.469 ± 0.103

7.5 ± 1.473

6.719 ± 0.622

2.344 ± 0.46

4.844 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski