Momordica charantia (Bitter gourd) (Balsam pear)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Momordiceae; Momordica

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1DIT2|A0A6J1DIT2_MOMCH Lipase OS=Momordica charantia OX=3673 GN=LOC111021471 PE=3 SV=1
MM1 pKa = 7.61ALASVEE7 pKa = 4.16GRR9 pKa = 11.84STLNPNAPLFIPAAYY24 pKa = 9.38QVEE27 pKa = 4.54DD28 pKa = 5.92FSPQWWQLVTTSTWYY43 pKa = 9.91RR44 pKa = 11.84DD45 pKa = 3.4YY46 pKa = 10.77WLSQHH51 pKa = 6.04QEE53 pKa = 3.55EE54 pKa = 4.56SDD56 pKa = 4.14FYY58 pKa = 11.17IEE60 pKa = 6.48DD61 pKa = 4.23DD62 pKa = 4.25FNSNDD67 pKa = 3.46IADD70 pKa = 5.14LLPEE74 pKa = 5.15AFDD77 pKa = 4.31LDD79 pKa = 4.03ANEE82 pKa = 4.68EE83 pKa = 4.19LRR85 pKa = 11.84TMEE88 pKa = 4.62AEE90 pKa = 3.88FEE92 pKa = 4.21EE93 pKa = 5.53FIQASLTEE101 pKa = 4.05AHH103 pKa = 6.58HH104 pKa = 7.1PEE106 pKa = 4.0MM107 pKa = 5.79

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1DPD2|A0A6J1DPD2_MOMCH uncharacterized protein LOC111023073 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023073 PE=4 SV=1
MM1 pKa = 7.81PNLPDD6 pKa = 3.06IMGNVASSVASGFFSAVGKK25 pKa = 9.77LFRR28 pKa = 11.84SPLDD32 pKa = 3.5FLSGKK37 pKa = 9.74SCSSVCGSTWDD48 pKa = 4.39FICYY52 pKa = 9.68IEE54 pKa = 4.35NFCVANLLKK63 pKa = 10.8LGMVLILSLFVILLLYY79 pKa = 10.19LLHH82 pKa = 7.15KK83 pKa = 10.38IGIFGCICRR92 pKa = 11.84GLCRR96 pKa = 11.84MTWTCIASYY105 pKa = 9.68FYY107 pKa = 11.19AWDD110 pKa = 3.7YY111 pKa = 11.29CCTFMCIKK119 pKa = 10.15LGSVKK124 pKa = 9.02RR125 pKa = 11.84TRR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84HH131 pKa = 4.99RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84DD135 pKa = 3.35LEE137 pKa = 4.2EE138 pKa = 4.02EE139 pKa = 4.27FEE141 pKa = 4.58SEE143 pKa = 4.26GGKK146 pKa = 9.29HH147 pKa = 6.01RR148 pKa = 11.84YY149 pKa = 9.2GSSSDD154 pKa = 3.33SSSVPEE160 pKa = 4.39RR161 pKa = 11.84IEE163 pKa = 3.94LRR165 pKa = 11.84SSQRR169 pKa = 11.84ASRR172 pKa = 11.84RR173 pKa = 11.84WRR175 pKa = 11.84MNHH178 pKa = 6.8RR179 pKa = 11.84GSQMRR184 pKa = 11.84KK185 pKa = 8.57ALRR188 pKa = 11.84PKK190 pKa = 10.26SRR192 pKa = 11.84GIRR195 pKa = 11.84VRR197 pKa = 11.84SGRR200 pKa = 11.84TLVYY204 pKa = 9.95GKK206 pKa = 9.61HH207 pKa = 5.27RR208 pKa = 11.84RR209 pKa = 11.84KK210 pKa = 10.27SSEE213 pKa = 3.71VVNRR217 pKa = 11.84LGEE220 pKa = 3.91IHH222 pKa = 6.71SLGRR226 pKa = 11.84HH227 pKa = 4.9GSSKK231 pKa = 10.07FVHH234 pKa = 6.09EE235 pKa = 4.69EE236 pKa = 3.06IRR238 pKa = 11.84YY239 pKa = 9.21KK240 pKa = 10.57RR241 pKa = 11.84GRR243 pKa = 11.84QKK245 pKa = 11.28

Molecular weight: 28.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19393

4647

24040

11072412

25

5429

460.6

51.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.817 ± 0.013

1.861 ± 0.007

5.336 ± 0.01

6.552 ± 0.017

4.262 ± 0.01

6.463 ± 0.013

2.398 ± 0.006

5.39 ± 0.009

6.034 ± 0.015

9.739 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.006

4.438 ± 0.009

4.835 ± 0.013

3.664 ± 0.01

5.462 ± 0.011

9.186 ± 0.019

4.716 ± 0.009

6.448 ± 0.011

1.264 ± 0.005

2.743 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski