Amino acid dipepetide frequency for Mycobacterium phage Gail

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.335AlaAla: 12.335 ± 1.152
0.361AlaCys: 0.361 ± 0.121
5.114AlaAsp: 5.114 ± 0.497
6.318AlaGlu: 6.318 ± 0.791
3.008AlaPhe: 3.008 ± 0.366
7.28AlaGly: 7.28 ± 0.969
1.625AlaHis: 1.625 ± 0.387
5.114AlaIle: 5.114 ± 0.576
4.633AlaLys: 4.633 ± 0.614
9.025AlaLeu: 9.025 ± 0.972
2.888AlaMet: 2.888 ± 0.342
3.309AlaAsn: 3.309 ± 0.545
4.693AlaPro: 4.693 ± 0.573
3.249AlaGln: 3.249 ± 0.486
6.197AlaArg: 6.197 ± 0.646
5.174AlaSer: 5.174 ± 0.483
5.355AlaThr: 5.355 ± 0.693
7.28AlaVal: 7.28 ± 0.635
1.986AlaTrp: 1.986 ± 0.41
2.888AlaTyr: 2.888 ± 0.42
0.0AlaXaa: 0.0 ± 0.0
Cys
0.421CysAla: 0.421 ± 0.151
0.0CysCys: 0.0 ± 0.0
0.542CysAsp: 0.542 ± 0.162
0.662CysGlu: 0.662 ± 0.197
0.301CysPhe: 0.301 ± 0.136
1.143CysGly: 1.143 ± 0.247
0.301CysHis: 0.301 ± 0.146
0.602CysIle: 0.602 ± 0.232
0.361CysLys: 0.361 ± 0.156
0.662CysLeu: 0.662 ± 0.181
0.0CysMet: 0.0 ± 0.0
0.602CysAsn: 0.602 ± 0.176
0.421CysPro: 0.421 ± 0.167
0.181CysGln: 0.181 ± 0.096
0.602CysArg: 0.602 ± 0.209
0.421CysSer: 0.421 ± 0.174
0.602CysThr: 0.602 ± 0.174
0.481CysVal: 0.481 ± 0.132
0.241CysTrp: 0.241 ± 0.148
0.301CysTyr: 0.301 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
6.498AspAla: 6.498 ± 0.721
0.542AspCys: 0.542 ± 0.194
3.73AspAsp: 3.73 ± 0.545
4.031AspGlu: 4.031 ± 0.514
3.069AspPhe: 3.069 ± 0.392
6.679AspGly: 6.679 ± 0.625
1.745AspHis: 1.745 ± 0.372
3.791AspIle: 3.791 ± 0.566
2.226AspLys: 2.226 ± 0.364
5.656AspLeu: 5.656 ± 0.564
1.444AspMet: 1.444 ± 0.269
2.166AspAsn: 2.166 ± 0.369
4.934AspPro: 4.934 ± 0.617
2.647AspGln: 2.647 ± 0.395
3.129AspArg: 3.129 ± 0.377
3.008AspSer: 3.008 ± 0.457
3.55AspThr: 3.55 ± 0.419
4.813AspVal: 4.813 ± 0.504
1.444AspTrp: 1.444 ± 0.37
2.347AspTyr: 2.347 ± 0.365
0.0AspXaa: 0.0 ± 0.0
Glu
7.341GluAla: 7.341 ± 0.82
0.421GluCys: 0.421 ± 0.15
3.911GluAsp: 3.911 ± 0.467
5.054GluGlu: 5.054 ± 0.617
2.647GluPhe: 2.647 ± 0.395
5.235GluGly: 5.235 ± 0.55
1.986GluHis: 1.986 ± 0.36
3.73GluIle: 3.73 ± 0.467
2.407GluLys: 2.407 ± 0.473
6.137GluLeu: 6.137 ± 0.681
2.046GluMet: 2.046 ± 0.292
2.226GluAsn: 2.226 ± 0.394
3.55GluPro: 3.55 ± 0.456
1.805GluGln: 1.805 ± 0.26
4.272GluArg: 4.272 ± 0.535
2.888GluSer: 2.888 ± 0.406
3.55GluThr: 3.55 ± 0.43
4.573GluVal: 4.573 ± 0.479
1.143GluTrp: 1.143 ± 0.27
2.046GluTyr: 2.046 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
2.768PheAla: 2.768 ± 0.452
0.421PheCys: 0.421 ± 0.145
2.768PheAsp: 2.768 ± 0.527
2.768PheGlu: 2.768 ± 0.431
0.782PhePhe: 0.782 ± 0.249
3.129PheGly: 3.129 ± 0.38
0.602PheHis: 0.602 ± 0.228
1.264PheIle: 1.264 ± 0.275
1.504PheLys: 1.504 ± 0.311
2.587PheLeu: 2.587 ± 0.386
0.602PheMet: 0.602 ± 0.211
1.504PheAsn: 1.504 ± 0.315
1.504PhePro: 1.504 ± 0.317
0.963PheGln: 0.963 ± 0.248
2.647PheArg: 2.647 ± 0.32
2.708PheSer: 2.708 ± 0.458
1.925PheThr: 1.925 ± 0.422
2.647PheVal: 2.647 ± 0.399
0.481PheTrp: 0.481 ± 0.186
0.963PheTyr: 0.963 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
7.28GlyAla: 7.28 ± 0.831
0.722GlyCys: 0.722 ± 0.253
5.475GlyAsp: 5.475 ± 0.672
4.693GlyGlu: 4.693 ± 0.621
2.708GlyPhe: 2.708 ± 0.384
9.206GlyGly: 9.206 ± 1.686
1.625GlyHis: 1.625 ± 0.377
4.152GlyIle: 4.152 ± 0.659
4.874GlyLys: 4.874 ± 0.525
7.28GlyLeu: 7.28 ± 0.677
2.166GlyMet: 2.166 ± 0.332
2.768GlyAsn: 2.768 ± 0.406
3.189GlyPro: 3.189 ± 0.399
2.407GlyGln: 2.407 ± 0.382
3.61GlyArg: 3.61 ± 0.476
4.513GlySer: 4.513 ± 0.539
5.475GlyThr: 5.475 ± 0.75
6.077GlyVal: 6.077 ± 0.622
1.986GlyTrp: 1.986 ± 0.346
2.226GlyTyr: 2.226 ± 0.333
0.0GlyXaa: 0.0 ± 0.0
His
1.625HisAla: 1.625 ± 0.318
0.12HisCys: 0.12 ± 0.087
1.745HisAsp: 1.745 ± 0.359
1.264HisGlu: 1.264 ± 0.25
0.602HisPhe: 0.602 ± 0.175
1.745HisGly: 1.745 ± 0.42
0.722HisHis: 0.722 ± 0.212
1.083HisIle: 1.083 ± 0.251
1.023HisLys: 1.023 ± 0.256
1.083HisLeu: 1.083 ± 0.306
0.301HisMet: 0.301 ± 0.146
0.421HisAsn: 0.421 ± 0.162
0.842HisPro: 0.842 ± 0.182
0.903HisGln: 0.903 ± 0.189
1.264HisArg: 1.264 ± 0.275
1.023HisSer: 1.023 ± 0.23
1.504HisThr: 1.504 ± 0.282
1.625HisVal: 1.625 ± 0.268
0.361HisTrp: 0.361 ± 0.169
0.722HisTyr: 0.722 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
4.934IleAla: 4.934 ± 0.608
0.662IleCys: 0.662 ± 0.167
5.475IleAsp: 5.475 ± 0.567
4.573IleGlu: 4.573 ± 0.516
1.384IlePhe: 1.384 ± 0.317
4.513IleGly: 4.513 ± 0.575
0.602IleHis: 0.602 ± 0.173
2.046IleIle: 2.046 ± 0.384
2.888IleLys: 2.888 ± 0.411
3.008IleLeu: 3.008 ± 0.519
0.542IleMet: 0.542 ± 0.185
2.046IleAsn: 2.046 ± 0.412
3.43IlePro: 3.43 ± 0.364
1.745IleGln: 1.745 ± 0.325
3.55IleArg: 3.55 ± 0.438
1.925IleSer: 1.925 ± 0.381
3.43IleThr: 3.43 ± 0.45
2.647IleVal: 2.647 ± 0.34
1.083IleTrp: 1.083 ± 0.318
0.903IleTyr: 0.903 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
4.753LysAla: 4.753 ± 0.505
0.181LysCys: 0.181 ± 0.092
2.347LysAsp: 2.347 ± 0.475
3.43LysGlu: 3.43 ± 0.656
1.504LysPhe: 1.504 ± 0.247
3.49LysGly: 3.49 ± 0.454
0.782LysHis: 0.782 ± 0.217
3.309LysIle: 3.309 ± 0.518
2.407LysLys: 2.407 ± 0.516
4.031LysLeu: 4.031 ± 0.344
1.504LysMet: 1.504 ± 0.283
1.564LysAsn: 1.564 ± 0.349
3.008LysPro: 3.008 ± 0.519
1.925LysGln: 1.925 ± 0.301
3.911LysArg: 3.911 ± 0.508
2.587LysSer: 2.587 ± 0.347
2.407LysThr: 2.407 ± 0.458
3.61LysVal: 3.61 ± 0.522
1.143LysTrp: 1.143 ± 0.239
1.023LysTyr: 1.023 ± 0.211
0.0LysXaa: 0.0 ± 0.0
Leu
7.461LeuAla: 7.461 ± 0.613
0.662LeuCys: 0.662 ± 0.175
5.355LeuAsp: 5.355 ± 0.707
5.535LeuGlu: 5.535 ± 0.583
3.189LeuPhe: 3.189 ± 0.478
5.235LeuGly: 5.235 ± 0.502
1.986LeuHis: 1.986 ± 0.386
3.61LeuIle: 3.61 ± 0.466
4.031LeuLys: 4.031 ± 0.527
5.716LeuLeu: 5.716 ± 0.575
2.407LeuMet: 2.407 ± 0.372
2.587LeuAsn: 2.587 ± 0.457
5.114LeuPro: 5.114 ± 0.441
1.986LeuGln: 1.986 ± 0.414
4.994LeuArg: 4.994 ± 0.697
4.994LeuSer: 4.994 ± 0.526
5.114LeuThr: 5.114 ± 0.639
5.656LeuVal: 5.656 ± 0.702
1.685LeuTrp: 1.685 ± 0.326
1.805LeuTyr: 1.805 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
3.129MetAla: 3.129 ± 0.392
0.181MetCys: 0.181 ± 0.102
1.083MetAsp: 1.083 ± 0.262
1.324MetGlu: 1.324 ± 0.265
0.722MetPhe: 0.722 ± 0.191
1.564MetGly: 1.564 ± 0.295
0.361MetHis: 0.361 ± 0.124
1.143MetIle: 1.143 ± 0.318
1.203MetLys: 1.203 ± 0.284
1.203MetLeu: 1.203 ± 0.301
0.421MetMet: 0.421 ± 0.187
0.782MetAsn: 0.782 ± 0.192
1.384MetPro: 1.384 ± 0.311
0.782MetGln: 0.782 ± 0.192
1.625MetArg: 1.625 ± 0.283
2.828MetSer: 2.828 ± 0.431
2.286MetThr: 2.286 ± 0.348
1.264MetVal: 1.264 ± 0.289
0.361MetTrp: 0.361 ± 0.147
0.481MetTyr: 0.481 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
3.43AsnAla: 3.43 ± 0.416
0.241AsnCys: 0.241 ± 0.103
1.925AsnAsp: 1.925 ± 0.304
2.046AsnGlu: 2.046 ± 0.388
1.203AsnPhe: 1.203 ± 0.259
3.129AsnGly: 3.129 ± 0.478
0.782AsnHis: 0.782 ± 0.222
1.685AsnIle: 1.685 ± 0.277
1.264AsnLys: 1.264 ± 0.252
2.888AsnLeu: 2.888 ± 0.469
0.963AsnMet: 0.963 ± 0.23
0.963AsnAsn: 0.963 ± 0.306
2.286AsnPro: 2.286 ± 0.308
1.023AsnGln: 1.023 ± 0.212
2.527AsnArg: 2.527 ± 0.453
1.685AsnSer: 1.685 ± 0.258
1.625AsnThr: 1.625 ± 0.29
2.587AsnVal: 2.587 ± 0.353
0.963AsnTrp: 0.963 ± 0.251
0.903AsnTyr: 0.903 ± 0.23
0.0AsnXaa: 0.0 ± 0.0
Pro
4.693ProAla: 4.693 ± 0.566
0.481ProCys: 0.481 ± 0.201
4.091ProAsp: 4.091 ± 0.465
3.73ProGlu: 3.73 ± 0.491
1.745ProPhe: 1.745 ± 0.292
4.573ProGly: 4.573 ± 0.547
0.842ProHis: 0.842 ± 0.242
2.647ProIle: 2.647 ± 0.342
2.828ProLys: 2.828 ± 0.437
3.43ProLeu: 3.43 ± 0.501
0.903ProMet: 0.903 ± 0.224
1.865ProAsn: 1.865 ± 0.239
1.865ProPro: 1.865 ± 0.343
1.805ProGln: 1.805 ± 0.286
2.948ProArg: 2.948 ± 0.472
3.129ProSer: 3.129 ± 0.419
4.031ProThr: 4.031 ± 0.468
3.61ProVal: 3.61 ± 0.502
0.903ProTrp: 0.903 ± 0.278
1.504ProTyr: 1.504 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
4.212GlnAla: 4.212 ± 0.531
0.241GlnCys: 0.241 ± 0.113
1.925GlnAsp: 1.925 ± 0.392
1.564GlnGlu: 1.564 ± 0.339
1.745GlnPhe: 1.745 ± 0.348
2.527GlnGly: 2.527 ± 0.359
0.662GlnHis: 0.662 ± 0.176
2.828GlnIle: 2.828 ± 0.419
1.865GlnLys: 1.865 ± 0.285
2.347GlnLeu: 2.347 ± 0.416
0.842GlnMet: 0.842 ± 0.201
0.662GlnAsn: 0.662 ± 0.208
1.384GlnPro: 1.384 ± 0.35
1.384GlnGln: 1.384 ± 0.366
2.106GlnArg: 2.106 ± 0.331
1.143GlnSer: 1.143 ± 0.252
1.745GlnThr: 1.745 ± 0.315
2.286GlnVal: 2.286 ± 0.312
1.023GlnTrp: 1.023 ± 0.265
0.842GlnTyr: 0.842 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
5.475ArgAla: 5.475 ± 0.721
1.444ArgCys: 1.444 ± 0.345
4.152ArgAsp: 4.152 ± 0.484
4.573ArgGlu: 4.573 ± 0.615
2.046ArgPhe: 2.046 ± 0.413
3.67ArgGly: 3.67 ± 0.466
1.264ArgHis: 1.264 ± 0.242
3.309ArgIle: 3.309 ± 0.435
3.309ArgLys: 3.309 ± 0.514
5.596ArgLeu: 5.596 ± 0.711
1.564ArgMet: 1.564 ± 0.303
1.805ArgAsn: 1.805 ± 0.303
2.347ArgPro: 2.347 ± 0.342
2.587ArgGln: 2.587 ± 0.522
5.716ArgArg: 5.716 ± 0.64
2.467ArgSer: 2.467 ± 0.348
3.129ArgThr: 3.129 ± 0.377
4.934ArgVal: 4.934 ± 0.52
1.023ArgTrp: 1.023 ± 0.268
2.708ArgTyr: 2.708 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
5.114SerAla: 5.114 ± 0.456
0.421SerCys: 0.421 ± 0.148
3.911SerAsp: 3.911 ± 0.545
4.091SerGlu: 4.091 ± 0.432
1.865SerPhe: 1.865 ± 0.36
4.633SerGly: 4.633 ± 0.691
0.842SerHis: 0.842 ± 0.197
2.467SerIle: 2.467 ± 0.389
3.249SerLys: 3.249 ± 0.436
3.791SerLeu: 3.791 ± 0.587
1.264SerMet: 1.264 ± 0.233
1.805SerAsn: 1.805 ± 0.334
3.189SerPro: 3.189 ± 0.383
1.384SerGln: 1.384 ± 0.282
3.55SerArg: 3.55 ± 0.434
2.587SerSer: 2.587 ± 0.387
4.031SerThr: 4.031 ± 0.51
3.61SerVal: 3.61 ± 0.417
0.842SerTrp: 0.842 ± 0.21
1.444SerTyr: 1.444 ± 0.263
0.0SerXaa: 0.0 ± 0.0
Thr
5.596ThrAla: 5.596 ± 0.552
0.481ThrCys: 0.481 ± 0.161
4.272ThrAsp: 4.272 ± 0.627
3.249ThrGlu: 3.249 ± 0.412
1.986ThrPhe: 1.986 ± 0.316
5.957ThrGly: 5.957 ± 0.673
1.324ThrHis: 1.324 ± 0.262
2.948ThrIle: 2.948 ± 0.408
3.129ThrLys: 3.129 ± 0.436
5.054ThrLeu: 5.054 ± 0.469
1.384ThrMet: 1.384 ± 0.246
2.166ThrAsn: 2.166 ± 0.336
2.948ThrPro: 2.948 ± 0.475
2.286ThrGln: 2.286 ± 0.366
3.189ThrArg: 3.189 ± 0.473
2.708ThrSer: 2.708 ± 0.421
3.911ThrThr: 3.911 ± 0.501
5.054ThrVal: 5.054 ± 0.594
1.504ThrTrp: 1.504 ± 0.323
2.046ThrTyr: 2.046 ± 0.348
0.0ThrXaa: 0.0 ± 0.0
Val
5.957ValAla: 5.957 ± 0.651
0.782ValCys: 0.782 ± 0.227
5.776ValAsp: 5.776 ± 0.642
4.874ValGlu: 4.874 ± 0.488
2.647ValPhe: 2.647 ± 0.38
4.813ValGly: 4.813 ± 0.548
1.384ValHis: 1.384 ± 0.253
2.948ValIle: 2.948 ± 0.437
3.73ValLys: 3.73 ± 0.408
5.415ValLeu: 5.415 ± 0.618
1.865ValMet: 1.865 ± 0.349
3.129ValAsn: 3.129 ± 0.513
2.708ValPro: 2.708 ± 0.345
2.046ValGln: 2.046 ± 0.366
4.152ValArg: 4.152 ± 0.514
4.813ValSer: 4.813 ± 0.576
4.753ValThr: 4.753 ± 0.641
4.753ValVal: 4.753 ± 0.597
1.986ValTrp: 1.986 ± 0.326
2.286ValTyr: 2.286 ± 0.393
0.0ValXaa: 0.0 ± 0.0
Trp
2.106TrpAla: 2.106 ± 0.356
0.421TrpCys: 0.421 ± 0.165
2.046TrpAsp: 2.046 ± 0.352
1.264TrpGlu: 1.264 ± 0.261
0.662TrpPhe: 0.662 ± 0.218
1.564TrpGly: 1.564 ± 0.311
0.301TrpHis: 0.301 ± 0.122
1.384TrpIle: 1.384 ± 0.292
1.083TrpLys: 1.083 ± 0.279
1.264TrpLeu: 1.264 ± 0.347
0.301TrpMet: 0.301 ± 0.131
1.083TrpAsn: 1.083 ± 0.414
0.963TrpPro: 0.963 ± 0.303
1.083TrpGln: 1.083 ± 0.249
1.023TrpArg: 1.023 ± 0.223
1.805TrpSer: 1.805 ± 0.381
1.023TrpThr: 1.023 ± 0.251
1.203TrpVal: 1.203 ± 0.245
0.421TrpTrp: 0.421 ± 0.145
0.481TrpTyr: 0.481 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.708TyrAla: 2.708 ± 0.334
0.06TyrCys: 0.06 ± 0.06
1.805TyrAsp: 1.805 ± 0.353
1.925TyrGlu: 1.925 ± 0.412
0.722TyrPhe: 0.722 ± 0.19
2.347TyrGly: 2.347 ± 0.381
0.241TyrHis: 0.241 ± 0.133
1.324TyrIle: 1.324 ± 0.257
0.903TyrLys: 0.903 ± 0.221
2.948TyrLeu: 2.948 ± 0.389
0.722TyrMet: 0.722 ± 0.216
0.662TyrAsn: 0.662 ± 0.159
1.865TyrPro: 1.865 ± 0.331
1.143TyrGln: 1.143 ± 0.243
2.106TyrArg: 2.106 ± 0.402
1.685TyrSer: 1.685 ± 0.3
1.745TyrThr: 1.745 ± 0.271
2.106TyrVal: 2.106 ± 0.367
0.903TyrTrp: 0.903 ± 0.24
0.722TyrTyr: 0.722 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (16621 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski