Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Janthinobacterium; Janthinobacterium agaricidamnosum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5488 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0V944|W0V944_9BURK CRISPR-associated endonuclease Cas1 OS=Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628 OX=1349767 GN=cas1 PE=3 SV=1
MM1 pKa = 6.86XXQAATAADD10 pKa = 3.61AQAAASAAQAAISLAALHH28 pKa = 6.78AAQADD33 pKa = 3.94DD34 pKa = 3.87AAAAAAAAFAAQAQANAQQAHH55 pKa = 6.95ADD57 pKa = 3.93DD58 pKa = 4.82ASAAAAQAAADD69 pKa = 3.85AAQVALAQANAIAAQTDD86 pKa = 3.39AHH88 pKa = 6.57ALGLISDD95 pKa = 4.67ADD97 pKa = 3.78ALAAAAAQAIADD109 pKa = 3.95QTDD112 pKa = 3.18AAALAQQAAAHH123 pKa = 6.35AAAAAAAQATADD135 pKa = 3.94LTDD138 pKa = 3.64AQALGLVADD147 pKa = 4.87AAHH150 pKa = 6.92AAAIDD155 pKa = 3.78ASNQADD161 pKa = 4.1LTDD164 pKa = 3.82AAALAQIASHH174 pKa = 7.05DD175 pKa = 3.76AAAALAAQQLADD187 pKa = 3.89LTDD190 pKa = 4.09ANHH193 pKa = 7.24LLTISTLAHH202 pKa = 6.52NSPQIVTGFQGGALADD218 pKa = 4.11GGDD221 pKa = 4.11VIDD224 pKa = 5.85LSALGHH230 pKa = 6.62LGSLSLAAGVNLHH243 pKa = 6.29TDD245 pKa = 4.16FGADD249 pKa = 3.0NFFVFDD255 pKa = 4.09DD256 pKa = 4.32TPISIHH262 pKa = 6.44DD263 pKa = 5.09AISAIAADD271 pKa = 3.97SSVLSGQGYY280 pKa = 9.7IVIHH284 pKa = 6.79DD285 pKa = 4.0AANNGAVTLYY295 pKa = 10.44HH296 pKa = 7.05SSDD299 pKa = 3.39LSSATGTEE307 pKa = 3.98TALVLLSGLTTTQHH321 pKa = 5.64LTQANFIVV329 pKa = 3.64

Molecular weight: 31.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0VDK5|W0VDK5_9BURK NUDIX domain protein OS=Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628 OX=1349767 GN=GJA_4844 PE=4 SV=1
MM1 pKa = 7.13TRR3 pKa = 11.84QLARR7 pKa = 11.84RR8 pKa = 11.84LAKK11 pKa = 9.85VWPRR15 pKa = 11.84QAWLQPMQVLISSVRR30 pKa = 11.84PLAALLTNSGSARR43 pKa = 11.84NGRR46 pKa = 11.84AIDD49 pKa = 3.62TRR51 pKa = 11.84SASPRR56 pKa = 11.84SMMM59 pKa = 4.79

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5488

0

5488

1754291

37

5090

319.7

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.432 ± 0.057

0.879 ± 0.012

5.429 ± 0.023

4.77 ± 0.03

3.579 ± 0.024

8.142 ± 0.04

2.265 ± 0.019

4.975 ± 0.025

3.68 ± 0.029

10.897 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.019

3.21 ± 0.03

4.913 ± 0.029

4.369 ± 0.025

6.169 ± 0.034

6.01 ± 0.033

4.988 ± 0.032

6.952 ± 0.029

1.309 ± 0.012

2.566 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski