Peptostreptococcaceae bacterium AS15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; unclassified Peptostreptococcaceae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J4UEC3|J4UEC3_9FIRM 33 kDa chaperonin OS=Peptostreptococcaceae bacterium AS15 OX=936556 GN=hslO PE=3 SV=1
MM1 pKa = 7.37YY2 pKa = 10.12ISSNIGNYY10 pKa = 8.42EE11 pKa = 4.54CYY13 pKa = 10.23TIDD16 pKa = 3.7EE17 pKa = 4.38MKK19 pKa = 10.76GYY21 pKa = 9.94ILKK24 pKa = 10.33CAEE27 pKa = 4.09NVFDD31 pKa = 5.01EE32 pKa = 4.58LWISKK37 pKa = 10.19DD38 pKa = 3.38KK39 pKa = 11.02NDD41 pKa = 3.7YY42 pKa = 9.63PILVILINDD51 pKa = 4.17NYY53 pKa = 11.14ACLHH57 pKa = 6.05FFIDD61 pKa = 4.16DD62 pKa = 3.74KK63 pKa = 11.34GTVFQSVGNGYY74 pKa = 10.6EE75 pKa = 4.4DD76 pKa = 4.73ISFKK80 pKa = 11.15LNNDD84 pKa = 4.36EE85 pKa = 3.9ITMPANSVILLDD97 pKa = 4.96DD98 pKa = 5.22AIKK101 pKa = 10.65CAEE104 pKa = 4.3QFFIDD109 pKa = 4.62CDD111 pKa = 3.63KK112 pKa = 10.82PNCIKK117 pKa = 10.39WSSLL121 pKa = 3.25

Molecular weight: 13.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J4UK85|J4UK85_9FIRM Allophanate hydrolase subunit 2 OS=Peptostreptococcaceae bacterium AS15 OX=936556 GN=HMPREF1142_0503 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.62QPKK9 pKa = 9.01KK10 pKa = 7.61RR11 pKa = 11.84QRR13 pKa = 11.84KK14 pKa = 8.25RR15 pKa = 11.84EE16 pKa = 3.66HH17 pKa = 6.26GFRR20 pKa = 11.84KK21 pKa = 9.99RR22 pKa = 11.84MSTPGGKK29 pKa = 8.83RR30 pKa = 11.84VLKK33 pKa = 10.27NRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.16GRR40 pKa = 11.84KK41 pKa = 9.06KK42 pKa = 9.67LTAA45 pKa = 4.2

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2299

0

2299

731109

32

2850

318.0

36.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.588 ± 0.049

1.008 ± 0.022

6.31 ± 0.04

7.168 ± 0.062

4.542 ± 0.043

5.883 ± 0.057

1.304 ± 0.019

9.529 ± 0.063

9.492 ± 0.055

8.532 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.026

5.969 ± 0.045

2.64 ± 0.032

2.774 ± 0.027

3.356 ± 0.034

6.753 ± 0.036

5.009 ± 0.045

6.482 ± 0.046

0.556 ± 0.013

4.263 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski