Methylobacterium sp. WL8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8WLN6|A0A5C8WLN6_9RHIZ TniQ family protein OS=Methylobacterium sp. WL8 OX=2603899 GN=FV234_23610 PE=4 SV=1
MM1 pKa = 7.8AIDD4 pKa = 4.63PNNLSATATLTFSEE18 pKa = 4.67EE19 pKa = 3.45FDD21 pKa = 5.37AFEE24 pKa = 4.21TWNGTTGLDD33 pKa = 4.14TIGAPQWSTKK43 pKa = 9.07IRR45 pKa = 11.84ATGTLPYY52 pKa = 10.09NDD54 pKa = 3.71EE55 pKa = 3.95SQYY58 pKa = 11.43YY59 pKa = 8.83VAGPDD64 pKa = 3.31GAAAAGNTLPNPFHH78 pKa = 6.42VADD81 pKa = 4.31GALTIAAAPADD92 pKa = 4.02PSIQGSLEE100 pKa = 3.78GQTYY104 pKa = 7.7TSGMITTYY112 pKa = 10.84HH113 pKa = 6.61EE114 pKa = 4.64FSQTYY119 pKa = 9.99GYY121 pKa = 11.16FEE123 pKa = 4.19MRR125 pKa = 11.84AEE127 pKa = 4.18LPAGHH132 pKa = 6.56GLWPAFWMLPEE143 pKa = 5.23DD144 pKa = 4.52GSWPPEE150 pKa = 3.66LDD152 pKa = 3.11

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8WY01|A0A5C8WY01_9RHIZ Aminotransferase class V-fold PLP-dependent enzyme OS=Methylobacterium sp. WL8 OX=2603899 GN=FV234_05300 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.77GRR39 pKa = 11.84KK40 pKa = 9.31KK41 pKa = 10.66LSAA44 pKa = 3.99

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4923

0

4923

1474374

27

5195

299.5

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.455 ± 0.054

0.812 ± 0.01

5.74 ± 0.035

5.254 ± 0.042

3.401 ± 0.023

9.171 ± 0.065

1.948 ± 0.019

4.381 ± 0.024

2.641 ± 0.03

10.234 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.019

2.138 ± 0.034

5.683 ± 0.035

2.688 ± 0.022

7.964 ± 0.052

4.9 ± 0.025

5.473 ± 0.049

7.783 ± 0.029

1.221 ± 0.014

2.012 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski