Streptococcus satellite phage Javan653

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZVR4|A0A4D5ZVR4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan653 OX=2558808 GN=JavanS653_0006 PE=4 SV=1
MM1 pKa = 7.38EE2 pKa = 4.98NKK4 pKa = 10.25EE5 pKa = 4.23PFDD8 pKa = 3.84LAKK11 pKa = 10.66SRR13 pKa = 11.84AEE15 pKa = 3.92NFGLDD20 pKa = 3.84LEE22 pKa = 4.5EE23 pKa = 6.71AYY25 pKa = 9.42DD26 pKa = 3.68TMLAFSLEE34 pKa = 4.17NKK36 pKa = 9.48FDD38 pKa = 3.95CYY40 pKa = 10.92SIEE43 pKa = 4.08EE44 pKa = 4.21RR45 pKa = 11.84NQLEE49 pKa = 4.34RR50 pKa = 11.84VLEE53 pKa = 4.07TLMDD57 pKa = 4.76FSDD60 pKa = 3.22MWMNGQIILVGKK72 pKa = 9.21EE73 pKa = 3.56RR74 pKa = 11.84EE75 pKa = 4.28AIEE78 pKa = 3.95

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZY35|A0A4D5ZY35_9VIRU PriCT_1 domain-containing protein OS=Streptococcus satellite phage Javan653 OX=2558808 GN=JavanS653_0011 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.26INEE5 pKa = 4.22IKK7 pKa = 10.67KK8 pKa = 9.76KK9 pKa = 10.54DD10 pKa = 3.31GSTVYY15 pKa = 9.17RR16 pKa = 11.84TNIYY20 pKa = 10.42LGVDD24 pKa = 4.01VITGKK29 pKa = 10.74KK30 pKa = 7.55VTTKK34 pKa = 9.32VTARR38 pKa = 11.84TKK40 pKa = 10.91KK41 pKa = 9.48EE42 pKa = 4.24LKK44 pKa = 9.65TKK46 pKa = 10.08AQQAHH51 pKa = 6.62FDD53 pKa = 4.12FKK55 pKa = 11.67ANGSTRR61 pKa = 11.84YY62 pKa = 9.46KK63 pKa = 10.03EE64 pKa = 4.34VVIEE68 pKa = 4.32TYY70 pKa = 10.86EE71 pKa = 4.01EE72 pKa = 4.44LASSWWDD79 pKa = 3.22NYY81 pKa = 10.79KK82 pKa = 9.06NTVKK86 pKa = 10.69VNTRR90 pKa = 11.84NTQKK94 pKa = 11.11SLLNNHH100 pKa = 5.74VLPLFGEE107 pKa = 4.71FKK109 pKa = 10.72LNKK112 pKa = 8.8LTTPVIQSMMNKK124 pKa = 9.77LANSTNTGEE133 pKa = 4.13VGAYY137 pKa = 8.68LHH139 pKa = 6.32YY140 pKa = 10.97GKK142 pKa = 9.58IHH144 pKa = 5.96TLNKK148 pKa = 10.19RR149 pKa = 11.84ILQYY153 pKa = 11.17GVILQVIPTNPANNVVLPRR172 pKa = 11.84NTQKK176 pKa = 11.1DD177 pKa = 3.22KK178 pKa = 10.56KK179 pKa = 10.89AKK181 pKa = 9.81VKK183 pKa = 10.6HH184 pKa = 5.76FNNDD188 pKa = 2.8EE189 pKa = 4.08LKK191 pKa = 10.82QFLTYY196 pKa = 10.53LDD198 pKa = 4.07SLDD201 pKa = 3.34NSKK204 pKa = 11.04YY205 pKa = 10.38KK206 pKa = 10.64NYY208 pKa = 10.65YY209 pKa = 10.47DD210 pKa = 3.03ITLYY214 pKa = 10.98KK215 pKa = 10.3FLLATGCRR223 pKa = 11.84INEE226 pKa = 4.0ALALSWSDD234 pKa = 2.86IDD236 pKa = 4.49LDD238 pKa = 3.79NSVVHH243 pKa = 5.55ITKK246 pKa = 9.12TLNCDD251 pKa = 3.19MKK253 pKa = 11.32LNSPKK258 pKa = 10.63SKK260 pKa = 10.55AGYY263 pKa = 9.68RR264 pKa = 11.84DD265 pKa = 3.11IDD267 pKa = 3.33IDD269 pKa = 3.84QQTVTMLKK277 pKa = 10.04RR278 pKa = 11.84YY279 pKa = 7.55QRR281 pKa = 11.84KK282 pKa = 6.4QTQEE286 pKa = 3.04AWKK289 pKa = 10.12LGRR292 pKa = 11.84TEE294 pKa = 4.07TVVFSDD300 pKa = 5.91FINAYY305 pKa = 9.07PNYY308 pKa = 9.28KK309 pKa = 10.09AVAGRR314 pKa = 11.84LKK316 pKa = 9.7THH318 pKa = 6.54FKK320 pKa = 10.33KK321 pKa = 10.78AGVNNIGFHH330 pKa = 5.79GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.58ASLLLNSGIPYY347 pKa = 10.0KK348 pKa = 10.34EE349 pKa = 3.8LQHH352 pKa = 6.73RR353 pKa = 11.84LGHH356 pKa = 5.68STLSMTMDD364 pKa = 4.17TYY366 pKa = 11.8SHH368 pKa = 7.15LSKK371 pKa = 11.01EE372 pKa = 4.3NAKK375 pKa = 10.31KK376 pKa = 9.35ATSFYY381 pKa = 10.1EE382 pKa = 3.9QALKK386 pKa = 10.8SII388 pKa = 4.54

Molecular weight: 44.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

3157

61

507

225.5

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.258 ± 0.539

0.792 ± 0.207

6.018 ± 0.433

6.684 ± 0.674

4.593 ± 0.378

4.973 ± 0.408

1.996 ± 0.292

7.19 ± 0.57

9.344 ± 0.51

10.105 ± 0.426

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.266

6.335 ± 0.604

3.294 ± 0.395

3.484 ± 0.253

4.91 ± 0.501

5.828 ± 0.386

6.177 ± 0.518

5.353 ± 0.49

0.824 ± 0.139

4.878 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski