Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Paraneoptera; Thysanoptera;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20529 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J1T7W2|A0A6J1T7W2_FRAOC myotubularin-related protein 10-A isoform X1 OS=Frankliniella occidentalis OX=133901 GN=LOC113214529 PE=3 SV=1
MM1 pKa = 7.67PSLMEE6 pKa = 4.24LVGEE10 pKa = 4.51PGAAPGVVVDD20 pKa = 6.24DD21 pKa = 4.48EE22 pKa = 4.53VLDD25 pKa = 3.96YY26 pKa = 11.32DD27 pKa = 4.34ALLNVSANGSLPRR40 pKa = 11.84NASLQEE46 pKa = 3.89DD47 pKa = 4.82VVFDD51 pKa = 3.86RR52 pKa = 11.84TDD54 pKa = 2.87VRR56 pKa = 11.84ALFIVLYY63 pKa = 9.31TLVFCCCFFGEE74 pKa = 4.84SLDD77 pKa = 4.33CGPPGPRR84 pKa = 11.84PGPGPAVHH92 pKa = 6.65FQDD95 pKa = 4.21QGAPP99 pKa = 3.41

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J1T5S9|A0A6J1T5S9_FRAOC general odorant-binding protein 71 OS=Frankliniella occidentalis OX=133901 GN=LOC113212498 PE=4 SV=1
MM1 pKa = 7.74AKK3 pKa = 9.94QNAAAEE9 pKa = 4.57GNRR12 pKa = 11.84TLARR16 pKa = 11.84ASPGPSGPGPRR27 pKa = 11.84RR28 pKa = 11.84GPRR31 pKa = 11.84RR32 pKa = 11.84AASPGRR38 pKa = 11.84GRR40 pKa = 11.84GPGRR44 pKa = 11.84GEE46 pKa = 3.83SLGRR50 pKa = 11.84GASPGRR56 pKa = 11.84GPGPGRR62 pKa = 11.84GPGRR66 pKa = 11.84GASPGRR72 pKa = 11.84GPAPASHH79 pKa = 6.73TARR82 pKa = 11.84HH83 pKa = 5.73RR84 pKa = 11.84PVVAPRR90 pKa = 11.84GSGGLISASRR100 pKa = 11.84HH101 pKa = 3.93

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15639

4890

20529

12625742

34

19827

615.0

68.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.768 ± 0.023

1.966 ± 0.018

5.486 ± 0.014

6.425 ± 0.022

3.206 ± 0.012

6.543 ± 0.026

2.726 ± 0.013

3.981 ± 0.014

5.398 ± 0.022

9.129 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.008

3.894 ± 0.012

6.387 ± 0.028

4.594 ± 0.022

6.022 ± 0.02

8.769 ± 0.027

5.53 ± 0.018

6.456 ± 0.016

1.066 ± 0.006

2.457 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski