Limnoraphis robusta CS-951

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Limnoraphis; Limnoraphis robusta

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5Y8M8|A0A0F5Y8M8_9CYAN Germane domain-containing protein OS=Limnoraphis robusta CS-951 OX=1637645 GN=WN50_26070 PE=4 SV=1
MM1 pKa = 7.51SIDD4 pKa = 3.43NTVHH8 pKa = 5.96VKK10 pKa = 10.94AEE12 pKa = 4.11TRR14 pKa = 11.84NHH16 pKa = 6.29KK17 pKa = 10.69GFFVQQAAYY26 pKa = 8.6EE27 pKa = 4.22LKK29 pKa = 10.91SIDD32 pKa = 3.57DD33 pKa = 4.18SGWALICLDD42 pKa = 4.79DD43 pKa = 4.22AVCYY47 pKa = 10.59YY48 pKa = 10.94VDD50 pKa = 4.44PDD52 pKa = 3.85NLEE55 pKa = 4.14SDD57 pKa = 3.82SDD59 pKa = 4.01DD60 pKa = 4.05DD61 pKa = 4.84SEE63 pKa = 4.44EE64 pKa = 4.44SEE66 pKa = 4.44SS67 pKa = 3.65

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J9EWB6|A0A0J9EWB6_9CYAN Aldose epimerase (Fragment) OS=Limnoraphis robusta CS-951 OX=1637645 GN=WN50_35880 PE=4 SV=1
MM1 pKa = 7.42AQQTLKK7 pKa = 9.73GTNRR11 pKa = 11.84KK12 pKa = 8.73KK13 pKa = 10.69KK14 pKa = 8.48RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.42NGQAVIKK33 pKa = 10.36ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.19KK38 pKa = 9.97GRR40 pKa = 11.84ARR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5035

0

5035

1616785

29

3368

321.1

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.038

1.03 ± 0.014

4.866 ± 0.033

6.748 ± 0.036

4.092 ± 0.025

6.448 ± 0.044

1.829 ± 0.02

6.907 ± 0.034

4.888 ± 0.037

11.012 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.017

4.48 ± 0.032

4.722 ± 0.028

5.673 ± 0.034

4.996 ± 0.033

6.618 ± 0.029

5.598 ± 0.029

6.406 ± 0.028

1.445 ± 0.017

3.099 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski