Amino acid dipepetide frequency for Micromonospora sp. MW-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	24.079AlaAla: 24.079 ± 0.193	1.022AlaCys: 1.022 ± 0.028	9.355AlaAsp: 9.355 ± 0.087	8.55AlaGlu: 8.55 ± 0.08	3.235AlaPhe: 3.235 ± 0.042	16.085AlaGly: 16.085 ± 0.135	2.6AlaHis: 2.6 ± 0.039	3.517AlaIle: 3.517 ± 0.052	2.179AlaLys: 2.179 ± 0.043	14.882AlaLeu: 14.882 ± 0.129	2.332AlaMet: 2.332 ± 0.038	2.038AlaAsn: 2.038 ± 0.036	7.68AlaPro: 7.68 ± 0.086	3.549AlaGln: 3.549 ± 0.043	11.222AlaArg: 11.222 ± 0.09	5.469AlaSer: 5.469 ± 0.063	8.047AlaThr: 8.047 ± 0.076	13.34AlaVal: 13.34 ± 0.127	2.002AlaTrp: 2.002 ± 0.034	2.801AlaTyr: 2.801 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.049CysAla: 1.049 ± 0.024	0.102CysCys: 0.102 ± 0.008	0.494CysAsp: 0.494 ± 0.019	0.33CysGlu: 0.33 ± 0.012	0.204CysPhe: 0.204 ± 0.01	0.966CysGly: 0.966 ± 0.023	0.205CysHis: 0.205 ± 0.012	0.129CysIle: 0.129 ± 0.008	0.091CysLys: 0.091 ± 0.007	0.717CysLeu: 0.717 ± 0.018	0.102CysMet: 0.102 ± 0.008	0.126CysAsn: 0.126 ± 0.007	0.538CysPro: 0.538 ± 0.018	0.198CysGln: 0.198 ± 0.011	0.671CysArg: 0.671 ± 0.019	0.392CysSer: 0.392 ± 0.016	0.429CysThr: 0.429 ± 0.016	0.657CysVal: 0.657 ± 0.019	0.144CysTrp: 0.144 ± 0.008	0.168CysTyr: 0.168 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.906AspAla: 7.906 ± 0.067	0.427AspCys: 0.427 ± 0.016	3.719AspAsp: 3.719 ± 0.049	3.721AspGlu: 3.721 ± 0.048	1.423AspPhe: 1.423 ± 0.03	6.473AspGly: 6.473 ± 0.067	1.272AspHis: 1.272 ± 0.025	1.506AspIle: 1.506 ± 0.028	0.838AspLys: 0.838 ± 0.024	6.664AspLeu: 6.664 ± 0.065	0.672AspMet: 0.672 ± 0.016	0.897AspAsn: 0.897 ± 0.022	5.109AspPro: 5.109 ± 0.059	1.708AspGln: 1.708 ± 0.032	5.95AspArg: 5.95 ± 0.062	2.201AspSer: 2.201 ± 0.036	2.787AspThr: 2.787 ± 0.035	5.092AspVal: 5.092 ± 0.058	1.037AspTrp: 1.037 ± 0.025	1.036AspTyr: 1.036 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.417GluAla: 6.417 ± 0.065	0.33GluCys: 0.33 ± 0.014	1.983GluAsp: 1.983 ± 0.035	2.391GluGlu: 2.391 ± 0.044	1.419GluPhe: 1.419 ± 0.027	3.139GluGly: 3.139 ± 0.048	1.386GluHis: 1.386 ± 0.028	2.014GluIle: 2.014 ± 0.033	0.913GluLys: 0.913 ± 0.028	6.674GluLeu: 6.674 ± 0.066	0.76GluMet: 0.76 ± 0.02	0.765GluAsn: 0.765 ± 0.018	3.478GluPro: 3.478 ± 0.059	2.096GluGln: 2.096 ± 0.039	5.128GluArg: 5.128 ± 0.063	2.109GluSer: 2.109 ± 0.039	2.314GluThr: 2.314 ± 0.035	4.619GluVal: 4.619 ± 0.048	0.661GluTrp: 0.661 ± 0.018	1.043GluTyr: 1.043 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.701PheAla: 3.701 ± 0.042	0.24PheCys: 0.24 ± 0.011	1.943PheAsp: 1.943 ± 0.033	1.102PheGlu: 1.102 ± 0.027	0.79PhePhe: 0.79 ± 0.023	3.005PheGly: 3.005 ± 0.045	0.559PheHis: 0.559 ± 0.015	0.578PheIle: 0.578 ± 0.018	0.363PheLys: 0.363 ± 0.016	2.423PheLeu: 2.423 ± 0.037	0.302PheMet: 0.302 ± 0.012	0.524PheAsn: 0.524 ± 0.018	1.347PhePro: 1.347 ± 0.026	0.599PheGln: 0.599 ± 0.02	1.775PheArg: 1.775 ± 0.029	1.276PheSer: 1.276 ± 0.027	1.831PheThr: 1.831 ± 0.029	2.423PheVal: 2.423 ± 0.034	0.431PheTrp: 0.431 ± 0.015	0.54PheTyr: 0.54 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.735GlyAla: 11.735 ± 0.102	0.871GlyCys: 0.871 ± 0.022	5.492GlyAsp: 5.492 ± 0.069	4.882GlyGlu: 4.882 ± 0.063	2.709GlyPhe: 2.709 ± 0.038	9.626GlyGly: 9.626 ± 0.113	2.254GlyHis: 2.254 ± 0.044	2.981GlyIle: 2.981 ± 0.043	1.756GlyLys: 1.756 ± 0.034	9.919GlyLeu: 9.919 ± 0.092	1.914GlyMet: 1.914 ± 0.033	1.734GlyAsn: 1.734 ± 0.036	6.092GlyPro: 6.092 ± 0.069	2.953GlyGln: 2.953 ± 0.054	8.862GlyArg: 8.862 ± 0.067	4.748GlySer: 4.748 ± 0.057	5.835GlyThr: 5.835 ± 0.066	8.314GlyVal: 8.314 ± 0.093	2.062GlyTrp: 2.062 ± 0.039	2.397GlyTyr: 2.397 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.513HisAla: 2.513 ± 0.04	0.188HisCys: 0.188 ± 0.01	1.336HisAsp: 1.336 ± 0.026	1.038HisGlu: 1.038 ± 0.024	0.545HisPhe: 0.545 ± 0.017	2.166HisGly: 2.166 ± 0.04	0.646HisHis: 0.646 ± 0.02	0.484HisIle: 0.484 ± 0.018	0.234HisLys: 0.234 ± 0.011	2.472HisLeu: 2.472 ± 0.037	0.24HisMet: 0.24 ± 0.011	0.348HisAsn: 0.348 ± 0.014	1.846HisPro: 1.846 ± 0.036	0.613HisGln: 0.613 ± 0.019	2.271HisArg: 2.271 ± 0.049	0.869HisSer: 0.869 ± 0.02	1.062HisThr: 1.062 ± 0.024	1.716HisVal: 1.716 ± 0.036	0.339HisTrp: 0.339 ± 0.014	0.398HisTyr: 0.398 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.383IleAla: 4.383 ± 0.049	0.281IleCys: 0.281 ± 0.011	2.093IleAsp: 2.093 ± 0.033	1.76IleGlu: 1.76 ± 0.033	0.761IlePhe: 0.761 ± 0.021	3.286IleGly: 3.286 ± 0.039	0.511IleHis: 0.511 ± 0.016	0.831IleIle: 0.831 ± 0.024	0.555IleLys: 0.555 ± 0.017	2.191IleLeu: 2.191 ± 0.042	0.419IleMet: 0.419 ± 0.015	0.67IleAsn: 0.67 ± 0.019	1.472IlePro: 1.472 ± 0.03	0.619IleGln: 0.619 ± 0.021	2.27IleArg: 2.27 ± 0.032	1.499IleSer: 1.499 ± 0.027	1.878IleThr: 1.878 ± 0.037	2.643IleVal: 2.643 ± 0.039	0.351IleTrp: 0.351 ± 0.014	0.518IleTyr: 0.518 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	1.957LysAla: 1.957 ± 0.041	0.102LysCys: 0.102 ± 0.008	0.734LysAsp: 0.734 ± 0.024	0.688LysGlu: 0.688 ± 0.02	0.37LysPhe: 0.37 ± 0.015	1.154LysGly: 1.154 ± 0.027	0.305LysHis: 0.305 ± 0.013	0.717LysIle: 0.717 ± 0.021	0.463LysLys: 0.463 ± 0.02	1.675LysLeu: 1.675 ± 0.034	0.282LysMet: 0.282 ± 0.012	0.337LysAsn: 0.337 ± 0.015	1.037LysPro: 1.037 ± 0.026	0.527LysGln: 0.527 ± 0.017	1.166LysArg: 1.166 ± 0.027	0.836LysSer: 0.836 ± 0.024	0.94LysThr: 0.94 ± 0.024	1.442LysVal: 1.442 ± 0.031	0.195LysTrp: 0.195 ± 0.01	0.312LysTyr: 0.312 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	17.487LeuAla: 17.487 ± 0.144	0.758LeuCys: 0.758 ± 0.019	7.043LeuAsp: 7.043 ± 0.073	3.675LeuGlu: 3.675 ± 0.052	2.503LeuPhe: 2.503 ± 0.044	9.536LeuGly: 9.536 ± 0.087	2.284LeuHis: 2.284 ± 0.036	3.001LeuIle: 3.001 ± 0.05	1.258LeuLys: 1.258 ± 0.029	11.87LeuLeu: 11.87 ± 0.129	1.259LeuMet: 1.259 ± 0.024	1.537LeuAsn: 1.537 ± 0.031	6.9LeuPro: 6.9 ± 0.067	1.821LeuGln: 1.821 ± 0.032	9.571LeuArg: 9.571 ± 0.083	4.728LeuSer: 4.728 ± 0.059	6.953LeuThr: 6.953 ± 0.064	10.261LeuVal: 10.261 ± 0.098	1.242LeuTrp: 1.242 ± 0.029	1.666LeuTyr: 1.666 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	1.945MetAla: 1.945 ± 0.032	0.097MetCys: 0.097 ± 0.007	0.682MetAsp: 0.682 ± 0.017	0.543MetGlu: 0.543 ± 0.019	0.434MetPhe: 0.434 ± 0.015	1.036MetGly: 1.036 ± 0.023	0.276MetHis: 0.276 ± 0.014	0.648MetIle: 0.648 ± 0.019	0.287MetLys: 0.287 ± 0.013	1.603MetLeu: 1.603 ± 0.031	0.237MetMet: 0.237 ± 0.011	0.328MetAsn: 0.328 ± 0.013	1.051MetPro: 1.051 ± 0.025	0.41MetGln: 0.41 ± 0.015	1.344MetArg: 1.344 ± 0.027	1.143MetSer: 1.143 ± 0.025	1.543MetThr: 1.543 ± 0.027	1.266MetVal: 1.266 ± 0.025	0.181MetTrp: 0.181 ± 0.009	0.273MetTyr: 0.273 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.081AsnAla: 2.081 ± 0.032	0.169AsnCys: 0.169 ± 0.011	0.875AsnAsp: 0.875 ± 0.021	0.711AsnGlu: 0.711 ± 0.022	0.478AsnPhe: 0.478 ± 0.014	1.696AsnGly: 1.696 ± 0.039	0.362AsnHis: 0.362 ± 0.013	0.52AsnIle: 0.52 ± 0.016	0.318AsnLys: 0.318 ± 0.013	1.763AsnLeu: 1.763 ± 0.033	0.261AsnMet: 0.261 ± 0.013	0.431AsnAsn: 0.431 ± 0.018	1.39AsnPro: 1.39 ± 0.033	0.527AsnGln: 0.527 ± 0.018	1.352AsnArg: 1.352 ± 0.027	0.827AsnSer: 0.827 ± 0.023	0.949AsnThr: 0.949 ± 0.027	1.311AsnVal: 1.311 ± 0.025	0.306AsnTrp: 0.306 ± 0.014	0.398AsnTyr: 0.398 ± 0.016	0.0AsnXaa: 0.0 ± 0.0
Pro	11.124ProAla: 11.124 ± 0.13	0.33ProCys: 0.33 ± 0.014	4.789ProAsp: 4.789 ± 0.059	3.798ProGlu: 3.798 ± 0.049	1.399ProPhe: 1.399 ± 0.029	7.423ProGly: 7.423 ± 0.09	1.222ProHis: 1.222 ± 0.025	1.464ProIle: 1.464 ± 0.028	0.929ProLys: 0.929 ± 0.022	5.444ProLeu: 5.444 ± 0.055	0.987ProMet: 0.987 ± 0.025	0.968ProAsn: 0.968 ± 0.024	4.619ProPro: 4.619 ± 0.094	1.634ProGln: 1.634 ± 0.03	4.339ProArg: 4.339 ± 0.057	2.948ProSer: 2.948 ± 0.052	3.976ProThr: 3.976 ± 0.058	6.372ProVal: 6.372 ± 0.072	0.972ProTrp: 0.972 ± 0.028	1.267ProTyr: 1.267 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.646GlnAla: 3.646 ± 0.047	0.146GlnCys: 0.146 ± 0.009	1.071GlnAsp: 1.071 ± 0.024	1.097GlnGlu: 1.097 ± 0.027	0.72GlnPhe: 0.72 ± 0.019	1.914GlnGly: 1.914 ± 0.036	0.626GlnHis: 0.626 ± 0.023	1.011GlnIle: 1.011 ± 0.03	0.394GlnLys: 0.394 ± 0.016	3.142GlnLeu: 3.142 ± 0.043	0.392GlnMet: 0.392 ± 0.015	0.432GlnAsn: 0.432 ± 0.017	1.964GlnPro: 1.964 ± 0.036	1.202GlnGln: 1.202 ± 0.032	2.787GlnArg: 2.787 ± 0.054	1.085GlnSer: 1.085 ± 0.023	1.224GlnThr: 1.224 ± 0.027	2.812GlnVal: 2.812 ± 0.045	0.477GlnTrp: 0.477 ± 0.013	0.495GlnTyr: 0.495 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	11.152ArgAla: 11.152 ± 0.088	0.735ArgCys: 0.735 ± 0.021	4.76ArgAsp: 4.76 ± 0.054	4.354ArgGlu: 4.354 ± 0.051	2.445ArgPhe: 2.445 ± 0.038	6.267ArgGly: 6.267 ± 0.066	2.293ArgHis: 2.293 ± 0.041	3.058ArgIle: 3.058 ± 0.046	1.206ArgLys: 1.206 ± 0.024	9.878ArgLeu: 9.878 ± 0.1	1.833ArgMet: 1.833 ± 0.029	1.384ArgAsn: 1.384 ± 0.026	6.198ArgPro: 6.198 ± 0.076	2.658ArgGln: 2.658 ± 0.04	9.517ArgArg: 9.517 ± 0.109	4.218ArgSer: 4.218 ± 0.055	4.815ArgThr: 4.815 ± 0.052	6.915ArgVal: 6.915 ± 0.064	1.74ArgTrp: 1.74 ± 0.031	2.06ArgTyr: 2.06 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.226SerAla: 6.226 ± 0.066	0.397SerCys: 0.397 ± 0.016	2.277SerAsp: 2.277 ± 0.036	1.764SerGlu: 1.764 ± 0.033	1.39SerPhe: 1.39 ± 0.033	5.333SerGly: 5.333 ± 0.066	0.827SerHis: 0.827 ± 0.02	1.391SerIle: 1.391 ± 0.031	0.712SerLys: 0.712 ± 0.021	4.185SerLeu: 4.185 ± 0.052	0.923SerMet: 0.923 ± 0.021	0.822SerAsn: 0.822 ± 0.026	3.135SerPro: 3.135 ± 0.043	1.079SerGln: 1.079 ± 0.022	3.735SerArg: 3.735 ± 0.048	2.517SerSer: 2.517 ± 0.054	3.107SerThr: 3.107 ± 0.048	3.983SerVal: 3.983 ± 0.049	0.874SerTrp: 0.874 ± 0.018	1.164SerTyr: 1.164 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	8.625ThrAla: 8.625 ± 0.082	0.448ThrCys: 0.448 ± 0.016	3.596ThrAsp: 3.596 ± 0.044	2.82ThrGlu: 2.82 ± 0.039	1.485ThrPhe: 1.485 ± 0.026	6.987ThrGly: 6.987 ± 0.075	1.03ThrHis: 1.03 ± 0.024	1.718ThrIle: 1.718 ± 0.035	0.896ThrLys: 0.896 ± 0.023	5.568ThrLeu: 5.568 ± 0.059	0.907ThrMet: 0.907 ± 0.022	1.038ThrAsn: 1.038 ± 0.028	4.137ThrPro: 4.137 ± 0.046	1.136ThrGln: 1.136 ± 0.025	4.206ThrArg: 4.206 ± 0.045	2.838ThrSer: 2.838 ± 0.046	3.577ThrThr: 3.577 ± 0.065	6.211ThrVal: 6.211 ± 0.07	0.891ThrTrp: 0.891 ± 0.024	1.209ThrTyr: 1.209 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	13.311ValAla: 13.311 ± 0.113	0.726ValCys: 0.726 ± 0.019	6.082ValAsp: 6.082 ± 0.067	4.836ValGlu: 4.836 ± 0.057	2.338ValPhe: 2.338 ± 0.036	7.954ValGly: 7.954 ± 0.085	1.834ValHis: 1.834 ± 0.033	2.567ValIle: 2.567 ± 0.036	1.304ValLys: 1.304 ± 0.032	10.06ValLeu: 10.06 ± 0.093	1.073ValMet: 1.073 ± 0.023	1.624ValAsn: 1.624 ± 0.029	5.827ValPro: 5.827 ± 0.069	2.026ValGln: 2.026 ± 0.038	7.735ValArg: 7.735 ± 0.075	4.267ValSer: 4.267 ± 0.047	6.034ValThr: 6.034 ± 0.074	9.089ValVal: 9.089 ± 0.09	1.114ValTrp: 1.114 ± 0.023	1.481ValTyr: 1.481 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.802TrpAla: 1.802 ± 0.031	0.168TrpCys: 0.168 ± 0.009	0.735TrpAsp: 0.735 ± 0.023	0.605TrpGlu: 0.605 ± 0.015	0.517TrpPhe: 0.517 ± 0.016	1.005TrpGly: 1.005 ± 0.026	0.407TrpHis: 0.407 ± 0.015	0.49TrpIle: 0.49 ± 0.016	0.268TrpLys: 0.268 ± 0.011	1.979TrpLeu: 1.979 ± 0.037	0.257TrpMet: 0.257 ± 0.011	0.364TrpAsn: 0.364 ± 0.014	0.965TrpPro: 0.965 ± 0.023	0.613TrpGln: 0.613 ± 0.018	1.678TrpArg: 1.678 ± 0.03	1.008TrpSer: 1.008 ± 0.022	1.007TrpThr: 1.007 ± 0.025	1.116TrpVal: 1.116 ± 0.023	0.41TrpTrp: 0.41 ± 0.016	0.386TrpTyr: 0.386 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.847TyrAla: 2.847 ± 0.039	0.174TyrCys: 0.174 ± 0.009	1.381TyrAsp: 1.381 ± 0.039	0.972TyrGlu: 0.972 ± 0.023	0.588TyrPhe: 0.588 ± 0.018	2.089TyrGly: 2.089 ± 0.037	0.426TyrHis: 0.426 ± 0.015	0.364TyrIle: 0.364 ± 0.013	0.273TyrLys: 0.273 ± 0.011	2.247TyrLeu: 2.247 ± 0.036	0.172TyrMet: 0.172 ± 0.009	0.357TyrAsn: 0.357 ± 0.017	1.217TyrPro: 1.217 ± 0.025	0.669TyrGln: 0.669 ± 0.017	1.909TyrArg: 1.909 ± 0.036	0.861TyrSer: 0.861 ± 0.023	1.017TyrThr: 1.017 ± 0.029	1.697TyrVal: 1.697 ± 0.034	0.35TyrTrp: 0.35 ± 0.014	0.422TyrTyr: 0.422 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6184 proteins (2048476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---