Synechococcus sp. SynAce01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0P7T2|A0A1J0P7T2_9SYNE Plasma-membrane proton-efflux P-type ATPase OS=Synechococcus sp. SynAce01 OX=1916956 GN=BM449_01575 PE=3 SV=1
MM1 pKa = 7.71PAPVPWSLAWSEE13 pKa = 4.2SGEE16 pKa = 4.01LALADD21 pKa = 4.8RR22 pKa = 11.84LDD24 pKa = 4.16LLHH27 pKa = 6.37TLIMSEE33 pKa = 4.51SPDD36 pKa = 3.39IQPTLIASLEE46 pKa = 4.41AISLEE51 pKa = 4.44EE52 pKa = 3.99VSSLCTDD59 pKa = 3.96GLTGTCAA66 pKa = 3.33

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0P8C8|A0A1J0P8C8_9SYNE Peptidase M23 OS=Synechococcus sp. SynAce01 OX=1916956 GN=BM449_02945 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.92GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2626

0

2626

760367

30

2199

289.6

31.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.751 ± 0.064

1.166 ± 0.018

4.792 ± 0.036

5.73 ± 0.052

3.169 ± 0.035

8.741 ± 0.052

2.006 ± 0.027

4.055 ± 0.038

2.269 ± 0.039

13.229 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.019

2.287 ± 0.025

6.024 ± 0.042

4.601 ± 0.036

7.436 ± 0.049

6.161 ± 0.036

4.421 ± 0.03

6.837 ± 0.041

1.741 ± 0.026

1.778 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski