McMurdo Ice Shelf pond-associated circular DNA virus-7

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075M3Q4|A0A075M3Q4_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-7 OX=1521391 PE=4 SV=1
MM1 pKa = 7.26PQPRR5 pKa = 11.84MKK7 pKa = 10.82DD8 pKa = 3.34NEE10 pKa = 4.21VKK12 pKa = 10.55KK13 pKa = 10.98VFNALVFTDD22 pKa = 4.28FSLYY26 pKa = 10.49KK27 pKa = 8.75WTLEE31 pKa = 3.96DD32 pKa = 4.04LATHH36 pKa = 7.3KK37 pKa = 10.29IQYY40 pKa = 8.85LAYY43 pKa = 9.81GLEE46 pKa = 4.35TCPEE50 pKa = 4.25TKK52 pKa = 9.94KK53 pKa = 9.98QHH55 pKa = 5.39HH56 pKa = 6.07QGWLYY61 pKa = 11.26ASTDD65 pKa = 3.31RR66 pKa = 11.84KK67 pKa = 10.05RR68 pKa = 11.84SFMAWKK74 pKa = 10.03KK75 pKa = 9.95HH76 pKa = 4.1FQLLGLDD83 pKa = 3.39MHH85 pKa = 6.87YY86 pKa = 11.03EE87 pKa = 3.96AMQGNFSQNTKK98 pKa = 10.24YY99 pKa = 10.21ISKK102 pKa = 10.1EE103 pKa = 3.93GQLTEE108 pKa = 5.34LGVKK112 pKa = 10.17PMGSGKK118 pKa = 9.99KK119 pKa = 8.32RR120 pKa = 11.84TLLEE124 pKa = 3.8YY125 pKa = 10.37KK126 pKa = 10.33KK127 pKa = 10.38QIEE130 pKa = 4.39AGAHH134 pKa = 4.94VLDD137 pKa = 4.65IAEE140 pKa = 4.38EE141 pKa = 4.21DD142 pKa = 3.7EE143 pKa = 4.54KK144 pKa = 11.35FGTFLQYY151 pKa = 10.72RR152 pKa = 11.84SGLNAYY158 pKa = 9.89ADD160 pKa = 3.53HH161 pKa = 6.52QRR163 pKa = 11.84FKK165 pKa = 10.99RR166 pKa = 11.84IRR168 pKa = 11.84TSRR171 pKa = 11.84EE172 pKa = 3.35KK173 pKa = 9.83PEE175 pKa = 3.64VYY177 pKa = 10.0IRR179 pKa = 11.84VGPPGTGKK187 pKa = 10.36SRR189 pKa = 11.84WLDD192 pKa = 3.22EE193 pKa = 4.32TYY195 pKa = 11.2GFDD198 pKa = 3.59KK199 pKa = 10.57WIEE202 pKa = 4.06APDD205 pKa = 3.78NSGKK209 pKa = 9.76WFDD212 pKa = 3.98GCDD215 pKa = 3.25LADD218 pKa = 3.86IIVFNDD224 pKa = 3.6VGPGSCPPLDD234 pKa = 3.44IFKK237 pKa = 10.23KK238 pKa = 10.6LCDD241 pKa = 3.62RR242 pKa = 11.84YY243 pKa = 9.89PFRR246 pKa = 11.84GAVKK250 pKa = 10.51GGFIWLKK257 pKa = 9.67PKK259 pKa = 10.48IIVFTSNSYY268 pKa = 5.38WTEE271 pKa = 3.29WFGPDD276 pKa = 4.36LAPLDD281 pKa = 3.73RR282 pKa = 11.84EE283 pKa = 4.34AIEE286 pKa = 4.27RR287 pKa = 11.84RR288 pKa = 11.84ITSTICVEE296 pKa = 4.09

Molecular weight: 34.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075M3Q4|A0A075M3Q4_9VIRU Putative capsid protein OS=McMurdo Ice Shelf pond-associated circular DNA virus-7 OX=1521391 PE=4 SV=1
MM1 pKa = 6.64VFKK4 pKa = 10.77RR5 pKa = 11.84KK6 pKa = 9.44FSSKK10 pKa = 9.9RR11 pKa = 11.84MGRR14 pKa = 11.84GSIRR18 pKa = 11.84KK19 pKa = 8.59PFIKK23 pKa = 10.25RR24 pKa = 11.84GTRR27 pKa = 11.84RR28 pKa = 11.84VGSAIRR34 pKa = 11.84NLAKK38 pKa = 10.08KK39 pKa = 9.61VRR41 pKa = 11.84RR42 pKa = 11.84ITSTIEE48 pKa = 3.8TKK50 pKa = 10.74SGVEE54 pKa = 4.1QITDD58 pKa = 3.87NINLRR63 pKa = 11.84HH64 pKa = 5.82NNQVTVSSAVLEE76 pKa = 4.6TQTGTLDD83 pKa = 3.57NEE85 pKa = 4.49NSRR88 pKa = 11.84GMRR91 pKa = 11.84IGDD94 pKa = 4.56KK95 pKa = 9.4ITLSNVQFTMMFEE108 pKa = 4.28LNEE111 pKa = 4.42AYY113 pKa = 9.69TDD115 pKa = 3.36VTLRR119 pKa = 11.84LMVVRR124 pKa = 11.84AAKK127 pKa = 10.4NDD129 pKa = 3.29IPNITDD135 pKa = 3.41MFHH138 pKa = 6.4GASGNKK144 pKa = 9.01MLDD147 pKa = 3.11TFNNEE152 pKa = 4.07RR153 pKa = 11.84YY154 pKa = 10.48SMLYY158 pKa = 8.88QKK160 pKa = 9.91YY161 pKa = 9.09VRR163 pKa = 11.84LRR165 pKa = 11.84SNPTAAVPSGTQFPGSGTYY184 pKa = 9.61QGPNQIMSRR193 pKa = 11.84ATKK196 pKa = 9.12IVKK199 pKa = 10.22FSVPGRR205 pKa = 11.84KK206 pKa = 8.91FSRR209 pKa = 11.84SGIIQYY215 pKa = 10.06QNNDD219 pKa = 3.27VQPKK223 pKa = 9.26FFDD226 pKa = 3.82YY227 pKa = 11.18YY228 pKa = 10.48FIVYY232 pKa = 9.86AYY234 pKa = 11.17SNFATVDD241 pKa = 3.53SGPVVYY247 pKa = 10.5NVAHH251 pKa = 6.75INDD254 pKa = 4.82CIIRR258 pKa = 11.84MHH260 pKa = 6.43YY261 pKa = 10.39KK262 pKa = 10.47DD263 pKa = 3.44AA264 pKa = 5.6

Molecular weight: 30.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

560

264

296

280.0

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.357 ± 0.035

1.071 ± 0.445

5.536 ± 0.636

4.821 ± 1.395

5.536 ± 0.15

7.143 ± 0.452

1.964 ± 0.289

6.25 ± 0.609

8.036 ± 1.026

6.25 ± 1.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.036 ± 0.727

5.179 ± 1.784

4.286 ± 0.563

3.929 ± 0.153

6.786 ± 0.995

6.25 ± 1.096

6.607 ± 0.623

5.893 ± 1.325

1.607 ± 1.033

4.464 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski