Sclerotinia sclerotiorum deltaflexivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Deltaflexiviridae; Deltaflexivirus; Sclerotinia deltaflexivirus 1

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125R921|A0A125R921_9VIRU Uncharacterized protein OS=Sclerotinia sclerotiorum deltaflexivirus 1 OX=1788309 PE=4 SV=1
MM1 pKa = 7.42TGSKK5 pKa = 10.15VLIMSALGATDD16 pKa = 3.69TPVSNLTSGVSQIGITSPPTPTDD39 pKa = 2.98IQIPFVQLFNPHH51 pKa = 4.02TKK53 pKa = 10.1YY54 pKa = 10.92RR55 pKa = 11.84LTAADD60 pKa = 3.47ATLRR64 pKa = 11.84AYY66 pKa = 10.58LATYY70 pKa = 10.04EE71 pKa = 4.26SVQFVSLSFAVEE83 pKa = 3.64ITGQNGKK90 pKa = 8.88LQFAATPSDD99 pKa = 3.31ISPSGDD105 pKa = 4.12LDD107 pKa = 3.49WLGAPVYY114 pKa = 10.73QRR116 pKa = 11.84FAGNAQGDD124 pKa = 3.87TFAEE128 pKa = 4.49YY129 pKa = 10.78NFPASHH135 pKa = 7.2PFGHH139 pKa = 6.18EE140 pKa = 3.82LKK142 pKa = 10.83AVALGNAPPRR152 pKa = 11.84FFFQFDD158 pKa = 3.55GSSGDD163 pKa = 3.36VARR166 pKa = 11.84IRR168 pKa = 11.84GRR170 pKa = 11.84LTLRR174 pKa = 11.84GGGRR178 pKa = 11.84GIIPAVSLTEE188 pKa = 3.92LATGKK193 pKa = 9.78NAATIKK199 pKa = 11.0CEE201 pKa = 4.16GEE203 pKa = 3.92NN204 pKa = 3.65

Molecular weight: 21.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125R922|A0A125R922_9VIRU Uncharacterized protein OS=Sclerotinia sclerotiorum deltaflexivirus 1 OX=1788309 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.69LFGRR6 pKa = 11.84FNLALMPLALVPLRR20 pKa = 11.84FYY22 pKa = 11.37SRR24 pKa = 11.84TNVTQKK30 pKa = 10.97ILMTTVGPIAMAFAMILMSIVFWGLLFCCFLPNLQFGFILGICHH74 pKa = 6.33YY75 pKa = 9.02MHH77 pKa = 7.01IPCLRR82 pKa = 11.84AIGIWGTLWSFLALQAVVSSSSSAVIFIGQVNLHH116 pKa = 6.89PMTIPQLYY124 pKa = 8.9HH125 pKa = 6.58HH126 pKa = 6.36QLQSCSVAQQWQVSCINLCHH146 pKa = 6.1IHH148 pKa = 6.49SFNPYY153 pKa = 9.6LL154 pKa = 4.15

Molecular weight: 17.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2772

154

2075

693.0

76.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.225 ± 0.733

1.876 ± 0.436

4.365 ± 0.938

2.417 ± 0.41

5.556 ± 0.823

6.746 ± 0.787

3.319 ± 0.409

5.375 ± 0.632

2.85 ± 0.415

10.281 ± 1.14

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.588

3.896 ± 0.253

7.612 ± 0.477

4.113 ± 0.412

4.834 ± 0.508

7.648 ± 0.484

5.844 ± 0.89

7.179 ± 0.474

2.309 ± 0.447

3.427 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski