Halorubrum sp. JWXQ-INN 858

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halorubrum; unclassified Halorubrum

Average proteome isoelectric point is 4.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G2HF61|A0A6G2HF61_9EURY Uncharacterized protein OS=Halorubrum sp. JWXQ-INN 858 OX=2690782 GN=GRS48_07690 PE=4 SV=1
MM1 pKa = 7.64NDD3 pKa = 2.9TTRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84FLVGTTAAGVAALAGCADD26 pKa = 4.78DD27 pKa = 5.18EE28 pKa = 5.69DD29 pKa = 5.41PDD31 pKa = 4.09EE32 pKa = 4.83TPADD36 pKa = 4.01APEE39 pKa = 5.28DD40 pKa = 4.31DD41 pKa = 4.4PDD43 pKa = 4.07EE44 pKa = 4.47PTEE47 pKa = 4.25AADD50 pKa = 5.08DD51 pKa = 4.3APADD55 pKa = 4.05DD56 pKa = 5.37EE57 pKa = 4.46PAEE60 pKa = 4.16SEE62 pKa = 3.92PTYY65 pKa = 9.87EE66 pKa = 4.72IWGADD71 pKa = 3.0QGTNTIYY78 pKa = 10.36IHH80 pKa = 7.25RR81 pKa = 11.84PTDD84 pKa = 3.64DD85 pKa = 3.67GGFEE89 pKa = 4.13QVDD92 pKa = 4.43EE93 pKa = 4.7IDD95 pKa = 3.31TGAVGGEE102 pKa = 4.13VPHH105 pKa = 6.1MVDD108 pKa = 3.05YY109 pKa = 10.75TSDD112 pKa = 3.48YY113 pKa = 10.35EE114 pKa = 4.34YY115 pKa = 11.31AAVACTVGGRR125 pKa = 11.84TLIYY129 pKa = 9.18RR130 pKa = 11.84TEE132 pKa = 3.98DD133 pKa = 3.32RR134 pKa = 11.84EE135 pKa = 4.2LVANLEE141 pKa = 4.42TGAGSHH147 pKa = 6.4FAGFSPDD154 pKa = 3.5DD155 pKa = 4.12EE156 pKa = 4.64YY157 pKa = 12.16VHH159 pKa = 7.58VDD161 pKa = 3.4VIGEE165 pKa = 4.07EE166 pKa = 4.09KK167 pKa = 10.06IVRR170 pKa = 11.84IDD172 pKa = 3.54ADD174 pKa = 3.91LEE176 pKa = 4.3NEE178 pKa = 4.13TFEE181 pKa = 4.22QVAEE185 pKa = 3.87IDD187 pKa = 3.6VRR189 pKa = 11.84EE190 pKa = 4.61DD191 pKa = 3.2EE192 pKa = 4.84TIVDD196 pKa = 3.92AGIEE200 pKa = 4.17TGSPICHH207 pKa = 6.34QYY209 pKa = 10.47AQDD212 pKa = 3.42GRR214 pKa = 11.84SLHH217 pKa = 5.63TLGPSYY223 pKa = 10.9HH224 pKa = 7.23DD225 pKa = 3.68AALVIVDD232 pKa = 4.6HH233 pKa = 7.88DD234 pKa = 4.25DD235 pKa = 3.84FSVDD239 pKa = 2.76RR240 pKa = 11.84AYY242 pKa = 10.02PGEE245 pKa = 4.23EE246 pKa = 3.98VPTNCGTVSHH256 pKa = 6.75PTDD259 pKa = 3.03EE260 pKa = 4.62KK261 pKa = 11.05FYY263 pKa = 9.94LTAGLPSDD271 pKa = 4.35PDD273 pKa = 3.65ADD275 pKa = 3.77PEE277 pKa = 4.28EE278 pKa = 4.55EE279 pKa = 4.25PEE281 pKa = 3.99AAAGVGDD288 pKa = 4.15FYY290 pKa = 11.75VYY292 pKa = 9.64DD293 pKa = 3.78TADD296 pKa = 3.85DD297 pKa = 3.83EE298 pKa = 4.83VATVGSTGGVDD309 pKa = 3.51AHH311 pKa = 6.42GFWFTPDD318 pKa = 4.14GEE320 pKa = 4.56EE321 pKa = 3.7LWVLNRR327 pKa = 11.84EE328 pKa = 4.32TNDD331 pKa = 3.92GVILDD336 pKa = 4.44PGTDD340 pKa = 3.33EE341 pKa = 4.97VVDD344 pKa = 4.93EE345 pKa = 4.67IDD347 pKa = 3.43AFGPEE352 pKa = 4.0QHH354 pKa = 7.47DD355 pKa = 4.71DD356 pKa = 3.78PTEE359 pKa = 4.3SDD361 pKa = 4.04APDD364 pKa = 3.65IMWASPDD371 pKa = 3.53GEE373 pKa = 4.65YY374 pKa = 10.36MFVTLRR380 pKa = 11.84GPNPLSGDD388 pKa = 3.32PHH390 pKa = 6.9AATGINPGYY399 pKa = 10.88AVFDD403 pKa = 3.7VEE405 pKa = 4.3SRR407 pKa = 11.84EE408 pKa = 4.24RR409 pKa = 11.84VDD411 pKa = 5.28LVQPDD416 pKa = 3.89GDD418 pKa = 4.03NPDD421 pKa = 3.65SDD423 pKa = 3.79FHH425 pKa = 8.23GIGVRR430 pKa = 11.84PLTDD434 pKa = 3.12HH435 pKa = 7.64DD436 pKa = 4.93GFTSPPYY443 pKa = 10.63

Molecular weight: 47.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G2HI32|A0A6G2HI32_9EURY Uncharacterized protein OS=Halorubrum sp. JWXQ-INN 858 OX=2690782 GN=GRS48_11730 PE=4 SV=1
MM1 pKa = 7.14ATCDD5 pKa = 3.29VCGEE9 pKa = 4.29YY10 pKa = 10.86EE11 pKa = 4.06NLPYY15 pKa = 10.07QCRR18 pKa = 11.84RR19 pKa = 11.84CGRR22 pKa = 11.84TFCGEE27 pKa = 3.37HH28 pKa = 6.74RR29 pKa = 11.84LPEE32 pKa = 4.09NHH34 pKa = 7.34DD35 pKa = 3.83CPGLRR40 pKa = 11.84GSNDD44 pKa = 3.14PGAVFEE50 pKa = 4.58STVGGVDD57 pKa = 2.85RR58 pKa = 11.84GGVATGTGRR67 pKa = 11.84SSDD70 pKa = 3.24AGIVDD75 pKa = 3.7RR76 pKa = 11.84VKK78 pKa = 10.7RR79 pKa = 11.84RR80 pKa = 11.84VDD82 pKa = 3.25RR83 pKa = 11.84EE84 pKa = 4.05TGRR87 pKa = 11.84GNVMSRR93 pKa = 11.84FRR95 pKa = 11.84GKK97 pKa = 8.89ATYY100 pKa = 10.61VLLAAMWFTLLGQWAALAVGGSALHH125 pKa = 6.06NRR127 pKa = 11.84LFVLEE132 pKa = 5.97SYY134 pKa = 11.01ALWQVWTWGTAVFAHH149 pKa = 7.04DD150 pKa = 4.64PGLLFHH156 pKa = 7.39IIGNSIVLFFFGPLVEE172 pKa = 4.26RR173 pKa = 11.84AVGSKK178 pKa = 10.3RR179 pKa = 11.84FVGFFLVAGVLAGLGHH195 pKa = 5.96VLFVLATGGVVGVLGASGATFAILGVLTVWRR226 pKa = 11.84PNMQILLFFVVPMKK240 pKa = 10.72LKK242 pKa = 10.16YY243 pKa = 8.95LTWGIAAVSAFLVVSTGAAGVGGIAHH269 pKa = 7.91LAHH272 pKa = 7.35LIGFAIGLAFGKK284 pKa = 10.58RR285 pKa = 11.84NVNLARR291 pKa = 11.84RR292 pKa = 11.84TGGVGGVRR300 pKa = 11.84ASGVGGPGGPRR311 pKa = 11.84GPGGPGGRR319 pKa = 11.84FF320 pKa = 3.04

Molecular weight: 33.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2889

0

2889

872988

27

2830

302.2

32.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.108 ± 0.067

0.662 ± 0.015

9.07 ± 0.064

7.842 ± 0.061

3.216 ± 0.025

9.302 ± 0.053

1.961 ± 0.019

3.768 ± 0.034

1.57 ± 0.029

8.612 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.618 ± 0.021

2.044 ± 0.023

4.879 ± 0.028

1.796 ± 0.024

7.035 ± 0.048

4.999 ± 0.031

6.249 ± 0.041

9.717 ± 0.055

1.053 ± 0.015

2.499 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski