Lake Sarah-associated circular virus-13

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA64|A0A126GA64_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-13 OX=1685739 PE=4 SV=1
MM1 pKa = 7.3NSKK4 pKa = 10.7SIIDD8 pKa = 4.02NNDD11 pKa = 2.28KK12 pKa = 9.21STRR15 pKa = 11.84WGFTAFEE22 pKa = 4.35SQWDD26 pKa = 3.94LFKK29 pKa = 10.84TIASPVAEE37 pKa = 4.34WGCHH41 pKa = 5.23EE42 pKa = 4.39EE43 pKa = 4.84LCPTSNRR50 pKa = 11.84LHH52 pKa = 5.07YY53 pKa = 9.97QGYY56 pKa = 9.73IRR58 pKa = 11.84TQSQVRR64 pKa = 11.84FSAMKK69 pKa = 10.03DD70 pKa = 3.16RR71 pKa = 11.84FPGVHH76 pKa = 6.79FLIPDD81 pKa = 3.22NWSAWLNYY89 pKa = 8.78CKK91 pKa = 10.47KK92 pKa = 10.27SATRR96 pKa = 11.84NGDD99 pKa = 3.22GNQVHH104 pKa = 5.96EE105 pKa = 4.44VAKK108 pKa = 10.13NRR110 pKa = 11.84HH111 pKa = 4.55MTMADD116 pKa = 3.15ALTALVPYY124 pKa = 10.18RR125 pKa = 11.84VTEE128 pKa = 4.03KK129 pKa = 10.88EE130 pKa = 3.28IDD132 pKa = 3.98DD133 pKa = 4.4YY134 pKa = 10.37FTQYY138 pKa = 10.53KK139 pKa = 9.84RR140 pKa = 11.84IYY142 pKa = 9.65DD143 pKa = 3.43IKK145 pKa = 9.98DD146 pKa = 3.45QYY148 pKa = 7.78WTAVNRR154 pKa = 11.84YY155 pKa = 8.67ISEE158 pKa = 4.14TKK160 pKa = 9.91NYY162 pKa = 10.04EE163 pKa = 4.3SIGLFTNAQMVVGWAKK179 pKa = 9.57TKK181 pKa = 10.56KK182 pKa = 9.95VWIDD186 pKa = 3.2RR187 pKa = 11.84QTDD190 pKa = 2.84IDD192 pKa = 4.05RR193 pKa = 11.84AGLEE197 pKa = 3.99QAEE200 pKa = 4.21ALQGGTEE207 pKa = 4.25EE208 pKa = 4.8PPAEE212 pKa = 4.69PGNSS216 pKa = 3.22

Molecular weight: 24.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA64|A0A126GA64_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-13 OX=1685739 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.54GGGKK6 pKa = 9.12GRR8 pKa = 11.84KK9 pKa = 9.01GGAKK13 pKa = 9.07GRR15 pKa = 11.84KK16 pKa = 7.13GKK18 pKa = 10.26KK19 pKa = 8.95YY20 pKa = 9.97PISKK24 pKa = 10.33GLGGVPDD31 pKa = 3.81VASLTEE37 pKa = 4.3VISNKK42 pKa = 9.86AQTGAFYY49 pKa = 9.12NTNNSYY55 pKa = 11.12RR56 pKa = 11.84LYY58 pKa = 10.77DD59 pKa = 3.94LSLNSCPRR67 pKa = 11.84ATLVGQAYY75 pKa = 9.55QEE77 pKa = 3.82FRR79 pKa = 11.84MRR81 pKa = 11.84RR82 pKa = 11.84ITLVYY87 pKa = 9.22KK88 pKa = 7.89TTTDD92 pKa = 3.11SFIGGNYY99 pKa = 9.23AVPNLYY105 pKa = 11.25YY106 pKa = 10.38MIDD109 pKa = 3.38KK110 pKa = 10.61KK111 pKa = 11.22GAVPANFTVDD121 pKa = 3.05TLAQMGAILRR131 pKa = 11.84RR132 pKa = 11.84IDD134 pKa = 3.68DD135 pKa = 3.72KK136 pKa = 11.09TIKK139 pKa = 9.66VQWAPAVLQASLTDD153 pKa = 3.68PTALSVGVASAQISPWLPTNNSPGAVGLFQASDD186 pKa = 3.3VDD188 pKa = 4.02HH189 pKa = 7.22FGLSWIVYY197 pKa = 8.12VPVGATSVPYY207 pKa = 10.6DD208 pKa = 3.22VDD210 pKa = 3.26IFVDD214 pKa = 4.22FEE216 pKa = 4.13FRR218 pKa = 11.84KK219 pKa = 9.87PRR221 pKa = 11.84WNTPEE226 pKa = 4.14APPPGSLALNWNQRR240 pKa = 11.84AEE242 pKa = 4.44SQPSTASPTEE252 pKa = 3.74

Molecular weight: 27.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

468

216

252

234.0

26.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.761 ± 0.586

0.855 ± 0.352

5.769 ± 0.469

4.274 ± 1.454

3.632 ± 0.047

7.479 ± 1.266

1.282 ± 0.68

4.915 ± 0.422

6.197 ± 0.188

5.983 ± 0.891

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.258

5.556 ± 0.305

5.769 ± 1.36

4.487 ± 0.399

5.128 ± 0.281

6.838 ± 0.539

7.265 ± 0.094

6.624 ± 1.008

2.778 ± 0.61

4.487 ± 0.094
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski