Hubei virga-like virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJT5|A0A1L3KJT5_9VIRU Uncharacterized protein OS=Hubei virga-like virus 18 OX=1923333 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84LIIFVLFLFFLYY14 pKa = 10.21IPVHH18 pKa = 6.61SYY20 pKa = 6.78TTNPTSLRR28 pKa = 11.84QVCCQCRR35 pKa = 11.84DD36 pKa = 3.18RR37 pKa = 11.84SLTCNFCTQYY47 pKa = 10.68TDD49 pKa = 3.55SHH51 pKa = 6.95GCDD54 pKa = 3.11LSGSQLTTWAISVSTYY70 pKa = 10.67FEE72 pKa = 4.37TNLIPTHH79 pKa = 6.23TSTFASSYY87 pKa = 10.22GYY89 pKa = 10.95LYY91 pKa = 10.37FLKK94 pKa = 10.62DD95 pKa = 3.59SIVTSFAAAAPSCSRR110 pKa = 11.84HH111 pKa = 5.45TNTAQYY117 pKa = 10.81LYY119 pKa = 10.64GDD121 pKa = 5.24SILKK125 pKa = 10.58DD126 pKa = 3.89YY127 pKa = 10.63DD128 pKa = 3.71CSSATVGSSCGVGRR142 pKa = 11.84YY143 pKa = 8.74CSSAITTTSGDD154 pKa = 3.0THH156 pKa = 7.19YY157 pKa = 11.09CFSQYY162 pKa = 11.39AKK164 pKa = 10.32LPSIEE169 pKa = 4.26WYY171 pKa = 10.22DD172 pKa = 3.13INYY175 pKa = 9.89YY176 pKa = 8.78YY177 pKa = 11.22NKK179 pKa = 9.82LASPTGQCVEE189 pKa = 4.09ANYY192 pKa = 10.05PSLLSAMKK200 pKa = 9.67EE201 pKa = 4.01LSSAPASSVTVNGYY215 pKa = 10.75DD216 pKa = 3.61DD217 pKa = 3.95TSMQISVRR225 pKa = 11.84STDD228 pKa = 3.5YY229 pKa = 11.53LLNTYY234 pKa = 10.74RR235 pKa = 11.84MLEE238 pKa = 3.99RR239 pKa = 11.84GLPFAKK245 pKa = 9.04RR246 pKa = 11.84TYY248 pKa = 10.96QNIEE252 pKa = 4.27TYY254 pKa = 9.39CTDD257 pKa = 3.55DD258 pKa = 3.5PVVTSRR264 pKa = 11.84IFSSADD270 pKa = 3.22QMYY273 pKa = 8.2TWYY276 pKa = 11.09LNVLVIRR283 pKa = 11.84RR284 pKa = 11.84GPCSLYY290 pKa = 10.55VPYY293 pKa = 10.79SEE295 pKa = 5.65AQDD298 pKa = 3.19ICADD302 pKa = 3.9DD303 pKa = 5.43LLISDD308 pKa = 4.54YY309 pKa = 10.47TASCPDD315 pKa = 3.18SFRR318 pKa = 11.84PRR320 pKa = 11.84QFSPNRR326 pKa = 11.84WLDD329 pKa = 3.53YY330 pKa = 10.99NITVPNPNSTCDD342 pKa = 3.09KK343 pKa = 9.48MKK345 pKa = 10.67EE346 pKa = 3.92LFEE349 pKa = 5.4CGVKK353 pKa = 10.37DD354 pKa = 3.83WIDD357 pKa = 3.36YY358 pKa = 7.9FANLTAGGGSGGSANYY374 pKa = 9.54EE375 pKa = 3.61EE376 pKa = 5.03HH377 pKa = 7.36ARR379 pKa = 11.84LTDD382 pKa = 3.67EE383 pKa = 4.25YY384 pKa = 11.27AQARR388 pKa = 11.84QFGWFSWISSVFEE401 pKa = 4.4PGIKK405 pKa = 10.27LIFDD409 pKa = 3.82IFGSNFEE416 pKa = 4.85DD417 pKa = 4.17YY418 pKa = 11.19VVTFFEE424 pKa = 4.95KK425 pKa = 10.43LLEE428 pKa = 4.46YY429 pKa = 10.19ILQIVFEE436 pKa = 4.81LFNSIVALFKK446 pKa = 10.7KK447 pKa = 10.0SQQFIDD453 pKa = 3.65KK454 pKa = 9.91LVTFITRR461 pKa = 11.84ILDD464 pKa = 3.39VLFSFIAFLLKK475 pKa = 10.73ALIGVVLKK483 pKa = 10.7IEE485 pKa = 3.78QHH487 pKa = 5.59YY488 pKa = 11.14LLFEE492 pKa = 4.16YY493 pKa = 10.83VLLFLLVDD501 pKa = 3.88YY502 pKa = 10.81YY503 pKa = 11.34LINNNIFSLLVVLLVMVVVGIDD525 pKa = 3.18RR526 pKa = 11.84RR527 pKa = 11.84SPSILLAFHH536 pKa = 6.27SLEE539 pKa = 3.91YY540 pKa = 10.56AYY542 pKa = 11.36VNLSGYY548 pKa = 10.37DD549 pKa = 3.73PSSLTWDD556 pKa = 3.48YY557 pKa = 11.66SLTYY561 pKa = 10.22HH562 pKa = 6.61SYY564 pKa = 11.11SRR566 pKa = 11.84NKK568 pKa = 8.85TYY570 pKa = 10.77NISFPPLPEE579 pKa = 5.5LPDD582 pKa = 3.34VPLYY586 pKa = 9.01NTSTQVNHH594 pKa = 7.01TYY596 pKa = 10.51PLYY599 pKa = 10.17EE600 pKa = 4.43IKK602 pKa = 10.71NHH604 pKa = 5.75TVDD607 pKa = 3.63CDD609 pKa = 3.75SYY611 pKa = 11.23PLYY614 pKa = 9.84NTSSYY619 pKa = 10.65FIHH622 pKa = 6.4QAA624 pKa = 2.95

Molecular weight: 71.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJZ2|A0A1L3KJZ2_9VIRU Uncharacterized protein OS=Hubei virga-like virus 18 OX=1923333 PE=4 SV=1
MM1 pKa = 7.67PNIPASILYY10 pKa = 10.27DD11 pKa = 3.41FVVCSLKK18 pKa = 9.39TQVDD22 pKa = 3.1IAKK25 pKa = 9.9RR26 pKa = 11.84VRR28 pKa = 11.84DD29 pKa = 3.96IQNADD34 pKa = 3.86YY35 pKa = 11.1ILFDD39 pKa = 3.78PSFRR43 pKa = 11.84RR44 pKa = 11.84THH46 pKa = 7.43RR47 pKa = 11.84IRR49 pKa = 11.84IGDD52 pKa = 3.48YY53 pKa = 9.89RR54 pKa = 11.84VEE56 pKa = 3.8VDD58 pKa = 3.03AMGRR62 pKa = 11.84KK63 pKa = 8.98AVTCAAAKK71 pKa = 10.03EE72 pKa = 4.3VFDD75 pKa = 5.14TDD77 pKa = 5.17DD78 pKa = 2.95YY79 pKa = 9.14WQIVRR84 pKa = 11.84EE85 pKa = 4.25FDD87 pKa = 3.44CDD89 pKa = 4.82DD90 pKa = 4.69IILHH94 pKa = 5.67HH95 pKa = 6.95HH96 pKa = 6.21CVALRR101 pKa = 11.84AKK103 pKa = 9.71PVVKK107 pKa = 10.38SRR109 pKa = 11.84SKK111 pKa = 10.13LTLDD115 pKa = 3.67IPPPTHH121 pKa = 6.28HH122 pKa = 7.04HH123 pKa = 6.41PASLSDD129 pKa = 3.16ATTPPSSPP137 pKa = 3.3

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3620

137

2424

724.0

82.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.022 ± 0.576

2.127 ± 0.27

6.713 ± 0.742

3.536 ± 0.342

5.497 ± 1.029

4.116 ± 0.381

2.762 ± 0.449

7.21 ± 0.374

4.53 ± 0.747

9.586 ± 0.634

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.602 ± 0.147

5.359 ± 0.556

4.834 ± 0.41

2.901 ± 0.222

5.138 ± 0.844

8.729 ± 1.019

6.547 ± 0.492

6.602 ± 0.501

1.022 ± 0.084

5.166 ± 0.877
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski