Paenibacillus sp. D9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4351 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5R0I0|A0A0F5R0I0_9BACL DNA polymerase III PolC-type OS=Paenibacillus sp. D9 OX=665792 GN=polC PE=3 SV=1
MM1 pKa = 7.43PTFDD5 pKa = 3.66SVQVTGSCTVQGALLVGGNQTVQNDD30 pKa = 3.79LQVDD34 pKa = 4.62GNQTIAQGLQVNGSQTILNHH54 pKa = 6.43LDD56 pKa = 3.47VEE58 pKa = 4.86GSAAIGGTVSVFSRR72 pKa = 11.84LIIDD76 pKa = 4.96ALPSAPGIASSPTAVNYY93 pKa = 10.16YY94 pKa = 10.29SSGSAAQPGLVLPGSDD110 pKa = 2.77GSVYY114 pKa = 11.04VLFVDD119 pKa = 4.34VSGAVPALGIQRR131 pKa = 11.84LL132 pKa = 3.67

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5R5W7|A0A0F5R5W7_9BACL Xylose isomerase OS=Paenibacillus sp. D9 OX=665792 GN=VE23_18160 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVSKK11 pKa = 10.72RR12 pKa = 11.84KK13 pKa = 8.96KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSSKK25 pKa = 10.47NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.91KK37 pKa = 10.37GRR39 pKa = 11.84SVLSAA44 pKa = 3.74

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4351

0

4351

1342033

26

2864

308.4

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.963 ± 0.05

0.716 ± 0.011

5.123 ± 0.03

6.681 ± 0.048

3.856 ± 0.03

8.366 ± 0.04

1.919 ± 0.018

5.831 ± 0.033

4.707 ± 0.037

10.462 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.811 ± 0.016

3.048 ± 0.028

4.376 ± 0.028

3.475 ± 0.023

5.921 ± 0.044

6.575 ± 0.034

4.875 ± 0.036

6.861 ± 0.03

1.267 ± 0.015

3.164 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski