Apis mellifera associated microvirus 12

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UU62|A0A3S8UU62_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 12 OX=2494739 PE=4 SV=1
MM1 pKa = 7.64PKK3 pKa = 10.1LRR5 pKa = 11.84ARR7 pKa = 11.84RR8 pKa = 11.84GLNPLPLSPDD18 pKa = 2.94PMYY21 pKa = 10.85YY22 pKa = 9.45PQVNFGYY29 pKa = 10.75VGDD32 pKa = 3.86EE33 pKa = 4.17SDD35 pKa = 4.25RR36 pKa = 11.84VAHH39 pKa = 6.02EE40 pKa = 4.36CVPPVTAEE48 pKa = 3.72TMTRR52 pKa = 11.84QEE54 pKa = 4.89LGPATDD60 pKa = 3.21VNIIVKK66 pKa = 10.16QYY68 pKa = 10.12GASFYY73 pKa = 11.16ASGDD77 pKa = 3.19ASVPVHH83 pKa = 7.01DD84 pKa = 5.55FSLDD88 pKa = 3.33ATLLAQVRR96 pKa = 11.84RR97 pKa = 11.84EE98 pKa = 4.54ALDD101 pKa = 3.12LWSRR105 pKa = 11.84LSPRR109 pKa = 11.84QRR111 pKa = 11.84EE112 pKa = 4.33IVGSPDD118 pKa = 3.17QMVSLEE124 pKa = 4.1LQGEE128 pKa = 4.52LGAVLDD134 pKa = 4.58AVAEE138 pKa = 4.17ASSAEE143 pKa = 4.11AGGSGSPPADD153 pKa = 3.38GEE155 pKa = 4.33ATRR158 pKa = 11.84SVV160 pKa = 3.29

Molecular weight: 17.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U630|A0A3Q8U630_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 12 OX=2494739 PE=3 SV=1
MM1 pKa = 7.98PLPLIGGLVAGAKK14 pKa = 10.06ALGGAAMKK22 pKa = 9.76AAPWLVPSALGAVGAQRR39 pKa = 11.84TNDD42 pKa = 3.33MSRR45 pKa = 11.84DD46 pKa = 3.47LMRR49 pKa = 11.84EE50 pKa = 3.48NMAWQEE56 pKa = 3.96KK57 pKa = 8.11MANTAKK63 pKa = 10.02QRR65 pKa = 11.84AVKK68 pKa = 10.25DD69 pKa = 3.49LEE71 pKa = 4.31AAGLNPGLAYY81 pKa = 10.24GYY83 pKa = 8.34SAPMGSVSSPAMQDD97 pKa = 3.2EE98 pKa = 4.43IGAGVANAQQARR110 pKa = 11.84ATNVQLQAQRR120 pKa = 11.84ASTQKK125 pKa = 9.5IAAEE129 pKa = 3.87ARR131 pKa = 11.84IMEE134 pKa = 4.4AQAKK138 pKa = 9.31VEE140 pKa = 3.98EE141 pKa = 4.3RR142 pKa = 11.84KK143 pKa = 9.86AAEE146 pKa = 3.94FLDD149 pKa = 4.11PGVSTEE155 pKa = 4.43RR156 pKa = 11.84LNATYY161 pKa = 10.84SKK163 pKa = 10.79LRR165 pKa = 11.84AEE167 pKa = 4.46GLLGVEE173 pKa = 4.91ALRR176 pKa = 11.84QRR178 pKa = 11.84DD179 pKa = 3.56EE180 pKa = 4.16ALQTQRR186 pKa = 11.84EE187 pKa = 4.18RR188 pKa = 11.84LRR190 pKa = 11.84AIGLSNQQIEE200 pKa = 4.16QVIRR204 pKa = 11.84SGAASEE210 pKa = 4.18EE211 pKa = 4.14QTRR214 pKa = 11.84QAIAFMRR221 pKa = 11.84TMQPMEE227 pKa = 4.25VEE229 pKa = 4.27MMRR232 pKa = 11.84LRR234 pKa = 11.84QLMQKK239 pKa = 10.71LNIDD243 pKa = 3.49DD244 pKa = 3.68TRR246 pKa = 11.84YY247 pKa = 9.77GLSRR251 pKa = 11.84SRR253 pKa = 11.84AEE255 pKa = 3.55SDD257 pKa = 3.44YY258 pKa = 10.83FDD260 pKa = 4.1AVGGLGVGAEE270 pKa = 3.98RR271 pKa = 11.84LGVSRR276 pKa = 11.84LVGDD280 pKa = 4.27ALSSVTPLGRR290 pKa = 11.84MRR292 pKa = 11.84VRR294 pKa = 11.84QGAQSIKK301 pKa = 9.7EE302 pKa = 3.9SRR304 pKa = 11.84SRR306 pKa = 11.84VGAFNARR313 pKa = 11.84RR314 pKa = 11.84DD315 pKa = 3.43AEE317 pKa = 4.25AARR320 pKa = 11.84KK321 pKa = 8.84AWLEE325 pKa = 4.23SITPKK330 pKa = 10.49PP331 pKa = 3.4

Molecular weight: 35.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1445

96

545

289.0

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.588 ± 1.762

0.761 ± 0.444

5.052 ± 0.712

5.19 ± 0.996

3.253 ± 1.005

8.374 ± 0.428

2.145 ± 0.794

3.668 ± 0.578

1.938 ± 0.589

8.097 ± 0.393

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.681

2.63 ± 0.803

6.505 ± 0.995

5.26 ± 0.682

8.789 ± 1.052

7.543 ± 0.837

5.606 ± 1.054

7.405 ± 0.964

1.799 ± 0.425

2.699 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski