Thiobacimonas phage vB_ThpS-P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0T6D1|A0A1B0T6D1_9CAUD Tail component OS=Thiobacimonas phage vB_ThpS-P1 OX=1698523 GN=vBThpSP1_003 PE=4 SV=1
MM1 pKa = 7.95ADD3 pKa = 3.17GGVPTTEE10 pKa = 4.03LGEE13 pKa = 4.26VAGAEE18 pKa = 3.82AVLANYY24 pKa = 8.8EE25 pKa = 4.24GTTGLLSTSALASILAAAGVLAPVTVTDD53 pKa = 3.9ALGTRR58 pKa = 11.84VTALEE63 pKa = 4.16GTVIAGGSPTAGGPVAVVATGNVTLSGEE91 pKa = 4.06QTIDD95 pKa = 3.16GVTTSASRR103 pKa = 11.84VLLVGQTDD111 pKa = 3.65PAEE114 pKa = 4.06NGIWVTASGAWTRR127 pKa = 11.84ATDD130 pKa = 3.45MDD132 pKa = 4.15EE133 pKa = 4.15GAEE136 pKa = 4.09VQRR139 pKa = 11.84TYY141 pKa = 11.51VYY143 pKa = 9.68VTGGTVGRR151 pKa = 11.84GRR153 pKa = 11.84TYY155 pKa = 9.38FTGSEE160 pKa = 4.12VTTIGTDD167 pKa = 3.33DD168 pKa = 3.23IVFTEE173 pKa = 4.49FQNGNFLLTEE183 pKa = 4.72LDD185 pKa = 3.8AKK187 pKa = 11.09LDD189 pKa = 4.23DD190 pKa = 5.18ADD192 pKa = 4.63LLQSVTIAAGGTVEE206 pKa = 4.42GMANIFAAEE215 pKa = 3.68PDD217 pKa = 3.74MRR219 pKa = 11.84NGATWGDD226 pKa = 3.51VSQADD231 pKa = 4.34RR232 pKa = 11.84YY233 pKa = 11.19DD234 pKa = 3.11IGTFRR239 pKa = 11.84AAEE242 pKa = 4.16KK243 pKa = 10.51GSTGSEE249 pKa = 3.7HH250 pKa = 6.84AVYY253 pKa = 9.55TGTSATTTDD262 pKa = 2.63TWFFGAFIVSTPDD275 pKa = 3.4GVSMPSATEE284 pKa = 3.92PAPGTQTEE292 pKa = 4.12ASGTGSFSAISFASTGDD309 pKa = 2.97GGYY312 pKa = 9.89IDD314 pKa = 5.71LSDD317 pKa = 4.1DD318 pKa = 4.0ARR320 pKa = 11.84LYY322 pKa = 8.92WCYY325 pKa = 10.89GYY327 pKa = 10.28IPAGSSDD334 pKa = 4.1QICLGLASVNAGTLMSWFDD353 pKa = 4.13LRR355 pKa = 11.84LMAAEE360 pKa = 4.7PDD362 pKa = 3.68ASTVTALTQALAGAWAEE379 pKa = 4.43GVRR382 pKa = 11.84SAPLLTQASIDD393 pKa = 3.81EE394 pKa = 4.72TVLPTPITIASGGTPDD410 pKa = 5.42GYY412 pKa = 11.28DD413 pKa = 3.13NQLAGEE419 pKa = 4.19PEE421 pKa = 4.0MRR423 pKa = 11.84AEE425 pKa = 4.23ATWQTVEE432 pKa = 4.14PRR434 pKa = 11.84YY435 pKa = 9.77QSRR438 pKa = 11.84TGATRR443 pKa = 11.84AARR446 pKa = 11.84KK447 pKa = 6.9TATSSEE453 pKa = 4.22YY454 pKa = 10.85AIFSADD460 pKa = 3.22AATTTDD466 pKa = 2.49TWFFCACIVAVPPGVAMTSATVPNPGTQTDD496 pKa = 3.83GSGFAFISTSSGDD509 pKa = 3.17SGYY512 pKa = 11.08VDD514 pKa = 3.54LAEE517 pKa = 4.21GVRR520 pKa = 11.84LFWAYY525 pKa = 11.1SYY527 pKa = 10.95IPSNTTNQICVGVTGLNAGSFIGSFDD553 pKa = 3.77LRR555 pKa = 11.84LMDD558 pKa = 4.09VEE560 pKa = 4.55PSKK563 pKa = 10.8STITALTQAAAGRR576 pKa = 11.84WANGVKK582 pKa = 9.38MAEE585 pKa = 4.04VLGAQVKK592 pKa = 9.28RR593 pKa = 11.84QPTVRR598 pKa = 11.84GALSVTSAIAAVIRR612 pKa = 11.84PSKK615 pKa = 10.62LFARR619 pKa = 11.84LNPPKK624 pKa = 10.1VHH626 pKa = 6.56KK627 pKa = 10.11PSAGTIYY634 pKa = 10.5VAAPDD639 pKa = 4.09AAFLPAGSDD648 pKa = 3.29ADD650 pKa = 3.79GDD652 pKa = 4.22GSRR655 pKa = 11.84EE656 pKa = 3.89APYY659 pKa = 9.41ATLTQALSVVPSTGDD674 pKa = 3.45YY675 pKa = 11.17LILCDD680 pKa = 5.33GSFAEE685 pKa = 4.83DD686 pKa = 3.13NAGRR690 pKa = 11.84FILSGEE696 pKa = 3.82FDD698 pKa = 4.0LPVVFRR704 pKa = 11.84PYY706 pKa = 10.87VEE708 pKa = 4.82GSASITNASGTNGVINVRR726 pKa = 11.84SAAASNIQFDD736 pKa = 4.42GLTILPSTDD745 pKa = 2.64GCTTIWVNPSAGTALASNILFFNCRR770 pKa = 11.84LVQRR774 pKa = 11.84AYY776 pKa = 9.77TSAAHH781 pKa = 7.41LIRR784 pKa = 11.84FEE786 pKa = 3.77ADD788 pKa = 3.36YY789 pKa = 11.0AVKK792 pKa = 10.37GVGFVGCALVGLSGVGSTPPNIILTPANTAQVHH825 pKa = 4.66EE826 pKa = 5.35RR827 pKa = 11.84IGFWDD832 pKa = 3.94CWTEE836 pKa = 4.21GEE838 pKa = 4.31WGAFSYY844 pKa = 10.85RR845 pKa = 11.84FTGTKK850 pKa = 10.12AVTVVGNDD858 pKa = 3.05FKK860 pKa = 11.57AVVNHH865 pKa = 6.65ALLLGTDD872 pKa = 4.05SSTLSPPAISDD883 pKa = 3.58ALVIDD888 pKa = 4.21NDD890 pKa = 3.93FLAQGSNPHH899 pKa = 5.98GLEE902 pKa = 3.36IGEE905 pKa = 4.27NVSGIAALNRR915 pKa = 11.84VTSGLQGIVVKK926 pKa = 9.93GTDD929 pKa = 3.42DD930 pKa = 3.35VDD932 pKa = 4.28IIEE935 pKa = 4.3NTVRR939 pKa = 11.84LVPTAANGSALYY951 pKa = 9.61PKK953 pKa = 10.41AATNTRR959 pKa = 11.84WVGNAVIIEE968 pKa = 4.18DD969 pKa = 4.19TGLLAYY975 pKa = 9.61GFNEE979 pKa = 4.8GPDD982 pKa = 3.81GANLSGDD989 pKa = 3.47NTLSDD994 pKa = 4.07TYY996 pKa = 11.33FEE998 pKa = 4.1IHH1000 pKa = 6.21GAASQALLWSGASGSTGGATSDD1022 pKa = 3.29GNEE1025 pKa = 3.96YY1026 pKa = 11.03VLDD1029 pKa = 4.41GASLGAVRR1037 pKa = 11.84GVTVTDD1043 pKa = 3.87LAEE1046 pKa = 4.44LQAAWASDD1054 pKa = 3.39GLTGDD1059 pKa = 4.05DD1060 pKa = 4.05TANDD1064 pKa = 3.71DD1065 pKa = 4.01TSTVSAWW1072 pKa = 3.02

Molecular weight: 110.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0T6H5|A0A1B0T6H5_9CAUD Cro/CI family transcriptional regulator OS=Thiobacimonas phage vB_ThpS-P1 OX=1698523 GN=vBThpSP1_048 PE=4 SV=1
MM1 pKa = 7.28IHH3 pKa = 6.33ATPHH7 pKa = 5.41AVRR10 pKa = 11.84RR11 pKa = 11.84PGPLICPADD20 pKa = 3.8VKK22 pKa = 10.93HH23 pKa = 5.94YY24 pKa = 9.88AAQVSRR30 pKa = 11.84GLPAEE35 pKa = 3.77QRR37 pKa = 11.84AAVAVEE43 pKa = 4.43LIRR46 pKa = 11.84SISEE50 pKa = 3.97PEE52 pKa = 3.91SFLVVRR58 pKa = 11.84DD59 pKa = 3.38AARR62 pKa = 11.84IVQGHH67 pKa = 5.92GNALLSDD74 pKa = 3.95AMRR77 pKa = 11.84VVEE80 pKa = 4.54GQTEE84 pKa = 4.34HH85 pKa = 5.44TTVASLTDD93 pKa = 3.27QSATVSPVARR103 pKa = 11.84GTAGTPRR110 pKa = 11.84SRR112 pKa = 11.84RR113 pKa = 11.84LSPLSVAWARR123 pKa = 11.84FRR125 pKa = 11.84AAEE128 pKa = 4.1RR129 pKa = 11.84RR130 pKa = 11.84FSDD133 pKa = 5.1SIWGDD138 pKa = 3.06ALAGVFALGALFGLSIILWGLQQ160 pKa = 3.05

Molecular weight: 17.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12234

46

1116

235.3

25.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.563 ± 0.425

0.785 ± 0.12

6.22 ± 0.245

7.021 ± 0.318

3.18 ± 0.161

8.452 ± 0.413

1.749 ± 0.2

4.733 ± 0.164

3.18 ± 0.313

8.738 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.209

2.379 ± 0.203

4.921 ± 0.232

3.245 ± 0.261

7.716 ± 0.461

5.91 ± 0.333

6.498 ± 0.531

6.523 ± 0.317

1.831 ± 0.118

2.06 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski