Amino acid dipepetide frequency for Enterobacter phage Tyrion

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.472AlaAla: 12.472 ± 1.517
0.462AlaCys: 0.462 ± 0.193
5.697AlaAsp: 5.697 ± 0.694
5.466AlaGlu: 5.466 ± 0.817
3.541AlaPhe: 3.541 ± 0.484
7.083AlaGly: 7.083 ± 0.73
1.155AlaHis: 1.155 ± 0.307
4.234AlaIle: 4.234 ± 0.527
5.081AlaLys: 5.081 ± 0.637
8.546AlaLeu: 8.546 ± 0.685
3.926AlaMet: 3.926 ± 0.577
4.08AlaAsn: 4.08 ± 0.502
2.772AlaPro: 2.772 ± 0.492
5.774AlaGln: 5.774 ± 0.896
5.235AlaArg: 5.235 ± 0.784
6.082AlaSer: 6.082 ± 0.586
4.619AlaThr: 4.619 ± 0.525
7.006AlaVal: 7.006 ± 0.745
1.925AlaTrp: 1.925 ± 0.383
3.387AlaTyr: 3.387 ± 0.671
0.0AlaXaa: 0.0 ± 0.0
Cys
0.847CysAla: 0.847 ± 0.281
0.154CysCys: 0.154 ± 0.107
0.693CysAsp: 0.693 ± 0.189
0.693CysGlu: 0.693 ± 0.214
0.462CysPhe: 0.462 ± 0.141
1.232CysGly: 1.232 ± 0.395
0.0CysHis: 0.0 ± 0.0
0.616CysIle: 0.616 ± 0.241
0.616CysLys: 0.616 ± 0.223
0.847CysLeu: 0.847 ± 0.283
0.539CysMet: 0.539 ± 0.22
0.539CysAsn: 0.539 ± 0.165
0.462CysPro: 0.462 ± 0.202
0.308CysGln: 0.308 ± 0.142
0.539CysArg: 0.539 ± 0.21
0.616CysSer: 0.616 ± 0.277
0.385CysThr: 0.385 ± 0.152
0.462CysVal: 0.462 ± 0.183
0.231CysTrp: 0.231 ± 0.132
0.077CysTyr: 0.077 ± 0.08
0.0CysXaa: 0.0 ± 0.0
Asp
7.006AspAla: 7.006 ± 0.748
0.847AspCys: 0.847 ± 0.255
3.849AspAsp: 3.849 ± 0.555
4.773AspGlu: 4.773 ± 0.806
1.54AspPhe: 1.54 ± 0.373
4.465AspGly: 4.465 ± 0.475
0.924AspHis: 0.924 ± 0.277
3.157AspIle: 3.157 ± 0.479
3.618AspLys: 3.618 ± 0.477
3.772AspLeu: 3.772 ± 0.411
2.464AspMet: 2.464 ± 0.504
3.157AspAsn: 3.157 ± 0.363
2.464AspPro: 2.464 ± 0.472
2.156AspGln: 2.156 ± 0.399
2.849AspArg: 2.849 ± 0.508
4.157AspSer: 4.157 ± 0.519
2.926AspThr: 2.926 ± 0.611
4.234AspVal: 4.234 ± 0.584
1.001AspTrp: 1.001 ± 0.232
1.694AspTyr: 1.694 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
5.235GluAla: 5.235 ± 0.637
0.693GluCys: 0.693 ± 0.21
2.772GluAsp: 2.772 ± 0.547
3.003GluGlu: 3.003 ± 0.591
2.926GluPhe: 2.926 ± 0.466
3.541GluGly: 3.541 ± 0.417
1.078GluHis: 1.078 ± 0.284
3.772GluIle: 3.772 ± 0.494
3.541GluLys: 3.541 ± 0.607
5.081GluLeu: 5.081 ± 0.611
1.617GluMet: 1.617 ± 0.363
2.233GluAsn: 2.233 ± 0.342
1.694GluPro: 1.694 ± 0.316
3.541GluGln: 3.541 ± 0.656
3.695GluArg: 3.695 ± 0.615
3.618GluSer: 3.618 ± 0.619
2.849GluThr: 2.849 ± 0.431
3.618GluVal: 3.618 ± 0.511
0.616GluTrp: 0.616 ± 0.241
2.233GluTyr: 2.233 ± 0.414
0.0GluXaa: 0.0 ± 0.0
Phe
3.003PheAla: 3.003 ± 0.452
0.231PheCys: 0.231 ± 0.122
2.541PheAsp: 2.541 ± 0.527
1.848PheGlu: 1.848 ± 0.392
1.386PhePhe: 1.386 ± 0.4
2.31PheGly: 2.31 ± 0.35
0.462PheHis: 0.462 ± 0.245
2.233PheIle: 2.233 ± 0.333
1.463PheLys: 1.463 ± 0.29
2.233PheLeu: 2.233 ± 0.597
1.001PheMet: 1.001 ± 0.238
2.156PheAsn: 2.156 ± 0.359
1.617PhePro: 1.617 ± 0.377
1.155PheGln: 1.155 ± 0.367
2.464PheArg: 2.464 ± 0.333
2.695PheSer: 2.695 ± 0.355
1.694PheThr: 1.694 ± 0.313
1.925PheVal: 1.925 ± 0.404
0.462PheTrp: 0.462 ± 0.143
1.001PheTyr: 1.001 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
6.005GlyAla: 6.005 ± 0.882
1.001GlyCys: 1.001 ± 0.297
4.234GlyAsp: 4.234 ± 0.636
4.003GlyGlu: 4.003 ± 0.494
2.387GlyPhe: 2.387 ± 0.492
6.39GlyGly: 6.39 ± 0.991
1.617GlyHis: 1.617 ± 0.353
4.696GlyIle: 4.696 ± 0.577
5.389GlyLys: 5.389 ± 0.679
5.851GlyLeu: 5.851 ± 0.647
2.618GlyMet: 2.618 ± 0.386
3.234GlyAsn: 3.234 ± 0.429
1.617GlyPro: 1.617 ± 0.408
3.541GlyGln: 3.541 ± 0.712
3.849GlyArg: 3.849 ± 0.454
4.003GlySer: 4.003 ± 0.557
4.773GlyThr: 4.773 ± 0.544
5.158GlyVal: 5.158 ± 0.849
0.924GlyTrp: 0.924 ± 0.357
3.08GlyTyr: 3.08 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.155HisAla: 1.155 ± 0.338
0.539HisCys: 0.539 ± 0.248
0.77HisAsp: 0.77 ± 0.236
1.232HisGlu: 1.232 ± 0.322
0.77HisPhe: 0.77 ± 0.205
1.155HisGly: 1.155 ± 0.294
0.308HisHis: 0.308 ± 0.14
0.847HisIle: 0.847 ± 0.212
0.847HisLys: 0.847 ± 0.261
1.54HisLeu: 1.54 ± 0.347
0.385HisMet: 0.385 ± 0.166
0.693HisAsn: 0.693 ± 0.272
0.616HisPro: 0.616 ± 0.21
0.539HisGln: 0.539 ± 0.205
0.77HisArg: 0.77 ± 0.233
0.847HisSer: 0.847 ± 0.244
0.77HisThr: 0.77 ± 0.194
1.078HisVal: 1.078 ± 0.347
0.77HisTrp: 0.77 ± 0.258
0.308HisTyr: 0.308 ± 0.162
0.0HisXaa: 0.0 ± 0.0
Ile
6.313IleAla: 6.313 ± 0.767
0.539IleCys: 0.539 ± 0.263
4.542IleAsp: 4.542 ± 0.564
3.157IleGlu: 3.157 ± 0.621
1.54IlePhe: 1.54 ± 0.338
3.618IleGly: 3.618 ± 0.48
1.155IleHis: 1.155 ± 0.281
2.618IleIle: 2.618 ± 0.615
3.08IleLys: 3.08 ± 0.6
3.003IleLeu: 3.003 ± 0.548
0.924IleMet: 0.924 ± 0.275
3.926IleAsn: 3.926 ± 0.626
2.695IlePro: 2.695 ± 0.435
2.233IleGln: 2.233 ± 0.335
3.31IleArg: 3.31 ± 0.581
4.234IleSer: 4.234 ± 0.628
4.157IleThr: 4.157 ± 0.538
3.003IleVal: 3.003 ± 0.893
0.924IleTrp: 0.924 ± 0.23
1.463IleTyr: 1.463 ± 0.361
0.0IleXaa: 0.0 ± 0.0
Lys
4.234LysAla: 4.234 ± 0.528
0.539LysCys: 0.539 ± 0.221
2.464LysAsp: 2.464 ± 0.458
3.08LysGlu: 3.08 ± 0.456
1.54LysPhe: 1.54 ± 0.491
3.387LysGly: 3.387 ± 0.542
1.001LysHis: 1.001 ± 0.329
3.003LysIle: 3.003 ± 0.644
3.31LysLys: 3.31 ± 0.739
5.004LysLeu: 5.004 ± 0.63
1.617LysMet: 1.617 ± 0.421
2.156LysAsn: 2.156 ± 0.465
3.003LysPro: 3.003 ± 0.581
1.848LysGln: 1.848 ± 0.44
3.849LysArg: 3.849 ± 0.575
3.618LysSer: 3.618 ± 0.495
2.695LysThr: 2.695 ± 0.435
2.772LysVal: 2.772 ± 0.519
1.463LysTrp: 1.463 ± 0.337
1.925LysTyr: 1.925 ± 0.531
0.0LysXaa: 0.0 ± 0.0
Leu
7.006LeuAla: 7.006 ± 0.743
0.924LeuCys: 0.924 ± 0.257
4.85LeuAsp: 4.85 ± 0.536
4.08LeuGlu: 4.08 ± 0.532
2.387LeuPhe: 2.387 ± 0.344
4.311LeuGly: 4.311 ± 0.892
1.309LeuHis: 1.309 ± 0.249
4.465LeuIle: 4.465 ± 0.698
4.696LeuLys: 4.696 ± 0.605
7.083LeuLeu: 7.083 ± 0.758
1.925LeuMet: 1.925 ± 0.357
3.695LeuAsn: 3.695 ± 0.545
3.695LeuPro: 3.695 ± 0.558
3.157LeuGln: 3.157 ± 0.419
4.696LeuArg: 4.696 ± 0.634
6.544LeuSer: 6.544 ± 0.751
4.773LeuThr: 4.773 ± 0.556
4.234LeuVal: 4.234 ± 0.488
0.693LeuTrp: 0.693 ± 0.193
2.541LeuTyr: 2.541 ± 0.493
0.0LeuXaa: 0.0 ± 0.0
Met
3.464MetAla: 3.464 ± 0.66
0.308MetCys: 0.308 ± 0.17
1.771MetAsp: 1.771 ± 0.458
1.617MetGlu: 1.617 ± 0.327
1.155MetPhe: 1.155 ± 0.268
2.156MetGly: 2.156 ± 0.405
0.231MetHis: 0.231 ± 0.123
1.232MetIle: 1.232 ± 0.346
1.54MetLys: 1.54 ± 0.45
2.31MetLeu: 2.31 ± 0.466
0.924MetMet: 0.924 ± 0.348
1.848MetAsn: 1.848 ± 0.351
1.463MetPro: 1.463 ± 0.38
0.77MetGln: 0.77 ± 0.277
2.079MetArg: 2.079 ± 0.685
2.387MetSer: 2.387 ± 0.371
2.079MetThr: 2.079 ± 0.373
1.617MetVal: 1.617 ± 0.375
0.385MetTrp: 0.385 ± 0.163
0.924MetTyr: 0.924 ± 0.306
0.0MetXaa: 0.0 ± 0.0
Asn
4.08AsnAla: 4.08 ± 0.65
0.616AsnCys: 0.616 ± 0.238
2.772AsnAsp: 2.772 ± 0.479
2.156AsnGlu: 2.156 ± 0.453
1.309AsnPhe: 1.309 ± 0.227
3.464AsnGly: 3.464 ± 0.593
0.308AsnHis: 0.308 ± 0.144
3.157AsnIle: 3.157 ± 0.522
2.079AsnLys: 2.079 ± 0.427
3.234AsnLeu: 3.234 ± 0.504
1.078AsnMet: 1.078 ± 0.284
2.618AsnAsn: 2.618 ± 0.718
3.08AsnPro: 3.08 ± 0.457
2.772AsnGln: 2.772 ± 0.569
2.772AsnArg: 2.772 ± 0.391
3.157AsnSer: 3.157 ± 0.634
3.387AsnThr: 3.387 ± 0.429
2.695AsnVal: 2.695 ± 0.419
0.77AsnTrp: 0.77 ± 0.316
1.309AsnTyr: 1.309 ± 0.366
0.0AsnXaa: 0.0 ± 0.0
Pro
4.388ProAla: 4.388 ± 0.644
0.231ProCys: 0.231 ± 0.136
3.849ProAsp: 3.849 ± 0.541
3.387ProGlu: 3.387 ± 0.516
1.155ProPhe: 1.155 ± 0.263
3.695ProGly: 3.695 ± 0.397
0.616ProHis: 0.616 ± 0.208
2.233ProIle: 2.233 ± 0.416
1.309ProLys: 1.309 ± 0.365
2.695ProLeu: 2.695 ± 0.418
0.847ProMet: 0.847 ± 0.223
1.155ProAsn: 1.155 ± 0.263
1.694ProPro: 1.694 ± 0.413
2.002ProGln: 2.002 ± 0.387
1.463ProArg: 1.463 ± 0.372
2.926ProSer: 2.926 ± 0.484
1.925ProThr: 1.925 ± 0.361
3.926ProVal: 3.926 ± 0.479
1.078ProTrp: 1.078 ± 0.248
1.001ProTyr: 1.001 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
5.312GlnAla: 5.312 ± 1.022
0.539GlnCys: 0.539 ± 0.224
2.387GlnAsp: 2.387 ± 0.334
2.156GlnGlu: 2.156 ± 0.431
1.848GlnPhe: 1.848 ± 0.353
2.926GlnGly: 2.926 ± 0.478
0.616GlnHis: 0.616 ± 0.264
2.002GlnIle: 2.002 ± 0.47
2.002GlnLys: 2.002 ± 0.361
4.234GlnLeu: 4.234 ± 0.695
1.694GlnMet: 1.694 ± 0.375
1.771GlnAsn: 1.771 ± 0.409
1.771GlnPro: 1.771 ± 0.311
3.849GlnGln: 3.849 ± 0.84
3.464GlnArg: 3.464 ± 0.608
2.772GlnSer: 2.772 ± 0.546
2.772GlnThr: 2.772 ± 0.51
3.387GlnVal: 3.387 ± 0.656
1.078GlnTrp: 1.078 ± 0.288
1.771GlnTyr: 1.771 ± 0.416
0.0GlnXaa: 0.0 ± 0.0
Arg
5.697ArgAla: 5.697 ± 0.664
0.616ArgCys: 0.616 ± 0.242
3.849ArgAsp: 3.849 ± 0.582
4.311ArgGlu: 4.311 ± 0.633
1.54ArgPhe: 1.54 ± 0.226
3.003ArgGly: 3.003 ± 0.378
1.771ArgHis: 1.771 ± 0.412
3.541ArgIle: 3.541 ± 0.494
3.31ArgLys: 3.31 ± 0.411
3.541ArgLeu: 3.541 ± 0.499
1.617ArgMet: 1.617 ± 0.415
3.157ArgAsn: 3.157 ± 0.581
1.309ArgPro: 1.309 ± 0.304
3.772ArgGln: 3.772 ± 0.584
4.003ArgArg: 4.003 ± 0.527
3.234ArgSer: 3.234 ± 0.494
2.772ArgThr: 2.772 ± 0.433
3.695ArgVal: 3.695 ± 0.602
1.694ArgTrp: 1.694 ± 0.342
2.079ArgTyr: 2.079 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
6.698SerAla: 6.698 ± 0.666
0.693SerCys: 0.693 ± 0.293
4.003SerAsp: 4.003 ± 0.457
3.464SerGlu: 3.464 ± 0.525
2.079SerPhe: 2.079 ± 0.336
6.621SerGly: 6.621 ± 0.666
0.616SerHis: 0.616 ± 0.247
3.772SerIle: 3.772 ± 0.53
3.387SerLys: 3.387 ± 0.654
4.619SerLeu: 4.619 ± 0.649
2.156SerMet: 2.156 ± 0.439
3.387SerAsn: 3.387 ± 0.43
3.157SerPro: 3.157 ± 0.504
3.387SerGln: 3.387 ± 0.605
3.618SerArg: 3.618 ± 0.494
4.157SerSer: 4.157 ± 0.71
3.772SerThr: 3.772 ± 0.519
4.465SerVal: 4.465 ± 0.68
0.693SerTrp: 0.693 ± 0.241
1.848SerTyr: 1.848 ± 0.416
0.0SerXaa: 0.0 ± 0.0
Thr
5.774ThrAla: 5.774 ± 0.773
0.462ThrCys: 0.462 ± 0.21
3.31ThrAsp: 3.31 ± 0.443
2.233ThrGlu: 2.233 ± 0.431
2.002ThrPhe: 2.002 ± 0.674
6.313ThrGly: 6.313 ± 0.79
1.155ThrHis: 1.155 ± 0.327
3.234ThrIle: 3.234 ± 0.5
2.156ThrLys: 2.156 ± 0.353
4.619ThrLeu: 4.619 ± 0.603
1.54ThrMet: 1.54 ± 0.329
1.925ThrAsn: 1.925 ± 0.249
3.464ThrPro: 3.464 ± 0.326
2.31ThrGln: 2.31 ± 0.331
3.003ThrArg: 3.003 ± 0.418
3.541ThrSer: 3.541 ± 0.624
3.541ThrThr: 3.541 ± 0.542
3.31ThrVal: 3.31 ± 0.493
1.001ThrTrp: 1.001 ± 0.234
1.771ThrTyr: 1.771 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
5.312ValAla: 5.312 ± 0.617
0.539ValCys: 0.539 ± 0.206
4.234ValAsp: 4.234 ± 0.459
3.849ValGlu: 3.849 ± 0.433
2.156ValPhe: 2.156 ± 0.351
3.926ValGly: 3.926 ± 0.625
0.77ValHis: 0.77 ± 0.203
4.465ValIle: 4.465 ± 0.63
2.772ValLys: 2.772 ± 0.457
4.542ValLeu: 4.542 ± 0.608
2.233ValMet: 2.233 ± 0.472
3.464ValAsn: 3.464 ± 0.503
2.618ValPro: 2.618 ± 0.461
2.695ValGln: 2.695 ± 0.377
4.08ValArg: 4.08 ± 0.455
4.619ValSer: 4.619 ± 0.58
4.311ValThr: 4.311 ± 0.553
4.542ValVal: 4.542 ± 0.708
1.001ValTrp: 1.001 ± 0.278
1.925ValTyr: 1.925 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
1.617TrpAla: 1.617 ± 0.299
0.154TrpCys: 0.154 ± 0.097
0.616TrpAsp: 0.616 ± 0.2
1.001TrpGlu: 1.001 ± 0.263
0.847TrpPhe: 0.847 ± 0.22
1.54TrpGly: 1.54 ± 0.384
0.231TrpHis: 0.231 ± 0.147
0.77TrpIle: 0.77 ± 0.258
1.001TrpLys: 1.001 ± 0.256
1.848TrpLeu: 1.848 ± 0.452
0.539TrpMet: 0.539 ± 0.208
0.539TrpAsn: 0.539 ± 0.185
0.693TrpPro: 0.693 ± 0.264
0.847TrpGln: 0.847 ± 0.221
0.847TrpArg: 0.847 ± 0.242
1.617TrpSer: 1.617 ± 0.367
0.693TrpThr: 0.693 ± 0.231
1.155TrpVal: 1.155 ± 0.263
0.231TrpTrp: 0.231 ± 0.13
0.693TrpTyr: 0.693 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.926TyrAla: 2.926 ± 0.545
0.231TyrCys: 0.231 ± 0.119
1.848TyrAsp: 1.848 ± 0.409
1.848TyrGlu: 1.848 ± 0.315
1.309TyrPhe: 1.309 ± 0.457
3.234TyrGly: 3.234 ± 0.548
0.616TyrHis: 0.616 ± 0.299
2.387TyrIle: 2.387 ± 0.335
1.386TyrLys: 1.386 ± 0.27
2.464TyrLeu: 2.464 ± 0.495
0.539TyrMet: 0.539 ± 0.195
1.078TyrAsn: 1.078 ± 0.28
1.694TyrPro: 1.694 ± 0.456
1.694TyrGln: 1.694 ± 0.364
1.925TyrArg: 1.925 ± 0.382
1.771TyrSer: 1.771 ± 0.342
1.771TyrThr: 1.771 ± 0.399
1.771TyrVal: 1.771 ± 0.381
0.539TyrTrp: 0.539 ± 0.2
1.232TyrTyr: 1.232 ± 0.305
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12990 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski