Galleria mellonella (Greater wax moth)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia;

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J3C177|A0A6J3C177_GALME Endothelin-converting enzyme 1 OS=Galleria mellonella OX=7137 GN=LOC113519472 PE=3 SV=1
MM1 pKa = 7.4EE2 pKa = 5.72RR3 pKa = 11.84NGSEE7 pKa = 3.85RR8 pKa = 11.84DD9 pKa = 3.64VNDD12 pKa = 3.17VEE14 pKa = 4.45EE15 pKa = 4.18WPEE18 pKa = 3.77IYY20 pKa = 10.45EE21 pKa = 4.61DD22 pKa = 4.07ANNMIDD28 pKa = 4.2FDD30 pKa = 5.61FDD32 pKa = 3.94FDD34 pKa = 6.61LINDD38 pKa = 4.35DD39 pKa = 4.56NLSNDD44 pKa = 2.63TWQFFPEE51 pKa = 4.19DD52 pKa = 4.48LEE54 pKa = 5.23NIDD57 pKa = 4.15YY58 pKa = 11.31NSLEE62 pKa = 4.56NIDD65 pKa = 5.4FIPAPEE71 pKa = 4.19TGFSVDD77 pKa = 3.12AVINRR82 pKa = 11.84LEE84 pKa = 5.5DD85 pKa = 2.98ITQGMISLAGDD96 pKa = 3.69FMQCIEE102 pKa = 4.68EE103 pKa = 4.03IRR105 pKa = 11.84VFTAQQFGHH114 pKa = 6.53FEE116 pKa = 3.99QEE118 pKa = 4.13QAWAQQAASVNANGEE133 pKa = 4.18TMRR136 pKa = 11.84CANCNRR142 pKa = 11.84EE143 pKa = 3.99ATAVCSLCRR152 pKa = 11.84RR153 pKa = 11.84TPYY156 pKa = 10.78CSMYY160 pKa = 9.44CQKK163 pKa = 10.39RR164 pKa = 11.84DD165 pKa = 3.27WMSHH169 pKa = 6.61HH170 pKa = 7.1IDD172 pKa = 4.16CLISMAIIEE181 pKa = 5.24ADD183 pKa = 3.41MQQDD187 pKa = 3.05QWYY190 pKa = 9.15GPPPDD195 pKa = 3.99ALL197 pKa = 4.43

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J3C110|A0A6J3C110_GALME zinc finger protein 501-like OS=Galleria mellonella OX=7137 GN=LOC113510080 PE=4 SV=1
MM1 pKa = 6.7QHH3 pKa = 6.94HH4 pKa = 7.11LRR6 pKa = 11.84LLHH9 pKa = 6.98RR10 pKa = 11.84IRR12 pKa = 11.84LRR14 pKa = 11.84GLHH17 pKa = 6.2RR18 pKa = 11.84PALALVGAARR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 5.93RR31 pKa = 11.84GRR33 pKa = 11.84GRR35 pKa = 11.84HH36 pKa = 5.57AGAARR41 pKa = 11.84VADD44 pKa = 4.25GARR47 pKa = 11.84RR48 pKa = 11.84AARR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84ARR55 pKa = 11.84LRR57 pKa = 11.84RR58 pKa = 11.84ARR60 pKa = 11.84PRR62 pKa = 11.84HH63 pKa = 5.71LLLAQRR69 pKa = 11.84AGAGAHH75 pKa = 5.55DD76 pKa = 4.41VRR78 pKa = 11.84RR79 pKa = 11.84AAARR83 pKa = 11.84AAASRR88 pKa = 11.84GRR90 pKa = 11.84GRR92 pKa = 11.84PAAAGAVPRR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84GRR105 pKa = 11.84GGVCARR111 pKa = 11.84PPRR114 pKa = 11.84RR115 pKa = 11.84LHH117 pKa = 6.33AQWPPPAPRR126 pKa = 11.84NAQRR130 pKa = 11.84QLPVPGAVPLRR141 pKa = 11.84RR142 pKa = 11.84VPGVRR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84GRR152 pKa = 11.84GGGVARR158 pKa = 11.84LHH160 pKa = 6.62AGAAVRR166 pKa = 11.84LPVPVGRR173 pKa = 11.84PRR175 pKa = 11.84EE176 pKa = 3.96RR177 pKa = 11.84LPGGVPHH184 pKa = 7.54RR185 pKa = 11.84LQPVAPRR192 pKa = 11.84IPAAVLSHH200 pKa = 6.03RR201 pKa = 11.84TRR203 pKa = 3.89

Molecular weight: 22.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14085

4236

18321

9854621

21

17500

537.9

60.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.881 ± 0.022

1.941 ± 0.029

5.537 ± 0.014

6.75 ± 0.025

3.45 ± 0.014

5.55 ± 0.024

2.507 ± 0.011

5.451 ± 0.016

6.436 ± 0.027

8.917 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.199 ± 0.009

4.994 ± 0.02

5.489 ± 0.026

4.061 ± 0.018

5.634 ± 0.018

7.868 ± 0.026

5.847 ± 0.018

6.204 ± 0.015

1.069 ± 0.006

3.195 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski