Acinetobacter phage vB_AbaP_Alexa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z6F7|A0A6M9Z6F7_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaP_Alexa OX=2736223 GN=Alexa_008 PE=4 SV=1
MM1 pKa = 7.89PKK3 pKa = 10.28PSWEE7 pKa = 3.95NLDD10 pKa = 4.28VFFQTDD16 pKa = 3.54TVGGFGDD23 pKa = 3.59VATFEE28 pKa = 4.49LADD31 pKa = 3.75GSVRR35 pKa = 11.84QITGIFDD42 pKa = 3.59NPYY45 pKa = 10.74LNAQTGEE52 pKa = 4.09YY53 pKa = 9.72EE54 pKa = 4.12IDD56 pKa = 3.44DD57 pKa = 3.79MQPRR61 pKa = 11.84FTCPEE66 pKa = 3.83TAAKK70 pKa = 10.34GIKK73 pKa = 9.88QYY75 pKa = 11.43DD76 pKa = 3.78YY77 pKa = 10.75MVLDD81 pKa = 4.23GKK83 pKa = 10.68RR84 pKa = 11.84YY85 pKa = 10.31NVMGYY90 pKa = 9.25PKK92 pKa = 10.1SDD94 pKa = 2.97GVGVVIIPLEE104 pKa = 3.9ISEE107 pKa = 4.26

Molecular weight: 11.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z5D4|A0A6M9Z5D4_9CAUD Lysozyme OS=Acinetobacter phage vB_AbaP_Alexa OX=2736223 GN=Alexa_015 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.38RR3 pKa = 11.84KK4 pKa = 8.64QFKK7 pKa = 10.68RR8 pKa = 11.84NVQAQRR14 pKa = 11.84QRR16 pKa = 11.84RR17 pKa = 11.84SVTYY21 pKa = 10.39MNDD24 pKa = 2.99RR25 pKa = 11.84KK26 pKa = 10.97YY27 pKa = 11.26KK28 pKa = 10.04FIEE31 pKa = 4.07NLKK34 pKa = 8.96HH35 pKa = 6.38AGVGGLVGAFLVAVMFKK52 pKa = 11.11GLGVWW57 pKa = 3.43

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12647

28

760

218.1

24.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.844 ± 0.398

0.988 ± 0.128

6.128 ± 0.212

5.938 ± 0.35

3.353 ± 0.163

8.231 ± 0.325

1.803 ± 0.203

5.867 ± 0.188

6.057 ± 0.341

7.781 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.214

4.673 ± 0.375

4.317 ± 0.291

3.954 ± 0.236

5.527 ± 0.303

5.219 ± 0.278

6.594 ± 0.414

6.697 ± 0.269

1.629 ± 0.145

2.886 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski