Amino acid dipepetide frequency for Pseudomonas phage LUZ7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.833AlaAla: 10.833 ± 1.35
0.714AlaCys: 0.714 ± 0.231
5.333AlaAsp: 5.333 ± 0.562
6.844AlaGlu: 6.844 ± 0.642
3.233AlaPhe: 3.233 ± 0.339
6.256AlaGly: 6.256 ± 0.724
1.722AlaHis: 1.722 ± 0.266
5.081AlaIle: 5.081 ± 0.455
6.508AlaLys: 6.508 ± 0.682
7.978AlaLeu: 7.978 ± 0.689
2.561AlaMet: 2.561 ± 0.276
3.611AlaAsn: 3.611 ± 0.384
2.729AlaPro: 2.729 ± 0.444
4.577AlaGln: 4.577 ± 0.792
4.619AlaArg: 4.619 ± 0.467
5.123AlaSer: 5.123 ± 0.65
5.207AlaThr: 5.207 ± 0.591
5.836AlaVal: 5.836 ± 0.608
1.176AlaTrp: 1.176 ± 0.261
2.519AlaTyr: 2.519 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.462CysAla: 0.462 ± 0.137
0.126CysCys: 0.126 ± 0.075
0.336CysAsp: 0.336 ± 0.128
0.84CysGlu: 0.84 ± 0.215
0.084CysPhe: 0.084 ± 0.061
0.966CysGly: 0.966 ± 0.249
0.21CysHis: 0.21 ± 0.097
0.42CysIle: 0.42 ± 0.138
0.798CysLys: 0.798 ± 0.259
0.798CysLeu: 0.798 ± 0.233
0.126CysMet: 0.126 ± 0.076
0.63CysAsn: 0.63 ± 0.187
0.378CysPro: 0.378 ± 0.14
0.294CysGln: 0.294 ± 0.113
0.63CysArg: 0.63 ± 0.184
0.588CysSer: 0.588 ± 0.178
0.504CysThr: 0.504 ± 0.173
0.882CysVal: 0.882 ± 0.243
0.126CysTrp: 0.126 ± 0.073
0.294CysTyr: 0.294 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
6.088AspAla: 6.088 ± 0.851
0.546AspCys: 0.546 ± 0.151
2.855AspAsp: 2.855 ± 0.299
4.115AspGlu: 4.115 ± 0.415
2.393AspPhe: 2.393 ± 0.293
3.695AspGly: 3.695 ± 0.445
1.134AspHis: 1.134 ± 0.25
2.981AspIle: 2.981 ± 0.275
3.065AspLys: 3.065 ± 0.308
6.214AspLeu: 6.214 ± 0.45
1.512AspMet: 1.512 ± 0.227
2.183AspAsn: 2.183 ± 0.241
2.813AspPro: 2.813 ± 0.359
2.267AspGln: 2.267 ± 0.321
2.519AspArg: 2.519 ± 0.308
3.653AspSer: 3.653 ± 0.46
3.065AspThr: 3.065 ± 0.424
2.897AspVal: 2.897 ± 0.404
1.05AspTrp: 1.05 ± 0.224
2.141AspTyr: 2.141 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
7.768GluAla: 7.768 ± 0.994
0.588GluCys: 0.588 ± 0.147
3.947GluAsp: 3.947 ± 0.437
6.004GluGlu: 6.004 ± 0.743
2.267GluPhe: 2.267 ± 0.368
4.829GluGly: 4.829 ± 0.538
1.26GluHis: 1.26 ± 0.195
3.443GluIle: 3.443 ± 0.326
3.149GluLys: 3.149 ± 0.357
6.592GluLeu: 6.592 ± 0.46
2.435GluMet: 2.435 ± 0.321
2.267GluAsn: 2.267 ± 0.309
1.68GluPro: 1.68 ± 0.253
3.569GluGln: 3.569 ± 0.635
3.695GluArg: 3.695 ± 0.37
4.787GluSer: 4.787 ± 0.445
3.233GluThr: 3.233 ± 0.332
5.375GluVal: 5.375 ± 0.492
0.924GluTrp: 0.924 ± 0.198
2.141GluTyr: 2.141 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
2.225PheAla: 2.225 ± 0.34
0.084PheCys: 0.084 ± 0.063
2.855PheAsp: 2.855 ± 0.325
2.015PheGlu: 2.015 ± 0.275
1.764PhePhe: 1.764 ± 0.253
2.099PheGly: 2.099 ± 0.293
1.008PheHis: 1.008 ± 0.242
2.141PheIle: 2.141 ± 0.335
2.687PheLys: 2.687 ± 0.311
2.687PheLeu: 2.687 ± 0.33
1.176PheMet: 1.176 ± 0.237
2.057PheAsn: 2.057 ± 0.276
1.176PhePro: 1.176 ± 0.28
1.554PheGln: 1.554 ± 0.251
2.309PheArg: 2.309 ± 0.302
2.225PheSer: 2.225 ± 0.396
2.015PheThr: 2.015 ± 0.342
2.603PheVal: 2.603 ± 0.336
0.588PheTrp: 0.588 ± 0.184
0.84PheTyr: 0.84 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
5.752GlyAla: 5.752 ± 0.542
0.462GlyCys: 0.462 ± 0.143
3.653GlyAsp: 3.653 ± 0.48
3.653GlyGlu: 3.653 ± 0.315
3.653GlyPhe: 3.653 ± 0.344
4.367GlyGly: 4.367 ± 0.641
1.008GlyHis: 1.008 ± 0.191
3.359GlyIle: 3.359 ± 0.373
4.241GlyLys: 4.241 ± 0.442
6.508GlyLeu: 6.508 ± 0.492
2.435GlyMet: 2.435 ± 0.292
3.107GlyAsn: 3.107 ± 0.344
1.806GlyPro: 1.806 ± 0.275
2.813GlyGln: 2.813 ± 0.337
2.645GlyArg: 2.645 ± 0.353
4.493GlySer: 4.493 ± 0.396
3.737GlyThr: 3.737 ± 0.477
5.417GlyVal: 5.417 ± 0.445
0.882GlyTrp: 0.882 ± 0.211
2.813GlyTyr: 2.813 ± 0.339
0.0GlyXaa: 0.0 ± 0.0
His
1.512HisAla: 1.512 ± 0.242
0.084HisCys: 0.084 ± 0.057
1.26HisAsp: 1.26 ± 0.232
1.218HisGlu: 1.218 ± 0.195
1.134HisPhe: 1.134 ± 0.224
0.966HisGly: 0.966 ± 0.226
0.42HisHis: 0.42 ± 0.157
1.092HisIle: 1.092 ± 0.275
1.05HisLys: 1.05 ± 0.237
2.141HisLeu: 2.141 ± 0.347
0.882HisMet: 0.882 ± 0.207
0.84HisAsn: 0.84 ± 0.168
1.008HisPro: 1.008 ± 0.216
0.672HisGln: 0.672 ± 0.154
1.008HisArg: 1.008 ± 0.22
0.966HisSer: 0.966 ± 0.28
1.302HisThr: 1.302 ± 0.196
1.092HisVal: 1.092 ± 0.268
0.336HisTrp: 0.336 ± 0.136
1.176HisTyr: 1.176 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
4.115IleAla: 4.115 ± 0.393
0.672IleCys: 0.672 ± 0.185
3.107IleAsp: 3.107 ± 0.473
3.107IleGlu: 3.107 ± 0.353
1.386IlePhe: 1.386 ± 0.226
2.981IleGly: 2.981 ± 0.313
1.092IleHis: 1.092 ± 0.21
1.889IleIle: 1.889 ± 0.331
2.939IleLys: 2.939 ± 0.384
4.367IleLeu: 4.367 ± 0.424
1.302IleMet: 1.302 ± 0.221
2.687IleAsn: 2.687 ± 0.323
2.687IlePro: 2.687 ± 0.374
2.225IleGln: 2.225 ± 0.364
2.855IleArg: 2.855 ± 0.314
2.939IleSer: 2.939 ± 0.389
2.897IleThr: 2.897 ± 0.279
3.233IleVal: 3.233 ± 0.37
0.546IleTrp: 0.546 ± 0.169
1.722IleTyr: 1.722 ± 0.328
0.0IleXaa: 0.0 ± 0.0
Lys
6.76LysAla: 6.76 ± 0.611
0.504LysCys: 0.504 ± 0.153
3.317LysAsp: 3.317 ± 0.371
4.955LysGlu: 4.955 ± 0.623
1.847LysPhe: 1.847 ± 0.282
3.737LysGly: 3.737 ± 0.459
1.176LysHis: 1.176 ± 0.287
2.141LysIle: 2.141 ± 0.302
3.863LysLys: 3.863 ± 0.558
5.668LysLeu: 5.668 ± 0.616
1.554LysMet: 1.554 ± 0.246
2.561LysAsn: 2.561 ± 0.337
2.393LysPro: 2.393 ± 0.339
3.065LysGln: 3.065 ± 0.372
2.897LysArg: 2.897 ± 0.298
3.191LysSer: 3.191 ± 0.414
3.401LysThr: 3.401 ± 0.32
4.703LysVal: 4.703 ± 0.403
0.714LysTrp: 0.714 ± 0.161
2.351LysTyr: 2.351 ± 0.355
0.0LysXaa: 0.0 ± 0.0
Leu
7.054LeuAla: 7.054 ± 0.6
1.344LeuCys: 1.344 ± 0.352
5.459LeuAsp: 5.459 ± 0.571
6.172LeuGlu: 6.172 ± 0.485
3.065LeuPhe: 3.065 ± 0.337
5.584LeuGly: 5.584 ± 0.419
1.722LeuHis: 1.722 ± 0.194
4.325LeuIle: 4.325 ± 0.437
6.172LeuLys: 6.172 ± 0.591
8.776LeuLeu: 8.776 ± 0.831
2.981LeuMet: 2.981 ± 0.383
4.031LeuAsn: 4.031 ± 0.401
4.955LeuPro: 4.955 ± 0.464
3.275LeuGln: 3.275 ± 0.302
5.626LeuArg: 5.626 ± 0.617
6.256LeuSer: 6.256 ± 0.525
4.955LeuThr: 4.955 ± 0.511
6.214LeuVal: 6.214 ± 0.56
1.05LeuTrp: 1.05 ± 0.244
2.981LeuTyr: 2.981 ± 0.431
0.0LeuXaa: 0.0 ± 0.0
Met
3.233MetAla: 3.233 ± 0.394
0.462MetCys: 0.462 ± 0.152
1.722MetAsp: 1.722 ± 0.245
2.351MetGlu: 2.351 ± 0.254
0.798MetPhe: 0.798 ± 0.162
1.889MetGly: 1.889 ± 0.344
0.798MetHis: 0.798 ± 0.206
1.344MetIle: 1.344 ± 0.22
1.512MetLys: 1.512 ± 0.266
2.981MetLeu: 2.981 ± 0.379
0.798MetMet: 0.798 ± 0.188
1.512MetAsn: 1.512 ± 0.302
1.596MetPro: 1.596 ± 0.285
1.428MetGln: 1.428 ± 0.266
1.47MetArg: 1.47 ± 0.248
1.889MetSer: 1.889 ± 0.26
2.225MetThr: 2.225 ± 0.356
1.931MetVal: 1.931 ± 0.238
0.294MetTrp: 0.294 ± 0.115
1.176MetTyr: 1.176 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
4.367AsnAla: 4.367 ± 0.439
0.378AsnCys: 0.378 ± 0.124
1.806AsnAsp: 1.806 ± 0.389
2.603AsnGlu: 2.603 ± 0.276
1.47AsnPhe: 1.47 ± 0.265
3.191AsnGly: 3.191 ± 0.416
1.008AsnHis: 1.008 ± 0.216
2.351AsnIle: 2.351 ± 0.351
2.729AsnLys: 2.729 ± 0.342
4.661AsnLeu: 4.661 ± 0.43
1.302AsnMet: 1.302 ± 0.258
1.68AsnAsn: 1.68 ± 0.235
2.435AsnPro: 2.435 ± 0.359
2.309AsnGln: 2.309 ± 0.362
2.645AsnArg: 2.645 ± 0.35
2.477AsnSer: 2.477 ± 0.301
3.023AsnThr: 3.023 ± 0.35
2.771AsnVal: 2.771 ± 0.28
0.756AsnTrp: 0.756 ± 0.227
1.428AsnTyr: 1.428 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
3.569ProAla: 3.569 ± 0.386
0.168ProCys: 0.168 ± 0.077
2.435ProAsp: 2.435 ± 0.349
3.611ProGlu: 3.611 ± 0.432
1.554ProPhe: 1.554 ± 0.225
3.275ProGly: 3.275 ± 0.542
0.714ProHis: 0.714 ± 0.203
1.638ProIle: 1.638 ± 0.255
1.68ProLys: 1.68 ± 0.284
2.897ProLeu: 2.897 ± 0.327
1.344ProMet: 1.344 ± 0.255
1.973ProAsn: 1.973 ± 0.315
1.092ProPro: 1.092 ± 0.263
1.596ProGln: 1.596 ± 0.223
1.47ProArg: 1.47 ± 0.234
2.183ProSer: 2.183 ± 0.288
2.771ProThr: 2.771 ± 0.426
2.855ProVal: 2.855 ± 0.352
0.714ProTrp: 0.714 ± 0.225
1.218ProTyr: 1.218 ± 0.246
0.0ProXaa: 0.0 ± 0.0
Gln
4.871GlnAla: 4.871 ± 0.641
0.294GlnCys: 0.294 ± 0.096
2.729GlnAsp: 2.729 ± 0.35
3.527GlnGlu: 3.527 ± 0.509
1.008GlnPhe: 1.008 ± 0.146
2.897GlnGly: 2.897 ± 0.362
0.756GlnHis: 0.756 ± 0.187
2.225GlnIle: 2.225 ± 0.418
2.981GlnLys: 2.981 ± 0.344
4.157GlnLeu: 4.157 ± 0.382
1.428GlnMet: 1.428 ± 0.253
1.638GlnAsn: 1.638 ± 0.25
1.428GlnPro: 1.428 ± 0.239
2.099GlnGln: 2.099 ± 0.332
2.351GlnArg: 2.351 ± 0.348
1.889GlnSer: 1.889 ± 0.269
2.267GlnThr: 2.267 ± 0.328
3.527GlnVal: 3.527 ± 0.478
0.588GlnTrp: 0.588 ± 0.166
1.26GlnTyr: 1.26 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
3.359ArgAla: 3.359 ± 0.401
0.504ArgCys: 0.504 ± 0.173
3.275ArgAsp: 3.275 ± 0.459
4.157ArgGlu: 4.157 ± 0.625
1.889ArgPhe: 1.889 ± 0.291
3.149ArgGly: 3.149 ± 0.43
0.924ArgHis: 0.924 ± 0.219
2.729ArgIle: 2.729 ± 0.292
3.737ArgLys: 3.737 ± 0.359
5.207ArgLeu: 5.207 ± 0.475
1.847ArgMet: 1.847 ± 0.27
2.855ArgAsn: 2.855 ± 0.358
1.344ArgPro: 1.344 ± 0.319
2.141ArgGln: 2.141 ± 0.366
3.023ArgArg: 3.023 ± 0.342
3.527ArgSer: 3.527 ± 0.346
3.023ArgThr: 3.023 ± 0.375
4.115ArgVal: 4.115 ± 0.367
0.588ArgTrp: 0.588 ± 0.189
1.847ArgTyr: 1.847 ± 0.267
0.0ArgXaa: 0.0 ± 0.0
Ser
4.871SerAla: 4.871 ± 0.481
0.504SerCys: 0.504 ± 0.175
3.107SerAsp: 3.107 ± 0.318
3.905SerGlu: 3.905 ± 0.5
2.351SerPhe: 2.351 ± 0.343
4.787SerGly: 4.787 ± 0.547
1.386SerHis: 1.386 ± 0.254
3.401SerIle: 3.401 ± 0.437
3.695SerLys: 3.695 ± 0.439
5.417SerLeu: 5.417 ± 0.441
1.764SerMet: 1.764 ± 0.247
3.443SerAsn: 3.443 ± 0.386
1.847SerPro: 1.847 ± 0.242
2.351SerGln: 2.351 ± 0.29
3.275SerArg: 3.275 ± 0.41
3.653SerSer: 3.653 ± 0.361
3.443SerThr: 3.443 ± 0.33
3.905SerVal: 3.905 ± 0.423
0.924SerTrp: 0.924 ± 0.182
1.931SerTyr: 1.931 ± 0.21
0.0SerXaa: 0.0 ± 0.0
Thr
5.668ThrAla: 5.668 ± 0.519
0.672ThrCys: 0.672 ± 0.164
3.191ThrAsp: 3.191 ± 0.379
3.443ThrGlu: 3.443 ± 0.365
2.057ThrPhe: 2.057 ± 0.253
4.325ThrGly: 4.325 ± 0.467
1.47ThrHis: 1.47 ± 0.338
3.275ThrIle: 3.275 ± 0.485
3.233ThrLys: 3.233 ± 0.392
4.283ThrLeu: 4.283 ± 0.449
1.764ThrMet: 1.764 ± 0.28
2.981ThrAsn: 2.981 ± 0.435
2.855ThrPro: 2.855 ± 0.315
2.225ThrGln: 2.225 ± 0.357
2.687ThrArg: 2.687 ± 0.262
3.275ThrSer: 3.275 ± 0.498
2.771ThrThr: 2.771 ± 0.289
3.947ThrVal: 3.947 ± 0.413
0.924ThrTrp: 0.924 ± 0.244
1.806ThrTyr: 1.806 ± 0.315
0.0ThrXaa: 0.0 ± 0.0
Val
6.55ValAla: 6.55 ± 0.654
0.798ValCys: 0.798 ± 0.232
3.989ValAsp: 3.989 ± 0.457
4.493ValGlu: 4.493 ± 0.382
2.015ValPhe: 2.015 ± 0.395
4.619ValGly: 4.619 ± 0.46
1.302ValHis: 1.302 ± 0.212
3.233ValIle: 3.233 ± 0.374
4.115ValLys: 4.115 ± 0.396
5.836ValLeu: 5.836 ± 0.417
2.351ValMet: 2.351 ± 0.302
3.191ValAsn: 3.191 ± 0.347
2.687ValPro: 2.687 ± 0.474
3.443ValGln: 3.443 ± 0.317
3.947ValArg: 3.947 ± 0.411
4.031ValSer: 4.031 ± 0.451
4.367ValThr: 4.367 ± 0.333
4.325ValVal: 4.325 ± 0.534
1.008ValTrp: 1.008 ± 0.173
2.519ValTyr: 2.519 ± 0.49
0.0ValXaa: 0.0 ± 0.0
Trp
1.176TrpAla: 1.176 ± 0.236
0.168TrpCys: 0.168 ± 0.078
1.008TrpAsp: 1.008 ± 0.238
0.924TrpGlu: 0.924 ± 0.182
0.546TrpPhe: 0.546 ± 0.167
1.008TrpGly: 1.008 ± 0.209
0.126TrpHis: 0.126 ± 0.065
0.588TrpIle: 0.588 ± 0.167
0.84TrpLys: 0.84 ± 0.177
0.966TrpLeu: 0.966 ± 0.241
0.63TrpMet: 0.63 ± 0.175
0.63TrpAsn: 0.63 ± 0.169
0.504TrpPro: 0.504 ± 0.168
0.672TrpGln: 0.672 ± 0.171
0.84TrpArg: 0.84 ± 0.176
0.882TrpSer: 0.882 ± 0.192
0.63TrpThr: 0.63 ± 0.185
1.26TrpVal: 1.26 ± 0.275
0.21TrpTrp: 0.21 ± 0.109
0.42TrpTyr: 0.42 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.225TyrAla: 2.225 ± 0.326
0.42TyrCys: 0.42 ± 0.145
1.806TyrAsp: 1.806 ± 0.232
2.015TyrGlu: 2.015 ± 0.287
1.596TyrPhe: 1.596 ± 0.27
2.225TyrGly: 2.225 ± 0.448
0.924TyrHis: 0.924 ± 0.233
1.092TyrIle: 1.092 ± 0.236
1.806TyrLys: 1.806 ± 0.326
3.863TyrLeu: 3.863 ± 0.462
1.176TyrMet: 1.176 ± 0.238
1.512TyrAsn: 1.512 ± 0.237
1.344TyrPro: 1.344 ± 0.313
1.344TyrGln: 1.344 ± 0.261
2.729TyrArg: 2.729 ± 0.36
1.931TyrSer: 1.931 ± 0.295
2.015TyrThr: 2.015 ± 0.22
1.931TyrVal: 1.931 ± 0.402
0.63TyrTrp: 0.63 ± 0.174
1.344TyrTyr: 1.344 ± 0.266
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 115 proteins (23817 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski