Tunisvirus fontaine2

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Tunisvirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9SEF3|V9SEF3_9VIRU Uncharacterized protein OS=Tunisvirus fontaine2 OX=1421067 GN=TNS_ORF377 PE=4 SV=1
MM1 pKa = 7.43SFQVISSTSLNIIPGTFYY19 pKa = 11.47NLGEE23 pKa = 4.25LMEE26 pKa = 4.46VMNQQTVGDD35 pKa = 4.02DD36 pKa = 3.69SEE38 pKa = 4.45MPEE41 pKa = 4.43LVDD44 pKa = 4.35VPNEE48 pKa = 4.08DD49 pKa = 4.71GYY51 pKa = 11.87VADD54 pKa = 5.43DD55 pKa = 4.85GEE57 pKa = 4.28FADD60 pKa = 5.83DD61 pKa = 4.27EE62 pKa = 4.43EE63 pKa = 5.76ALGGPHH69 pKa = 7.16LEE71 pKa = 4.1ALRR74 pKa = 11.84TQIFQLFGHH83 pKa = 6.37DD84 pKa = 2.99EE85 pKa = 4.42CFNNLCPCGEE95 pKa = 4.0CDD97 pKa = 3.68EE98 pKa = 5.22YY99 pKa = 11.27KK100 pKa = 10.69GQPLVYY106 pKa = 9.76HH107 pKa = 6.69KK108 pKa = 11.04AKK110 pKa = 9.72FDD112 pKa = 3.52HH113 pKa = 6.94SITVEE118 pKa = 4.04DD119 pKa = 4.29FSEE122 pKa = 4.41YY123 pKa = 11.11SLFEE127 pKa = 3.88RR128 pKa = 11.84TDD130 pKa = 3.37YY131 pKa = 11.08FF132 pKa = 4.66

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9SED1|V9SED1_9VIRU Uncharacterized protein OS=Tunisvirus fontaine2 OX=1421067 GN=TNS_ORF372 PE=4 SV=1
MM1 pKa = 6.72QHH3 pKa = 6.59LVNPEE8 pKa = 3.65TGRR11 pKa = 11.84NILRR15 pKa = 11.84NGPTHH20 pKa = 7.3LSLLRR25 pKa = 11.84RR26 pKa = 11.84GIIPKK31 pKa = 9.58EE32 pKa = 4.01KK33 pKa = 9.91PLEE36 pKa = 4.06RR37 pKa = 11.84TKK39 pKa = 10.97KK40 pKa = 8.71GTLPGASNVSKK51 pKa = 10.86YY52 pKa = 10.44KK53 pKa = 10.39KK54 pKa = 9.42EE55 pKa = 3.93HH56 pKa = 6.27LKK58 pKa = 11.1SSDD61 pKa = 3.17FCGTVPGSFPVNSKK75 pKa = 9.71GRR77 pKa = 11.84ARR79 pKa = 11.84AALAYY84 pKa = 9.86ARR86 pKa = 11.84NDD88 pKa = 3.12MHH90 pKa = 6.95PEE92 pKa = 3.71RR93 pKa = 11.84VRR95 pKa = 11.84ACARR99 pKa = 11.84AKK101 pKa = 10.43AKK103 pKa = 10.65RR104 pKa = 11.84EE105 pKa = 3.75GWFF108 pKa = 3.3

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

484

0

484

112932

46

1651

233.3

26.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.026 ± 0.105

2.469 ± 0.075

4.929 ± 0.076

8.544 ± 0.141

5.573 ± 0.093

6.091 ± 0.122

1.886 ± 0.062

5.095 ± 0.075

8.816 ± 0.168

8.903 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.048

4.024 ± 0.075

3.858 ± 0.08

3.295 ± 0.079

5.502 ± 0.091

7.361 ± 0.092

5.139 ± 0.125

6.58 ± 0.086

1.495 ± 0.043

3.306 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski