Rhodopirellula sp. MGV

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula; unclassified Rhodopirellula

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255QV90|A0A255QV90_9BACT Phosphate import ATP-binding protein PstB OS=Rhodopirellula sp. MGV OX=2023130 GN=pstB PE=3 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 9.77LSFEE8 pKa = 3.97SMEE11 pKa = 3.98ARR13 pKa = 11.84RR14 pKa = 11.84LLAADD19 pKa = 3.2VAFNHH24 pKa = 6.29EE25 pKa = 4.35EE26 pKa = 3.93PTFEE30 pKa = 4.12VASDD34 pKa = 3.42ISAKK38 pKa = 10.57AFADD42 pKa = 3.09IGNLDD47 pKa = 3.42GTAIISSSLGWFNSYY62 pKa = 11.02DD63 pKa = 3.65RR64 pKa = 11.84IGFSVEE70 pKa = 3.54RR71 pKa = 11.84EE72 pKa = 3.6ADD74 pKa = 3.18VSIRR78 pKa = 11.84LNSFWTDD85 pKa = 2.26VDD87 pKa = 5.26LYY89 pKa = 10.23LTDD92 pKa = 3.06SRR94 pKa = 11.84GYY96 pKa = 10.53VIDD99 pKa = 4.43YY100 pKa = 9.57SVNWGTTAEE109 pKa = 4.58SIDD112 pKa = 3.25ITVNPGNYY120 pKa = 9.14YY121 pKa = 9.49VWSLATSWYY130 pKa = 5.03TTGYY134 pKa = 10.58RR135 pKa = 11.84MEE137 pKa = 4.26LTADD141 pKa = 3.56QVPEE145 pKa = 4.19PLPEE149 pKa = 4.29PEE151 pKa = 4.3PTPDD155 pKa = 3.37AGVPADD161 pKa = 4.3GVSPLPDD168 pKa = 2.84VAYY171 pKa = 10.59FGGSNEE177 pKa = 3.83WGLNAVGAPEE187 pKa = 3.93AWAAGYY193 pKa = 7.83TGQGVTVAVIDD204 pKa = 3.7TGVDD208 pKa = 3.35LDD210 pKa = 5.13HH211 pKa = 7.73PDD213 pKa = 3.85LYY215 pKa = 11.7SNIYY219 pKa = 9.37VNPGEE224 pKa = 4.11IAGNGIDD231 pKa = 5.14DD232 pKa = 5.04DD233 pKa = 5.1GNGYY237 pKa = 10.18VDD239 pKa = 4.78DD240 pKa = 3.73VHH242 pKa = 7.97GFDD245 pKa = 5.68FADD248 pKa = 5.07FDD250 pKa = 4.93ADD252 pKa = 3.79PNDD255 pKa = 3.57VHH257 pKa = 7.37GHH259 pKa = 4.6GTHH262 pKa = 5.72VAGTIAALDD271 pKa = 4.05NGWGATGVAPEE282 pKa = 4.03ATILPVKK289 pKa = 10.63VLGDD293 pKa = 3.67NGSGSSTSVAAGIRR307 pKa = 11.84YY308 pKa = 8.6AADD311 pKa = 3.07MGADD315 pKa = 4.15IINLSLGGGYY325 pKa = 10.3SSDD328 pKa = 3.2IEE330 pKa = 4.32SAINYY335 pKa = 8.38ARR337 pKa = 11.84TLGSLVIAAAGNEE350 pKa = 4.25YY351 pKa = 10.95ASMPGFPARR360 pKa = 11.84FSASYY365 pKa = 11.42DD366 pKa = 3.36NVLSVGAYY374 pKa = 10.28DD375 pKa = 3.49SGSNIASFSNDD386 pKa = 2.88VGGSGAVQVDD396 pKa = 3.78APGVGIFSTYY406 pKa = 10.22VGGGFGTMSGTSMATPHH423 pKa = 5.56VVGVAALTLSANPSLSPAALRR444 pKa = 11.84DD445 pKa = 3.75LLVSGVNGHH454 pKa = 7.11ANGSDD459 pKa = 3.5GLGKK463 pKa = 10.51ASTIYY468 pKa = 10.52SVAYY472 pKa = 9.79AAAGVSGSSTSGSGSTSTSGGSGSVGAASVGDD504 pKa = 3.87AGGSTLAGLSDD515 pKa = 4.87SIEE518 pKa = 4.49DD519 pKa = 3.9DD520 pKa = 3.11ATNADD525 pKa = 3.97RR526 pKa = 11.84VFEE529 pKa = 4.3SDD531 pKa = 3.6VQFDD535 pKa = 3.59SSPRR539 pKa = 11.84LARR542 pKa = 11.84SNRR545 pKa = 11.84PSVAPQPEE553 pKa = 4.28DD554 pKa = 3.1TDD556 pKa = 3.97AALSDD561 pKa = 3.83FFGDD565 pKa = 3.38SDD567 pKa = 4.03SDD569 pKa = 3.98NEE571 pKa = 4.22FGSDD575 pKa = 3.37RR576 pKa = 11.84EE577 pKa = 4.02VDD579 pKa = 3.57LGLLVLAA586 pKa = 5.61

Molecular weight: 59.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255R7Q9|A0A255R7Q9_9BACT Uncharacterized protein OS=Rhodopirellula sp. MGV OX=2023130 GN=CGZ80_07480 PE=4 SV=1
MM1 pKa = 7.17TLHH4 pKa = 7.1RR5 pKa = 11.84VPIPRR10 pKa = 11.84RR11 pKa = 11.84NTVAKK16 pKa = 10.43KK17 pKa = 10.3KK18 pKa = 8.91ATKK21 pKa = 10.26KK22 pKa = 10.4KK23 pKa = 8.66ATKK26 pKa = 10.15KK27 pKa = 10.11KK28 pKa = 9.49AAKK31 pKa = 9.89KK32 pKa = 10.03KK33 pKa = 8.36ATKK36 pKa = 10.13KK37 pKa = 9.97VATKK41 pKa = 10.49KK42 pKa = 9.02ATKK45 pKa = 10.21KK46 pKa = 10.44KK47 pKa = 8.63ATKK50 pKa = 10.25KK51 pKa = 10.39KK52 pKa = 8.55ATKK55 pKa = 10.25KK56 pKa = 10.39KK57 pKa = 8.55ATKK60 pKa = 10.25KK61 pKa = 10.39KK62 pKa = 8.55ATKK65 pKa = 10.25KK66 pKa = 10.39KK67 pKa = 8.55ATKK70 pKa = 10.25KK71 pKa = 10.39KK72 pKa = 8.66ATKK75 pKa = 10.09KK76 pKa = 9.83KK77 pKa = 9.18AAKK80 pKa = 9.59KK81 pKa = 10.04RR82 pKa = 11.84RR83 pKa = 3.58

Molecular weight: 9.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5273

0

5273

2047646

30

8214

388.3

42.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.497 ± 0.032

1.142 ± 0.016

6.492 ± 0.035

6.133 ± 0.038

3.746 ± 0.023

7.484 ± 0.041

2.117 ± 0.021

5.288 ± 0.021

3.495 ± 0.028

9.593 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.017

3.36 ± 0.024

4.95 ± 0.027

4.239 ± 0.023

6.546 ± 0.04

7.04 ± 0.031

5.682 ± 0.044

7.201 ± 0.031

1.409 ± 0.015

2.362 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski