Cycloclasticus sp. symbiont of Bathymodiolus heckerae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Cycloclasticus; unclassified Cycloclasticus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2010 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1QTT5|A0A1X1QTT5_9GAMM 50S ribosomal protein L11 OS=Cycloclasticus sp. symbiont of Bathymodiolus heckerae OX=1284675 GN=rplK PE=3 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84TFNKK6 pKa = 9.97KK7 pKa = 9.54PLALGVAAGMAMLVASTGAMAISAGTDD34 pKa = 3.25LNLSGKK40 pKa = 9.8ARR42 pKa = 11.84AGGMAGAAYY51 pKa = 8.83TMPQEE56 pKa = 3.77ASAAVFGNPATLSQFKK72 pKa = 9.99GINMNFGASWLSLSGVEE89 pKa = 4.84NVQTDD94 pKa = 3.66SLGGSNTSTSDD105 pKa = 2.9ADD107 pKa = 3.56NYY109 pKa = 10.25IIPDD113 pKa = 4.22FGLTIQVSPNLVIGTGLEE131 pKa = 3.73VDD133 pKa = 3.84AGLGADD139 pKa = 4.35YY140 pKa = 10.85RR141 pKa = 11.84DD142 pKa = 4.21DD143 pKa = 5.63PINLLPGAGDD153 pKa = 3.38FTLPLLVEE161 pKa = 4.61VISFNANLAAAYY173 pKa = 8.67QATDD177 pKa = 3.53QLSLGASVTVGFGLAQLGTAGATQGLTALNGLVNTGGNGAGAGIAVGPDD226 pKa = 2.63ILADD230 pKa = 4.23FGGTTSSVHH239 pKa = 7.17DD240 pKa = 3.64IGFGASLGAVYY251 pKa = 9.69EE252 pKa = 4.25VQEE255 pKa = 4.35GVLVSATVKK264 pKa = 10.98SPVEE268 pKa = 3.9YY269 pKa = 10.2NFKK272 pKa = 10.9NIIHH276 pKa = 7.35ADD278 pKa = 3.55PTAIAAAGGSIAADD292 pKa = 4.97GYY294 pKa = 11.63QDD296 pKa = 4.49LTLQQPAEE304 pKa = 4.43VILGIALNDD313 pKa = 3.55VIMPGLLIEE322 pKa = 5.95ADD324 pKa = 4.53AIWKK328 pKa = 8.91NWSDD332 pKa = 2.92AHH334 pKa = 7.77AYY336 pKa = 8.84EE337 pKa = 5.58DD338 pKa = 4.21AYY340 pKa = 10.57EE341 pKa = 4.29DD342 pKa = 3.37QWLLAIGAQYY352 pKa = 10.52EE353 pKa = 4.41AGDD356 pKa = 3.34FTYY359 pKa = 10.4RR360 pKa = 11.84VGYY363 pKa = 10.23SYY365 pKa = 11.75AEE367 pKa = 5.49DD368 pKa = 3.62ILSDD372 pKa = 3.87APNGTLSGLDD382 pKa = 3.49GLGSVPLYY390 pKa = 10.41DD391 pKa = 5.12DD392 pKa = 4.7PGANGALGALSVDD405 pKa = 4.06VITIVQNSLLPVIWNHH421 pKa = 6.72TITAGIGYY429 pKa = 9.31KK430 pKa = 8.9ITDD433 pKa = 3.78AVSVDD438 pKa = 3.49AYY440 pKa = 10.46AAYY443 pKa = 10.44AFGEE447 pKa = 4.28NEE449 pKa = 4.38KK450 pKa = 9.79DD451 pKa = 3.53TLSTLSTVADD461 pKa = 3.76TALGGSGLGGTLIEE475 pKa = 4.51NEE477 pKa = 3.87AKK479 pKa = 9.73IDD481 pKa = 3.71YY482 pKa = 10.2EE483 pKa = 4.32LMIGAGINIALPP495 pKa = 3.76

Molecular weight: 50.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1QUG2|A0A1X1QUG2_9GAMM Transposase OS=Cycloclasticus sp. symbiont of Bathymodiolus heckerae OX=1284675 GN=A6F70_09765 PE=4 SV=1
MM1 pKa = 7.61LGLGCPKK8 pKa = 10.21QAFAKK13 pKa = 10.05IATPRR18 pKa = 11.84GTRR21 pKa = 11.84GIRR24 pKa = 11.84LGQQLSLRR32 pKa = 11.84QVNRR36 pKa = 11.84VFVSLRR42 pKa = 11.84DD43 pKa = 3.4KK44 pKa = 11.11LGWVNRR50 pKa = 11.84GAHH53 pKa = 6.18DD54 pKa = 3.74APRR57 pKa = 11.84IHH59 pKa = 7.52DD60 pKa = 3.78LRR62 pKa = 11.84HH63 pKa = 4.95TFVVRR68 pKa = 11.84RR69 pKa = 11.84VMLWHH74 pKa = 5.82TQGINIDD81 pKa = 3.73QQMLALSTYY90 pKa = 9.46VGHH93 pKa = 7.57AMITNTYY100 pKa = 8.62WYY102 pKa = 8.6LTGVPQLMAVAAEE115 pKa = 4.1KK116 pKa = 10.89FEE118 pKa = 5.01AFTQIQEE125 pKa = 4.14ASHH128 pKa = 5.29VV129 pKa = 3.7

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2010

0

2010

614684

51

1614

305.8

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.393 ± 0.056

1.081 ± 0.02

5.707 ± 0.043

6.36 ± 0.061

4.049 ± 0.039

6.956 ± 0.061

2.271 ± 0.026

6.775 ± 0.04

6.041 ± 0.051

10.329 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.032

4.286 ± 0.037

3.831 ± 0.035

4.021 ± 0.043

4.501 ± 0.043

6.671 ± 0.038

5.301 ± 0.038

6.916 ± 0.047

1.127 ± 0.021

2.812 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski