Amino acid dipepetide frequency for bacterium HR36

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.331AlaAla: 11.331 ± 0.147	1.461AlaCys: 1.461 ± 0.047	5.045AlaAsp: 5.045 ± 0.08	7.099AlaGlu: 7.099 ± 0.112	3.164AlaPhe: 3.164 ± 0.079	7.79AlaGly: 7.79 ± 0.093	2.212AlaHis: 2.212 ± 0.058	4.807AlaIle: 4.807 ± 0.087	3.035AlaLys: 3.035 ± 0.063	11.476AlaLeu: 11.476 ± 0.137	1.918AlaMet: 1.918 ± 0.05	2.721AlaAsn: 2.721 ± 0.06	4.688AlaPro: 4.688 ± 0.08	4.963AlaGln: 4.963 ± 0.091	7.544AlaArg: 7.544 ± 0.097	5.445AlaSer: 5.445 ± 0.092	4.735AlaThr: 4.735 ± 0.085	7.404AlaVal: 7.404 ± 0.09	2.129AlaTrp: 2.129 ± 0.054	2.352AlaTyr: 2.352 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.101CysAla: 1.101 ± 0.039	0.265CysCys: 0.265 ± 0.02	0.689CysAsp: 0.689 ± 0.029	0.757CysGlu: 0.757 ± 0.03	0.462CysPhe: 0.462 ± 0.025	1.236CysGly: 1.236 ± 0.04	0.469CysHis: 0.469 ± 0.025	0.508CysIle: 0.508 ± 0.024	0.321CysLys: 0.321 ± 0.021	1.549CysLeu: 1.549 ± 0.046	0.184CysMet: 0.184 ± 0.013	0.282CysAsn: 0.282 ± 0.021	0.844CysPro: 0.844 ± 0.038	0.696CysGln: 0.696 ± 0.03	1.324CysArg: 1.324 ± 0.044	0.616CysSer: 0.616 ± 0.028	0.626CysThr: 0.626 ± 0.029	0.965CysVal: 0.965 ± 0.037	0.325CysTrp: 0.325 ± 0.019	0.454CysTyr: 0.454 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	4.226AspAla: 4.226 ± 0.068	0.551AspCys: 0.551 ± 0.029	1.994AspAsp: 1.994 ± 0.062	2.698AspGlu: 2.698 ± 0.063	1.917AspPhe: 1.917 ± 0.046	3.787AspGly: 3.787 ± 0.076	1.089AspHis: 1.089 ± 0.037	2.228AspIle: 2.228 ± 0.052	1.621AspLys: 1.621 ± 0.051	5.299AspLeu: 5.299 ± 0.084	0.789AspMet: 0.789 ± 0.029	1.212AspAsn: 1.212 ± 0.028	3.27AspPro: 3.27 ± 0.069	1.662AspGln: 1.662 ± 0.052	3.49AspArg: 3.49 ± 0.065	2.104AspSer: 2.104 ± 0.055	2.129AspThr: 2.129 ± 0.048	3.184AspVal: 3.184 ± 0.06	1.221AspTrp: 1.221 ± 0.042	1.534AspTyr: 1.534 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	6.345GluAla: 6.345 ± 0.11	0.633GluCys: 0.633 ± 0.03	2.544GluAsp: 2.544 ± 0.064	4.176GluGlu: 4.176 ± 0.095	2.027GluPhe: 2.027 ± 0.042	3.681GluGly: 3.681 ± 0.072	1.609GluHis: 1.609 ± 0.045	3.308GluIle: 3.308 ± 0.066	2.779GluLys: 2.779 ± 0.057	7.269GluLeu: 7.269 ± 0.105	1.361GluMet: 1.361 ± 0.045	1.592GluAsn: 1.592 ± 0.04	3.434GluPro: 3.434 ± 0.064	3.385GluGln: 3.385 ± 0.076	5.283GluArg: 5.283 ± 0.098	2.702GluSer: 2.702 ± 0.06	3.016GluThr: 3.016 ± 0.063	4.68GluVal: 4.68 ± 0.072	0.947GluTrp: 0.947 ± 0.036	1.584GluTyr: 1.584 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.64PheAla: 3.64 ± 0.067	0.592PheCys: 0.592 ± 0.029	1.971PheAsp: 1.971 ± 0.051	1.741PheGlu: 1.741 ± 0.043	1.382PhePhe: 1.382 ± 0.047	2.964PheGly: 2.964 ± 0.06	0.93PheHis: 0.93 ± 0.034	1.227PheIle: 1.227 ± 0.043	0.652PheLys: 0.652 ± 0.031	4.05PheLeu: 4.05 ± 0.074	0.403PheMet: 0.403 ± 0.023	0.802PheAsn: 0.802 ± 0.03	2.019PhePro: 2.019 ± 0.05	1.348PheGln: 1.348 ± 0.039	3.009PheArg: 3.009 ± 0.055	1.757PheSer: 1.757 ± 0.052	1.636PheThr: 1.636 ± 0.044	2.61PheVal: 2.61 ± 0.061	0.668PheTrp: 0.668 ± 0.027	0.935PheTyr: 0.935 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.639GlyAla: 5.639 ± 0.089	1.102GlyCys: 1.102 ± 0.04	3.368GlyAsp: 3.368 ± 0.066	4.586GlyGlu: 4.586 ± 0.073	2.679GlyPhe: 2.679 ± 0.06	5.51GlyGly: 5.51 ± 0.117	1.843GlyHis: 1.843 ± 0.051	3.686GlyIle: 3.686 ± 0.075	3.414GlyLys: 3.414 ± 0.077	7.817GlyLeu: 7.817 ± 0.101	1.586GlyMet: 1.586 ± 0.05	2.244GlyAsn: 2.244 ± 0.064	3.706GlyPro: 3.706 ± 0.072	4.305GlyGln: 4.305 ± 0.076	5.957GlyArg: 5.957 ± 0.086	3.98GlySer: 3.98 ± 0.068	3.917GlyThr: 3.917 ± 0.092	5.167GlyVal: 5.167 ± 0.074	1.575GlyTrp: 1.575 ± 0.045	2.273GlyTyr: 2.273 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	2.334HisAla: 2.334 ± 0.054	0.475HisCys: 0.475 ± 0.023	1.106HisAsp: 1.106 ± 0.034	1.285HisGlu: 1.285 ± 0.039	1.013HisPhe: 1.013 ± 0.04	2.03HisGly: 2.03 ± 0.053	0.859HisHis: 0.859 ± 0.034	1.109HisIle: 1.109 ± 0.04	0.605HisLys: 0.605 ± 0.027	3.055HisLeu: 3.055 ± 0.062	0.365HisMet: 0.365 ± 0.021	0.646HisAsn: 0.646 ± 0.023	1.874HisPro: 1.874 ± 0.048	1.148HisGln: 1.148 ± 0.04	2.079HisArg: 2.079 ± 0.061	1.14HisSer: 1.14 ± 0.035	1.126HisThr: 1.126 ± 0.038	1.715HisVal: 1.715 ± 0.045	0.604HisTrp: 0.604 ± 0.029	0.797HisTyr: 0.797 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	4.813IleAla: 4.813 ± 0.078	0.673IleCys: 0.673 ± 0.031	1.79IleAsp: 1.79 ± 0.045	2.789IleGlu: 2.789 ± 0.053	1.649IlePhe: 1.649 ± 0.047	3.556IleGly: 3.556 ± 0.077	1.12IleHis: 1.12 ± 0.035	1.952IleIle: 1.952 ± 0.054	1.132IleLys: 1.132 ± 0.04	4.796IleLeu: 4.796 ± 0.079	0.59IleMet: 0.59 ± 0.028	1.17IleAsn: 1.17 ± 0.038	2.601IlePro: 2.601 ± 0.056	1.686IleGln: 1.686 ± 0.044	3.862IleArg: 3.862 ± 0.065	2.418IleSer: 2.418 ± 0.055	2.525IleThr: 2.525 ± 0.063	3.718IleVal: 3.718 ± 0.067	0.734IleTrp: 0.734 ± 0.03	1.333IleTyr: 1.333 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	2.721LysAla: 2.721 ± 0.064	0.298LysCys: 0.298 ± 0.02	1.295LysAsp: 1.295 ± 0.039	1.895LysGlu: 1.895 ± 0.06	0.788LysPhe: 0.788 ± 0.033	1.839LysGly: 1.839 ± 0.051	0.714LysHis: 0.714 ± 0.027	1.538LysIle: 1.538 ± 0.047	1.234LysLys: 1.234 ± 0.044	3.377LysLeu: 3.377 ± 0.062	0.684LysMet: 0.684 ± 0.028	0.842LysAsn: 0.842 ± 0.033	2.75LysPro: 2.75 ± 0.074	1.394LysGln: 1.394 ± 0.039	2.41LysArg: 2.41 ± 0.059	1.442LysSer: 1.442 ± 0.047	1.663LysThr: 1.663 ± 0.044	2.088LysVal: 2.088 ± 0.052	0.468LysTrp: 0.468 ± 0.023	0.773LysTyr: 0.773 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	14.501LeuAla: 14.501 ± 0.144	1.544LeuCys: 1.544 ± 0.05	5.462LeuAsp: 5.462 ± 0.087	7.294LeuGlu: 7.294 ± 0.107	3.385LeuPhe: 3.385 ± 0.07	8.509LeuGly: 8.509 ± 0.12	2.734LeuHis: 2.734 ± 0.049	4.234LeuIle: 4.234 ± 0.07	2.698LeuLys: 2.698 ± 0.066	12.531LeuLeu: 12.531 ± 0.136	1.708LeuMet: 1.708 ± 0.052	2.26LeuAsn: 2.26 ± 0.062	7.14LeuPro: 7.14 ± 0.101	5.309LeuGln: 5.309 ± 0.089	10.232LeuArg: 10.232 ± 0.117	5.413LeuSer: 5.413 ± 0.081	5.448LeuThr: 5.448 ± 0.081	8.78LeuVal: 8.78 ± 0.108	1.718LeuTrp: 1.718 ± 0.054	2.582LeuTyr: 2.582 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	1.787MetAla: 1.787 ± 0.047	0.201MetCys: 0.201 ± 0.017	0.677MetAsp: 0.677 ± 0.031	0.957MetGlu: 0.957 ± 0.036	0.583MetPhe: 0.583 ± 0.026	1.23MetGly: 1.23 ± 0.039	0.418MetHis: 0.418 ± 0.023	0.737MetIle: 0.737 ± 0.031	0.556MetLys: 0.556 ± 0.025	2.104MetLeu: 2.104 ± 0.049	0.343MetMet: 0.343 ± 0.032	0.478MetAsn: 0.478 ± 0.022	1.296MetPro: 1.296 ± 0.035	0.872MetGln: 0.872 ± 0.034	1.549MetArg: 1.549 ± 0.042	1.098MetSer: 1.098 ± 0.037	0.968MetThr: 0.968 ± 0.036	1.293MetVal: 1.293 ± 0.043	0.224MetTrp: 0.224 ± 0.016	0.328MetTyr: 0.328 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.431AsnAla: 2.431 ± 0.062	0.346AsnCys: 0.346 ± 0.019	1.232AsnAsp: 1.232 ± 0.051	1.277AsnGlu: 1.277 ± 0.036	0.874AsnPhe: 0.874 ± 0.032	2.166AsnGly: 2.166 ± 0.072	0.597AsnHis: 0.597 ± 0.028	1.178AsnIle: 1.178 ± 0.036	0.727AsnLys: 0.727 ± 0.03	2.817AsnLeu: 2.817 ± 0.064	0.4AsnMet: 0.4 ± 0.022	0.844AsnAsn: 0.844 ± 0.043	2.028AsnPro: 2.028 ± 0.057	1.041AsnGln: 1.041 ± 0.04	2.074AsnArg: 2.074 ± 0.052	1.261AsnSer: 1.261 ± 0.039	1.267AsnThr: 1.267 ± 0.04	1.835AsnVal: 1.835 ± 0.05	0.577AsnTrp: 0.577 ± 0.027	0.729AsnTyr: 0.729 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.519ProAla: 6.519 ± 0.104	0.616ProCys: 0.616 ± 0.027	3.545ProAsp: 3.545 ± 0.065	4.506ProGlu: 4.506 ± 0.076	1.987ProPhe: 1.987 ± 0.049	5.24ProGly: 5.24 ± 0.085	1.627ProHis: 1.627 ± 0.045	2.667ProIle: 2.667 ± 0.058	1.922ProLys: 1.922 ± 0.046	5.574ProLeu: 5.574 ± 0.093	1.166ProMet: 1.166 ± 0.034	1.907ProAsn: 1.907 ± 0.055	4.578ProPro: 4.578 ± 0.1	2.89ProGln: 2.89 ± 0.056	4.22ProArg: 4.22 ± 0.075	3.448ProSer: 3.448 ± 0.076	3.131ProThr: 3.131 ± 0.062	4.223ProVal: 4.223 ± 0.079	0.941ProTrp: 0.941 ± 0.039	1.483ProTyr: 1.483 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	5.815GlnAla: 5.815 ± 0.105	0.587GlnCys: 0.587 ± 0.026	1.776GlnAsp: 1.776 ± 0.049	3.207GlnGlu: 3.207 ± 0.067	1.471GlnPhe: 1.471 ± 0.038	3.117GlnGly: 3.117 ± 0.065	1.302GlnHis: 1.302 ± 0.039	2.2GlnIle: 2.2 ± 0.052	1.386GlnLys: 1.386 ± 0.044	5.753GlnLeu: 5.753 ± 0.1	0.883GlnMet: 0.883 ± 0.038	1.069GlnAsn: 1.069 ± 0.041	3.138GlnPro: 3.138 ± 0.059	2.912GlnGln: 2.912 ± 0.081	4.59GlnArg: 4.59 ± 0.085	2.022GlnSer: 2.022 ± 0.05	2.29GlnThr: 2.29 ± 0.046	3.659GlnVal: 3.659 ± 0.076	0.811GlnTrp: 0.811 ± 0.037	1.011GlnTyr: 1.011 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	6.616ArgAla: 6.616 ± 0.091	1.194ArgCys: 1.194 ± 0.041	3.791ArgAsp: 3.791 ± 0.069	5.425ArgGlu: 5.425 ± 0.091	3.241ArgPhe: 3.241 ± 0.057	4.969ArgGly: 4.969 ± 0.073	2.192ArgHis: 2.192 ± 0.061	4.187ArgIle: 4.187 ± 0.066	2.557ArgLys: 2.557 ± 0.06	10.351ArgLeu: 10.351 ± 0.113	1.661ArgMet: 1.661 ± 0.042	1.943ArgAsn: 1.943 ± 0.05	4.611ArgPro: 4.611 ± 0.091	5.38ArgGln: 5.38 ± 0.096	7.356ArgArg: 7.356 ± 0.125	3.806ArgSer: 3.806 ± 0.073	3.462ArgThr: 3.462 ± 0.067	5.448ArgVal: 5.448 ± 0.082	1.927ArgTrp: 1.927 ± 0.051	2.41ArgTyr: 2.41 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	5.268SerAla: 5.268 ± 0.085	0.631SerCys: 0.631 ± 0.026	2.397SerAsp: 2.397 ± 0.062	2.929SerGlu: 2.929 ± 0.063	1.623SerPhe: 1.623 ± 0.044	4.478SerGly: 4.478 ± 0.077	1.147SerHis: 1.147 ± 0.04	1.752SerIle: 1.752 ± 0.048	1.331SerLys: 1.331 ± 0.042	5.428SerLeu: 5.428 ± 0.089	0.93SerMet: 0.93 ± 0.035	1.288SerAsn: 1.288 ± 0.043	3.741SerPro: 3.741 ± 0.066	2.284SerGln: 2.284 ± 0.05	3.905SerArg: 3.905 ± 0.079	2.929SerSer: 2.929 ± 0.064	2.221SerThr: 2.221 ± 0.055	3.633SerVal: 3.633 ± 0.066	1.11SerTrp: 1.11 ± 0.039	1.174SerTyr: 1.174 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	4.826ThrAla: 4.826 ± 0.082	0.62ThrCys: 0.62 ± 0.028	2.22ThrAsp: 2.22 ± 0.047	2.483ThrGlu: 2.483 ± 0.052	1.657ThrPhe: 1.657 ± 0.049	4.254ThrGly: 4.254 ± 0.07	1.175ThrHis: 1.175 ± 0.042	2.333ThrIle: 2.333 ± 0.059	1.211ThrLys: 1.211 ± 0.037	5.591ThrLeu: 5.591 ± 0.089	0.782ThrMet: 0.782 ± 0.034	1.282ThrAsn: 1.282 ± 0.047	3.368ThrPro: 3.368 ± 0.072	1.922ThrGln: 1.922 ± 0.043	3.069ThrArg: 3.069 ± 0.059	2.541ThrSer: 2.541 ± 0.056	2.209ThrThr: 2.209 ± 0.061	3.913ThrVal: 3.913 ± 0.081	0.963ThrTrp: 0.963 ± 0.042	1.187ThrTyr: 1.187 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	7.493ValAla: 7.493 ± 0.1	1.129ValCys: 1.129 ± 0.035	2.902ValAsp: 2.902 ± 0.059	4.65ValGlu: 4.65 ± 0.077	2.636ValPhe: 2.636 ± 0.061	5.039ValGly: 5.039 ± 0.086	1.866ValHis: 1.866 ± 0.044	3.449ValIle: 3.449 ± 0.07	1.874ValLys: 1.874 ± 0.046	8.654ValLeu: 8.654 ± 0.113	1.185ValMet: 1.185 ± 0.04	1.959ValAsn: 1.959 ± 0.057	4.432ValPro: 4.432 ± 0.073	3.173ValGln: 3.173 ± 0.062	6.284ValArg: 6.284 ± 0.077	3.893ValSer: 3.893 ± 0.08	3.36ValThr: 3.36 ± 0.068	5.884ValVal: 5.884 ± 0.103	1.389ValTrp: 1.389 ± 0.043	1.937ValTyr: 1.937 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	1.593TrpAla: 1.593 ± 0.05	0.289TrpCys: 0.289 ± 0.02	0.812TrpAsp: 0.812 ± 0.036	1.044TrpGlu: 1.044 ± 0.036	0.721TrpPhe: 0.721 ± 0.032	1.001TrpGly: 1.001 ± 0.037	0.687TrpHis: 0.687 ± 0.028	0.756TrpIle: 0.756 ± 0.03	0.567TrpLys: 0.567 ± 0.024	2.851TrpLeu: 2.851 ± 0.065	0.363TrpMet: 0.363 ± 0.019	0.489TrpAsn: 0.489 ± 0.025	1.175TrpPro: 1.175 ± 0.048	1.363TrpGln: 1.363 ± 0.044	1.794TrpArg: 1.794 ± 0.047	0.972TrpSer: 0.972 ± 0.034	0.791TrpThr: 0.791 ± 0.031	1.113TrpVal: 1.113 ± 0.039	0.535TrpTrp: 0.535 ± 0.029	0.53TrpTyr: 0.53 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.318TyrAla: 2.318 ± 0.048	0.468TyrCys: 0.468 ± 0.024	1.389TyrAsp: 1.389 ± 0.049	1.553TyrGlu: 1.553 ± 0.041	1.075TyrPhe: 1.075 ± 0.038	1.956TyrGly: 1.956 ± 0.06	0.774TyrHis: 0.774 ± 0.027	1.052TyrIle: 1.052 ± 0.037	0.593TyrLys: 0.593 ± 0.026	3.142TyrLeu: 3.142 ± 0.066	0.397TyrMet: 0.397 ± 0.024	0.671TyrAsn: 0.671 ± 0.032	1.5TyrPro: 1.5 ± 0.041	1.264TyrGln: 1.264 ± 0.035	2.496TyrArg: 2.496 ± 0.056	1.213TyrSer: 1.213 ± 0.04	1.086TyrThr: 1.086 ± 0.039	1.877TyrVal: 1.877 ± 0.05	0.581TyrTrp: 0.581 ± 0.032	1.027TyrTyr: 1.027 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2862 proteins (869442 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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