Rhodothermaceae bacterium RA

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes Order II. Incertae sedis; Rhodothermaceae; unclassified Rhodothermaceae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0DB91|A0A1V0DB91_9BACT ATP-binding protein OS=Rhodothermaceae bacterium RA OX=1779382 GN=AWN76_003395 PE=4 SV=1
MM1 pKa = 7.02TRR3 pKa = 11.84FVRR6 pKa = 11.84PLLPALLGAMLLLSACAEE24 pKa = 4.45GPVGPPGPPGNANVFSLTFLFTMDD48 pKa = 4.46DD49 pKa = 3.56ASISGPVASVGYY61 pKa = 8.26EE62 pKa = 4.01VPAITPSVVDD72 pKa = 4.41DD73 pKa = 4.36GAVLLYY79 pKa = 10.46FRR81 pKa = 11.84EE82 pKa = 4.21QGTWTAMPYY91 pKa = 9.72TFADD95 pKa = 4.07EE96 pKa = 5.34SPDD99 pKa = 3.73LPAVDD104 pKa = 3.86YY105 pKa = 9.43TITLGYY111 pKa = 10.52GYY113 pKa = 10.05DD114 pKa = 3.62DD115 pKa = 4.13RR116 pKa = 11.84FLEE119 pKa = 4.27VFYY122 pKa = 10.45EE123 pKa = 4.33ASTADD128 pKa = 3.63IPLEE132 pKa = 4.06NQPDD136 pKa = 3.86RR137 pKa = 11.84EE138 pKa = 4.17IKK140 pKa = 10.58AVIIDD145 pKa = 4.1GFPAAKK151 pKa = 10.05AAVDD155 pKa = 4.13FSDD158 pKa = 4.12YY159 pKa = 10.68EE160 pKa = 3.94AVRR163 pKa = 11.84AYY165 pKa = 10.95FNLTDD170 pKa = 3.35

Molecular weight: 18.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0DD06|A0A1V0DD06_9BACT TonB_C domain-containing protein OS=Rhodothermaceae bacterium RA OX=1779382 GN=AWN76_006750 PE=3 SV=1
MM1 pKa = 7.99LIPVLGSTGMGLVWGWLIVQRR22 pKa = 11.84QSHH25 pKa = 6.1RR26 pKa = 11.84PRR28 pKa = 11.84LRR30 pKa = 11.84TAGRR34 pKa = 11.84VLAVVAGTALAAGLNAAWIGPPGLTGFLIATGIALAAHH72 pKa = 6.95LGWLHH77 pKa = 5.33VLHH80 pKa = 6.65RR81 pKa = 11.84QAPPLLSPSRR91 pKa = 3.74

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3503

0

3503

1325449

32

4882

378.4

41.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.514 ± 0.05

0.644 ± 0.01

6.041 ± 0.032

6.104 ± 0.039

3.687 ± 0.027

8.251 ± 0.043

2.344 ± 0.021

4.104 ± 0.03

1.841 ± 0.027

10.771 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.885 ± 0.019

2.291 ± 0.027

6.128 ± 0.033

3.366 ± 0.027

8.428 ± 0.049

4.476 ± 0.029

5.895 ± 0.044

7.802 ± 0.037

1.417 ± 0.022

3.012 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski