Lake Sarah-associated circular virus-49

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAA2|A0A126GAA2_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-49 OX=1685778 PE=4 SV=1
MM1 pKa = 7.6SNAQRR6 pKa = 11.84SGRR9 pKa = 11.84TQGHH13 pKa = 5.65WFIGTCRR20 pKa = 11.84DD21 pKa = 3.64LDD23 pKa = 3.62WDD25 pKa = 4.34GRR27 pKa = 11.84DD28 pKa = 3.19HH29 pKa = 6.78PQFISYY35 pKa = 10.7GKK37 pKa = 9.9GQLEE41 pKa = 4.04LSEE44 pKa = 4.43SGFRR48 pKa = 11.84HH49 pKa = 4.68WQFVVQCKK57 pKa = 10.32DD58 pKa = 2.88STRR61 pKa = 11.84IAKK64 pKa = 9.35LKK66 pKa = 10.2KK67 pKa = 9.23WLPTCHH73 pKa = 6.53WEE75 pKa = 4.09LTRR78 pKa = 11.84SEE80 pKa = 4.23SAIDD84 pKa = 3.89YY85 pKa = 8.62VWKK88 pKa = 10.5EE89 pKa = 3.82EE90 pKa = 3.85TRR92 pKa = 11.84IEE94 pKa = 4.21GSQFEE99 pKa = 4.43FGVRR103 pKa = 11.84RR104 pKa = 11.84LRR106 pKa = 11.84RR107 pKa = 11.84NAATDD112 pKa = 2.92WDD114 pKa = 4.49VIKK117 pKa = 10.85QRR119 pKa = 11.84VLAGSVTSDD128 pKa = 5.34DD129 pKa = 3.45IPADD133 pKa = 3.06IFVRR137 pKa = 11.84YY138 pKa = 9.29YY139 pKa = 10.99SQLKK143 pKa = 9.44YY144 pKa = 10.94NPFYY148 pKa = 10.66YY149 pKa = 10.13RR150 pKa = 11.84SIAKK154 pKa = 10.08DD155 pKa = 3.36YY156 pKa = 10.72SRR158 pKa = 11.84PAAIIRR164 pKa = 11.84SCVVYY169 pKa = 9.98WGPTGTGKK177 pKa = 10.01SRR179 pKa = 11.84RR180 pKa = 11.84AWSEE184 pKa = 3.61ATTAAYY190 pKa = 10.59VKK192 pKa = 10.69DD193 pKa = 4.28PLSKK197 pKa = 9.3WWEE200 pKa = 4.59GYY202 pKa = 9.39QGEE205 pKa = 4.18ISVIIDD211 pKa = 3.4EE212 pKa = 4.68FRR214 pKa = 11.84GIIQPAHH221 pKa = 6.77LLRR224 pKa = 11.84WLDD227 pKa = 3.7RR228 pKa = 11.84YY229 pKa = 9.54PVSVEE234 pKa = 4.09CKK236 pKa = 10.37GGSTPLRR243 pKa = 11.84AQRR246 pKa = 11.84FWICSNLHH254 pKa = 5.62PSQWYY259 pKa = 9.2PEE261 pKa = 4.42LDD263 pKa = 3.3GATYY267 pKa = 10.52SALEE271 pKa = 3.73RR272 pKa = 11.84RR273 pKa = 11.84MEE275 pKa = 4.0IVEE278 pKa = 4.06MAEE281 pKa = 3.38NWEE284 pKa = 4.47PIEE287 pKa = 4.21SEE289 pKa = 3.98EE290 pKa = 4.63LIEE293 pKa = 4.52VSEE296 pKa = 4.24

Molecular weight: 34.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAA2|A0A126GAA2_9VIRU Putative capsid protein OS=Lake Sarah-associated circular virus-49 OX=1685778 PE=4 SV=1
MM1 pKa = 7.61SITRR5 pKa = 11.84RR6 pKa = 11.84HH7 pKa = 5.59SAPSVAYY14 pKa = 8.9STAANIANYY23 pKa = 10.01AIRR26 pKa = 11.84HH27 pKa = 5.02PQQVIQGVRR36 pKa = 11.84RR37 pKa = 11.84LSRR40 pKa = 11.84RR41 pKa = 11.84FSEE44 pKa = 5.04AFSTPQGRR52 pKa = 11.84RR53 pKa = 11.84LRR55 pKa = 11.84SNPIPASGSAPGQSKK70 pKa = 10.28RR71 pKa = 11.84YY72 pKa = 9.09AVSQGRR78 pKa = 11.84SFVGASKK85 pKa = 10.95YY86 pKa = 10.36GGSVIVKK93 pKa = 10.31GGFKK97 pKa = 10.18KK98 pKa = 10.6KK99 pKa = 9.09KK100 pKa = 6.36TFKK103 pKa = 9.63RR104 pKa = 11.84KK105 pKa = 8.6SMKK108 pKa = 10.56RR109 pKa = 11.84MMKK112 pKa = 10.19RR113 pKa = 11.84KK114 pKa = 9.13SKK116 pKa = 10.59KK117 pKa = 9.19GTLTDD122 pKa = 4.06CLTKK126 pKa = 10.4GYY128 pKa = 10.35HH129 pKa = 5.29VTDD132 pKa = 3.8QIYY135 pKa = 10.83GLAQDD140 pKa = 4.43PSAVYY145 pKa = 8.45ITHH148 pKa = 6.58SSWHH152 pKa = 5.95LAQMTRR158 pKa = 11.84VIIGAVLRR166 pKa = 11.84KK167 pKa = 9.5ALRR170 pKa = 11.84KK171 pKa = 10.05AGISVPNKK179 pKa = 8.99DD180 pKa = 3.7TLLPLYY186 pKa = 10.71NDD188 pKa = 3.9GNSGRR193 pKa = 11.84MEE195 pKa = 4.0FQYY198 pKa = 10.56TYY200 pKa = 11.21QNPTSGALAAIVTVPTATGSSFDD223 pKa = 3.75TMLGTLMSSGMGTQIQDD240 pKa = 3.59FLSTVSTNQPHH251 pKa = 6.06SFGVYY256 pKa = 9.35TLDD259 pKa = 4.58SGNQTSMMSRR269 pKa = 11.84IYY271 pKa = 10.31LQNEE275 pKa = 3.88YY276 pKa = 10.57FYY278 pKa = 10.73IPVFSSLKK286 pKa = 8.97FQNRR290 pKa = 11.84TLPATGDD297 pKa = 3.26ATVPNIEE304 pKa = 4.52RR305 pKa = 11.84SDD307 pKa = 3.58VQPIKK312 pKa = 11.05VKK314 pKa = 10.45VFHH317 pKa = 6.87FSAGTPRR324 pKa = 11.84LSFVSTTSATPAVTTNVPMGVVNSNGLFLQTALTIGNVDD363 pKa = 3.65YY364 pKa = 11.22QEE366 pKa = 4.83PPTKK370 pKa = 10.4KK371 pKa = 10.18LWTNCISTSNTVVDD385 pKa = 4.18PGIIKK390 pKa = 10.5YY391 pKa = 10.64SSLKK395 pKa = 8.57YY396 pKa = 8.51TYY398 pKa = 10.6KK399 pKa = 10.25GTLSNLQAKK408 pKa = 8.45LRR410 pKa = 11.84PMKK413 pKa = 10.29YY414 pKa = 8.54DD415 pKa = 2.77TGTTVVTGVAGKK427 pKa = 9.1SQIICIDD434 pKa = 3.34EE435 pKa = 4.14YY436 pKa = 11.64LRR438 pKa = 11.84TTTSNKK444 pKa = 9.44IAVSYY449 pKa = 10.77EE450 pKa = 3.28KK451 pKa = 10.34DD452 pKa = 3.46LRR454 pKa = 11.84IGCYY458 pKa = 9.74CVTKK462 pKa = 10.51KK463 pKa = 10.7EE464 pKa = 3.92PAFTSFLNVPAALNIGG480 pKa = 3.64

Molecular weight: 52.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

776

296

480

388.0

43.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.701 ± 0.173

1.418 ± 0.374

3.995 ± 0.867

4.124 ± 2.448

3.608 ± 0.141

7.088 ± 0.411

1.675 ± 0.216

6.057 ± 0.43

5.928 ± 0.944

6.443 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.644

3.479 ± 1.1

4.897 ± 0.31

4.51 ± 0.073

6.701 ± 1.28

9.794 ± 0.621

8.119 ± 2.082

6.572 ± 0.717

2.191 ± 1.767

4.639 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski