Amino acid dipepetide frequency for Stx2-converting phage Stx2a_WGPS9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.491AlaAla: 9.491 ± 0.91
0.918AlaCys: 0.918 ± 0.316
5.389AlaAsp: 5.389 ± 0.624
8.94AlaGlu: 8.94 ± 0.914
3.858AlaPhe: 3.858 ± 0.684
8.144AlaGly: 8.144 ± 1.268
1.47AlaHis: 1.47 ± 0.397
4.286AlaIle: 4.286 ± 0.586
5.389AlaLys: 5.389 ± 0.673
7.348AlaLeu: 7.348 ± 0.771
3.0AlaMet: 3.0 ± 0.557
2.817AlaAsn: 2.817 ± 0.485
3.123AlaPro: 3.123 ± 0.486
5.511AlaGln: 5.511 ± 0.876
5.94AlaArg: 5.94 ± 0.502
5.94AlaSer: 5.94 ± 0.503
5.633AlaThr: 5.633 ± 0.801
6.491AlaVal: 6.491 ± 0.668
1.408AlaTrp: 1.408 ± 0.251
2.204AlaTyr: 2.204 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
1.163CysAla: 1.163 ± 0.322
0.306CysCys: 0.306 ± 0.166
0.49CysAsp: 0.49 ± 0.198
0.796CysGlu: 0.796 ± 0.241
0.306CysPhe: 0.306 ± 0.149
0.796CysGly: 0.796 ± 0.305
0.306CysHis: 0.306 ± 0.146
0.612CysIle: 0.612 ± 0.241
0.429CysLys: 0.429 ± 0.183
0.796CysLeu: 0.796 ± 0.268
0.122CysMet: 0.122 ± 0.087
0.184CysAsn: 0.184 ± 0.102
0.367CysPro: 0.367 ± 0.171
0.367CysGln: 0.367 ± 0.155
1.163CysArg: 1.163 ± 0.389
0.735CysSer: 0.735 ± 0.258
0.429CysThr: 0.429 ± 0.174
0.735CysVal: 0.735 ± 0.228
0.122CysTrp: 0.122 ± 0.099
0.306CysTyr: 0.306 ± 0.177
0.0CysXaa: 0.0 ± 0.0
Asp
6.062AspAla: 6.062 ± 0.692
0.49AspCys: 0.49 ± 0.208
3.98AspAsp: 3.98 ± 0.5
4.348AspGlu: 4.348 ± 0.601
1.898AspPhe: 1.898 ± 0.38
4.837AspGly: 4.837 ± 0.576
0.918AspHis: 0.918 ± 0.316
3.674AspIle: 3.674 ± 0.37
4.225AspLys: 4.225 ± 0.402
4.103AspLeu: 4.103 ± 0.457
2.021AspMet: 2.021 ± 0.364
2.633AspAsn: 2.633 ± 0.452
2.327AspPro: 2.327 ± 0.469
1.715AspGln: 1.715 ± 0.355
3.429AspArg: 3.429 ± 0.49
3.245AspSer: 3.245 ± 0.464
2.572AspThr: 2.572 ± 0.425
3.796AspVal: 3.796 ± 0.497
1.163AspTrp: 1.163 ± 0.354
1.47AspTyr: 1.47 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
6.674GluAla: 6.674 ± 0.641
0.796GluCys: 0.796 ± 0.272
2.204GluAsp: 2.204 ± 0.299
4.348GluGlu: 4.348 ± 0.469
2.449GluPhe: 2.449 ± 0.443
4.409GluGly: 4.409 ± 0.518
1.347GluHis: 1.347 ± 0.256
3.429GluIle: 3.429 ± 0.428
4.47GluLys: 4.47 ± 0.589
5.511GluLeu: 5.511 ± 0.745
2.021GluMet: 2.021 ± 0.431
3.184GluAsn: 3.184 ± 0.544
1.653GluPro: 1.653 ± 0.412
4.286GluGln: 4.286 ± 0.698
5.878GluArg: 5.878 ± 0.712
4.041GluSer: 4.041 ± 0.448
4.286GluThr: 4.286 ± 0.749
4.409GluVal: 4.409 ± 0.655
1.102GluTrp: 1.102 ± 0.256
2.143GluTyr: 2.143 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
3.184PheAla: 3.184 ± 0.453
0.306PheCys: 0.306 ± 0.144
1.898PheAsp: 1.898 ± 0.316
1.225PheGlu: 1.225 ± 0.32
0.796PhePhe: 0.796 ± 0.289
2.327PheGly: 2.327 ± 0.335
0.612PheHis: 0.612 ± 0.169
1.653PheIle: 1.653 ± 0.269
1.592PheLys: 1.592 ± 0.267
1.898PheLeu: 1.898 ± 0.32
1.163PheMet: 1.163 ± 0.271
1.163PheAsn: 1.163 ± 0.317
1.286PhePro: 1.286 ± 0.336
1.041PheGln: 1.041 ± 0.248
2.327PheArg: 2.327 ± 0.46
2.939PheSer: 2.939 ± 0.46
2.449PheThr: 2.449 ± 0.472
2.449PheVal: 2.449 ± 0.426
0.796PheTrp: 0.796 ± 0.2
0.796PheTyr: 0.796 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
6.429GlyAla: 6.429 ± 1.021
0.674GlyCys: 0.674 ± 0.287
5.082GlyAsp: 5.082 ± 0.824
6.185GlyGlu: 6.185 ± 1.742
2.388GlyPhe: 2.388 ± 0.413
5.082GlyGly: 5.082 ± 0.832
0.98GlyHis: 0.98 ± 0.273
3.552GlyIle: 3.552 ± 0.613
4.715GlyLys: 4.715 ± 1.009
4.96GlyLeu: 4.96 ± 0.439
2.327GlyMet: 2.327 ± 0.408
3.062GlyAsn: 3.062 ± 0.394
4.592GlyPro: 4.592 ± 2.645
2.817GlyGln: 2.817 ± 0.501
4.164GlyArg: 4.164 ± 0.494
3.98GlySer: 3.98 ± 0.529
3.735GlyThr: 3.735 ± 0.535
5.327GlyVal: 5.327 ± 0.575
1.408GlyTrp: 1.408 ± 0.263
2.633GlyTyr: 2.633 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.592HisAla: 1.592 ± 0.288
0.245HisCys: 0.245 ± 0.143
1.041HisAsp: 1.041 ± 0.21
0.857HisGlu: 0.857 ± 0.247
0.674HisPhe: 0.674 ± 0.262
1.347HisGly: 1.347 ± 0.396
0.367HisHis: 0.367 ± 0.159
0.612HisIle: 0.612 ± 0.194
0.735HisLys: 0.735 ± 0.232
1.408HisLeu: 1.408 ± 0.481
0.245HisMet: 0.245 ± 0.114
0.918HisAsn: 0.918 ± 0.203
0.857HisPro: 0.857 ± 0.262
0.551HisGln: 0.551 ± 0.2
0.796HisArg: 0.796 ± 0.273
0.796HisSer: 0.796 ± 0.2
0.918HisThr: 0.918 ± 0.276
0.735HisVal: 0.735 ± 0.229
0.306HisTrp: 0.306 ± 0.174
0.735HisTyr: 0.735 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
5.205IleAla: 5.205 ± 0.592
0.857IleCys: 0.857 ± 0.284
3.184IleAsp: 3.184 ± 0.485
3.123IleGlu: 3.123 ± 0.509
1.041IlePhe: 1.041 ± 0.227
2.633IleGly: 2.633 ± 0.544
0.796IleHis: 0.796 ± 0.185
2.266IleIle: 2.266 ± 0.424
2.939IleLys: 2.939 ± 0.395
3.123IleLeu: 3.123 ± 0.64
1.041IleMet: 1.041 ± 0.238
2.878IleAsn: 2.878 ± 0.457
2.511IlePro: 2.511 ± 0.37
2.021IleGln: 2.021 ± 0.298
4.654IleArg: 4.654 ± 0.463
3.919IleSer: 3.919 ± 0.675
3.49IleThr: 3.49 ± 0.658
2.327IleVal: 2.327 ± 0.428
0.49IleTrp: 0.49 ± 0.206
1.286IleTyr: 1.286 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
6.246LysAla: 6.246 ± 0.79
0.429LysCys: 0.429 ± 0.23
3.123LysAsp: 3.123 ± 0.518
3.429LysGlu: 3.429 ± 0.469
1.102LysPhe: 1.102 ± 0.237
5.45LysGly: 5.45 ± 1.115
0.796LysHis: 0.796 ± 0.235
3.307LysIle: 3.307 ± 0.458
3.368LysLys: 3.368 ± 0.539
5.021LysLeu: 5.021 ± 0.577
2.143LysMet: 2.143 ± 0.373
3.245LysAsn: 3.245 ± 0.395
2.388LysPro: 2.388 ± 0.4
3.245LysGln: 3.245 ± 0.638
2.939LysArg: 2.939 ± 0.406
3.0LysSer: 3.0 ± 0.416
3.552LysThr: 3.552 ± 0.456
3.552LysVal: 3.552 ± 0.491
0.612LysTrp: 0.612 ± 0.207
1.347LysTyr: 1.347 ± 0.327
0.0LysXaa: 0.0 ± 0.0
Leu
8.389LeuAla: 8.389 ± 0.902
1.102LeuCys: 1.102 ± 0.299
4.103LeuAsp: 4.103 ± 0.61
4.164LeuGlu: 4.164 ± 0.623
2.511LeuPhe: 2.511 ± 0.42
4.531LeuGly: 4.531 ± 0.566
1.347LeuHis: 1.347 ± 0.286
3.735LeuIle: 3.735 ± 0.608
4.531LeuLys: 4.531 ± 0.575
6.307LeuLeu: 6.307 ± 0.551
2.082LeuMet: 2.082 ± 0.399
4.225LeuAsn: 4.225 ± 0.532
3.98LeuPro: 3.98 ± 0.488
3.062LeuGln: 3.062 ± 0.83
5.082LeuArg: 5.082 ± 0.599
4.592LeuSer: 4.592 ± 0.455
4.776LeuThr: 4.776 ± 0.461
4.96LeuVal: 4.96 ± 0.477
0.551LeuTrp: 0.551 ± 0.24
2.204LeuTyr: 2.204 ± 0.372
0.0LeuXaa: 0.0 ± 0.0
Met
3.0MetAla: 3.0 ± 0.473
0.061MetCys: 0.061 ± 0.062
1.531MetAsp: 1.531 ± 0.247
1.592MetGlu: 1.592 ± 0.266
0.612MetPhe: 0.612 ± 0.207
1.531MetGly: 1.531 ± 0.357
0.245MetHis: 0.245 ± 0.109
0.735MetIle: 0.735 ± 0.215
2.143MetLys: 2.143 ± 0.329
1.959MetLeu: 1.959 ± 0.337
0.674MetMet: 0.674 ± 0.214
1.715MetAsn: 1.715 ± 0.364
1.531MetPro: 1.531 ± 0.338
1.531MetGln: 1.531 ± 0.279
1.408MetArg: 1.408 ± 0.284
2.082MetSer: 2.082 ± 0.348
2.817MetThr: 2.817 ± 0.384
1.531MetVal: 1.531 ± 0.389
0.184MetTrp: 0.184 ± 0.112
0.551MetTyr: 0.551 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
4.837AsnAla: 4.837 ± 0.769
0.306AsnCys: 0.306 ± 0.158
2.266AsnAsp: 2.266 ± 0.431
3.245AsnGlu: 3.245 ± 0.446
1.47AsnPhe: 1.47 ± 0.346
3.307AsnGly: 3.307 ± 0.552
1.041AsnHis: 1.041 ± 0.326
2.511AsnIle: 2.511 ± 0.413
2.143AsnLys: 2.143 ± 0.378
3.429AsnLeu: 3.429 ± 0.478
1.347AsnMet: 1.347 ± 0.271
2.143AsnAsn: 2.143 ± 0.493
1.592AsnPro: 1.592 ± 0.285
2.204AsnGln: 2.204 ± 0.395
3.062AsnArg: 3.062 ± 0.557
2.449AsnSer: 2.449 ± 0.446
2.388AsnThr: 2.388 ± 0.449
2.204AsnVal: 2.204 ± 0.384
0.551AsnTrp: 0.551 ± 0.153
1.163AsnTyr: 1.163 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
3.552ProAla: 3.552 ± 0.679
0.367ProCys: 0.367 ± 0.148
4.225ProAsp: 4.225 ± 0.548
4.96ProGlu: 4.96 ± 0.888
1.225ProPhe: 1.225 ± 0.23
3.062ProGly: 3.062 ± 0.637
0.306ProHis: 0.306 ± 0.125
0.796ProIle: 0.796 ± 0.217
2.817ProLys: 2.817 ± 0.788
2.633ProLeu: 2.633 ± 0.369
0.98ProMet: 0.98 ± 0.207
1.347ProAsn: 1.347 ± 0.355
1.286ProPro: 1.286 ± 0.305
2.633ProGln: 2.633 ± 0.611
2.021ProArg: 2.021 ± 0.418
2.511ProSer: 2.511 ± 0.387
1.531ProThr: 1.531 ± 0.343
4.531ProVal: 4.531 ± 0.483
0.735ProTrp: 0.735 ± 0.222
1.653ProTyr: 1.653 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
4.837GlnAla: 4.837 ± 0.713
0.674GlnCys: 0.674 ± 0.207
2.755GlnAsp: 2.755 ± 0.367
3.0GlnGlu: 3.0 ± 0.501
1.837GlnPhe: 1.837 ± 0.318
3.674GlnGly: 3.674 ± 0.899
0.857GlnHis: 0.857 ± 0.198
2.143GlnIle: 2.143 ± 0.444
3.245GlnLys: 3.245 ± 0.572
3.674GlnLeu: 3.674 ± 0.452
1.041GlnMet: 1.041 ± 0.289
1.898GlnAsn: 1.898 ± 0.426
1.898GlnPro: 1.898 ± 0.448
4.286GlnGln: 4.286 ± 0.961
3.552GlnArg: 3.552 ± 0.739
2.449GlnSer: 2.449 ± 0.365
2.204GlnThr: 2.204 ± 0.398
2.633GlnVal: 2.633 ± 0.599
0.735GlnTrp: 0.735 ± 0.235
1.47GlnTyr: 1.47 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
4.348ArgAla: 4.348 ± 0.435
0.49ArgCys: 0.49 ± 0.173
4.164ArgAsp: 4.164 ± 0.695
5.572ArgGlu: 5.572 ± 0.711
2.511ArgPhe: 2.511 ± 0.418
5.205ArgGly: 5.205 ± 1.118
1.592ArgHis: 1.592 ± 0.333
3.858ArgIle: 3.858 ± 0.552
4.041ArgLys: 4.041 ± 0.432
5.389ArgLeu: 5.389 ± 0.516
1.837ArgMet: 1.837 ± 0.287
3.123ArgAsn: 3.123 ± 0.399
2.388ArgPro: 2.388 ± 0.427
3.307ArgGln: 3.307 ± 0.526
5.633ArgArg: 5.633 ± 0.703
3.552ArgSer: 3.552 ± 0.473
3.552ArgThr: 3.552 ± 0.656
4.348ArgVal: 4.348 ± 0.583
1.163ArgTrp: 1.163 ± 0.285
1.959ArgTyr: 1.959 ± 0.514
0.0ArgXaa: 0.0 ± 0.0
Ser
5.878SerAla: 5.878 ± 0.625
0.674SerCys: 0.674 ± 0.236
3.674SerAsp: 3.674 ± 0.444
4.225SerGlu: 4.225 ± 0.459
1.47SerPhe: 1.47 ± 0.269
5.45SerGly: 5.45 ± 0.645
0.735SerHis: 0.735 ± 0.189
2.327SerIle: 2.327 ± 0.343
2.817SerLys: 2.817 ± 0.582
5.511SerLeu: 5.511 ± 0.653
1.408SerMet: 1.408 ± 0.292
2.694SerAsn: 2.694 ± 0.438
3.429SerPro: 3.429 ± 0.443
3.429SerGln: 3.429 ± 0.406
3.735SerArg: 3.735 ± 0.645
3.184SerSer: 3.184 ± 0.723
3.674SerThr: 3.674 ± 0.529
3.796SerVal: 3.796 ± 0.564
0.98SerTrp: 0.98 ± 0.237
1.837SerTyr: 1.837 ± 0.435
0.0SerXaa: 0.0 ± 0.0
Thr
5.817ThrAla: 5.817 ± 0.561
0.49ThrCys: 0.49 ± 0.192
3.245ThrAsp: 3.245 ± 0.413
3.674ThrGlu: 3.674 ± 0.513
1.837ThrPhe: 1.837 ± 0.411
6.062ThrGly: 6.062 ± 1.019
0.857ThrHis: 0.857 ± 0.237
3.796ThrIle: 3.796 ± 0.56
2.817ThrLys: 2.817 ± 0.481
4.47ThrLeu: 4.47 ± 0.554
0.796ThrMet: 0.796 ± 0.188
1.715ThrAsn: 1.715 ± 0.282
3.49ThrPro: 3.49 ± 0.4
1.837ThrGln: 1.837 ± 0.359
2.939ThrArg: 2.939 ± 0.4
3.858ThrSer: 3.858 ± 0.597
3.184ThrThr: 3.184 ± 0.454
4.348ThrVal: 4.348 ± 0.545
0.857ThrTrp: 0.857 ± 0.206
1.347ThrTyr: 1.347 ± 0.317
0.0ThrXaa: 0.0 ± 0.0
Val
7.042ValAla: 7.042 ± 0.574
0.98ValCys: 0.98 ± 0.279
4.164ValAsp: 4.164 ± 0.537
3.245ValGlu: 3.245 ± 0.427
2.021ValPhe: 2.021 ± 0.347
3.307ValGly: 3.307 ± 0.539
0.551ValHis: 0.551 ± 0.169
3.98ValIle: 3.98 ± 0.571
3.49ValLys: 3.49 ± 0.374
5.817ValLeu: 5.817 ± 0.72
1.776ValMet: 1.776 ± 0.322
3.062ValAsn: 3.062 ± 0.401
2.572ValPro: 2.572 ± 0.363
2.327ValGln: 2.327 ± 0.36
5.144ValArg: 5.144 ± 1.135
5.144ValSer: 5.144 ± 0.656
4.103ValThr: 4.103 ± 0.503
4.225ValVal: 4.225 ± 0.537
0.612ValTrp: 0.612 ± 0.181
1.959ValTyr: 1.959 ± 0.362
0.0ValXaa: 0.0 ± 0.0
Trp
0.796TrpAla: 0.796 ± 0.26
0.245TrpCys: 0.245 ± 0.119
0.429TrpAsp: 0.429 ± 0.17
0.429TrpGlu: 0.429 ± 0.136
0.612TrpPhe: 0.612 ± 0.159
0.612TrpGly: 0.612 ± 0.209
0.122TrpHis: 0.122 ± 0.081
0.857TrpIle: 0.857 ± 0.186
1.347TrpLys: 1.347 ± 0.289
1.408TrpLeu: 1.408 ± 0.441
0.674TrpMet: 0.674 ± 0.203
0.551TrpAsn: 0.551 ± 0.179
0.612TrpPro: 0.612 ± 0.238
1.225TrpGln: 1.225 ± 0.218
1.531TrpArg: 1.531 ± 0.351
1.041TrpSer: 1.041 ± 0.227
0.49TrpThr: 0.49 ± 0.19
1.041TrpVal: 1.041 ± 0.298
0.367TrpTrp: 0.367 ± 0.2
0.429TrpTyr: 0.429 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.817TyrAla: 2.817 ± 0.461
0.122TyrCys: 0.122 ± 0.094
1.959TyrAsp: 1.959 ± 0.38
1.286TyrGlu: 1.286 ± 0.328
1.286TyrPhe: 1.286 ± 0.248
2.327TyrGly: 2.327 ± 0.49
0.49TyrHis: 0.49 ± 0.173
1.653TyrIle: 1.653 ± 0.304
1.041TyrLys: 1.041 ± 0.267
1.47TyrLeu: 1.47 ± 0.294
0.674TyrMet: 0.674 ± 0.218
1.102TyrAsn: 1.102 ± 0.235
1.286TyrPro: 1.286 ± 0.27
1.47TyrGln: 1.47 ± 0.251
2.633TyrArg: 2.633 ± 0.488
1.47TyrSer: 1.47 ± 0.309
1.653TyrThr: 1.653 ± 0.462
2.082TyrVal: 2.082 ± 0.355
0.674TyrTrp: 0.674 ± 0.172
1.225TyrTyr: 1.225 ± 0.255
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (16332 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski