bacterium HR10

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5W159|A0A2H5W159_9BACT Multifunctional non-homologous end joining protein LigD OS=bacterium HR10 OX=2035405 GN=ligD PE=4 SV=1
MM1 pKa = 7.15ATYY4 pKa = 10.39PEE6 pKa = 4.81DD7 pKa = 3.32CLYY10 pKa = 10.6TRR12 pKa = 11.84EE13 pKa = 4.36HH14 pKa = 5.04EE15 pKa = 4.52WIRR18 pKa = 11.84VEE20 pKa = 5.13DD21 pKa = 3.98DD22 pKa = 3.38VGVIGITDD30 pKa = 3.67YY31 pKa = 11.46AQEE34 pKa = 4.01ALGDD38 pKa = 3.67IVYY41 pKa = 10.47VEE43 pKa = 4.55LPSVGDD49 pKa = 3.77HH50 pKa = 6.7FSQGEE55 pKa = 3.81PFGNVEE61 pKa = 4.09SVKK64 pKa = 10.59AVSEE68 pKa = 4.31LYY70 pKa = 9.75MPVSGEE76 pKa = 3.91VVEE79 pKa = 4.47INEE82 pKa = 4.75DD83 pKa = 3.66LAEE86 pKa = 4.38SPQLVNEE93 pKa = 4.48DD94 pKa = 4.09PYY96 pKa = 11.74GDD98 pKa = 3.09GWMVKK103 pKa = 8.26ITLSDD108 pKa = 3.52PTEE111 pKa = 4.52LEE113 pKa = 4.2TLMSASEE120 pKa = 3.94YY121 pKa = 10.18EE122 pKa = 4.23EE123 pKa = 4.07YY124 pKa = 11.02VKK126 pKa = 10.71EE127 pKa = 3.97EE128 pKa = 3.99RR129 pKa = 11.84QKK131 pKa = 11.38EE132 pKa = 4.19KK133 pKa = 11.19

Molecular weight: 15.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5VXW4|A0A2H5VXW4_9BACT Dihydroorotase OS=bacterium HR10 OX=2035405 GN=pyrC PE=3 SV=1
MM1 pKa = 7.82PNPKK5 pKa = 9.2RR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 5.15SKK10 pKa = 9.1SRR12 pKa = 11.84RR13 pKa = 11.84GKK15 pKa = 10.1RR16 pKa = 11.84RR17 pKa = 11.84AHH19 pKa = 6.88DD20 pKa = 3.72ALMPPNLTTCSQCRR34 pKa = 11.84EE35 pKa = 3.91PVLPHH40 pKa = 6.37RR41 pKa = 11.84VCPRR45 pKa = 11.84CGYY48 pKa = 9.09YY49 pKa = 10.1NGRR52 pKa = 11.84EE53 pKa = 4.05VIPVKK58 pKa = 10.34EE59 pKa = 3.99

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2687

0

2687

941310

29

1727

350.3

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.592 ± 0.06

0.866 ± 0.017

4.477 ± 0.032

7.441 ± 0.051

3.852 ± 0.034

7.931 ± 0.047

2.21 ± 0.021

5.322 ± 0.034

2.409 ± 0.031

11.002 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.019

2.144 ± 0.037

5.542 ± 0.036

3.035 ± 0.031

9.83 ± 0.052

4.676 ± 0.033

4.947 ± 0.032

7.772 ± 0.039

1.45 ± 0.023

2.599 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski