Amino acid dipepetide frequency for Thamnophis sirtalis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.868AlaAla: 5.868 ± 0.044	1.252AlaCys: 1.252 ± 0.012	2.779AlaAsp: 2.779 ± 0.016	4.526AlaGlu: 4.526 ± 0.03	2.554AlaPhe: 2.554 ± 0.016	3.8AlaGly: 3.8 ± 0.024	1.371AlaHis: 1.371 ± 0.011	3.061AlaIle: 3.061 ± 0.019	3.681AlaLys: 3.681 ± 0.025	6.316AlaLeu: 6.316 ± 0.032	1.468AlaMet: 1.468 ± 0.012	2.28AlaAsn: 2.28 ± 0.015	3.147AlaPro: 3.147 ± 0.025	2.785AlaGln: 2.785 ± 0.018	2.911AlaArg: 2.911 ± 0.021	5.477AlaSer: 5.477 ± 0.029	3.472AlaThr: 3.472 ± 0.022	4.284AlaVal: 4.284 ± 0.021	0.671AlaTrp: 0.671 ± 0.009	1.511AlaTyr: 1.511 ± 0.012	0.002AlaXaa: 0.002 ± 0.0
Cys	1.089CysAla: 1.089 ± 0.011	0.59CysCys: 0.59 ± 0.01	1.003CysAsp: 1.003 ± 0.014	1.247CysGlu: 1.247 ± 0.016	0.919CysPhe: 0.919 ± 0.011	1.652CysGly: 1.652 ± 0.027	0.652CysHis: 0.652 ± 0.008	1.138CysIle: 1.138 ± 0.011	1.238CysLys: 1.238 ± 0.014	2.211CysLeu: 2.211 ± 0.017	0.489CysMet: 0.489 ± 0.009	0.947CysAsn: 0.947 ± 0.011	1.27CysPro: 1.27 ± 0.017	1.071CysGln: 1.071 ± 0.012	1.151CysArg: 1.151 ± 0.013	2.028CysSer: 2.028 ± 0.016	1.139CysThr: 1.139 ± 0.012	1.186CysVal: 1.186 ± 0.013	0.269CysTrp: 0.269 ± 0.005	0.651CysTyr: 0.651 ± 0.008	0.001CysXaa: 0.001 ± 0.0
Asp	2.726AspAla: 2.726 ± 0.016	1.132AspCys: 1.132 ± 0.015	2.741AspAsp: 2.741 ± 0.019	3.529AspGlu: 3.529 ± 0.021	2.19AspPhe: 2.19 ± 0.014	3.135AspGly: 3.135 ± 0.024	1.178AspHis: 1.178 ± 0.01	2.913AspIle: 2.913 ± 0.02	2.688AspLys: 2.688 ± 0.018	4.953AspLeu: 4.953 ± 0.025	1.143AspMet: 1.143 ± 0.01	1.95AspAsn: 1.95 ± 0.015	2.636AspPro: 2.636 ± 0.019	1.902AspGln: 1.902 ± 0.014	2.256AspArg: 2.256 ± 0.016	4.216AspSer: 4.216 ± 0.023	2.39AspThr: 2.39 ± 0.017	2.936AspVal: 2.936 ± 0.021	0.604AspTrp: 0.604 ± 0.008	1.56AspTyr: 1.56 ± 0.012	0.001AspXaa: 0.001 ± 0.0
Glu	4.77GluAla: 4.77 ± 0.03	1.485GluCys: 1.485 ± 0.024	4.455GluAsp: 4.455 ± 0.024	8.238GluGlu: 8.238 ± 0.063	2.129GluPhe: 2.129 ± 0.016	3.846GluGly: 3.846 ± 0.024	1.549GluHis: 1.549 ± 0.013	3.734GluIle: 3.734 ± 0.019	6.099GluLys: 6.099 ± 0.047	6.307GluLeu: 6.307 ± 0.036	1.882GluMet: 1.882 ± 0.015	3.532GluAsn: 3.532 ± 0.024	2.772GluPro: 2.772 ± 0.032	3.281GluGln: 3.281 ± 0.027	3.949GluArg: 3.949 ± 0.032	4.718GluSer: 4.718 ± 0.026	3.643GluThr: 3.643 ± 0.02	4.008GluVal: 4.008 ± 0.024	0.722GluTrp: 0.722 ± 0.008	1.679GluTyr: 1.679 ± 0.017	0.002GluXaa: 0.002 ± 0.0
Phe	2.091PheAla: 2.091 ± 0.016	1.008PheCys: 1.008 ± 0.01	1.729PheAsp: 1.729 ± 0.012	2.106PheGlu: 2.106 ± 0.016	1.701PhePhe: 1.701 ± 0.018	2.217PheGly: 2.217 ± 0.017	1.11PheHis: 1.11 ± 0.009	2.043PheIle: 2.043 ± 0.018	1.945PheLys: 1.945 ± 0.016	4.259PheLeu: 4.259 ± 0.03	0.841PheMet: 0.841 ± 0.01	1.53PheAsn: 1.53 ± 0.012	2.065PhePro: 2.065 ± 0.016	1.94PheGln: 1.94 ± 0.013	1.874PheArg: 1.874 ± 0.015	3.645PheSer: 3.645 ± 0.021	2.058PheThr: 2.058 ± 0.016	2.173PheVal: 2.173 ± 0.015	0.49PheTrp: 0.49 ± 0.007	1.252PheTyr: 1.252 ± 0.012	0.002PheXaa: 0.002 ± 0.0
Gly	3.467GlyAla: 3.467 ± 0.025	1.162GlyCys: 1.162 ± 0.012	2.87GlyAsp: 2.87 ± 0.021	3.977GlyGlu: 3.977 ± 0.03	2.372GlyPhe: 2.372 ± 0.017	4.296GlyGly: 4.296 ± 0.036	1.509GlyHis: 1.509 ± 0.013	3.064GlyIle: 3.064 ± 0.018	4.099GlyLys: 4.099 ± 0.028	5.188GlyLeu: 5.188 ± 0.029	1.359GlyMet: 1.359 ± 0.012	2.645GlyAsn: 2.645 ± 0.018	2.937GlyPro: 2.937 ± 0.037	2.542GlyGln: 2.542 ± 0.019	3.155GlyArg: 3.155 ± 0.016	5.334GlySer: 5.334 ± 0.031	3.338GlyThr: 3.338 ± 0.024	3.076GlyVal: 3.076 ± 0.02	0.713GlyTrp: 0.713 ± 0.009	1.746GlyTyr: 1.746 ± 0.017	0.002GlyXaa: 0.002 ± 0.0
His	1.279HisAla: 1.279 ± 0.011	0.699HisCys: 0.699 ± 0.009	0.911HisAsp: 0.911 ± 0.01	1.403HisGlu: 1.403 ± 0.013	1.153HisPhe: 1.153 ± 0.009	1.419HisGly: 1.419 ± 0.011	0.943HisHis: 0.943 ± 0.014	1.371HisIle: 1.371 ± 0.013	1.523HisLys: 1.523 ± 0.018	2.961HisLeu: 2.961 ± 0.02	0.592HisMet: 0.592 ± 0.008	1.043HisAsn: 1.043 ± 0.009	1.592HisPro: 1.592 ± 0.013	1.431HisGln: 1.431 ± 0.018	1.457HisArg: 1.457 ± 0.013	2.386HisSer: 2.386 ± 0.016	1.535HisThr: 1.535 ± 0.023	1.37HisVal: 1.37 ± 0.011	0.328HisTrp: 0.328 ± 0.006	0.84HisTyr: 0.84 ± 0.01	0.002HisXaa: 0.002 ± 0.0
Ile	2.918IleAla: 2.918 ± 0.018	1.285IleCys: 1.285 ± 0.014	2.31IleAsp: 2.31 ± 0.018	2.956IleGlu: 2.956 ± 0.021	2.162IlePhe: 2.162 ± 0.017	2.528IleGly: 2.528 ± 0.017	1.633IleHis: 1.633 ± 0.023	2.848IleIle: 2.848 ± 0.02	3.095IleLys: 3.095 ± 0.019	5.1IleLeu: 5.1 ± 0.027	1.137IleMet: 1.137 ± 0.011	2.25IleAsn: 2.25 ± 0.018	2.935IlePro: 2.935 ± 0.018	2.648IleGln: 2.648 ± 0.014	2.474IleArg: 2.474 ± 0.016	4.363IleSer: 4.363 ± 0.024	2.855IleThr: 2.855 ± 0.024	2.864IleVal: 2.864 ± 0.019	0.573IleTrp: 0.573 ± 0.008	1.579IleTyr: 1.579 ± 0.014	0.002IleXaa: 0.002 ± 0.0
Lys	3.994LysAla: 3.994 ± 0.024	1.394LysCys: 1.394 ± 0.022	3.382LysAsp: 3.382 ± 0.024	5.8LysGlu: 5.8 ± 0.042	1.818LysPhe: 1.818 ± 0.013	3.364LysGly: 3.364 ± 0.027	1.626LysHis: 1.626 ± 0.014	3.308LysIle: 3.308 ± 0.022	5.391LysLys: 5.391 ± 0.041	5.6LysLeu: 5.6 ± 0.03	1.671LysMet: 1.671 ± 0.015	2.869LysAsn: 2.869 ± 0.018	3.273LysPro: 3.273 ± 0.031	2.954LysGln: 2.954 ± 0.02	3.644LysArg: 3.644 ± 0.026	4.47LysSer: 4.47 ± 0.029	3.454LysThr: 3.454 ± 0.022	3.577LysVal: 3.577 ± 0.027	0.657LysTrp: 0.657 ± 0.009	1.733LysTyr: 1.733 ± 0.016	0.003LysXaa: 0.003 ± 0.001
Leu	6.304LeuAla: 6.304 ± 0.033	2.088LeuCys: 2.088 ± 0.018	4.727LeuAsp: 4.727 ± 0.023	7.172LeuGlu: 7.172 ± 0.039	3.469LeuPhe: 3.469 ± 0.025	5.189LeuGly: 5.189 ± 0.026	2.791LeuHis: 2.791 ± 0.021	4.311LeuIle: 4.311 ± 0.027	6.34LeuLys: 6.34 ± 0.035	10.248LeuLeu: 10.248 ± 0.059	2.104LeuMet: 2.104 ± 0.015	3.983LeuAsn: 3.983 ± 0.024	5.688LeuPro: 5.688 ± 0.031	5.791LeuGln: 5.791 ± 0.031	5.137LeuArg: 5.137 ± 0.024	8.054LeuSer: 8.054 ± 0.034	4.896LeuThr: 4.896 ± 0.026	5.151LeuVal: 5.151 ± 0.024	1.045LeuTrp: 1.045 ± 0.011	2.576LeuTyr: 2.576 ± 0.017	0.005LeuXaa: 0.005 ± 0.001
Met	1.842MetAla: 1.842 ± 0.013	0.403MetCys: 0.403 ± 0.006	1.359MetAsp: 1.359 ± 0.011	2.138MetGlu: 2.138 ± 0.017	0.823MetPhe: 0.823 ± 0.009	1.298MetGly: 1.298 ± 0.011	0.522MetHis: 0.522 ± 0.007	1.003MetIle: 1.003 ± 0.011	1.655MetLys: 1.655 ± 0.011	2.098MetLeu: 2.098 ± 0.015	0.62MetMet: 0.62 ± 0.007	1.021MetAsn: 1.021 ± 0.011	1.156MetPro: 1.156 ± 0.011	1.055MetGln: 1.055 ± 0.01	1.03MetArg: 1.03 ± 0.009	1.658MetSer: 1.658 ± 0.012	1.165MetThr: 1.165 ± 0.01	1.414MetVal: 1.414 ± 0.012	0.234MetTrp: 0.234 ± 0.005	0.63MetTyr: 0.63 ± 0.007	0.001MetXaa: 0.001 ± 0.0
Asn	2.301AsnAla: 2.301 ± 0.015	0.982AsnCys: 0.982 ± 0.011	1.747AsnAsp: 1.747 ± 0.016	2.697AsnGlu: 2.697 ± 0.02	1.643AsnPhe: 1.643 ± 0.014	2.752AsnGly: 2.752 ± 0.023	1.094AsnHis: 1.094 ± 0.012	2.637AsnIle: 2.637 ± 0.017	2.595AsnLys: 2.595 ± 0.019	4.206AsnLeu: 4.206 ± 0.022	1.056AsnMet: 1.056 ± 0.01	1.972AsnAsn: 1.972 ± 0.017	2.378AsnPro: 2.378 ± 0.017	1.952AsnGln: 1.952 ± 0.015	2.038AsnArg: 2.038 ± 0.013	3.771AsnSer: 3.771 ± 0.026	2.21AsnThr: 2.21 ± 0.016	2.408AsnVal: 2.408 ± 0.014	0.513AsnTrp: 0.513 ± 0.006	1.302AsnTyr: 1.302 ± 0.01	0.001AsnXaa: 0.001 ± 0.0
Pro	3.755ProAla: 3.755 ± 0.029	1.019ProCys: 1.019 ± 0.011	2.612ProAsp: 2.612 ± 0.016	3.974ProGlu: 3.974 ± 0.032	2.058ProPhe: 2.058 ± 0.014	3.853ProGly: 3.853 ± 0.042	1.352ProHis: 1.352 ± 0.014	2.174ProIle: 2.174 ± 0.018	2.935ProLys: 2.935 ± 0.03	4.996ProLeu: 4.996 ± 0.027	1.048ProMet: 1.048 ± 0.011	2.073ProAsn: 2.073 ± 0.015	5.195ProPro: 5.195 ± 0.056	2.542ProGln: 2.542 ± 0.019	2.601ProArg: 2.601 ± 0.019	5.475ProSer: 5.475 ± 0.033	2.912ProThr: 2.912 ± 0.022	3.577ProVal: 3.577 ± 0.027	0.573ProTrp: 0.573 ± 0.008	1.614ProTyr: 1.614 ± 0.022	0.003ProXaa: 0.003 ± 0.0
Gln	3.156GlnAla: 3.156 ± 0.023	0.963GlnCys: 0.963 ± 0.012	2.254GlnAsp: 2.254 ± 0.015	3.913GlnGlu: 3.913 ± 0.03	1.496GlnPhe: 1.496 ± 0.011	2.538GlnGly: 2.538 ± 0.018	1.335GlnHis: 1.335 ± 0.013	2.353GlnIle: 2.353 ± 0.016	3.264GlnLys: 3.264 ± 0.023	4.759GlnLeu: 4.759 ± 0.03	1.22GlnMet: 1.22 ± 0.012	2.27GlnAsn: 2.27 ± 0.016	2.625GlnPro: 2.625 ± 0.024	3.367GlnGln: 3.367 ± 0.042	2.815GlnArg: 2.815 ± 0.019	3.315GlnSer: 3.315 ± 0.019	2.44GlnThr: 2.44 ± 0.017	2.661GlnVal: 2.661 ± 0.016	0.523GlnTrp: 0.523 ± 0.006	1.241GlnTyr: 1.241 ± 0.012	0.002GlnXaa: 0.002 ± 0.0
Arg	2.929ArgAla: 2.929 ± 0.02	1.078ArgCys: 1.078 ± 0.015	2.505ArgAsp: 2.505 ± 0.019	3.628ArgGlu: 3.628 ± 0.026	1.863ArgPhe: 1.863 ± 0.014	2.835ArgGly: 2.835 ± 0.022	1.453ArgHis: 1.453 ± 0.014	2.706ArgIle: 2.706 ± 0.015	3.838ArgLys: 3.838 ± 0.022	4.785ArgLeu: 4.785 ± 0.025	1.237ArgMet: 1.237 ± 0.012	2.306ArgAsn: 2.306 ± 0.014	2.56ArgPro: 2.56 ± 0.02	2.505ArgGln: 2.505 ± 0.018	3.761ArgArg: 3.761 ± 0.027	4.098ArgSer: 4.098 ± 0.029	2.766ArgThr: 2.766 ± 0.018	2.623ArgVal: 2.623 ± 0.02	0.623ArgTrp: 0.623 ± 0.007	1.462ArgTyr: 1.462 ± 0.013	0.003ArgXaa: 0.003 ± 0.0
Ser	5.132SerAla: 5.132 ± 0.024	1.841SerCys: 1.841 ± 0.015	4.056SerAsp: 4.056 ± 0.021	5.47SerGlu: 5.47 ± 0.03	3.4SerPhe: 3.4 ± 0.021	5.212SerGly: 5.212 ± 0.028	2.255SerHis: 2.255 ± 0.019	3.789SerIle: 3.789 ± 0.02	4.597SerLys: 4.597 ± 0.025	8.266SerLeu: 8.266 ± 0.035	1.749SerMet: 1.749 ± 0.012	3.339SerAsn: 3.339 ± 0.02	5.707SerPro: 5.707 ± 0.036	3.98SerGln: 3.98 ± 0.022	4.251SerArg: 4.251 ± 0.028	10.042SerSer: 10.042 ± 0.063	4.78SerThr: 4.78 ± 0.025	4.879SerVal: 4.879 ± 0.023	0.978SerTrp: 0.978 ± 0.01	2.228SerTyr: 2.228 ± 0.017	0.003SerXaa: 0.003 ± 0.001
Thr	3.562ThrAla: 3.562 ± 0.021	1.263ThrCys: 1.263 ± 0.017	2.534ThrAsp: 2.534 ± 0.017	3.738ThrGlu: 3.738 ± 0.023	2.236ThrPhe: 2.236 ± 0.017	3.514ThrGly: 3.514 ± 0.027	1.297ThrHis: 1.297 ± 0.014	2.761ThrIle: 2.761 ± 0.02	2.905ThrLys: 2.905 ± 0.02	5.137ThrLeu: 5.137 ± 0.023	1.202ThrMet: 1.202 ± 0.009	2.016ThrAsn: 2.016 ± 0.015	3.264ThrPro: 3.264 ± 0.026	2.201ThrGln: 2.201 ± 0.016	2.253ThrArg: 2.253 ± 0.015	5.02ThrSer: 5.02 ± 0.028	3.153ThrThr: 3.153 ± 0.022	3.673ThrVal: 3.673 ± 0.029	0.626ThrTrp: 0.626 ± 0.009	1.444ThrTyr: 1.444 ± 0.012	0.002ThrXaa: 0.002 ± 0.0
Val	3.944ValAla: 3.944 ± 0.021	1.346ValCys: 1.346 ± 0.014	2.817ValAsp: 2.817 ± 0.017	3.809ValGlu: 3.809 ± 0.027	2.34ValPhe: 2.34 ± 0.016	3.113ValGly: 3.113 ± 0.018	1.472ValHis: 1.472 ± 0.012	3.069ValIle: 3.069 ± 0.022	3.53ValLys: 3.53 ± 0.024	5.775ValLeu: 5.775 ± 0.026	1.344ValMet: 1.344 ± 0.011	2.368ValAsn: 2.368 ± 0.017	3.332ValPro: 3.332 ± 0.034	2.694ValGln: 2.694 ± 0.018	2.627ValArg: 2.627 ± 0.018	4.709ValSer: 4.709 ± 0.025	3.461ValThr: 3.461 ± 0.029	3.678ValVal: 3.678 ± 0.023	0.645ValTrp: 0.645 ± 0.009	1.582ValTyr: 1.582 ± 0.012	0.002ValXaa: 0.002 ± 0.0
Trp	0.653TrpAla: 0.653 ± 0.008	0.235TrpCys: 0.235 ± 0.004	0.617TrpAsp: 0.617 ± 0.009	0.748TrpGlu: 0.748 ± 0.008	0.445TrpPhe: 0.445 ± 0.008	0.587TrpGly: 0.587 ± 0.008	0.296TrpHis: 0.296 ± 0.005	0.624TrpIle: 0.624 ± 0.007	0.859TrpLys: 0.859 ± 0.008	1.16TrpLeu: 1.16 ± 0.012	0.322TrpMet: 0.322 ± 0.005	0.586TrpAsn: 0.586 ± 0.009	0.461TrpPro: 0.461 ± 0.007	0.533TrpGln: 0.533 ± 0.006	0.637TrpArg: 0.637 ± 0.009	0.837TrpSer: 0.837 ± 0.011	0.614TrpThr: 0.614 ± 0.009	0.618TrpVal: 0.618 ± 0.007	0.185TrpTrp: 0.185 ± 0.004	0.317TrpTyr: 0.317 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.435TyrAla: 1.435 ± 0.012	0.716TyrCys: 0.716 ± 0.009	1.372TyrAsp: 1.372 ± 0.012	1.73TyrGlu: 1.73 ± 0.016	1.306TyrPhe: 1.306 ± 0.013	1.697TyrGly: 1.697 ± 0.015	0.798TyrHis: 0.798 ± 0.009	1.604TyrIle: 1.604 ± 0.015	1.704TyrLys: 1.704 ± 0.022	2.739TyrLeu: 2.739 ± 0.017	0.643TyrMet: 0.643 ± 0.008	1.272TyrAsn: 1.272 ± 0.013	1.343TyrPro: 1.343 ± 0.012	1.331TyrGln: 1.331 ± 0.013	1.539TyrArg: 1.539 ± 0.012	2.338TyrSer: 2.338 ± 0.015	1.517TyrThr: 1.517 ± 0.015	1.499TyrVal: 1.499 ± 0.013	0.362TyrTrp: 0.362 ± 0.008	0.993TyrTyr: 0.993 ± 0.011	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.002XaaGlu: 0.002 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.004XaaGly: 0.004 ± 0.001	0.002XaaHis: 0.002 ± 0.0	0.003XaaIle: 0.003 ± 0.0	0.003XaaLys: 0.003 ± 0.0	0.003XaaLeu: 0.003 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.002XaaSer: 0.002 ± 0.0	0.002XaaThr: 0.002 ± 0.0	0.003XaaVal: 0.003 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.002XaaTyr: 0.002 ± 0.0	0.005XaaXaa: 0.005 ± 0.002

Statistics based on 22897 proteins (12268805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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