Tomato apical leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Topilevirus

Average proteome isoelectric point is 8.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4Z9M5|A0A2H4Z9M5_9GEMI C3 OS=Tomato apical leaf curl virus OX=2060142 GN=C3 PE=4 SV=1
MM1 pKa = 7.71PRR3 pKa = 11.84TPNTFRR9 pKa = 11.84LQGKK13 pKa = 9.52SIFLTYY19 pKa = 9.14PKK21 pKa = 10.35CPLIPLFVIDD31 pKa = 6.0YY32 pKa = 9.56LFQLLNDD39 pKa = 4.15FKK41 pKa = 10.17ITYY44 pKa = 10.32ARR46 pKa = 11.84VCQEE50 pKa = 3.52THH52 pKa = 6.17QDD54 pKa = 3.71GEE56 pKa = 4.45LHH58 pKa = 6.09LHH60 pKa = 6.45CLVQLEE66 pKa = 4.86EE67 pKa = 4.49KK68 pKa = 10.63FSTRR72 pKa = 11.84NPRR75 pKa = 11.84FFDD78 pKa = 2.99ISDD81 pKa = 3.84PNRR84 pKa = 11.84EE85 pKa = 4.17STYY88 pKa = 10.62HH89 pKa = 6.79PNAQIPRR96 pKa = 11.84RR97 pKa = 11.84DD98 pKa = 3.62ADD100 pKa = 3.59VADD103 pKa = 4.82YY104 pKa = 10.23IAKK107 pKa = 9.99GGVYY111 pKa = 9.12EE112 pKa = 4.12EE113 pKa = 4.74RR114 pKa = 11.84GLLRR118 pKa = 11.84ASRR121 pKa = 11.84RR122 pKa = 11.84SPKK125 pKa = 9.96KK126 pKa = 10.4SRR128 pKa = 11.84DD129 pKa = 3.96TIWGKK134 pKa = 10.48IIEE137 pKa = 4.48EE138 pKa = 4.26STNAEE143 pKa = 4.26DD144 pKa = 5.04FLSRR148 pKa = 11.84CQRR151 pKa = 11.84EE152 pKa = 4.04QPYY155 pKa = 9.48TYY157 pKa = 10.01ATQLRR162 pKa = 11.84NLEE165 pKa = 3.91YY166 pKa = 9.73MANKK170 pKa = 9.58KK171 pKa = 8.28WPKK174 pKa = 9.26PSANFTPKK182 pKa = 10.11FSIFNGVPEE191 pKa = 4.99PIKK194 pKa = 10.91NWVEE198 pKa = 3.62QNICVVHH205 pKa = 7.04RR206 pKa = 11.84DD207 pKa = 4.56FILLDD212 pKa = 3.81VPQLTQNDD220 pKa = 3.91LDD222 pKa = 5.19FYY224 pKa = 11.51DD225 pKa = 5.42SMAQEE230 pKa = 4.69SIEE233 pKa = 4.11QILEE237 pKa = 4.05NPDD240 pKa = 2.95NFTNIRR246 pKa = 11.84PHH248 pKa = 7.21IYY250 pKa = 9.87NDD252 pKa = 3.63SGPGEE257 pKa = 4.1

Molecular weight: 30.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4Z9X4|A0A2H4Z9X4_9GEMI Putative ssDNA-dsDNA regulatory protein OS=Tomato apical leaf curl virus OX=2060142 GN=V3 PE=4 SV=1
MM1 pKa = 8.08DD2 pKa = 3.32WTFNAWVYY10 pKa = 10.63LFVFISTVIITVFNGVTSIQLLRR33 pKa = 11.84ISEE36 pKa = 4.1KK37 pKa = 10.38LQRR40 pKa = 11.84ISADD44 pKa = 3.14LRR46 pKa = 11.84GSISHH51 pKa = 6.93LAITIGVAEE60 pKa = 4.41GRR62 pKa = 11.84VFQPSQGGRR71 pKa = 11.84VLRR74 pKa = 11.84VGRR77 pKa = 11.84TVSVPNRR84 pKa = 11.84VQEE87 pKa = 4.0DD88 pKa = 4.42RR89 pKa = 11.84PTGGEE94 pKa = 4.21TQVSPP99 pKa = 4.41

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1211

99

332

201.8

23.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.872 ± 0.653

1.899 ± 0.361

4.789 ± 0.573

5.78 ± 1.14

4.872 ± 0.66

5.533 ± 1.018

1.982 ± 0.28

6.441 ± 0.482

6.193 ± 0.918

7.845 ± 0.445

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.239 ± 0.316

5.78 ± 0.472

5.615 ± 0.881

4.542 ± 0.713

6.936 ± 0.284

7.597 ± 0.932

6.524 ± 0.797

5.12 ± 0.979

1.817 ± 0.239

4.624 ± 0.546
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski