Pseudomonas phage PMBT14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Knuthellervirus; Pseudomonas virus PMBT14

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PIA5|A0A2I6PIA5_9CAUD Uncharacterized protein OS=Pseudomonas phage PMBT14 OX=2059855 PE=4 SV=1
MM1 pKa = 7.72IPQTQPTSNSCISACLAMLAGVPTEE26 pKa = 4.53DD27 pKa = 4.81AVQQFHH33 pKa = 7.36DD34 pKa = 4.25ALWVEE39 pKa = 4.65GKK41 pKa = 9.79PIRR44 pKa = 11.84WCLEE48 pKa = 3.57EE49 pKa = 5.92LGIQYY54 pKa = 10.44RR55 pKa = 11.84SVFYY59 pKa = 10.5EE60 pKa = 3.52GHH62 pKa = 5.02TCYY65 pKa = 10.46GGKK68 pKa = 10.18VYY70 pKa = 10.35LAKK73 pKa = 10.5VASLNTRR80 pKa = 11.84GGLHH84 pKa = 5.62QVIIDD89 pKa = 3.68CRR91 pKa = 11.84GEE93 pKa = 3.95QTVVLDD99 pKa = 3.85PAQGFEE105 pKa = 4.5DD106 pKa = 3.23KK107 pKa = 10.49FYY109 pKa = 11.08YY110 pKa = 10.17AWEE113 pKa = 4.06PTKK116 pKa = 11.01GDD118 pKa = 4.22DD119 pKa = 3.17EE120 pKa = 4.48WAVPLEE126 pKa = 4.07SWLIEE131 pKa = 4.32YY132 pKa = 9.95EE133 pKa = 4.55VVPHH137 pKa = 7.12DD138 pKa = 3.67

Molecular weight: 15.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PI64|A0A2I6PI64_9CAUD RNA helicase OS=Pseudomonas phage PMBT14 OX=2059855 PE=4 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84SNAKK7 pKa = 9.92PIRR10 pKa = 11.84PQNKK14 pKa = 7.71PRR16 pKa = 11.84IYY18 pKa = 8.15YY19 pKa = 9.42HH20 pKa = 6.61VYY22 pKa = 8.54EE23 pKa = 4.51VGRR26 pKa = 11.84AVPGYY31 pKa = 8.32WRR33 pKa = 11.84VRR35 pKa = 11.84PIEE38 pKa = 4.38AKK40 pKa = 9.42WSQLRR45 pKa = 11.84VKK47 pKa = 10.29EE48 pKa = 3.93RR49 pKa = 11.84EE50 pKa = 3.95ARR52 pKa = 11.84LKK54 pKa = 10.44ANQWCNRR61 pKa = 11.84QNQKK65 pKa = 10.27EE66 pKa = 4.37FILSS70 pKa = 3.48

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15103

39

1159

198.7

22.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.753 ± 0.488

0.973 ± 0.12

5.721 ± 0.146

6.707 ± 0.403

3.211 ± 0.151

7.323 ± 0.288

1.695 ± 0.188

5.297 ± 0.205

5.986 ± 0.377

8.965 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.15

4.251 ± 0.218

4.714 ± 0.261

4.198 ± 0.263

5.866 ± 0.221

5.734 ± 0.217

6.197 ± 0.384

6.78 ± 0.192

1.827 ± 0.118

3.125 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski