Kipferlia bialata

Taxonomy: cellular organisms; Eukaryota; Metamonada; Fornicata; Carpediemonas-like organisms; Kipferlia

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A391NV19|A0A391NV19_9EUKA Peptidase C1A (Fragment) OS=Kipferlia bialata OX=797122 GN=KIPB_015686 PE=3 SV=1
DDD2 pKa = 5.7DDD4 pKa = 4.09TTCTVPGYYY13 pKa = 9.54VHHH16 pKa = 7.49DD17 pKa = 4.06ASVAGINVSGHHH29 pKa = 6.68VLGWDDD35 pKa = 3.62AGLDDD40 pKa = 3.81TGCVLDDD47 pKa = 4.32VADDD51 pKa = 4.66SQEEE55 pKa = 4.14IRR57 pKa = 11.84SAACVQKKK65 pKa = 10.85SFAGVCMRR73 pKa = 11.84GWDDD77 pKa = 4.09SGMDDD82 pKa = 3.91TGVSLKKK89 pKa = 10.99DD90 pKa = 3.31DDD92 pKa = 4.0TGVDDD97 pKa = 3.55TGATLTGACLTGTILEEE114 pKa = 4.45AYYY117 pKa = 9.16TDDD120 pKa = 3.58VIDDD124 pKa = 4.26VTGLSGDDD132 pKa = 3.64LAKKK136 pKa = 10.43SSIDDD141 pKa = 3.31VVFTRR146 pKa = 11.84FEEE149 pKa = 4.44KKK151 pKa = 10.45VCLGGLDDD159 pKa = 3.96SKKK162 pKa = 11.18DDD164 pKa = 3.6TGATLTGDDD173 pKa = 3.84LLGATGLTCAQVVSMEEE190 pKa = 5.08ITSLNLSSQDDD201 pKa = 3.35TDDD204 pKa = 3.3DDD206 pKa = 4.09SGRR209 pKa = 11.84TLTGCNLTNAIIEEE223 pKa = 4.13TLFKKK228 pKa = 11.01DD229 pKa = 3.43DDD231 pKa = 3.97SDDD234 pKa = 3.57TIGDDD239 pKa = 3.8TGLTIDDD246 pKa = 3.42L

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A391NIP5|A0A391NIP5_9EUKA Uncharacterized protein OS=Kipferlia bialata OX=797122 GN=KIPB_001368 PE=4 SV=1
MM1 pKa = 7.25HH2 pKa = 7.32HH3 pKa = 7.11GIHH6 pKa = 6.5HH7 pKa = 6.03GVGRR11 pKa = 11.84HH12 pKa = 4.3TSMARR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84TGIGMHH27 pKa = 6.17HH28 pKa = 6.84HH29 pKa = 6.28HH30 pKa = 6.61TT31 pKa = 3.88

Molecular weight: 3.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3513

0

3513

600654

8

9108

171.0

18.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.548 ± 0.07

2.118 ± 0.04

5.951 ± 0.059

6.756 ± 0.079

2.495 ± 0.044

7.711 ± 0.073

2.5 ± 0.041

3.593 ± 0.032

3.472 ± 0.071

8.971 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.029

2.265 ± 0.029

5.785 ± 0.06

3.589 ± 0.04

6.122 ± 0.096

8.455 ± 0.099

6.824 ± 0.081

8.34 ± 0.066

1.145 ± 0.021

2.789 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski